JP6069314B2 - 長鎖核酸分子の大規模合成方法 - Google Patents

長鎖核酸分子の大規模合成方法 Download PDF

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JP6069314B2
JP6069314B2 JP2014519377A JP2014519377A JP6069314B2 JP 6069314 B2 JP6069314 B2 JP 6069314B2 JP 2014519377 A JP2014519377 A JP 2014519377A JP 2014519377 A JP2014519377 A JP 2014519377A JP 6069314 B2 JP6069314 B2 JP 6069314B2
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nucleic acid
nucleotide sequence
double
acid molecules
short
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JP2014521312A5 (enExample
JP2014521312A (ja
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マー，チアンジュン
リー，シャオルー
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ペキン ビューソリッド バイオテック カンパニー，リミテッド
ペキン ビューソリッド バイオテック カンパニー，リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
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  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014519377A 2011-07-12 2012-06-13 長鎖核酸分子の大規模合成方法 Expired - Fee Related JP6069314B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201110193635.4 2011-07-12
CN2011101936354A CN102876658A (zh) 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法
PCT/CN2012/000818 WO2013007099A1 (zh) 2011-07-12 2012-06-13 一种大规模合成长链核酸分子的方法

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JP2014521312A JP2014521312A (ja) 2014-08-28
JP2014521312A5 JP2014521312A5 (enExample) 2015-07-16
JP6069314B2 true JP6069314B2 (ja) 2017-02-01

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JP2014519377A Expired - Fee Related JP6069314B2 (ja) 2011-07-12 2012-06-13 長鎖核酸分子の大規模合成方法

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US (1) US9695417B2 (enExample)
EP (1) EP2733208B1 (enExample)
JP (1) JP6069314B2 (enExample)
CN (1) CN102876658A (enExample)
WO (1) WO2013007099A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102584955A (zh) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 一种转录激活子样效应因子的固相合成方法
WO2019073072A1 (en) * 2017-10-13 2019-04-18 Ribbon Biolabs Gmbh A NOVEL METHOD OF SYNTHESIZING POLYNUCLEOTIDES USING A DIVERSIFIED LIBRARY OF OLIGONUCLEOTIDES
CN109825431A (zh) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 寡核苷酸原位组装仪
CN113041967B (zh) * 2021-02-26 2022-09-27 通用生物(安徽)股份有限公司 一种超长链核酸合成生产工艺
CN119932137A (zh) * 2023-11-01 2025-05-06 清华大学 一种制备单链rna-dna嵌合体的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2007040592A1 (en) 2005-03-10 2007-04-12 Gao, Xiaolian Making nucleic acid sequences in parallel and use
WO2007091064A1 (en) * 2006-02-08 2007-08-16 Solexa Limited End modification to prevent over-representation of fragments

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HK1198336A1 (en) 2015-06-19
EP2733208B1 (en) 2017-04-05
WO2013007099A1 (zh) 2013-01-17
EP2733208A4 (en) 2015-02-25
EP2733208A1 (en) 2014-05-21
CN102876658A (zh) 2013-01-16
US20140221255A1 (en) 2014-08-07
US9695417B2 (en) 2017-07-04
JP2014521312A (ja) 2014-08-28

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