ES8708261A1 - Un procedimiento para determinar la actividad de una hidrolasa macromolecular - Google Patents

Un procedimiento para determinar la actividad de una hidrolasa macromolecular

Info

Publication number
ES8708261A1
ES8708261A1 ES552374A ES552374A ES8708261A1 ES 8708261 A1 ES8708261 A1 ES 8708261A1 ES 552374 A ES552374 A ES 552374A ES 552374 A ES552374 A ES 552374A ES 8708261 A1 ES8708261 A1 ES 8708261A1
Authority
ES
Spain
Prior art keywords
assay
macromolecular
fluorescence polarization
hydrolases
polarization assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES552374A
Other languages
English (en)
Other versions
ES552374A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of ES8708261A1 publication Critical patent/ES8708261A1/es
Publication of ES552374A0 publication Critical patent/ES552374A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
  • Detergent Compositions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

PROCEDIMIENTO PARA DETERMINAR LA ACTIVIDAD DE UNA HIDROLASA MACROMOLECULAR. COMPRENDE: A) PREPARAR UNA SOLUCION DE ENSAYO DE UNA MUESTRA SOSPECHOSA DE CONTENER HIDROLASA MACROMOLECULAR Y UN SUSTRATO MARCADO CON UN FLUOROFORO, SUSCEPTIBLE DE SER ESCONDIDO POR LA HIDROLASA, SIENDO LA CONCENTRACION DE 0,01 A 300 MG/ML; B) INCUBAR LA SOLUCION DE ENSAYO DURANTE 1 MINUTO A 1 HORA, A UNA TEMPERATURA ENTRE 15GC Y 45GC, MANTENIENDOSE EL PH ENTRE 3 Y 11; C) HACER PASAR LUZ POLARIZADA PLANA A TRAVES DE LA SOLUCION DE LA ETAPA C) COMO INDICACION DE LA ACTIVIDAD DE LA HIDROLASA EN LA MUESTRA COMPRENDIENDO EL SUSTRATO AMILOSA, GELATINA, HEMOGLOBINA, CASEINA, O LIPOPROTEINA.
ES552374A 1985-02-25 1986-02-25 Un procedimiento para determinar la actividad de una hidrolasa macromolecular Expired ES8708261A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US70526885A 1985-02-25 1985-02-25

Publications (2)

Publication Number Publication Date
ES8708261A1 true ES8708261A1 (es) 1987-10-01
ES552374A0 ES552374A0 (es) 1987-10-01

Family

ID=24832729

Family Applications (2)

Application Number Title Priority Date Filing Date
ES552374A Expired ES8708261A1 (es) 1985-02-25 1986-02-25 Un procedimiento para determinar la actividad de una hidrolasa macromolecular
ES557135A Expired ES8801518A1 (es) 1985-02-25 1986-10-10 Un procedimiento para la preparacion de un reactivo para un ensayo de polarizacion de la fluorescencia para determinar una hidrolasa macromolecular

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES557135A Expired ES8801518A1 (es) 1985-02-25 1986-10-10 Un procedimiento para la preparacion de un reactivo para un ensayo de polarizacion de la fluorescencia para determinar una hidrolasa macromolecular

Country Status (7)

Country Link
EP (1) EP0194472B1 (es)
JP (1) JPH0687795B2 (es)
AT (1) ATE78517T1 (es)
AU (1) AU596574B2 (es)
CA (1) CA1279242C (es)
DE (1) DE3686071T2 (es)
ES (2) ES8708261A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8721302D0 (en) * 1987-09-10 1987-10-14 London King S College Substrates for assay of enzymes
SE8801702D0 (sv) * 1988-05-05 1988-05-05 Pharmacia Ab Sett vid bestemning av enzymatisk aktivitet
GB9100833D0 (en) * 1991-01-15 1991-02-27 Univ Alberta Methods for assaying proteinases and proteinase inhibitors
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
US5976822A (en) * 1995-05-18 1999-11-02 Coulter International Corp. Method and reagent for monitoring apoptosis and distinguishing apoptosis from necrosis
US5804395A (en) * 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
PT1058738E (pt) * 1998-03-06 2002-05-31 Novozymes As Metodo de seleccao por via de polarizacao de fluorescencia
JP2006025625A (ja) * 2004-07-12 2006-02-02 Seikagaku Kogyo Co Ltd グリコサミノグリカン分解酵素の活性測定方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3063915A (en) * 1958-10-17 1962-11-13 Miles Lab Diagnostic composition and method for determining protease activity
GB1381380A (en) * 1972-09-06 1975-01-22 Warner Lambert Co Water-soluble dyed substrate for amylase assay
DE2363854C3 (de) * 1973-12-21 1980-01-03 Behringwerke Ag, 3550 Marburg Substrat zur Proteinasebestimmung sowie dessen Herstellung

Also Published As

Publication number Publication date
ATE78517T1 (de) 1992-08-15
AU5381086A (en) 1986-08-28
DE3686071D1 (de) 1992-08-27
JPH0687795B2 (ja) 1994-11-09
ES557135A0 (es) 1988-01-16
EP0194472A3 (en) 1988-10-05
AU596574B2 (en) 1990-05-10
EP0194472A2 (en) 1986-09-17
CA1279242C (en) 1991-01-22
ES8801518A1 (es) 1988-01-16
ES552374A0 (es) 1987-10-01
JPS61234797A (ja) 1986-10-20
EP0194472B1 (en) 1992-07-22
DE3686071T2 (de) 1993-01-07

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970401