JP6697380B2 - 多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ - Google Patents
多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ Download PDFInfo
- Publication number
- JP6697380B2 JP6697380B2 JP2016519572A JP2016519572A JP6697380B2 JP 6697380 B2 JP6697380 B2 JP 6697380B2 JP 2016519572 A JP2016519572 A JP 2016519572A JP 2016519572 A JP2016519572 A JP 2016519572A JP 6697380 B2 JP6697380 B2 JP 6697380B2
- Authority
- JP
- Japan
- Prior art keywords
- genes
- gene
- early
- stem cell
- pluripotent stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000001778 pluripotent stem cell Anatomy 0.000 title claims description 535
- 238000003556 assay Methods 0.000 title claims description 202
- 108090000623 proteins and genes Proteins 0.000 claims description 859
- 230000014509 gene expression Effects 0.000 claims description 525
- 210000000130 stem cell Anatomy 0.000 claims description 384
- 108700029231 Developmental Genes Proteins 0.000 claims description 333
- 238000011161 development Methods 0.000 claims description 292
- 230000018109 developmental process Effects 0.000 claims description 290
- 238000000034 method Methods 0.000 claims description 270
- 230000004069 differentiation Effects 0.000 claims description 178
- 210000001900 endoderm Anatomy 0.000 claims description 109
- 239000000523 sample Substances 0.000 claims description 108
- 150000007523 nucleic acids Chemical class 0.000 claims description 97
- 108091034117 Oligonucleotide Proteins 0.000 claims description 96
- 210000003716 mesoderm Anatomy 0.000 claims description 86
- 108020004999 messenger RNA Proteins 0.000 claims description 79
- 210000003981 ectoderm Anatomy 0.000 claims description 77
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 68
- 102000039446 nucleic acids Human genes 0.000 claims description 67
- 108020004707 nucleic acids Proteins 0.000 claims description 67
- 230000003321 amplification Effects 0.000 claims description 66
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 66
- -1 HAND2 Proteins 0.000 claims description 49
- 239000007787 solid Substances 0.000 claims description 31
- 239000003153 chemical reaction reagent Substances 0.000 claims description 29
- 239000002299 complementary DNA Substances 0.000 claims description 23
- 102100029284 Hepatocyte nuclear factor 3-beta Human genes 0.000 claims description 19
- 102100038146 Homeobox protein goosecoid Human genes 0.000 claims description 19
- 101001062347 Homo sapiens Hepatocyte nuclear factor 3-beta Proteins 0.000 claims description 19
- 101001032602 Homo sapiens Homeobox protein goosecoid Proteins 0.000 claims description 19
- 230000003847 mesoderm development Effects 0.000 claims description 19
- 102100023855 Heart- and neural crest derivatives-expressed protein 1 Human genes 0.000 claims description 18
- 101000905239 Homo sapiens Heart- and neural crest derivatives-expressed protein 1 Proteins 0.000 claims description 18
- 108020004635 Complementary DNA Proteins 0.000 claims description 17
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 claims description 17
- 102100030751 Eomesodermin homolog Human genes 0.000 claims description 16
- 101001064167 Homo sapiens Eomesodermin homolog Proteins 0.000 claims description 16
- 101001038339 Homo sapiens LIM homeobox transcription factor 1-alpha Proteins 0.000 claims description 16
- 102100040290 LIM homeobox transcription factor 1-alpha Human genes 0.000 claims description 16
- 102100028726 Bone morphogenetic protein 10 Human genes 0.000 claims description 15
- 102100025332 Cadherin-9 Human genes 0.000 claims description 15
- 102100021860 Endothelial cell-specific molecule 1 Human genes 0.000 claims description 15
- 102100024520 Ficolin-3 Human genes 0.000 claims description 15
- 102100034633 Homeobox expressed in ES cells 1 Human genes 0.000 claims description 15
- 102100028798 Homeodomain-only protein Human genes 0.000 claims description 15
- 101000695367 Homo sapiens Bone morphogenetic protein 10 Proteins 0.000 claims description 15
- 101000935098 Homo sapiens Cadherin-9 Proteins 0.000 claims description 15
- 101000897959 Homo sapiens Endothelial cell-specific molecule 1 Proteins 0.000 claims description 15
- 101001052749 Homo sapiens Ficolin-3 Proteins 0.000 claims description 15
- 101001067288 Homo sapiens Homeobox expressed in ES cells 1 Proteins 0.000 claims description 15
- 101000839095 Homo sapiens Homeodomain-only protein Proteins 0.000 claims description 15
- 101001037256 Homo sapiens Indoleamine 2,3-dioxygenase 1 Proteins 0.000 claims description 15
- 101000967920 Homo sapiens Left-right determination factor 1 Proteins 0.000 claims description 15
- 101000603107 Homo sapiens Noelin-3 Proteins 0.000 claims description 15
- 101001094741 Homo sapiens POU domain, class 4, transcription factor 1 Proteins 0.000 claims description 15
- 101001094700 Homo sapiens POU domain, class 5, transcription factor 1 Proteins 0.000 claims description 15
- 101000781955 Homo sapiens Proto-oncogene Wnt-1 Proteins 0.000 claims description 15
- 101001047681 Homo sapiens Tyrosine-protein kinase Lck Proteins 0.000 claims description 15
- 102100040061 Indoleamine 2,3-dioxygenase 1 Human genes 0.000 claims description 15
- 102100040508 Left-right determination factor 1 Human genes 0.000 claims description 15
- 102100037046 Noelin-3 Human genes 0.000 claims description 15
- 102100035395 POU domain, class 4, transcription factor 1 Human genes 0.000 claims description 15
- 102000003610 TRPM8 Human genes 0.000 claims description 15
- 101150111302 Trpm8 gene Proteins 0.000 claims description 15
- 102100024036 Tyrosine-protein kinase Lck Human genes 0.000 claims description 15
- 102000052547 Wnt-1 Human genes 0.000 claims description 15
- 239000002773 nucleotide Substances 0.000 claims description 15
- 125000003729 nucleotide group Chemical group 0.000 claims description 15
- 230000020619 endoderm development Effects 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 13
- 102100022900 Actin, cytoplasmic 1 Human genes 0.000 claims description 12
- 108010085238 Actins Proteins 0.000 claims description 12
- 101000756632 Homo sapiens Actin, cytoplasmic 1 Proteins 0.000 claims description 12
- 230000008913 ectoderm development Effects 0.000 claims description 11
- 108010014064 CCCTC-Binding Factor Proteins 0.000 claims description 10
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims description 10
- 102100025744 Mothers against decapentaplegic homolog 1 Human genes 0.000 claims description 10
- 101700032040 SMAD1 Proteins 0.000 claims description 10
- 102100027671 Transcriptional repressor CTCF Human genes 0.000 claims description 10
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims description 10
- 101001056567 Homo sapiens Protein Jumonji Proteins 0.000 claims description 9
- 102100025733 Protein Jumonji Human genes 0.000 claims description 9
- 102100040685 14-3-3 protein zeta/delta Human genes 0.000 claims description 8
- 102100029511 26S proteasome regulatory subunit 6B Human genes 0.000 claims description 8
- 102100028104 39S ribosomal protein L19, mitochondrial Human genes 0.000 claims description 8
- 102100039882 40S ribosomal protein S17 Human genes 0.000 claims description 8
- 102100038237 60S ribosomal protein L30 Human genes 0.000 claims description 8
- 102100036126 60S ribosomal protein L37a Human genes 0.000 claims description 8
- 102100021921 ATP synthase subunit a Human genes 0.000 claims description 8
- 102100027314 Beta-2-microglobulin Human genes 0.000 claims description 8
- 102100026031 Beta-glucuronidase Human genes 0.000 claims description 8
- 108010016788 Cyclin-Dependent Kinase Inhibitor p21 Proteins 0.000 claims description 8
- 108010016777 Cyclin-Dependent Kinase Inhibitor p27 Proteins 0.000 claims description 8
- 102000000577 Cyclin-Dependent Kinase Inhibitor p27 Human genes 0.000 claims description 8
- 102100033270 Cyclin-dependent kinase inhibitor 1 Human genes 0.000 claims description 8
- 101000914063 Eucalyptus globulus Leafy/floricaula homolog FL1 Proteins 0.000 claims description 8
- 108010082945 Eukaryotic Initiation Factor-2B Proteins 0.000 claims description 8
- 102100027327 Eukaryotic translation initiation factor 2 subunit 2 Human genes 0.000 claims description 8
- 101000964898 Homo sapiens 14-3-3 protein zeta/delta Proteins 0.000 claims description 8
- 101001125524 Homo sapiens 26S proteasome regulatory subunit 6B Proteins 0.000 claims description 8
- 101001079803 Homo sapiens 39S ribosomal protein L19, mitochondrial Proteins 0.000 claims description 8
- 101000812077 Homo sapiens 40S ribosomal protein S17 Proteins 0.000 claims description 8
- 101001101319 Homo sapiens 60S ribosomal protein L30 Proteins 0.000 claims description 8
- 101001092424 Homo sapiens 60S ribosomal protein L37a Proteins 0.000 claims description 8
- 101000753741 Homo sapiens ATP synthase subunit a Proteins 0.000 claims description 8
- 101000937544 Homo sapiens Beta-2-microglobulin Proteins 0.000 claims description 8
- 101000933465 Homo sapiens Beta-glucuronidase Proteins 0.000 claims description 8
- 101000877395 Homo sapiens ETS-related transcription factor Elf-1 Proteins 0.000 claims description 8
- 101000896557 Homo sapiens Eukaryotic translation initiation factor 3 subunit B Proteins 0.000 claims description 8
- 101000988834 Homo sapiens Hypoxanthine-guanine phosphoribosyltransferase Proteins 0.000 claims description 8
- 101000608228 Homo sapiens NLR family pyrin domain-containing protein 2B Proteins 0.000 claims description 8
- 101001073193 Homo sapiens Pescadillo homolog Proteins 0.000 claims description 8
- 101000579123 Homo sapiens Phosphoglycerate kinase 1 Proteins 0.000 claims description 8
- 101000662049 Homo sapiens Polyubiquitin-C Proteins 0.000 claims description 8
- 101000947115 Homo sapiens Protein CASC3 Proteins 0.000 claims description 8
- 101000616974 Homo sapiens Pumilio homolog 1 Proteins 0.000 claims description 8
- 101000849714 Homo sapiens Ribonuclease P protein subunit p29 Proteins 0.000 claims description 8
- 101000881764 Homo sapiens Transcription elongation factor 1 homolog Proteins 0.000 claims description 8
- 101000835093 Homo sapiens Transferrin receptor protein 1 Proteins 0.000 claims description 8
- 101000823316 Homo sapiens Tyrosine-protein kinase ABL1 Proteins 0.000 claims description 8
- 102100039890 NLR family pyrin domain-containing protein 2B Human genes 0.000 claims description 8
- KJWZYMMLVHIVSU-IYCNHOCDSA-N PGK1 Chemical compound CCCCC[C@H](O)\C=C\[C@@H]1[C@@H](CCCCCCC(O)=O)C(=O)CC1=O KJWZYMMLVHIVSU-IYCNHOCDSA-N 0.000 claims description 8
- 102100035816 Pescadillo homolog Human genes 0.000 claims description 8
- 102100028251 Phosphoglycerate kinase 1 Human genes 0.000 claims description 8
- 102100037935 Polyubiquitin-C Human genes 0.000 claims description 8
- 102100035601 Protein CASC3 Human genes 0.000 claims description 8
- 102100021672 Pumilio homolog 1 Human genes 0.000 claims description 8
- 101710156592 Putative TATA-binding protein pB263R Proteins 0.000 claims description 8
- 102100040296 TATA-box-binding protein Human genes 0.000 claims description 8
- 101710145783 TATA-box-binding protein Proteins 0.000 claims description 8
- 102100037116 Transcription elongation factor 1 homolog Human genes 0.000 claims description 8
- 102100026144 Transferrin receptor protein 1 Human genes 0.000 claims description 8
- 102100022596 Tyrosine-protein kinase ABL1 Human genes 0.000 claims description 8
- AQQSXKSWTNWXKR-UHFFFAOYSA-N 2-(2-phenylphenanthro[9,10-d]imidazol-3-yl)acetic acid Chemical compound C1(=CC=CC=C1)C1=NC2=C(N1CC(=O)O)C1=CC=CC=C1C=1C=CC=CC=12 AQQSXKSWTNWXKR-UHFFFAOYSA-N 0.000 claims description 7
- 102100040881 60S acidic ribosomal protein P0 Human genes 0.000 claims description 7
- 102100021429 DNA-directed RNA polymerase II subunit RPB1 Human genes 0.000 claims description 7
- 101000673456 Homo sapiens 60S acidic ribosomal protein P0 Proteins 0.000 claims description 7
- 101001106401 Homo sapiens DNA-directed RNA polymerase II subunit RPB1 Proteins 0.000 claims description 7
- 101000599449 Homo sapiens Importin-8 Proteins 0.000 claims description 7
- 101001067833 Homo sapiens Peptidyl-prolyl cis-trans isomerase A Proteins 0.000 claims description 7
- 102100037966 Importin-8 Human genes 0.000 claims description 7
- 102100034539 Peptidyl-prolyl cis-trans isomerase A Human genes 0.000 claims description 7
- 102100034391 Porphobilinogen deaminase Human genes 0.000 claims description 7
- 101710189720 Porphobilinogen deaminase Proteins 0.000 claims description 7
- 101710170827 Porphobilinogen deaminase, chloroplastic Proteins 0.000 claims description 7
- 101710100896 Probable porphobilinogen deaminase Proteins 0.000 claims description 7
- CXVGEDCSTKKODG-UHFFFAOYSA-N sulisobenzone Chemical compound C1=C(S(O)(=O)=O)C(OC)=CC(O)=C1C(=O)C1=CC=CC=C1 CXVGEDCSTKKODG-UHFFFAOYSA-N 0.000 claims description 7
- 239000007850 fluorescent dye Substances 0.000 claims description 5
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims description 4
- 239000002751 oligonucleotide probe Substances 0.000 claims description 4
- 239000000975 dye Substances 0.000 claims description 2
- 102100021699 Eukaryotic translation initiation factor 3 subunit B Human genes 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 450
- 210000002242 embryoid body Anatomy 0.000 description 80
- 239000002609 medium Substances 0.000 description 59
- 229920002477 rna polymer Polymers 0.000 description 40
- 238000003757 reverse transcription PCR Methods 0.000 description 37
- 238000004458 analytical method Methods 0.000 description 35
- 238000011529 RT qPCR Methods 0.000 description 34
- 238000002493 microarray Methods 0.000 description 34
- 238000003491 array Methods 0.000 description 33
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 33
- 238000003752 polymerase chain reaction Methods 0.000 description 33
- 238000003860 storage Methods 0.000 description 33
- 238000012360 testing method Methods 0.000 description 32
- 201000010099 disease Diseases 0.000 description 31
- 102000004169 proteins and genes Human genes 0.000 description 30
- 238000009396 hybridization Methods 0.000 description 27
- 230000001965 increasing effect Effects 0.000 description 27
- 108020004414 DNA Proteins 0.000 description 24
- 238000000692 Student's t-test Methods 0.000 description 24
- 238000012353 t test Methods 0.000 description 24
- 210000001519 tissue Anatomy 0.000 description 23
- 230000008672 reprogramming Effects 0.000 description 22
- 238000001514 detection method Methods 0.000 description 21
- 238000003753 real-time PCR Methods 0.000 description 21
- 238000010606 normalization Methods 0.000 description 20
- 230000000295 complement effect Effects 0.000 description 19
- 239000003550 marker Substances 0.000 description 19
- 239000003814 drug Substances 0.000 description 18
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 18
- 108091028043 Nucleic acid sequence Proteins 0.000 description 17
- 238000010804 cDNA synthesis Methods 0.000 description 17
- 210000001671 embryonic stem cell Anatomy 0.000 description 17
- 230000006870 function Effects 0.000 description 17
- 239000000463 material Substances 0.000 description 16
- 210000001082 somatic cell Anatomy 0.000 description 16
- 238000004422 calculation algorithm Methods 0.000 description 15
