JP2018538007A5 - - Google Patents

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JP2018538007A5
JP2018538007A5 JP2018543065A JP2018543065A JP2018538007A5 JP 2018538007 A5 JP2018538007 A5 JP 2018538007A5 JP 2018543065 A JP2018543065 A JP 2018543065A JP 2018543065 A JP2018543065 A JP 2018543065A JP 2018538007 A5 JP2018538007 A5 JP 2018538007A5
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JP2018543065A
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JP2018538007A (ja
JP7319646B2 (ja
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Priority claimed from PCT/US2016/060650 external-priority patent/WO2017079636A1/en
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JP2018543065A 2015-11-05 2016-11-04 エクストリーム逆転写pcr Active JP7319646B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562251400P 2015-11-05 2015-11-05
US62/251,400 2015-11-05
PCT/US2016/060650 WO2017079636A1 (en) 2015-11-05 2016-11-04 Extreme reverse transcription pcr

Publications (3)

Publication Number Publication Date
JP2018538007A JP2018538007A (ja) 2018-12-27
JP2018538007A5 true JP2018538007A5 (enExample) 2019-12-12
JP7319646B2 JP7319646B2 (ja) 2023-08-02

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JP2018543065A Active JP7319646B2 (ja) 2015-11-05 2016-11-04 エクストリーム逆転写pcr

Country Status (9)

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US (1) US10900074B2 (enExample)
EP (1) EP3337615B1 (enExample)
JP (1) JP7319646B2 (enExample)
CN (1) CN108367287B (enExample)
CA (1) CA3000824C (enExample)
DK (1) DK3337615T3 (enExample)
ES (1) ES2916153T3 (enExample)
PL (1) PL3337615T3 (enExample)
WO (1) WO2017079636A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018023360A (ja) * 2016-07-29 2018-02-15 セイコーエプソン株式会社 核酸増幅反応方法、試薬、および試薬の使用方法
WO2018195668A1 (en) * 2017-04-28 2018-11-01 Spartan Bioscience Inc. Direct nucleic acid analysis of environmental and biological samples
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
US20220064707A1 (en) * 2019-01-17 2022-03-03 Northwestern University Rapid reverse transcription quantitative polymerase chain reaction
WO2020175406A1 (ja) * 2019-02-28 2020-09-03 池田食研株式会社 遺伝子増幅法及び遺伝子増幅用キット
SG10201905641YA (en) * 2019-06-19 2021-01-28 Delta Electronics Int’L Singapore Pte Ltd Primer pair, kit and method for detecting zika virus
US11730193B2 (en) 2019-12-15 2023-08-22 Shaheen Innovations Holding Limited Hookah device
US11254979B2 (en) 2020-06-01 2022-02-22 Shaheen Innovations Holding Limited Systems and devices for infectious disease screening
EP4087941A4 (en) 2020-01-10 2023-06-28 Siemens Healthcare Diagnostics, Inc. Compositions, kits, and methods for performing rapid polymerase chain reactions
JP7580877B2 (ja) * 2020-03-30 2024-11-12 国立研究開発法人産業技術総合研究所 核酸増幅用組成物
US12687470B2 (en) 2020-04-06 2026-07-21 Shaheen Innovations Holding Limited Cell lysis systems and methods
AU2021285405B2 (en) 2020-06-01 2026-06-18 Shaheen Innovations Holding Limited An infectious disease screening system
IL298682B2 (en) 2020-06-01 2026-02-01 Shaheen Innovations Holding Ltd Infectious disease scanning device
GB202115637D0 (en) * 2021-11-01 2021-12-15 Anglia Ruskin Univ Higher Education Corporation Nucleic acid amplification method
US20250051836A1 (en) * 2021-12-21 2025-02-13 Qiagen Gmbh Method for amplifying rna in a sample
CN114885450B (zh) * 2022-07-11 2022-09-20 中国飞机强度研究所 一种空天飞机测试用的极高温极低温热强度循环试验系统
WO2025049638A1 (en) * 2023-08-29 2025-03-06 Access Medical Systems, Ltd. Devices for rapid amplifying nucleic acid and method of using the same
CN117363620B (zh) * 2023-12-08 2024-03-01 北京全式金生物技术股份有限公司 一种特异性结合反转录酶的rna适配体及其应用
CN119876354B (zh) * 2025-01-24 2026-01-27 申联生物医药(上海)股份有限公司 一种全预混式荧光pcr试剂盒及稳定剂

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2065719A1 (en) 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
AU7551594A (en) 1993-07-29 1995-02-28 MURASHIGE, Kate H. Method for recognition of the nucleotide sequence of a purified dna segment
CA2223078C (en) 1995-06-07 2008-11-25 Gilead Sciences, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
WO1997011085A1 (en) * 1995-09-19 1997-03-27 University Of Massachusetts Inhibited biological degradation of oligodeoxynucleotides
US6210882B1 (en) 1998-01-29 2001-04-03 Mayo Foundation For Medical Education And Reseach Rapid thermocycling for sample analysis
US6300073B1 (en) * 1999-10-01 2001-10-09 Clontech Laboratories, Inc. One step RT-PCR methods, enzyme mixes and kits for use in practicing the same
KR20030073875A (ko) 2002-03-13 2003-09-19 주식회사 하이닉스반도체 반도체소자의 소자분리패턴 형성방법
US8232091B2 (en) 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ.ホフマン−ラ ロシュ アーゲー 急速ワンステップrt−pcr
DE102008008313A1 (de) * 2008-02-07 2009-08-13 Qiagen Gmbh Amplifikation von bisulfitierten Nukleinsäuren
WO2009105499A1 (en) 2008-02-20 2009-08-27 Termaat Joel R Thermocycler and sample vessel for rapid amplification of dna
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
EP3150727B1 (en) 2008-04-30 2019-07-10 Integrated DNA Technologies Inc. Rnase-h-based assays utilizing modified rna monomers
US8349563B2 (en) 2008-11-18 2013-01-08 Life Technologies Corporation Sequence amplification with target primers
CN101407850B (zh) 2008-11-20 2011-01-05 江苏出入境检验检疫局动植物与食品检测中心 猴痘病毒的pcr快速检测试剂盒及检测方法
PT2539472E (pt) 2010-02-26 2015-09-24 Life Technologies Corp Pcr rápida para genotipagem de str
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
WO2011163120A1 (en) * 2010-06-21 2011-12-29 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
EP2651560B1 (en) 2010-12-17 2020-03-18 BJS IP Limited Methods and systems for fast pcr heating
DK2855692T3 (da) * 2012-05-24 2019-10-14 Univ Utah Res Found Ekstrem pcr
US9353409B2 (en) 2013-01-15 2016-05-31 Jun Euihum Lee Compositions and methods for RT-PCR
WO2015069743A1 (en) 2013-11-05 2015-05-14 Biofire Diagnostics, Llc Induction pcr

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