CA2779255A1 - Rapid detection of mycoplasma contamination in cell culture samples - Google Patents
Rapid detection of mycoplasma contamination in cell culture samples Download PDFInfo
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- CA2779255A1 CA2779255A1 CA2779255A CA2779255A CA2779255A1 CA 2779255 A1 CA2779255 A1 CA 2779255A1 CA 2779255 A CA2779255 A CA 2779255A CA 2779255 A CA2779255 A CA 2779255A CA 2779255 A1 CA2779255 A1 CA 2779255A1
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- Prior art keywords
- mycoplasma
- seq
- amplification
- primer
- amplification reaction
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Applications Claiming Priority (3)
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| US25621609P | 2009-10-29 | 2009-10-29 | |
| US61/256,216 | 2009-10-29 | ||
| PCT/US2010/054658 WO2011059802A1 (en) | 2009-10-29 | 2010-10-29 | Rapid detection of mycoplasma contamination in cell culture samples |
Publications (1)
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| CA2779255A1 true CA2779255A1 (en) | 2011-05-19 |
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Family Applications (1)
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|---|---|---|---|
| CA2779255A Abandoned CA2779255A1 (en) | 2009-10-29 | 2010-10-29 | Rapid detection of mycoplasma contamination in cell culture samples |
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| US (1) | US20110104686A1 (enExample) |
| EP (1) | EP2494079A4 (enExample) |
| JP (1) | JP2013515458A (enExample) |
| CA (1) | CA2779255A1 (enExample) |
| WO (1) | WO2011059802A1 (enExample) |
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| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CA2895324A1 (en) * | 2012-12-18 | 2014-06-26 | Merial, Inc. | High resolution melt genotyping of ibv, csfv and ndv |
| US9523116B2 (en) | 2013-02-01 | 2016-12-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assay with data exclusion for calculation of target levels |
| JP6704565B2 (ja) | 2014-09-10 | 2020-06-03 | 国立大学法人 東京医科歯科大学 | マイコプラズマを検出する方法 |
| JP6284137B2 (ja) * | 2015-11-18 | 2018-02-28 | 国立研究開発法人海洋研究開発機構 | 標的核酸の定量方法及びそのためのキット |
| CN111433372B (zh) | 2017-10-19 | 2024-01-26 | 生物辐射实验室股份有限公司 | 具有光致发光的非常规和/或反向变化的数字扩增试验 |
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| CA2937907C (en) * | 1999-09-28 | 2017-08-22 | Geneohm Sciences Canada Inc. | Nucleic acids, methods and kits for the detection of campylobacter |
| US20040121309A1 (en) * | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues |
| US7560260B2 (en) * | 2002-07-25 | 2009-07-14 | Bio-Rad Laboratories, Inc. | Compositions with polymerase activity |
| US7666645B2 (en) * | 2002-10-23 | 2010-02-23 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugate proteins |
| FI115637B (fi) * | 2003-12-19 | 2005-06-15 | Mobidiag Oy | Diagnostinen menetelmä hengitystieinfektioita aiheuttavien bakteerien osoittamiseksi ja tunnistamiseksi ja menetelmässä käyttökelpoinen alukeseos |
| AU2006287548A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| US7872116B2 (en) * | 2007-02-06 | 2011-01-18 | American Type Culture Collection (Atcc) | Identification of cell culture contaminants among Mollicutes species by a PCR based assay |
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2010
- 2010-10-29 WO PCT/US2010/054658 patent/WO2011059802A1/en not_active Ceased
- 2010-10-29 EP EP10830491.6A patent/EP2494079A4/en not_active Withdrawn
- 2010-10-29 JP JP2012537100A patent/JP2013515458A/ja not_active Withdrawn
- 2010-10-29 US US12/915,485 patent/US20110104686A1/en not_active Abandoned
- 2010-10-29 CA CA2779255A patent/CA2779255A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
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| US20110104686A1 (en) | 2011-05-05 |
| EP2494079A4 (en) | 2013-06-26 |
| WO2011059802A1 (en) | 2011-05-19 |
| JP2013515458A (ja) | 2013-05-09 |
| EP2494079A1 (en) | 2012-09-05 |
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| Date | Code | Title | Description |
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| FZDE | Dead |
Effective date: 20151029 |