JP2013515458A5 - - Google Patents
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- Publication number
- JP2013515458A5 JP2013515458A5 JP2012537100A JP2012537100A JP2013515458A5 JP 2013515458 A5 JP2013515458 A5 JP 2013515458A5 JP 2012537100 A JP2012537100 A JP 2012537100A JP 2012537100 A JP2012537100 A JP 2012537100A JP 2013515458 A5 JP2013515458 A5 JP 2013515458A5
- Authority
- JP
- Japan
- Prior art keywords
- mycoplasma
- seq
- amplification reaction
- primer
- binding domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 claims description 62
- 230000003321 amplification Effects 0.000 claims description 44
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 44
- 238000006243 chemical reaction Methods 0.000 claims description 33
- 230000004568 DNA-binding Effects 0.000 claims description 18
- 108020004707 nucleic acids Proteins 0.000 claims description 16
- 102000039446 nucleic acids Human genes 0.000 claims description 16
- 150000007523 nucleic acids Chemical class 0.000 claims description 16
- 241000204031 Mycoplasma Species 0.000 claims description 14
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 12
- 241000204003 Mycoplasmatales Species 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 230000000065 osmolyte Effects 0.000 claims description 12
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims description 12
- UYPYRKYUKCHHIB-UHFFFAOYSA-N trimethylamine N-oxide Chemical compound C[N+](C)(C)[O-] UYPYRKYUKCHHIB-UHFFFAOYSA-N 0.000 claims description 12
- 239000006143 cell culture medium Substances 0.000 claims description 10
- 108020004414 DNA Proteins 0.000 claims description 8
- 102000053602 DNA Human genes 0.000 claims description 6
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 6
- DFPOZTRSOAQFIK-UHFFFAOYSA-N S,S-dimethyl-beta-propiothetin Chemical compound C[S+](C)CCC([O-])=O DFPOZTRSOAQFIK-UHFFFAOYSA-N 0.000 claims description 6
- 108010077895 Sarcosine Proteins 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 6
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 6
- 229920000669 heparin Polymers 0.000 claims description 6
- 229960002897 heparin Drugs 0.000 claims description 6
- 239000003112 inhibitor Substances 0.000 claims description 6
- 101150090202 rpoB gene Proteins 0.000 claims description 6
- 229940043230 sarcosine Drugs 0.000 claims description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 4
- 108091034117 Oligonucleotide Proteins 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- 238000009830 intercalation Methods 0.000 claims description 4
- 108091033319 polynucleotide Proteins 0.000 claims description 4
- 102000040430 polynucleotide Human genes 0.000 claims description 4
- 239000002157 polynucleotide Substances 0.000 claims description 4
- 239000013641 positive control Substances 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 241000016224 Halanaerobium fermentans Species 0.000 claims description 2
- 241000384535 Monomorphina pyrum Species 0.000 claims description 2
- 241000204028 Mycoplasma arginini Species 0.000 claims description 2
- 241000202952 Mycoplasma fermentans Species 0.000 claims description 2
- 241000202938 Mycoplasma hyorhinis Species 0.000 claims description 2
- 241000202892 Mycoplasma pirum Species 0.000 claims description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 2
- 108010006785 Taq Polymerase Proteins 0.000 claims description 2
- 239000012091 fetal bovine serum Substances 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 239000000523 sample Substances 0.000 claims description 2
- 239000002699 waste material Substances 0.000 claims description 2
- 238000001514 detection method Methods 0.000 description 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25621609P | 2009-10-29 | 2009-10-29 | |
| US61/256,216 | 2009-10-29 | ||
| PCT/US2010/054658 WO2011059802A1 (en) | 2009-10-29 | 2010-10-29 | Rapid detection of mycoplasma contamination in cell culture samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013515458A JP2013515458A (ja) | 2013-05-09 |
| JP2013515458A5 true JP2013515458A5 (enExample) | 2014-01-23 |
Family
ID=43925840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012537100A Withdrawn JP2013515458A (ja) | 2009-10-29 | 2010-10-29 | 細胞培養試料中のマイコプラズマ汚染の迅速な検出 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20110104686A1 (enExample) |
| EP (1) | EP2494079A4 (enExample) |
| JP (1) | JP2013515458A (enExample) |
| CA (1) | CA2779255A1 (enExample) |
| WO (1) | WO2011059802A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| HK1215593A1 (zh) * | 2012-12-18 | 2016-09-02 | Merial, Inc. | Ibv、csfv和ndv的高分辨熔解基因分型 |
| US9217175B2 (en) | 2013-02-01 | 2015-12-22 | Bio-Rad Laboratories, Inc. | Multiplexed digital assay with specific and generic reporters |
| JP6704565B2 (ja) | 2014-09-10 | 2020-06-03 | 国立大学法人 東京医科歯科大学 | マイコプラズマを検出する方法 |
| EP3378948B1 (en) * | 2015-11-18 | 2020-11-04 | Japan Agency for Marine-Earth Science and Technology | Method for quantifying target nucleic acid and kit therefor |
| CN111433372B (zh) | 2017-10-19 | 2024-01-26 | 生物辐射实验室股份有限公司 | 具有光致发光的非常规和/或反向变化的数字扩增试验 |
| EP4143312A4 (en) * | 2020-05-01 | 2024-07-10 | Helix Nanotechnologies, Inc. | COMPOSITIONS AND METHODS FOR RNA SYNTHESIS |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1246935B1 (en) * | 1999-09-28 | 2013-08-14 | Geneohm Sciences Canada Inc. | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| US20040121311A1 (en) * | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in livestock |
| US7560260B2 (en) * | 2002-07-25 | 2009-07-14 | Bio-Rad Laboratories, Inc. | Compositions with polymerase activity |
| US7666645B2 (en) * | 2002-10-23 | 2010-02-23 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugate proteins |
| FI115637B (fi) * | 2003-12-19 | 2005-06-15 | Mobidiag Oy | Diagnostinen menetelmä hengitystieinfektioita aiheuttavien bakteerien osoittamiseksi ja tunnistamiseksi ja menetelmässä käyttökelpoinen alukeseos |
| AU2006287548A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| US7872116B2 (en) * | 2007-02-06 | 2011-01-18 | American Type Culture Collection (Atcc) | Identification of cell culture contaminants among Mollicutes species by a PCR based assay |
-
2010
- 2010-10-29 CA CA2779255A patent/CA2779255A1/en not_active Abandoned
- 2010-10-29 JP JP2012537100A patent/JP2013515458A/ja not_active Withdrawn
- 2010-10-29 WO PCT/US2010/054658 patent/WO2011059802A1/en not_active Ceased
- 2010-10-29 US US12/915,485 patent/US20110104686A1/en not_active Abandoned
- 2010-10-29 EP EP10830491.6A patent/EP2494079A4/en not_active Withdrawn
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