JP2016532435A5 - - Google Patents
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- JP2016532435A5 JP2016532435A5 JP2016519572A JP2016519572A JP2016532435A5 JP 2016532435 A5 JP2016532435 A5 JP 2016532435A5 JP 2016519572 A JP2016519572 A JP 2016519572A JP 2016519572 A JP2016519572 A JP 2016519572A JP 2016532435 A5 JP2016532435 A5 JP 2016532435A5
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361833092P | 2013-06-10 | 2013-06-10 | |
| US61/833,092 | 2013-06-10 | ||
| PCT/US2014/041513 WO2014200905A2 (en) | 2013-06-10 | 2014-06-09 | Early developmental genomic assay for characterizing pluripotent stem cell utility and safety |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016532435A JP2016532435A (ja) | 2016-10-20 |
| JP2016532435A5 true JP2016532435A5 (enExample) | 2017-07-20 |
| JP6697380B2 JP6697380B2 (ja) | 2020-05-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016519572A Active JP6697380B2 (ja) | 2013-06-10 | 2014-06-09 | 多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US11085067B2 (enExample) |
| EP (1) | EP3008229B1 (enExample) |
| JP (1) | JP6697380B2 (enExample) |
| WO (1) | WO2014200905A2 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015193951A1 (ja) | 2014-06-16 | 2015-12-23 | 株式会社ニコン | 観察装置、観察方法、観察システム、そのプログラム、および細胞の製造方法 |
| CN117201777A (zh) | 2016-09-13 | 2023-12-08 | 韩国电子通信研究院 | 视频编码/解码方法和装置以及存储比特流的记录介质 |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| US11603563B2 (en) * | 2017-06-10 | 2023-03-14 | Shimadzu Corporation | Method of predicting differentiation potential of iPS cells into cartilage cells based on gene expression profiles |
| AU2018324301A1 (en) * | 2017-09-01 | 2020-04-16 | Orig3N, Inc. | I-PSC derived secretome compositions, and related systems and methods |
| WO2019078342A1 (ja) * | 2017-10-20 | 2019-04-25 | 国立大学法人大阪大学 | 心筋細胞への分化指向性を有する多能性幹細胞を選抜するための方法 |
| CN113646424A (zh) * | 2019-03-29 | 2021-11-12 | 富士胶片株式会社 | 具有分化成特定细胞的能力的多能干细胞的制造方法及其应用 |
| WO2021095798A1 (ja) * | 2019-11-15 | 2021-05-20 | 公立大学法人横浜市立大学 | 未分化マーカー遺伝子高感度検出法 |
| JPWO2021145402A1 (enExample) * | 2020-01-16 | 2021-07-22 | ||
| US10968493B1 (en) * | 2020-03-12 | 2021-04-06 | New England Biolabs, Inc. | Rapid diagnostic test using colorimetric LAMP |
| JPWO2021256078A1 (enExample) * | 2020-06-19 | 2021-12-23 | ||
| EP3940704A1 (en) * | 2020-07-14 | 2022-01-19 | Koninklijke Philips N.V. | Method for determining the differentiation state of a stem cell |
| CN112575010B (zh) * | 2020-12-14 | 2022-11-08 | 云南农业大学 | 山药不同组织荧光定量的内参基因及其引物与应用 |
| CN119137667A (zh) * | 2022-03-25 | 2024-12-13 | 宾州研究基金会 | 针对基因组甲基化数据的基因组词框架分析 |
| WO2023201361A1 (en) * | 2022-04-15 | 2023-10-19 | Aspen Neuroscience, Inc. | Methods of classifying the differentiation state of cells and related compositions of differentiated cells |
Family Cites Families (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US548257A (en) | 1895-10-22 | Hay rake and loader | ||
| US4963489A (en) | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| US6270961B1 (en) | 1987-04-01 | 2001-08-07 | Hyseq, Inc. | Methods and apparatus for DNA sequencing and DNA identification |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| CA2040503A1 (en) | 1990-04-25 | 1991-10-26 | James E. Stefano | Selective amplification system using q beta replicase |
| DE69132905T2 (de) | 1990-12-06 | 2002-08-01 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Detektion von Nukleinsäuresequenzen |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| AU4543193A (en) | 1992-06-22 | 1994-01-24 | Henry E. Young | Scar inhibitory factor and use thereof |
| US5672346A (en) | 1992-07-27 | 1997-09-30 | Indiana University Foundation | Human stem cell compositions and methods |
| CA2146747C (en) | 1992-10-09 | 2006-12-19 | Brian A. Naughton | Liver reserve cells |
