GB1510926A - Chromogenic thrombin substrates - Google Patents

Chromogenic thrombin substrates

Info

Publication number
GB1510926A
GB1510926A GB25580/76A GB2558076A GB1510926A GB 1510926 A GB1510926 A GB 1510926A GB 25580/76 A GB25580/76 A GB 25580/76A GB 2558076 A GB2558076 A GB 2558076A GB 1510926 A GB1510926 A GB 1510926A
Authority
GB
United Kingdom
Prior art keywords
substrate
enzymes
formula
amount
thrombin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB25580/76A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phadia AB
Original Assignee
Kabi AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabi AB filed Critical Kabi AB
Publication of GB1510926A publication Critical patent/GB1510926A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/064—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for omega-amino or -guanidino functions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0812—Tripeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/30—Naphthyl amides, e.g. beta-NA, 2-NA, 4-methoxy-beta-naphthylamine, i.e. 4MNA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Neurosurgery (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
GB25580/76A 1975-07-11 1976-06-21 Chromogenic thrombin substrates Expired GB1510926A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE7507975-6A SE407405B (sv) 1975-07-11 1975-07-11 Nya kromogena trombinsubstrat

Publications (1)

Publication Number Publication Date
GB1510926A true GB1510926A (en) 1978-05-17

Family

ID=20325116

Family Applications (1)

Application Number Title Priority Date Filing Date
GB25580/76A Expired GB1510926A (en) 1975-07-11 1976-06-21 Chromogenic thrombin substrates

Country Status (22)

Country Link
JP (1) JPS5224590A (sv)
AT (1) AT348686B (sv)
AU (1) AU497547B2 (sv)
BE (1) BE843970A (sv)
CA (1) CA1068261A (sv)
CH (1) CH622287A5 (sv)
CS (1) CS196314B2 (sv)
DD (1) DD128691A5 (sv)
DE (1) DE2629195C3 (sv)
DK (1) DK145799C (sv)
ES (1) ES449739A1 (sv)
FI (1) FI56525C (sv)
FR (1) FR2317280A1 (sv)
GB (1) GB1510926A (sv)
IL (1) IL49829A (sv)
IT (1) IT1062605B (sv)
NL (1) NL188355C (sv)
NO (1) NO142576C (sv)
PL (1) PL103003B1 (sv)
SE (1) SE407405B (sv)
SU (1) SU719492A3 (sv)
ZA (1) ZA763586B (sv)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2140423A (en) * 1983-04-28 1984-11-28 Kimberly Clark Co A method capable of determining cathopsin B in the presence of other proteolytic enzymes and compounds useful therefor

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5255593A (en) * 1975-10-30 1977-05-07 Ajinomoto Kk Measuring method of enzyme activity
DE2757992A1 (de) * 1977-12-24 1979-06-28 Boehringer Mannheim Gmbh Verfahren zur prothrombin-bestimmung
DE2812943C3 (de) * 1978-03-23 1981-05-14 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Reagens zur Bestimmung der biologischen Aktivität von Heparin im Plasma
US4289498A (en) * 1979-01-08 1981-09-15 Ortho Diagnostics, Inc. One-stage prothrombin assay and compositions useful therein
FR2471410A2 (fr) * 1979-12-17 1981-06-19 Jozefonvicz Marcel Perfectionnements apportes a un procede de dosage des proteases et des anti-proteases et notamment des proteases et antiproteases des systemes de la coagulation et du complement
EP0018002B1 (de) * 1979-04-24 1983-02-09 Marcel Jozefonvicz Neues Bestimmungsverfahren für Proteasen und Antiproteasen
DE3005540A1 (de) * 1980-02-14 1981-08-20 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagens zur bestimmung der biologischen aktivitaet von heparin im plasma
DK155051C (da) * 1980-05-06 1989-07-03 Pentapharm Ag Tripeptidderivater samt fremgangsmaade til kvantitativ analyse for proteolytiske enzymer under anvendelse deraf
JPS5856695A (ja) * 1981-09-28 1983-04-04 Nitto Boseki Co Ltd 新規なトロンビン測定用基質
DE3211254A1 (de) * 1982-03-26 1983-09-29 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zum nachweis des vorliegens einer allergie und zum spezifischen nachweis des fuer die allergie verantwortlichen allergens
US20090075298A1 (en) 2005-05-20 2009-03-19 Mitsubishi Kagaku Iatron, Inc. Method of analyzing enzyme

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE380257B (sv) * 1972-05-02 1975-11-03 Bofors Ab Nya diagnostiskt verksamma substrat med hog specificitet till trombin och andra proteolytiska enzymer av typen peptidyl-peptid-hydrolaser
IL42124A (en) * 1972-05-02 1977-02-28 Kabi Ab Substrate for the determination of proteolytic enzymes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2140423A (en) * 1983-04-28 1984-11-28 Kimberly Clark Co A method capable of determining cathopsin B in the presence of other proteolytic enzymes and compounds useful therefor

Also Published As

Publication number Publication date
NL188355C (nl) 1992-06-01
FI56525C (fi) 1980-02-11
ZA763586B (en) 1977-05-25
FR2317280A1 (fr) 1977-02-04
CS196314B2 (en) 1980-03-31
SU719492A3 (ru) 1980-02-29
BE843970A (fr) 1976-11-03
IL49829A0 (en) 1976-08-31
DD128691A5 (de) 1977-12-07
ATA463976A (de) 1978-07-15
PL103003B1 (pl) 1979-05-31
NL7607104A (nl) 1977-01-13
DK145799C (da) 1983-08-29
DE2629195B2 (de) 1980-01-10
NL188355B (nl) 1992-01-02
DK145799B (da) 1983-03-07
DE2629195C3 (de) 1983-11-17
IT1062605B (it) 1984-10-20
JPS5615239B2 (sv) 1981-04-09
FR2317280B1 (sv) 1980-05-16
AU497547B2 (en) 1978-12-14
IL49829A (en) 1978-12-17
JPS5224590A (en) 1977-02-24
CA1068261A (en) 1979-12-18
ES449739A1 (es) 1978-01-16
NO142576C (no) 1980-09-10
CH622287A5 (en) 1981-03-31
DE2629195A1 (de) 1977-01-13
AT348686B (de) 1979-02-26
NO762407L (sv) 1977-01-12
DK312676A (da) 1977-01-12
FI56525B (fi) 1979-10-31
NO142576B (no) 1980-06-02
FI762010A (sv) 1977-01-12
SE407405B (sv) 1979-03-26
SE7507975L (sv) 1977-01-12
AU1530576A (en) 1978-01-05

Similar Documents

Publication Publication Date Title
GB1510926A (en) Chromogenic thrombin substrates
ES442997A1 (es) Procedimiento de obtencion de substratos cromogenicos para enzimas.
ATE117311T1 (de) Substrate für hydrolasen, verfahren zu ihrer herstellung und ihre verwendung.
SE7705303L (sv) Forfarande for bestemning av substrat eller enzymaktiviteter medelst en redox-reaktion
IT1070002B (it) Substrato cromogeno o fluorescente impiegabile come reagente per la determinazione quantitativa di enzimi proteolitici del gruppo e.c. 3.4.4.
JPS5655361A (en) Chromogen compound
DE69223289D1 (de) Einstufentest für aspartat-aminotransferase
BE843969A (fr) Nouveaux substrats chromogenes d'enzymes
ATE147790T1 (de) Direkte methode zum nachweis von sehr niedrigerem grad der coliform-kontamination
ES8104417A1 (es) Un metodo de determinacion de la presencia de un enzima en un analizado
IT1117757B (it) Substrato analitico fluorogeno per enzimi proteolitici
ATE239798T1 (de) Identifikation von salmonella
ATE1296T1 (de) Substratzusammensetzung und ihre verwendung.
SE8404930D0 (sv) Aqueous compositions containing stabilized enzymes
GB1404195A (en) Process employing 4-methoxy-2-naphthylamides for determining enzyme concentrations
ATE126542T1 (de) Verwendung von 1-arylsemicarbaziden zur stabilisierung von enzymsubstraten, entsprechende verfahren und diagnositsches mittel enthaltend einen solchen stabilisator.
ES8701842A1 (es) Procedimiento para la determinacion fotometrica de procalicreina en el plasma
FI97152C (sv) Fluorogeniska tryptofanassubstrat
ES8708261A1 (es) Un procedimiento para determinar la actividad de una hidrolasa macromolecular
DK0476930T3 (da) Enzymanalyser.
JPS5461128A (en) Peptide derivative
Svendsen Standardization of Chromogenic Synthetic Peptide Substrates for Proteolytic Enzymes
JPS57146771A (en) Coumarin derivative
ES479473A1 (es) Procedimiento para la obtencion de esteres del acido n-car- boxiacilaminico.
JPS5753445A (en) Peptide compound

Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years

Effective date: 19960620