- 230000008859 change Effects 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 15
- 238000011160 research Methods 0.000 description 15
- 150000001875 compounds Chemical class 0.000 description 14
- 210000004602 germ cell Anatomy 0.000 description 14
- 210000001654 germ layer Anatomy 0.000 description 14
- 230000008093 supporting effect Effects 0.000 description 14
- 238000010195 expression analysis Methods 0.000 description 13
- 230000001225 therapeutic effect Effects 0.000 description 13
- 238000011282 treatment Methods 0.000 description 13
- 239000000090 biomarker Substances 0.000 description 12
- 230000024245 cell differentiation Effects 0.000 description 12
- 230000000875 corresponding effect Effects 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 12
- 238000010200 validation analysis Methods 0.000 description 12
- 238000007397 LAMP assay Methods 0.000 description 11
- 230000001537 neural effect Effects 0.000 description 11
- 229940079593 drug Drugs 0.000 description 10
- 230000037361 pathway Effects 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 238000012545 processing Methods 0.000 description 10
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 210000004504 adult stem cell Anatomy 0.000 description 9
- 238000012512 characterization method Methods 0.000 description 9
- 238000012258 culturing Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 230000002829 reductive effect Effects 0.000 description 9
- 230000001105 regulatory effect Effects 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 8
- 102100024014 Nestin Human genes 0.000 description 8
- 102100021380 Transcription factor GATA-4 Human genes 0.000 description 8
- 238000004590 computer program Methods 0.000 description 8
- 239000011521 glass Substances 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 8
- 230000003394 haemopoietic effect Effects 0.000 description 8
- 239000010410 layer Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000001404 mediated effect Effects 0.000 description 8
- 230000003287 optical effect Effects 0.000 description 8
- 102000004196 processed proteins & peptides Human genes 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 102100032912 CD44 antigen Human genes 0.000 description 7
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 7
- 102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 7
- 230000007423 decrease Effects 0.000 description 7
- 238000006073 displacement reaction Methods 0.000 description 7
- 238000007877 drug screening Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000001727 in vivo Methods 0.000 description 7
- 230000001172 regenerating effect Effects 0.000 description 7
- 230000010076 replication Effects 0.000 description 7
- 102100036364 Cadherin-2 Human genes 0.000 description 6
- 101000714537 Homo sapiens Cadherin-2 Proteins 0.000 description 6
- 101000994365 Homo sapiens Integrin alpha-6 Proteins 0.000 description 6
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 6
- 101001111320 Homo sapiens Nestin Proteins 0.000 description 6
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 6
- 102100032816 Integrin alpha-6 Human genes 0.000 description 6
- 102100025304 Integrin beta-1 Human genes 0.000 description 6
- 108010032788 PAX6 Transcription Factor Proteins 0.000 description 6
- 102100037506 Paired box protein Pax-6 Human genes 0.000 description 6
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 6
- 238000013500 data storage Methods 0.000 description 6
- 230000009871 nonspecific binding Effects 0.000 description 6
- 238000003908 quality control method Methods 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 238000010839 reverse transcription Methods 0.000 description 6
- 238000005096 rolling process Methods 0.000 description 6
- 230000007067 DNA methylation Effects 0.000 description 5
- 102100031150 Growth arrest and DNA damage-inducible protein GADD45 alpha Human genes 0.000 description 5
- 102100027694 Homeobox protein engrailed-1 Human genes 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- 101001066158 Homo sapiens Growth arrest and DNA damage-inducible protein GADD45 alpha Proteins 0.000 description 5
- 101001081126 Homo sapiens Homeobox protein engrailed-1 Proteins 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 230000006399 behavior Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000027455 binding Effects 0.000 description 5
- 238000003766 bioinformatics method Methods 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000004891 communication Methods 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 5
- 210000003494 hepatocyte Anatomy 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 210000001988 somatic stem cell Anatomy 0.000 description 5
- 241000894007 species Species 0.000 description 5
- PJOHVEQSYPOERL-SHEAVXILSA-N (e)-n-[(4r,4as,7ar,12br)-3-(cyclopropylmethyl)-9-hydroxy-7-oxo-2,4,5,6,7a,13-hexahydro-1h-4,12-methanobenzofuro[3,2-e]isoquinoline-4a-yl]-3-(4-methylphenyl)prop-2-enamide Chemical compound C1=CC(C)=CC=C1\C=C\C(=O)N[C@]1(CCC(=O)[C@@H]2O3)[C@H]4CC5=CC=C(O)C3=C5[C@]12CCN4CC1CC1 PJOHVEQSYPOERL-SHEAVXILSA-N 0.000 description 4
- 101150037123 APOE gene Proteins 0.000 description 4
- 102100035248 Alpha-(1,3)-fucosyltransferase 4 Human genes 0.000 description 4
- 102100029470 Apolipoprotein E Human genes 0.000 description 4
- 102100022595 Broad substrate specificity ATP-binding cassette transporter ABCG2 Human genes 0.000 description 4
- 108010083123 CDX2 Transcription Factor Proteins 0.000 description 4
- 102100029761 Cadherin-5 Human genes 0.000 description 4
- 102100023126 Cell surface glycoprotein MUC18 Human genes 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 108700039887 Essential Genes Proteins 0.000 description 4
- 102100035961 Hematopoietically-expressed homeobox protein HHEX Human genes 0.000 description 4
- 102100038885 Histone acetyltransferase p300 Human genes 0.000 description 4
- 102100031671 Homeobox protein CDX-2 Human genes 0.000 description 4
- 101001022185 Homo sapiens Alpha-(1,3)-fucosyltransferase 4 Proteins 0.000 description 4
- 101000794587 Homo sapiens Cadherin-5 Proteins 0.000 description 4
- 101000623903 Homo sapiens Cell surface glycoprotein MUC18 Proteins 0.000 description 4
- 101001021503 Homo sapiens Hematopoietically-expressed homeobox protein HHEX Proteins 0.000 description 4
- 101000882390 Homo sapiens Histone acetyltransferase p300 Proteins 0.000 description 4
- 101000994375 Homo sapiens Integrin alpha-4 Proteins 0.000 description 4
- 101000599852 Homo sapiens Intercellular adhesion molecule 1 Proteins 0.000 description 4
- 101000972291 Homo sapiens Lymphoid enhancer-binding factor 1 Proteins 0.000 description 4
- 101000581981 Homo sapiens Neural cell adhesion molecule 1 Proteins 0.000 description 4
- 101000603702 Homo sapiens Neurogenin-3 Proteins 0.000 description 4
- 101000604123 Homo sapiens Noggin Proteins 0.000 description 4
- 101000695522 Homo sapiens Synaptophysin Proteins 0.000 description 4
- 101000835745 Homo sapiens Teratocarcinoma-derived growth factor 1 Proteins 0.000 description 4
- 101000819111 Homo sapiens Trans-acting T-cell-specific transcription factor GATA-3 Proteins 0.000 description 4
- 102100024392 Insulin gene enhancer protein ISL-1 Human genes 0.000 description 4
- 102100032818 Integrin alpha-4 Human genes 0.000 description 4
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 description 4
- 102100022699 Lymphoid enhancer-binding factor 1 Human genes 0.000 description 4
- 108010090306 Member 2 Subfamily G ATP Binding Cassette Transporter Proteins 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 108010032605 Nerve Growth Factor Receptors Proteins 0.000 description 4
- 102100027347 Neural cell adhesion molecule 1 Human genes 0.000 description 4
- 102100038553 Neurogenin-3 Human genes 0.000 description 4
- 102100038454 Noggin Human genes 0.000 description 4
- 102000001759 Notch1 Receptor Human genes 0.000 description 4
- 108010029755 Notch1 Receptor Proteins 0.000 description 4
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 description 4
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 description 4
- 239000013614 RNA sample Substances 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 4
- 102100028706 Synaptophysin Human genes 0.000 description 4
- 102100026404 Teratocarcinoma-derived growth factor 1 Human genes 0.000 description 4
- 206010043276 Teratoma Diseases 0.000 description 4
- 102100021386 Trans-acting T-cell-specific transcription factor GATA-3 Human genes 0.000 description 4
- 102100033725 Tumor necrosis factor receptor superfamily member 16 Human genes 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000000747 cardiac effect Effects 0.000 description 4
- 210000004413 cardiac myocyte Anatomy 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 238000007726 management method Methods 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 150000002739 metals Chemical class 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 239000005022 packaging material Substances 0.000 description 4
- 230000036961 partial effect Effects 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 229910052710 silicon Inorganic materials 0.000 description 4
- 239000010703 silicon Substances 0.000 description 4
- 238000007619 statistical method Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000002723 toxicity assay Methods 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 238000002054 transplantation Methods 0.000 description 4
- 230000032258 transport Effects 0.000 description 4
- 238000012795 verification Methods 0.000 description 4
- 108020005544 Antisense RNA Proteins 0.000 description 3
- 241000282472 Canis lupus familiaris Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000282326 Felis catus Species 0.000 description 3
- 102100022123 Hepatocyte nuclear factor 1-beta Human genes 0.000 description 3
- 101001045758 Homo sapiens Hepatocyte nuclear factor 1-beta Proteins 0.000 description 3
- 101000979001 Homo sapiens Methionine aminopeptidase 2 Proteins 0.000 description 3
- 101000969087 Homo sapiens Microtubule-associated protein 2 Proteins 0.000 description 3
- 101000613490 Homo sapiens Paired box protein Pax-3 Proteins 0.000 description 3
- 101001116302 Homo sapiens Platelet endothelial cell adhesion molecule Proteins 0.000 description 3
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 description 3
- 101000633054 Homo sapiens Zinc finger protein SNAI2 Proteins 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- 102100023174 Methionine aminopeptidase 2 Human genes 0.000 description 3
- 108700011259 MicroRNAs Proteins 0.000 description 3
- 238000000636 Northern blotting Methods 0.000 description 3
- 102100040891 Paired box protein Pax-3 Human genes 0.000 description 3
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 description 3
- 241000288906 Primates Species 0.000 description 3
- 108091034057 RNA (poly(A)) Proteins 0.000 description 3
- 238000002123 RNA extraction Methods 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- 102100024270 Transcription factor SOX-2 Human genes 0.000 description 3
- 108020004566 Transfer RNA Proteins 0.000 description 3
- 102100029570 Zinc finger protein SNAI2 Human genes 0.000 description 3
- 206010002026 amyotrophic lateral sclerosis Diseases 0.000 description 3
- 210000004102 animal cell Anatomy 0.000 description 3
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 210000000845 cartilage Anatomy 0.000 description 3
- 239000006143 cell culture medium Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000007876 drug discovery Methods 0.000 description 3
- 238000007878 drug screening assay Methods 0.000 description 3
- 238000010201 enrichment analysis Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 230000001973 epigenetic effect Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000010199 gene set enrichment analysis Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000012203 high throughput assay Methods 0.000 description 3
- 238000013537 high throughput screening Methods 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 239000002679 microRNA Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 210000000663 muscle cell Anatomy 0.000 description 3
- 210000001178 neural stem cell Anatomy 0.000 description 3
- 208000015122 neurodegenerative disease Diseases 0.000 description 3
- 238000007899 nucleic acid hybridization Methods 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 101150087690 ACTB gene Proteins 0.000 description 2
- 102100022089 Acyl-[acyl-carrier-protein] hydrolase Human genes 0.000 description 2
- 102100031786 Adiponectin Human genes 0.000 description 2
- 102100022749 Aminopeptidase N Human genes 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 101000964894 Bos taurus 14-3-3 protein zeta/delta Proteins 0.000 description 2
- 108010017009 CD11b Antigen Proteins 0.000 description 2
- 108010049990 CD13 Antigens Proteins 0.000 description 2
- 102000049320 CD36 Human genes 0.000 description 2
- 108010045374 CD36 Antigens Proteins 0.000 description 2
- 108010062802 CD66 antigens Proteins 0.000 description 2
- 102100025805 Cadherin-1 Human genes 0.000 description 2
- 241000282421 Canidae Species 0.000 description 2
- 102100024533 Carcinoembryonic antigen-related cell adhesion molecule 1 Human genes 0.000 description 2
- 102100028914 Catenin beta-1 Human genes 0.000 description 2
- 108091007854 Cdh1/Fizzy-related Proteins 0.000 description 2
- 102100038504 Cellular retinoic acid-binding protein 2 Human genes 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 208000015943 Coeliac disease Diseases 0.000 description 2
- 101100481404 Danio rerio tie1 gene Proteins 0.000 description 2
- 101100107081 Danio rerio zbtb16a gene Proteins 0.000 description 2
- 102100036462 Delta-like protein 1 Human genes 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102100031785 Endothelial transcription factor GATA-2 Human genes 0.000 description 2
- 101000823089 Equus caballus Alpha-1-antiproteinase 1 Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 102100023600 Fibroblast growth factor receptor 2 Human genes 0.000 description 2
- 101710182389 Fibroblast growth factor receptor 2 Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 description 2
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 2
- 102000058058 Glucose Transporter Type 2 Human genes 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 206010019280 Heart failures Diseases 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 102100022057 Hepatocyte nuclear factor 1-alpha Human genes 0.000 description 2
- 102100027875 Homeobox protein Nkx-2.5 Human genes 0.000 description 2
- 102100030634 Homeobox protein OTX2 Human genes 0.000 description 2
- 101000824278 Homo sapiens Acyl-[acyl-carrier-protein] hydrolase Proteins 0.000 description 2
- 101000775469 Homo sapiens Adiponectin Proteins 0.000 description 2
- 101000916173 Homo sapiens Catenin beta-1 Proteins 0.000 description 2
- 101001099851 Homo sapiens Cellular retinoic acid-binding protein 2 Proteins 0.000 description 2
- 101000928537 Homo sapiens Delta-like protein 1 Proteins 0.000 description 2
- 101001066265 Homo sapiens Endothelial transcription factor GATA-2 Proteins 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101001045751 Homo sapiens Hepatocyte nuclear factor 1-alpha Proteins 0.000 description 2
- 101000632197 Homo sapiens Homeobox protein Nkx-2.5 Proteins 0.000 description 2
- 101000584400 Homo sapiens Homeobox protein OTX2 Proteins 0.000 description 2
- 101001053263 Homo sapiens Insulin gene enhancer protein ISL-1 Proteins 0.000 description 2
- 101001046683 Homo sapiens Integrin alpha-L Proteins 0.000 description 2
- 101001046677 Homo sapiens Integrin alpha-V Proteins 0.000 description 2
- 101001046668 Homo sapiens Integrin alpha-X Proteins 0.000 description 2
- 101001015004 Homo sapiens Integrin beta-3 Proteins 0.000 description 2
- 101001020548 Homo sapiens LIM/homeobox protein Lhx1 Proteins 0.000 description 2
- 101000976913 Homo sapiens Lens fiber major intrinsic protein Proteins 0.000 description 2
- 101000891579 Homo sapiens Microtubule-associated protein tau Proteins 0.000 description 2
- 101000576323 Homo sapiens Motor neuron and pancreas homeobox protein 1 Proteins 0.000 description 2
- 101001023043 Homo sapiens Myoblast determination protein 1 Proteins 0.000 description 2
- 101000589002 Homo sapiens Myogenin Proteins 0.000 description 2
- 101000601661 Homo sapiens Paired box protein Pax-7 Proteins 0.000 description 2
- 101001126417 Homo sapiens Platelet-derived growth factor receptor alpha Proteins 0.000 description 2
- 101000874179 Homo sapiens Syndecan-1 Proteins 0.000 description 2
- 101000716102 Homo sapiens T-cell surface glycoprotein CD4 Proteins 0.000 description 2
- 101000819088 Homo sapiens Transcription factor GATA-6 Proteins 0.000 description 2
- 101000651211 Homo sapiens Transcription factor PU.1 Proteins 0.000 description 2
- 101000664703 Homo sapiens Transcription factor SOX-10 Proteins 0.000 description 2
- 101000711846 Homo sapiens Transcription factor SOX-9 Proteins 0.000 description 2
- 101000801228 Homo sapiens Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 description 2
- 101000611023 Homo sapiens Tumor necrosis factor receptor superfamily member 6 Proteins 0.000 description 2
- 101100377226 Homo sapiens ZBTB16 gene Proteins 0.000 description 2
- 102100027004 Inhibin beta A chain Human genes 0.000 description 2
- 102100022339 Integrin alpha-L Human genes 0.000 description 2
- 102100022338 Integrin alpha-M Human genes 0.000 description 2
- 102100022337 Integrin alpha-V Human genes 0.000 description 2
- 102100022297 Integrin alpha-X Human genes 0.000 description 2
- 102100032999 Integrin beta-3 Human genes 0.000 description 2
- 102100036133 LIM/homeobox protein Lhx1 Human genes 0.000 description 2
- 208000015439 Lysosomal storage disease Diseases 0.000 description 2
- 241000282560 Macaca mulatta Species 0.000 description 2
- 208000024556 Mendelian disease Diseases 0.000 description 2
- 102100040243 Microtubule-associated protein tau Human genes 0.000 description 2
- 102100025170 Motor neuron and pancreas homeobox protein 1 Human genes 0.000 description 2
- 101100022875 Mus musculus Meox1 gene Proteins 0.000 description 2
- 101100310648 Mus musculus Sox17 gene Proteins 0.000 description 2
- 101100043062 Mus musculus Sox7 gene Proteins 0.000 description 2
- 101100481406 Mus musculus Tie1 gene Proteins 0.000 description 2
- 208000029549 Muscle injury Diseases 0.000 description 2
- 102100035077 Myoblast determination protein 1 Human genes 0.000 description 2
- 102100032970 Myogenin Human genes 0.000 description 2
- 102000003729 Neprilysin Human genes 0.000 description 2
- 108090000028 Neprilysin Proteins 0.000 description 2
- 108010088225 Nestin Proteins 0.000 description 2
- 102100023057 Neurofilament light polypeptide Human genes 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 description 2
- 102100037503 Paired box protein Pax-7 Human genes 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 102100030485 Platelet-derived growth factor receptor alpha Human genes 0.000 description 2
- 108700003766 Promyelocytic Leukemia Zinc Finger Proteins 0.000 description 2
- 238000003559 RNA-seq method Methods 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- 108091006299 SLC2A2 Proteins 0.000 description 2
- 108010017324 STAT3 Transcription Factor Proteins 0.000 description 2
- 108010042291 Serum Response Factor Proteins 0.000 description 2
- 102100022056 Serum response factor Human genes 0.000 description 2
- 102100024040 Signal transducer and activator of transcription 3 Human genes 0.000 description 2
- 102100035721 Syndecan-1 Human genes 0.000 description 2
- 102100036011 T-cell surface glycoprotein CD4 Human genes 0.000 description 2
- 102100021382 Transcription factor GATA-6 Human genes 0.000 description 2
- 102100027654 Transcription factor PU.1 Human genes 0.000 description 2
- 102100038808 Transcription factor SOX-10 Human genes 0.000 description 2
- 102100034204 Transcription factor SOX-9 Human genes 0.000 description 2
- 102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 description 2
- 108010083162 Twist-Related Protein 1 Proteins 0.000 description 2
- 102100030398 Twist-related protein 1 Human genes 0.000 description 2
- 108010053099 Vascular Endothelial Growth Factor Receptor-2 Proteins 0.000 description 2
- 102100033177 Vascular endothelial growth factor receptor 2 Human genes 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 101001062354 Xenopus tropicalis Forkhead box protein A2 Proteins 0.000 description 2
- 102100040314 Zinc finger and BTB domain-containing protein 16 Human genes 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 239000003184 complementary RNA Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000005547 deoxyribonucleotide Substances 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 230000002526 effect on cardiovascular system Effects 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 101150003286 gata4 gene Proteins 0.000 description 2
- 238000003633 gene expression assay Methods 0.000 description 2
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 210000002865 immune cell Anatomy 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000007901 in situ hybridization Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 108010019691 inhibin beta A subunit Proteins 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 108010090448 insulin gene enhancer binding protein Isl-1 Proteins 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000007477 logistic regression Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 238000007620 mathematical function Methods 0.000 description 2
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000010607 microarray normalization Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 210000002161 motor neuron Anatomy 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000001665 muscle stem cell Anatomy 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 210000005055 nestin Anatomy 0.000 description 2
- 108010090677 neurofilament protein L Proteins 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 230000007823 neuropathy Effects 0.000 description 2
- 201000001119 neuropathy Diseases 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000002966 oligonucleotide array Methods 0.000 description 2
- 208000033808 peripheral neuropathy Diseases 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000004043 responsiveness Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- 108020004418 ribosomal RNA Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 210000003491 skin Anatomy 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 238000009168 stem cell therapy Methods 0.000 description 2
- 238000009580 stem-cell therapy Methods 0.000 description 2
- 238000012706 support-vector machine Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- 238000007910 systemic administration Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 206010000830 Acute leukaemia Diseases 0.000 description 1
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 1
- 101000993093 Arabidopsis thaliana Heat stress transcription factor B-2a Proteins 0.000 description 1
- 241000282672 Ateles sp. Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 102100031680 Beta-catenin-interacting protein 1 Human genes 0.000 description 1
- 241000157302 Bison bison athabascae Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 241000271571 Dromaius novaehollandiae Species 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 101000885147 Enterococcus avium D-arabitol-phosphate dehydrogenase Proteins 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 240000003537 Ficus benghalensis Species 0.000 description 1
- 238000000729 Fisher's exact test Methods 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 description 1
- 206010071602 Genetic polymorphism Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102100032606 Heat shock factor protein 1 Human genes 0.000 description 1
- 101710190344 Heat shock factor protein 1 Proteins 0.000 description 1
- 101710113864 Heat shock protein 90 Proteins 0.000 description 1
- 102100034051 Heat shock protein HSP 90-alpha Human genes 0.000 description 1
- 102100022054 Hepatocyte nuclear factor 4-alpha Human genes 0.000 description 1
- 101150029234 Hes5 gene Proteins 0.000 description 1
- 101000993469 Homo sapiens Beta-catenin-interacting protein 1 Proteins 0.000 description 1
- 101001045740 Homo sapiens Hepatocyte nuclear factor 4-alpha Proteins 0.000 description 1
- 101000666775 Homo sapiens T-box transcription factor TBX3 Proteins 0.000 description 1
- 101000843556 Homo sapiens Transcription factor HES-1 Proteins 0.000 description 1
- 101000652332 Homo sapiens Transcription factor SOX-1 Proteins 0.000 description 1
- 101710123134 Ice-binding protein Proteins 0.000 description 1
- 101710082837 Ice-structuring protein Proteins 0.000 description 1
- 102100023915 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 description 1
- 241000282553 Macaca Species 0.000 description 1
- 241000282567 Macaca fascicularis Species 0.000 description 1
- 241001000161 Mago Species 0.000 description 1
- 241000283923 Marmota monax Species 0.000 description 1
- 241000282341 Mustela putorius furo Species 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
- 208000034176 Neoplasms, Germ Cell and Embryonal Diseases 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 241000282577 Pan troglodytes Species 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 108091036407 Polyadenylation Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 101100016889 Rattus norvegicus Hes2 gene Proteins 0.000 description 1
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 208000013007 Rodent disease Diseases 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 241000271567 Struthioniformes Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 102100038409 T-box transcription factor TBX3 Human genes 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- 102100030798 Transcription factor HES-1 Human genes 0.000 description 1
- 102100030248 Transcription factor SOX-1 Human genes 0.000 description 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
- 241000269386 Triturus Species 0.000 description 1
- 101710107540 Type-2 ice-structuring protein Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000036676 acute undifferentiated leukemia Diseases 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000007698 birth defect Effects 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 210000004703 blastocyst inner cell mass Anatomy 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 210000000133 brain stem Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 230000001269 cardiogenic effect Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 230000002648 chondrogenic effect Effects 0.000 description 1
- 230000010428 chromatin condensation Effects 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000002790 cross-validation Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000009274 differential gene expression Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 208000037765 diseases and disorders Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000036267 drug metabolism Effects 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 201000002246 embryonal cancer Diseases 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000004039 endoderm cell Anatomy 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000004076 epigenetic alteration Effects 0.000 description 1
- 230000008995 epigenetic change Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000002304 esc Anatomy 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000004547 gene signature Effects 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 230000037442 genomic alteration Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 210000005003 heart tissue Anatomy 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007641 inkjet printing Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 210000004966 intestinal stem cell Anatomy 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000001361 intraarterial administration Methods 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 238000007913 intrathecal administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000007914 intraventricular administration Methods 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004989 laser desorption mass spectroscopy Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000003738 lymphoid progenitor cell Anatomy 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 238000010841 mRNA extraction Methods 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 241001515942 marmosets Species 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 230000007431 mesendoderm development Effects 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000010208 microarray analysis Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 210000003098 myoblast Anatomy 0.000 description 1
- 210000000441 neoplastic stem cell Anatomy 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 229920006284 nylon film Polymers 0.000 description 1
- 230000005868 ontogenesis Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000004963 pathophysiological condition Effects 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000001428 peripheral nervous system Anatomy 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 230000002974 pharmacogenomic effect Effects 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 238000011158 quantitative evaluation Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000009256 replacement therapy Methods 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 238000011808 rodent model Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 238000013515 script Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000010374 somatic cell nuclear transfer Methods 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 210000002536 stromal cell Anatomy 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000000756 surface-enhanced laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 101150065190 term gene Proteins 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 238000011200 topical administration Methods 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 210000002444 unipotent stem cell Anatomy 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 210000001635 urinary tract Anatomy 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 230000001755 vocal effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361833092P | 2013-06-10 | 2013-06-10 | |
| US61/833,092 | 2013-06-10 | ||
| PCT/US2014/041513 WO2014200905A2 (en) | 2013-06-10 | 2014-06-09 | Early developmental genomic assay for characterizing pluripotent stem cell utility and safety |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016532435A JP2016532435A (ja) | 2016-10-20 |
| JP2016532435A5 JP2016532435A5 (enExample) | 2017-07-20 |
| JP6697380B2 true JP6697380B2 (ja) | 2020-05-20 |
Family
ID=52022907
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016519572A Active JP6697380B2 (ja) | 2013-06-10 | 2014-06-09 | 多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US11085067B2 (enExample) |
| EP (1) | EP3008229B1 (enExample) |
| JP (1) | JP6697380B2 (enExample) |
| WO (1) | WO2014200905A2 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015193951A1 (ja) | 2014-06-16 | 2015-12-23 | 株式会社ニコン | 観察装置、観察方法、観察システム、そのプログラム、および細胞の製造方法 |
| CN117201777A (zh) | 2016-09-13 | 2023-12-08 | 韩国电子通信研究院 | 视频编码/解码方法和装置以及存储比特流的记录介质 |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| US11603563B2 (en) * | 2017-06-10 | 2023-03-14 | Shimadzu Corporation | Method of predicting differentiation potential of iPS cells into cartilage cells based on gene expression profiles |
| AU2018324301A1 (en) * | 2017-09-01 | 2020-04-16 | Orig3N, Inc. | I-PSC derived secretome compositions, and related systems and methods |
| WO2019078342A1 (ja) * | 2017-10-20 | 2019-04-25 | 国立大学法人大阪大学 | 心筋細胞への分化指向性を有する多能性幹細胞を選抜するための方法 |
| CN113646424A (zh) * | 2019-03-29 | 2021-11-12 | 富士胶片株式会社 | 具有分化成特定细胞的能力的多能干细胞的制造方法及其应用 |
| WO2021095798A1 (ja) * | 2019-11-15 | 2021-05-20 | 公立大学法人横浜市立大学 | 未分化マーカー遺伝子高感度検出法 |
| JPWO2021145402A1 (enExample) * | 2020-01-16 | 2021-07-22 | ||
| US10968493B1 (en) * | 2020-03-12 | 2021-04-06 | New England Biolabs, Inc. | Rapid diagnostic test using colorimetric LAMP |
| JPWO2021256078A1 (enExample) * | 2020-06-19 | 2021-12-23 | ||
| EP3940704A1 (en) * | 2020-07-14 | 2022-01-19 | Koninklijke Philips N.V. | Method for determining the differentiation state of a stem cell |
| CN112575010B (zh) * | 2020-12-14 | 2022-11-08 | 云南农业大学 | 山药不同组织荧光定量的内参基因及其引物与应用 |
| CN119137667A (zh) * | 2022-03-25 | 2024-12-13 | 宾州研究基金会 | 针对基因组甲基化数据的基因组词框架分析 |
| WO2023201361A1 (en) * | 2022-04-15 | 2023-10-19 | Aspen Neuroscience, Inc. | Methods of classifying the differentiation state of cells and related compositions of differentiated cells |
Family Cites Families (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US548257A (en) | 1895-10-22 | Hay rake and loader | ||
| US4963489A (en) | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| US6270961B1 (en) | 1987-04-01 | 2001-08-07 | Hyseq, Inc. | Methods and apparatus for DNA sequencing and DNA identification |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| CA2040503A1 (en) | 1990-04-25 | 1991-10-26 | James E. Stefano | Selective amplification system using q beta replicase |
| DE69132905T2 (de) | 1990-12-06 | 2002-08-01 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Detektion von Nukleinsäuresequenzen |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| AU4543193A (en) | 1992-06-22 | 1994-01-24 | Henry E. Young | Scar inhibitory factor and use thereof |
| US5672346A (en) | 1992-07-27 | 1997-09-30 | Indiana University Foundation | Human stem cell compositions and methods |
| CA2146747C (en) | 1992-10-09 | 2006-12-19 | Brian A. Naughton | Liver reserve cells |
| US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| US6136540A (en) | 1994-10-03 | 2000-10-24 | Ikonisys Inc. | Automated fluorescence in situ hybridization detection of genetic abnormalities |
| US5952172A (en) | 1993-12-10 | 1999-09-14 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US6379897B1 (en) | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
| US5622826A (en) | 1994-12-22 | 1997-04-22 | Houston Advanced Research Center | Method for immobilization of molecules on platinum solid support surfaces |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| US5968740A (en) | 1995-07-24 | 1999-10-19 | Affymetrix, Inc. | Method of Identifying a Base in a Nucleic Acid |
| US5569588A (en) | 1995-08-09 | 1996-10-29 | The Regents Of The University Of California | Methods for drug screening |
| US5994619A (en) | 1996-04-01 | 1999-11-30 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Production of chimeric bovine or porcine animals using cultured inner cell mass cells |
| EP0990142A4 (en) | 1996-12-31 | 2000-09-27 | Genometrix Genomics Inc | MULTIPLEXED MOLECULAR ANALYSIS METHOD AND DEVICE |
| US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US6465611B1 (en) | 1997-02-25 | 2002-10-15 | Corixa Corporation | Compounds for immunotherapy of prostate cancer and methods for their use |
| US6642433B1 (en) | 1997-05-15 | 2003-11-04 | Trillium Therapeutics Inc. | Fgl-2 knockout mice |
| CA2316912C (en) | 1998-01-12 | 2009-09-15 | Massachusetts Institute Of Technology | Method and apparatus for performing microassays |
| US6893877B2 (en) | 1998-01-12 | 2005-05-17 | Massachusetts Institute Of Technology | Methods for screening substances in a microwell array |
| US6235473B1 (en) | 1998-07-02 | 2001-05-22 | Orchid Biosciences, Inc. | Gene pen devices for array printing |
| WO2001055454A1 (en) | 2000-01-28 | 2001-08-02 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| US7332271B2 (en) | 2000-02-18 | 2008-02-19 | Board Of Trustees Of The Leland Stanford Junior University | Apparatus and methods for parallel processing of micro-volume liquid reactions |
| KR20020097181A (ko) | 2000-02-22 | 2002-12-31 | 제노스펙트라 인코포레이티드 | 마이크로어레이 제작 기술 및 장치 |
| WO2001075162A2 (en) | 2000-03-31 | 2001-10-11 | University Of Louisville Research Foundation, Inc. | Microarrays to screen regulatory genes |
| AU8617301A (en) | 2000-08-01 | 2002-02-13 | Yissum Res Dev Co | Directed differentiation of embryonic cells |
| US6902702B1 (en) | 2000-08-16 | 2005-06-07 | University Health Network | Devices and methods for producing microarrays of biological samples |
| AU2001286171B2 (en) | 2001-05-25 | 2008-01-10 | Serono Genetics Institute S.A. | Human CDNAs and proteins and uses thereof |
| WO2003043402A2 (en) | 2001-10-19 | 2003-05-30 | Proligo Llc | Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes |
| US20030113910A1 (en) | 2001-12-18 | 2003-06-19 | Mike Levanduski | Pluripotent stem cells derived without the use of embryos or fetal tissue |
| US20030215858A1 (en) | 2002-04-08 | 2003-11-20 | Baylor College Of Medicine | Enhanced gene expression system |
| US20030194711A1 (en) | 2002-04-10 | 2003-10-16 | Matthew Zapala | System and method for analyzing gene expression data |
| US20040162679A1 (en) | 2002-05-03 | 2004-08-19 | Linheng Li | Method for predicting gene potential and cell commitment |
| WO2004009758A2 (en) | 2002-07-23 | 2004-01-29 | Nanodiagnostics, Inc. | Embryonic stem cell markers and uses thereof |
| US7153650B2 (en) | 2003-03-13 | 2006-12-26 | Geron Corporation | Marker system for preparing and characterizing high-quality human embryonic stem cells |
| US20060177825A1 (en) | 2003-04-29 | 2006-08-10 | Mcdonald John F | Global analysis of transposable elements as molecular markers of the developmental potential of stem cells |
| GB0413005D0 (en) | 2004-06-11 | 2004-07-14 | Coletica | Ligand |
| GB0504427D0 (en) * | 2005-03-03 | 2005-04-06 | Roslin Inst Edinburgh | Method for differentiation of stem cells |
| EP1948822B1 (en) | 2005-10-07 | 2011-07-13 | Johnson & Johnson Research Pty Limited | Multicomponent nucleic acid enzymes and methods for their use |