| US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| US6136540A (en) | 1994-10-03 | 2000-10-24 | Ikonisys Inc. | Automated fluorescence in situ hybridization detection of genetic abnormalities |
| US5952172A (en) | 1993-12-10 | 1999-09-14 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US6379897B1 (en) | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
| US5622826A (en) | 1994-12-22 | 1997-04-22 | Houston Advanced Research Center | Method for immobilization of molecules on platinum solid support surfaces |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| US5968740A (en) | 1995-07-24 | 1999-10-19 | Affymetrix, Inc. | Method of Identifying a Base in a Nucleic Acid |
| US5569588A (en) | 1995-08-09 | 1996-10-29 | The Regents Of The University Of California | Methods for drug screening |
| US5994619A (en) | 1996-04-01 | 1999-11-30 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Production of chimeric bovine or porcine animals using cultured inner cell mass cells |
| EP0990142A4 (en) | 1996-12-31 | 2000-09-27 | Genometrix Genomics Inc | MULTIPLEXED MOLECULAR ANALYSIS METHOD AND DEVICE |
| US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US6465611B1 (en) | 1997-02-25 | 2002-10-15 | Corixa Corporation | Compounds for immunotherapy of prostate cancer and methods for their use |
| US6642433B1 (en) | 1997-05-15 | 2003-11-04 | Trillium Therapeutics Inc. | Fgl-2 knockout mice |
| CA2316912C (en) | 1998-01-12 | 2009-09-15 | Massachusetts Institute Of Technology | Method and apparatus for performing microassays |
| US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
| US6235473B1 (en) | 1998-07-02 | 2001-05-22 | Orchid Biosciences, Inc. | Gene pen devices for array printing |
| WO2001055454A1 (en) | 2000-01-28 | 2001-08-02 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| US7332271B2 (en) | 2000-02-18 | 2008-02-19 | Board Of Trustees Of The Leland Stanford Junior University | Apparatus and methods for parallel processing of micro-volume liquid reactions |
| KR20020097181A (ko) | 2000-02-22 | 2002-12-31 | 제노스펙트라 인코포레이티드 | 마이크로어레이 제작 기술 및 장치 |
| WO2001075162A2 (en) | 2000-03-31 | 2001-10-11 | University Of Louisville Research Foundation, Inc. | Microarrays to screen regulatory genes |
| AU8617301A (en) | 2000-08-01 | 2002-02-13 | Yissum Res Dev Co | Directed differentiation of embryonic cells |
| US6902702B1 (en) | 2000-08-16 | 2005-06-07 | University Health Network | Devices and methods for producing microarrays of biological samples |
| AU2001286171B2 (en) | 2001-05-25 | 2008-01-10 | Serono Genetics Institute S.A. | Human CDNAs and proteins and uses thereof |
| WO2003043402A2 (en) | 2001-10-19 | 2003-05-30 | Proligo Llc | Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes |
| US20030113910A1 (en) | 2001-12-18 | 2003-06-19 | Mike Levanduski | Pluripotent stem cells derived without the use of embryos or fetal tissue |
| US20030215858A1 (en) | 2002-04-08 | 2003-11-20 | Baylor College Of Medicine | Enhanced gene expression system |
| US20030194711A1 (en) | 2002-04-10 | 2003-10-16 | Matthew Zapala | System and method for analyzing gene expression data |
| US20040162679A1 (en) | 2002-05-03 | 2004-08-19 | Linheng Li | Method for predicting gene potential and cell commitment |
| WO2004009758A2 (en) | 2002-07-23 | 2004-01-29 | Nanodiagnostics, Inc. | Embryonic stem cell markers and uses thereof |
| US7153650B2 (en) | 2003-03-13 | 2006-12-26 | Geron Corporation | Marker system for preparing and characterizing high-quality human embryonic stem cells |
| US20060177825A1 (en) | 2003-04-29 | 2006-08-10 | Mcdonald John F | Global analysis of transposable elements as molecular markers of the developmental potential of stem cells |
| GB0413005D0 (en) | 2004-06-11 | 2004-07-14 | Coletica | Ligand |
| GB0504427D0 (en) * | 2005-03-03 | 2005-04-06 | Roslin Inst Edinburgh | Method for differentiation of stem cells |
