JP2014521312A5 - - Google Patents

Download PDF

Info

Publication number
JP2014521312A5
JP2014521312A5 JP2014519377A JP2014519377A JP2014521312A5 JP 2014521312 A5 JP2014521312 A5 JP 2014521312A5 JP 2014519377 A JP2014519377 A JP 2014519377A JP 2014519377 A JP2014519377 A JP 2014519377A JP 2014521312 A5 JP2014521312 A5 JP 2014521312A5
Authority
JP
Japan
Prior art keywords
nucleotide sequence
chemical group
double
ligase
group comprises
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2014519377A
Other languages
English (en)
Japanese (ja)
Other versions
JP6069314B2 (ja
JP2014521312A (ja
Filing date
Publication date
Priority claimed from CN2011101936354A external-priority patent/CN102876658A/zh
Application filed filed Critical
Publication of JP2014521312A publication Critical patent/JP2014521312A/ja
Publication of JP2014521312A5 publication Critical patent/JP2014521312A5/ja
Application granted granted Critical
Publication of JP6069314B2 publication Critical patent/JP6069314B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2014519377A 2011-07-12 2012-06-13 長鎖核酸分子の大規模合成方法 Expired - Fee Related JP6069314B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201110193635.4 2011-07-12
CN2011101936354A CN102876658A (zh) 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法
PCT/CN2012/000818 WO2013007099A1 (zh) 2011-07-12 2012-06-13 一种大规模合成长链核酸分子的方法

Publications (3)

Publication Number Publication Date
JP2014521312A JP2014521312A (ja) 2014-08-28
JP2014521312A5 true JP2014521312A5 (enExample) 2015-07-16
JP6069314B2 JP6069314B2 (ja) 2017-02-01

Family

ID=47478177

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014519377A Expired - Fee Related JP6069314B2 (ja) 2011-07-12 2012-06-13 長鎖核酸分子の大規模合成方法

Country Status (5)

Country Link
US (1) US9695417B2 (enExample)
EP (1) EP2733208B1 (enExample)
JP (1) JP6069314B2 (enExample)
CN (1) CN102876658A (enExample)
WO (1) WO2013007099A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102584955A (zh) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 一种转录激活子样效应因子的固相合成方法
WO2019073072A1 (en) * 2017-10-13 2019-04-18 Ribbon Biolabs Gmbh A NOVEL METHOD OF SYNTHESIZING POLYNUCLEOTIDES USING A DIVERSIFIED LIBRARY OF OLIGONUCLEOTIDES
CN109825431A (zh) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 寡核苷酸原位组装仪
CN113041967B (zh) * 2021-02-26 2022-09-27 通用生物(安徽)股份有限公司 一种超长链核酸合成生产工艺
CN119932137A (zh) * 2023-11-01 2025-05-06 清华大学 一种制备单链rna-dna嵌合体的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2007040592A1 (en) 2005-03-10 2007-04-12 Gao, Xiaolian Making nucleic acid sequences in parallel and use
WO2007091064A1 (en) * 2006-02-08 2007-08-16 Solexa Limited End modification to prevent over-representation of fragments

Similar Documents

Publication Publication Date Title
Van Dijk et al. Library preparation methods for next-generation sequencing: tone down the bias
CN109310784B (zh) 用于制备和使用指导核酸的方法和组合物
ES2827307T3 (es) Métodos y composiciones para la síntesis de ADNc y la elaboración del perfil del transcriptoma de células individuales utilizando una reacción de cambio de plantilla
WO2009102896A3 (en) Isothermal nucleic acid amplification methods and compositions
WO2006135684A3 (en) Methods and kits for sense rna synthesis
JP2018538006A5 (enExample)
WO2006029350A8 (en) Methods and compositions for tailing and amplifying rna
WO2006081284A3 (en) Methods for quantitating small rna molecules
JP2010535480A5 (enExample)
ATE464381T1 (de) Rna-amplifikation auf ligationsbasis
JP2010525813A5 (enExample)
WO2010090857A8 (en) Methods for amplifying hepatitis c virus nucleic acids
DE602007013223D1 (de) Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
JP2014521312A5 (enExample)
WO2012092569A3 (en) Compositions comprising immunostimulatory nucleic acids and related methods
ATE544871T1 (de) Hochmultiplexe nukleinsäureamplifikation
EP3511426A1 (en) Asymmetric hairpin target capture oligomers
JP2013507934A5 (enExample)
JP2016540517A5 (enExample)
JP6069314B2 (ja) 長鎖核酸分子の大規模合成方法
WO2008108843A3 (en) Methods and kits for amplifying dna
JP2006521824A5 (enExample)
WO2012083845A1 (zh) 用于除去测序文库中载体片段的方法及其用途
EP4010493B1 (en) Methods for the multiplexed isothermal amplification of nucleic acid sequences
DE602005017252D1 (de) Verfahren zur amplifikation von nukleinsäuren mittels promotor-templates