| CA2626152A1 (en) | 2005-10-24 | 2007-05-03 | Agency For Science, Technology And Research | Methods of specifying mesodermal, endodermal and mesoendodermal cell fates |
| US20090227032A1 (en) | 2005-12-13 | 2009-09-10 | Kyoto University | Nuclear reprogramming factor and induced pluripotent stem cells |
| KR101420740B1 (ko) | 2005-12-13 | 2014-07-17 | 교또 다이가꾸 | 핵초기화 인자 |
| US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
| EP2137296A2 (en) | 2007-03-23 | 2009-12-30 | Wisconsin Alumni Research Foundation | Somatic cell reprogramming |
| WO2008122084A1 (en) | 2007-04-05 | 2008-10-16 | Johnson & Johnson Research Pty Limited | Nucleic acid enzymes and complexes and methods for their use |
| CN105861443A (zh) | 2007-04-07 | 2016-08-17 | 怀特黑德生物医学研究所 | 体细胞重编程 |
| CN101802172A (zh) | 2007-05-30 | 2010-08-11 | 通用医疗公司 | 由体细胞产生多能细胞的方法 |
| US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
| JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| US7615374B2 (en) | 2007-09-25 | 2009-11-10 | Wisconsin Alumni Research Foundation | Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions |
| EP2283117B1 (en) | 2008-04-21 | 2013-10-23 | Viacyte, Inc. | Methods for purifying pancreatic endoderm cells derived from human embryonic stem cells |
| US20100003674A1 (en) | 2008-07-03 | 2010-01-07 | Cope Frederick O | Adult stem cells, molecular signatures, and applications in the evaluation, diagnosis, and therapy of mammalian conditions |
| EP2313494A2 (en) | 2008-07-14 | 2011-04-27 | Oklahoma Medical Research Foundation | Production of pluripotent cells through inhibition of bright/arid3a function |
| US8278105B2 (en) | 2008-09-09 | 2012-10-02 | University Of Southern California | Induction, propagation and isolation of liver progenitor cells |
| US20120021519A1 (en) | 2008-09-19 | 2012-01-26 | Presidents And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
| EP3450545B1 (en) | 2008-10-24 | 2023-08-23 | Wisconsin Alumni Research Foundation | Pluripotent stem cells obtained by non-viral reprogramming |
| WO2010111422A2 (en) | 2009-03-25 | 2010-09-30 | The Salk Institute For Biological Studies | Induced pluripotent stem cell generation using two factors and p53 inactivation |
| WO2011008541A2 (en) | 2009-06-29 | 2011-01-20 | The Regents Of The University Of California | Molecular markers and assay methods for characterizing cells |
| WO2011046635A1 (en) | 2009-10-14 | 2011-04-21 | The Johns Hopkins University | Differentially methylated regions of reprogrammed induced pluripotent stem cells, method and compositions thereof |
| WO2011130624A2 (en) | 2010-04-16 | 2011-10-20 | Immune Disease Institute, Inc. | Sustained polypeptide expression from synthetic, modified rnas and uses thereof |
| US20130259842A1 (en) | 2010-05-18 | 2013-10-03 | Lee Rubin | Stable reprogrammed cells |
| US8945847B2 (en) | 2010-05-24 | 2015-02-03 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Methods and kits for ascertaining biosafety of an agent |
| JP2013545439A (ja) | 2010-09-17 | 2013-12-26 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 多能性幹細胞の有用性および安全性の特徴決定を行うための機能的ゲノミクスアッセイ |
| US20160115455A1 (en) | 2013-05-17 | 2016-04-28 | The Broad Institute, Inc. | Reprogrammed cells and methods of production and use thereof |
-
2014
- 2014-06-09 JP JP2016519572A patent/JP6697380B2/ja active Active
- 2014-06-09 EP EP14811445.7A patent/EP3008229B1/en active Active
- 2014-06-09 US US14/897,410 patent/US11085067B2/en active Active
- 2014-06-09 WO PCT/US2014/041513 patent/WO2014200905A2/en not_active Ceased
-
2018
- 2018-05-18 US US15/983,209 patent/US10626445B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US10626445B2 (en) | 2020-04-21 |
| WO2014200905A3 (en) | 2015-03-05 |
| US20180251818A1 (en) | 2018-09-06 |
| EP3008229B1 (en) | 2020-05-27 |
| US11085067B2 (en) | 2021-08-10 |
| US20160122803A1 (en) | 2016-05-05 |
| JP2016532435A (ja) | 2016-10-20 |
| EP3008229A2 (en) | 2016-04-20 |
| EP3008229A4 (en) | 2017-03-01 |
| WO2014200905A2 (en) | 2014-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6697380B2 (ja) | 多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ | |
| CN103459611B (zh) | 对多能干细胞的效用和安全性进行表征的功能基因组学研究 | |
| Janjic et al. | Prime-seq, efficient and powerful bulk RNA sequencing | |
| Guo et al. | Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst | |
| Percharde et al. | Hypertranscription in development, stem cells, and regeneration | |
| Wilson et al. | Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499 | |
| McDonald et al. | Defining the teratoma as a model for multi-lineage human development | |
| Abeyta et al. | Unique gene expression signatures of independently-derived human embryonic stem cell lines | |
| Ortmann et al. | Naive pluripotent stem cells exhibit phenotypic variability that is driven by genetic variation | |
| Rinaudo et al. | Effects of embryo culture on global pattern of gene expression in preimplantation mouse embryos | |
| Stadler et al. | Characterization of microRNAs involved in embryonic stem cell states | |
| Hall et al. | Oct4 and LIF/Stat3 additively induce Krüppel factors to sustain embryonic stem cell self-renewal | |
| WO2016103269A1 (en) | Populations of neural progenitor cells and methods of producing and using same | |
| Szczerbinska et al. | A chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state | |
| US20200080148A1 (en) | Cell characterisation | |
| Fu et al. | A temporal transcriptome and methylome in human embryonic stem cell-derived cardiomyocytes identifies novel regulators of early cardiac development | |
| Pozzi et al. | Genetic deletion of Hesx1 promotes exit from the pluripotent state and impairs developmental diapause | |
| Qiu et al. | Marmoset induced pluripotent stem cells: Robust neural differentiation following pretreatment with dimethyl sulfoxide | |
| Tsai et al. | Detection of transcriptional difference of porcine imprinted genes using different microarray platforms | |
| CN113106160B (zh) | 评估肝谱系细胞成熟度的标志物、双组学试剂盒及构建方法 | |
| Glenn et al. | A Pluripotent Stem Cell Platform for in Vitro Systems Genetics Studies of Mouse Development | |
| Simmons et al. | Rational, unbiased selection of reference genes for pluripotent stem cell-derived cardiomyocytes | |
| Gunji et al. | Global analysis of the expression patterns of transcriptional regulatory factors in formation of embryoid bodies using sensitive oligonucleotide microarray systems | |
| EP3218478B1 (en) | Predicting productivity in early cell line development | |
| Class et al. | Patent application title: FUNCTIONAL GENOMICS ASSAY FOR CHARACTERIZING PLURIPOTENT STEM CELL UTILITY AND SAFETY Inventors: Kevin C. Eggan (Boston, MA, US) Kevin C. Eggan (Boston, MA, US) Alexander Meissner (Cambridge, MA, US) Christoph Bock (Vienna, AT) Evangelos Kiskinis (Boston, MA, US) Griet Annie Frans Verstappen (Moltsel, BE) Assignees: President and Fellows of Harvard College |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170607 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20170607 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20180806 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20181102 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190205 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20190717 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20191015 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200116 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20200319 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20200417 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20200424 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6697380 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |