ATE78517T1 - Polarisierter fluoreszenztest von makromolekularen hydrolasen. - Google Patents

Polarisierter fluoreszenztest von makromolekularen hydrolasen.

Info

Publication number
ATE78517T1
ATE78517T1 AT86102035T AT86102035T ATE78517T1 AT E78517 T1 ATE78517 T1 AT E78517T1 AT 86102035 T AT86102035 T AT 86102035T AT 86102035 T AT86102035 T AT 86102035T AT E78517 T1 ATE78517 T1 AT E78517T1
Authority
AT
Austria
Prior art keywords
macromolecular
hydrolase
assay
fluorescence test
polarized fluorescence
Prior art date
Application number
AT86102035T
Other languages
English (en)
Inventor
Mark Raymond Shaffar
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Application granted granted Critical
Publication of ATE78517T1 publication Critical patent/ATE78517T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Luminescent Compositions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT86102035T 1985-02-25 1986-02-18 Polarisierter fluoreszenztest von makromolekularen hydrolasen. ATE78517T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70526885A 1985-02-25 1985-02-25
EP86102035A EP0194472B1 (de) 1985-02-25 1986-02-18 Polarisierter Fluoreszenztest von makromolekularen Hydrolasen.

Publications (1)

Publication Number Publication Date
ATE78517T1 true ATE78517T1 (de) 1992-08-15

Family

ID=24832729

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86102035T ATE78517T1 (de) 1985-02-25 1986-02-18 Polarisierter fluoreszenztest von makromolekularen hydrolasen.

Country Status (7)

Country Link
EP (1) EP0194472B1 (de)
JP (1) JPH0687795B2 (de)
AT (1) ATE78517T1 (de)
AU (1) AU596574B2 (de)
CA (1) CA1279242C (de)
DE (1) DE3686071T2 (de)
ES (2) ES8708261A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8721302D0 (en) * 1987-09-10 1987-10-14 London King S College Substrates for assay of enzymes
SE8801702D0 (sv) * 1988-05-05 1988-05-05 Pharmacia Ab Sett vid bestemning av enzymatisk aktivitet
GB9100833D0 (en) * 1991-01-15 1991-02-27 Univ Alberta Methods for assaying proteinases and proteinase inhibitors
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
US5976822A (en) * 1995-05-18 1999-11-02 Coulter International Corp. Method and reagent for monitoring apoptosis and distinguishing apoptosis from necrosis
US5804395A (en) * 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
ATE208828T1 (de) * 1998-03-06 2001-11-15 Novozymes As Ein screeningverfahren, basierend auf fluoreszenzpolarisation
JP2006025625A (ja) * 2004-07-12 2006-02-02 Seikagaku Kogyo Co Ltd グリコサミノグリカン分解酵素の活性測定方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3063915A (en) * 1958-10-17 1962-11-13 Miles Lab Diagnostic composition and method for determining protease activity
GB1381380A (en) * 1972-09-06 1975-01-22 Warner Lambert Co Water-soluble dyed substrate for amylase assay
DE2363854C3 (de) * 1973-12-21 1980-01-03 Behringwerke Ag, 3550 Marburg Substrat zur Proteinasebestimmung sowie dessen Herstellung

Also Published As

Publication number Publication date
EP0194472A2 (de) 1986-09-17
EP0194472A3 (en) 1988-10-05
ES8708261A1 (es) 1987-10-01
DE3686071D1 (de) 1992-08-27
ES8801518A1 (es) 1988-01-16
CA1279242C (en) 1991-01-22
ES552374A0 (es) 1987-10-01
AU5381086A (en) 1986-08-28
JPS61234797A (ja) 1986-10-20
JPH0687795B2 (ja) 1994-11-09
AU596574B2 (en) 1990-05-10
DE3686071T2 (de) 1993-01-07
ES557135A0 (es) 1988-01-16
EP0194472B1 (de) 1992-07-22

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee