CN103703141B - 具有降低的非特异性活性的pcr反应混合物 - Google Patents

具有降低的非特异性活性的pcr反应混合物 Download PDF

Info

Publication number
CN103703141B
CN103703141B CN201280026623.5A CN201280026623A CN103703141B CN 103703141 B CN103703141 B CN 103703141B CN 201280026623 A CN201280026623 A CN 201280026623A CN 103703141 B CN103703141 B CN 103703141B
Authority
CN
China
Prior art keywords
polymerase
seq
nucleic acid
acid
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201280026623.5A
Other languages
English (en)
Chinese (zh)
Other versions
CN103703141A (zh
Inventor
程曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CN103703141A publication Critical patent/CN103703141A/zh
Application granted granted Critical
Publication of CN103703141B publication Critical patent/CN103703141B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201280026623.5A 2011-04-08 2012-02-10 具有降低的非特异性活性的pcr反应混合物 Active CN103703141B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161473710P 2011-04-08 2011-04-08
US61/473,710 2011-04-08
PCT/US2012/024735 WO2012138416A1 (en) 2011-04-08 2012-02-10 Pcr reaction mixtures with decreased non-specific activity

Publications (2)

Publication Number Publication Date
CN103703141A CN103703141A (zh) 2014-04-02
CN103703141B true CN103703141B (zh) 2016-08-17

Family

ID=46966404

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280026623.5A Active CN103703141B (zh) 2011-04-08 2012-02-10 具有降低的非特异性活性的pcr反应混合物

Country Status (6)

Country Link
US (2) US8916352B2 (enExample)
EP (1) EP2694670B1 (enExample)
JP (2) JP6072766B2 (enExample)
CN (1) CN103703141B (enExample)
SG (1) SG193990A1 (enExample)
WO (1) WO2012138416A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2820715C (en) * 2010-12-16 2020-03-31 Bio-Rad Laboratories, Inc. Universal reference dye for quantitative amplification
CN107075544B (zh) 2014-07-22 2021-01-29 生物辐射实验室股份有限公司 与聚合酶联用的缓冲液
CN108368151B (zh) 2015-12-07 2022-11-15 生物辐射实验室股份有限公司 二聚逆转录酶
US10273530B2 (en) 2016-01-15 2019-04-30 Thermo Fisher Scientific Baltics Uab Antibodies that bind thermophilic DNA polymerases
LT3402880T (lt) 2016-01-15 2024-03-12 Thermo Fisher Scientific Baltics Uab Termofiliniai dnr polimerazės mutantai
GB201704401D0 (en) * 2017-03-20 2017-05-03 Epistem Ltd Use of anticoagulants
CN110945141B (zh) * 2017-06-22 2023-08-08 生命技术公司 基于甲磺酸盐的预混液
US11618891B2 (en) 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
WO2019036682A1 (en) 2017-08-18 2019-02-21 Modernatx, Inc. RNA VARIANTS POLYMERASE
MX2021010075A (es) 2019-02-20 2021-12-10 Modernatx Inc Variantes de arn polimerasa para la formacion de casquetes cotranscripcionales.
CN112176042B (zh) * 2019-07-03 2023-10-17 赛纳生物科技(北京)有限公司 一种基因测序试剂及方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1500144A (zh) * 2001-02-06 2004-05-26 �����﹤����ʽ���� 扩增的核酸及其固定化制品
WO2010080910A1 (en) * 2009-01-08 2010-07-15 Bio-Rad Laboratories, Inc. Methods and compositions for improving efficiency of nucleic acids amplification reactions

