CN102876658A - 一种大规模合成长链核酸分子的方法 - Google Patents

一种大规模合成长链核酸分子的方法 Download PDF

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Publication number
CN102876658A
CN102876658A CN2011101936354A CN201110193635A CN102876658A CN 102876658 A CN102876658 A CN 102876658A CN 2011101936354 A CN2011101936354 A CN 2011101936354A CN 201110193635 A CN201110193635 A CN 201110193635A CN 102876658 A CN102876658 A CN 102876658A
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China
Prior art keywords
nucleic acid
acid molecule
chain nucleic
long
chain
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Pending
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CN2011101936354A
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English (en)
Chinese (zh)
Inventor
马千君
李绍路
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BEIJING VIEWSOLID BIOTECH Co Ltd
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BEIJING VIEWSOLID BIOTECH Co Ltd
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Application filed by BEIJING VIEWSOLID BIOTECH Co Ltd filed Critical BEIJING VIEWSOLID BIOTECH Co Ltd
Priority to CN2011101936354A priority Critical patent/CN102876658A/zh
Priority to US14/232,542 priority patent/US9695417B2/en
Priority to PCT/CN2012/000818 priority patent/WO2013007099A1/zh
Priority to HK14111273.9A priority patent/HK1198336B/en
Priority to JP2014519377A priority patent/JP6069314B2/ja
Priority to EP12810692.9A priority patent/EP2733208B1/en
Publication of CN102876658A publication Critical patent/CN102876658A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011101936354A 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法 Pending CN102876658A (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN2011101936354A CN102876658A (zh) 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法
US14/232,542 US9695417B2 (en) 2011-07-12 2012-06-13 Method for large-scale synthesis of long-chain nucleic acid molecule
PCT/CN2012/000818 WO2013007099A1 (zh) 2011-07-12 2012-06-13 一种大规模合成长链核酸分子的方法
HK14111273.9A HK1198336B (en) 2011-07-12 2012-06-13 Method for large-scale synthesis of long-chain nucleic acid molecule
JP2014519377A JP6069314B2 (ja) 2011-07-12 2012-06-13 長鎖核酸分子の大規模合成方法
EP12810692.9A EP2733208B1 (en) 2011-07-12 2012-06-13 Method for large-scale synthesis of long-chain nucleic acid molecule

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011101936354A CN102876658A (zh) 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法

Publications (1)

Publication Number Publication Date
CN102876658A true CN102876658A (zh) 2013-01-16

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CN2011101936354A Pending CN102876658A (zh) 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法

Country Status (5)

Country Link
US (1) US9695417B2 (enExample)
EP (1) EP2733208B1 (enExample)
JP (1) JP6069314B2 (enExample)
CN (1) CN102876658A (enExample)
WO (1) WO2013007099A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102584955A (zh) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 一种转录激活子样效应因子的固相合成方法
CN109825431A (zh) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 寡核苷酸原位组装仪
CN111527205A (zh) * 2017-10-13 2020-08-11 里本生物实验室有限责任公司 使用寡核苷酸的多样性文库合成多核苷酸的新方法
WO2025091594A1 (zh) * 2023-11-01 2025-05-08 清华大学 一种制备单链rna-dna嵌合体的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113041967B (zh) * 2021-02-26 2022-09-27 通用生物(安徽)股份有限公司 一种超长链核酸合成生产工艺

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007086935A2 (en) * 2005-08-01 2007-08-02 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2007091064A1 (en) * 2006-02-08 2007-08-16 Solexa Limited End modification to prevent over-representation of fragments
CN101133166B (zh) * 2005-03-10 2011-02-02 高晓莲 平行制备核酸及应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101133166B (zh) * 2005-03-10 2011-02-02 高晓莲 平行制备核酸及应用
WO2007086935A2 (en) * 2005-08-01 2007-08-02 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2007091064A1 (en) * 2006-02-08 2007-08-16 Solexa Limited End modification to prevent over-representation of fragments

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102584955A (zh) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 一种转录激活子样效应因子的固相合成方法
CN111527205A (zh) * 2017-10-13 2020-08-11 里本生物实验室有限责任公司 使用寡核苷酸的多样性文库合成多核苷酸的新方法
US12018251B2 (en) 2017-10-13 2024-06-25 Ribbon Biolabs Gmbh Method for synthesis of polynucleotides using a diverse library of oligonucleotides
CN109825431A (zh) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 寡核苷酸原位组装仪
WO2025091594A1 (zh) * 2023-11-01 2025-05-08 清华大学 一种制备单链rna-dna嵌合体的方法

Also Published As

Publication number Publication date
EP2733208A4 (en) 2015-02-25
EP2733208A1 (en) 2014-05-21
US20140221255A1 (en) 2014-08-07
HK1198336A1 (en) 2015-06-19
EP2733208B1 (en) 2017-04-05
JP2014521312A (ja) 2014-08-28
WO2013007099A1 (zh) 2013-01-17
US9695417B2 (en) 2017-07-04
JP6069314B2 (ja) 2017-02-01

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Application publication date: 20130116