| EP1948822B1 (en) | 2005-10-07 | 2011-07-13 | Johnson & Johnson Research Pty Limited | Multicomponent nucleic acid enzymes and methods for their use |
| CA2626152A1 (en) | 2005-10-24 | 2007-05-03 | Agency For Science, Technology And Research | Methods of specifying mesodermal, endodermal and mesoendodermal cell fates |
| US20090227032A1 (en) | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
| KR101420740B1 (ko) | 2005-12-13 | 2014-07-17 | 교또 다이가꾸 | 핵초기화 인자 |
| US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| EP2137296A2 (en) | 2007-03-23 | 2009-12-30 | Wisconsin Alumni Research Foundation | Somatic cell reprogramming |
| WO2008122084A1 (en) | 2007-04-05 | 2008-10-16 | Johnson & Johnson Research Pty Limited | Nucleic acid enzymes and complexes and methods for their use |
| CN105861443A (zh) | 2007-04-07 | 2016-08-17 | 怀特黑德生物医学研究所 | 体细胞重编程 |
| CN101802172A (zh) | 2007-05-30 | 2010-08-11 | 通用医疗公司 | 由体细胞产生多能细胞的方法 |
| US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| US7615374B2 (en) | 2007-09-25 | 2009-11-10 | Wisconsin Alumni Research Foundation | Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions |
| EP2283117B1 (en) | 2008-04-21 | 2013-10-23 | Viacyte, Inc. | Methods for purifying pancreatic endoderm cells derived from human embryonic stem cells |
| US20100003674A1 (en) | 2008-07-03 | 2010-01-07 | Cope Frederick O | Adult stem cells, molecular signatures, and applications in the evaluation, diagnosis, and therapy of mammalian conditions |
| EP2313494A2 (en) | 2008-07-14 | 2011-04-27 | Oklahoma Medical Research Foundation | Production of pluripotent cells through inhibition of bright/arid3a function |
| US8278105B2 (en) | 2008-09-09 | 2012-10-02 | University Of Southern California | Induction, propagation and isolation of liver progenitor cells |
| US20120021519A1 (en) | 2008-09-19 | 2012-01-26 | Presidents And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
| EP3450545B1 (en) | 2008-10-24 | 2023-08-23 | Wisconsin Alumni Research Foundation | Pluripotent stem cells obtained by non-viral reprogramming |
| WO2010111422A2 (en) | 2009-03-25 | 2010-09-30 | The Salk Institute For Biological Studies | Induced pluripotent stem cell generation using two factors and p53 inactivation |
| WO2011008541A2 (en) | 2009-06-29 | 2011-01-20 | The Regents Of The University Of California | Molecular markers and assay methods for characterizing cells |
| WO2011046635A1 (en) | 2009-10-14 | 2011-04-21 | The Johns Hopkins University | Differentially methylated regions of reprogrammed induced pluripotent stem cells, method and compositions thereof |
| WO2011130624A2 (en) | 2010-04-16 | 2011-10-20 | Immune Disease Institute, Inc. | Sustained polypeptide expression from synthetic, modified rnas and uses thereof |
| US20130259842A1 (en) | 2010-05-18 | 2013-10-03 | Lee Rubin | Stable reprogrammed cells |
| US8945847B2 (en) | 2010-05-24 | 2015-02-03 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Methods and kits for ascertaining biosafety of an agent |
| JP2013545439A (ja) | 2010-09-17 | 2013-12-26 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 多能性幹細胞の有用性および安全性の特徴決定を行うための機能的ゲノミクスアッセイ |
| US20160115455A1 (en) | 2013-05-17 | 2016-04-28 | The Broad Institute, Inc. | Reprogrammed cells and methods of production and use thereof |
-
2014
- 2014-06-09 JP JP2016519572A patent/JP6697380B2/ja active Active
- 2014-06-09 EP EP14811445.7A patent/EP3008229B1/en active Active
- 2014-06-09 US US14/897,410 patent/US11085067B2/en active Active
- 2014-06-09 WO PCT/US2014/041513 patent/WO2014200905A2/en not_active Ceased
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2018
- 2018-05-18 US US15/983,209 patent/US10626445B2/en active Active
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