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4246194A (en) 1977-12-08 1981-01-20 Research Organics, Inc. Amino-hydroxy-alkyl sulfonic acid-zwitterions
US4545985A (en) 1984-01-26 1985-10-08 The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services Pseudomonas exotoxin conjugate immunotoxins
US4751180A (en) 1985-03-28 1988-06-14 Chiron Corporation Expression using fused genes providing for protein product
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5679543A (en) 1985-08-29 1997-10-21 Genencor International, Inc. DNA sequences, vectors and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
US4935233A (en) 1985-12-02 1990-06-19 G. D. Searle And Company Covalently linked polypeptide cell modulators
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
DE4111393A1 (de) 1991-04-09 1992-10-15 Behringwerke Ag Stabilisierte faktor viii-praeparationen
US5962292A (en) 1993-09-07 1999-10-05 Kyowa Hakko Kogyo Co., Ltd. Process for producing cis-3-hydroxy-L-proline
US6063566A (en) 1994-05-13 2000-05-16 The Scripps Research Institute Catalytic RNA molecules
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US6413747B1 (en) * 1994-10-03 2002-07-02 Shimadzu Corporation Enhancement of nucleic acid amplification by the addition of a polyamine
US5948614A (en) 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US6037128A (en) * 1997-03-31 2000-03-14 Council Of Scientific & Industrial Research Process for the preparation of semisynthetic amplicon useful for sex determination of the papaya plant
US5882904A (en) 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
DK2130554T3 (da) 1999-02-22 2012-12-03 Univ Connecticut Albuminfrie faktor VIII-præparater
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
ES2311638T5 (es) 2001-11-28 2011-09-08 Bio-Rad Laboratories, Inc. Métodos de uso de polimerasas mejoradas.
DE60235491D1 (de) 2001-11-28 2010-04-08 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
CA2518588A1 (en) * 2002-07-25 2004-02-05 Mj Bioworks Incorporated Hybrid dna polymerase proteins having increased polymerase to exonuclease activity
US7560260B2 (en) * 2002-07-25 2009-07-14 Bio-Rad Laboratories, Inc. Compositions with polymerase activity
US7666645B2 (en) * 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
US7704712B2 (en) 2003-03-25 2010-04-27 Stratagene California DNA polymerase fusions and uses thereof
CA2488224A1 (en) * 2003-04-03 2004-10-21 Alnylam Pharmaceuticals, Inc. Irna conjugates
US20040214194A1 (en) 2003-04-17 2004-10-28 Mj Bioworks, Inc. Methods of making hybrid proteins
US20070117094A1 (en) * 2003-09-30 2007-05-24 Yoshihide Hayashizaki Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes
WO2005076908A2 (en) 2004-02-04 2005-08-25 Eppendorf Ag Anti-freeze protein enhanced nucleic acid amplification
JP4972541B2 (ja) 2004-04-01 2012-07-11 バイオ−ラッド ラボラトリーズ インコーポレーティッド 標識されたプローブおよび３’→５’エキソヌクレアーゼ活性を用いた定量的増幅方法
US20050277121A1 (en) 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20060005174A1 (en) 2004-07-01 2006-01-05 International Business Machines Corporation Defining hierarchical structures with markup languages and reflection
US20060089324A1 (en) * 2004-10-22 2006-04-27 Sailen Barik RNAi modulation of RSV, PIV and other respiratory viruses and uses thereof
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
EP2052079A2 (en) * 2006-07-17 2009-04-29 Sirna Therapeutics Inc. Rna interference mediated inhibition of proprotein convertase subtilisin kexin 9 (pcsk9) gene expression using short interfering nucleic acid (sina)
DK2056845T3 (da) * 2006-08-08 2017-11-27 Rheinische Friedrich-Wilhelms-Universität Bonn Struktur og anvendelse af 5'-phosphat-oligonukleotider
EP2476689B1 (en) * 2007-05-10 2015-10-21 Agilent Technologies, Inc. Thiocarbon-protecting groups for RNA synthesis
AR066984A1 (es) * 2007-06-15 2009-09-23 Novartis Ag Inhibicion de la expresion de la subunidad alfa del canal epitelial de sodio (enac) por medio de arni (arn de interferencia)
JP5702148B2 (ja) * 2007-11-27 2015-04-15 バイオ−ラッド ラボラトリーズ，インコーポレイティド １ステップｒｔ−ｐｃｒの阻害低減
CN102803483B (zh) * 2009-06-12 2014-04-30 精密公司 用于微流体装置中taq 聚合酶的干燥储存的可再水合基质
EP2652147B1 (en) 2010-12-17 2017-01-25 Wisconsin Alumni Research Foundation One-step method of elution of dna from blood samples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1500144A (zh) * 2001-02-06 2004-05-26 �����﹤����ʽ���� 扩增的核酸及其固定化制品
WO2010080910A1 (en) * 2009-01-08 2010-07-15 Bio-Rad Laboratories, Inc. Methods and compositions for improving efficiency of nucleic acids amplification reactions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Spermidine facilitates PCR amplification of target DNA;Wan et al;《PCR Methods and Application》;19931201;第3卷(第3期);第210页左栏倒数第1-2段 *

Also Published As

Publication number Publication date
US20120258500A1 (en) 2012-10-11
US9556423B2 (en) 2017-01-31
JP2017029161A (ja) 2017-02-09
US8916352B2 (en) 2014-12-23
EP2694670A4 (en) 2015-04-29
JP2014511692A (ja) 2014-05-19
EP2694670B1 (en) 2017-07-19
US20150031107A1 (en) 2015-01-29
WO2012138416A1 (en) 2012-10-11
JP6072766B2 (ja) 2017-02-01
SG193990A1 (en) 2013-11-29
CN103703141A (zh) 2014-04-02
EP2694670A1 (en) 2014-02-12

Similar Documents

Publication Publication Date Title
CN103703141B (zh) 具有降低的非特异性活性的pcr反应混合物
US10787694B2 (en) Methods and compositions for improving efficiency of nucleic acids amplification reactions
CN103562410B (zh) 具有降低的非特异性活性的sso7-聚合酶缀合物
CN103620049B (zh) 具有产生长扩增子的能力的杂合聚合酶
CN105452451B (zh) 融合聚合酶
EP3172332B1 (en) Buffers for use with polymerases

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant