DE3686071T2 - Polarisierter fluoreszenztest von makromolekularen hydrolasen. - Google Patents

Polarisierter fluoreszenztest von makromolekularen hydrolasen.

Info

Publication number
DE3686071T2
DE3686071T2 DE8686102035T DE3686071T DE3686071T2 DE 3686071 T2 DE3686071 T2 DE 3686071T2 DE 8686102035 T DE8686102035 T DE 8686102035T DE 3686071 T DE3686071 T DE 3686071T DE 3686071 T2 DE3686071 T2 DE 3686071T2
Authority
DE
Germany
Prior art keywords
macromolecular
assay
hydrolases
fluorescence test
polarized fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686102035T
Other languages
English (en)
Other versions
DE3686071D1 (de
Inventor
Mark Raymond Shaffar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of DE3686071D1 publication Critical patent/DE3686071D1/de
Application granted granted Critical
Publication of DE3686071T2 publication Critical patent/DE3686071T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Luminescent Compositions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE8686102035T 1985-02-25 1986-02-18 Polarisierter fluoreszenztest von makromolekularen hydrolasen. Expired - Fee Related DE3686071T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US70526885A 1985-02-25 1985-02-25

Publications (2)

Publication Number Publication Date
DE3686071D1 DE3686071D1 (de) 1992-08-27
DE3686071T2 true DE3686071T2 (de) 1993-01-07

Family

ID=24832729

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686102035T Expired - Fee Related DE3686071T2 (de) 1985-02-25 1986-02-18 Polarisierter fluoreszenztest von makromolekularen hydrolasen.

Country Status (7)

Country Link
EP (1) EP0194472B1 (de)
JP (1) JPH0687795B2 (de)
AT (1) ATE78517T1 (de)
AU (1) AU596574B2 (de)
CA (1) CA1279242C (de)
DE (1) DE3686071T2 (de)
ES (2) ES8708261A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8721302D0 (en) * 1987-09-10 1987-10-14 London King S College Substrates for assay of enzymes
SE8801702D0 (sv) * 1988-05-05 1988-05-05 Pharmacia Ab Sett vid bestemning av enzymatisk aktivitet
GB9100833D0 (en) * 1991-01-15 1991-02-27 Univ Alberta Methods for assaying proteinases and proteinase inhibitors
US5445935A (en) * 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
US5976822A (en) * 1995-05-18 1999-11-02 Coulter International Corp. Method and reagent for monitoring apoptosis and distinguishing apoptosis from necrosis
US5804395A (en) * 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
ATE208828T1 (de) * 1998-03-06 2001-11-15 Novozymes As Ein screeningverfahren, basierend auf fluoreszenzpolarisation
JP2006025625A (ja) * 2004-07-12 2006-02-02 Seikagaku Kogyo Co Ltd グリコサミノグリカン分解酵素の活性測定方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3063915A (en) * 1958-10-17 1962-11-13 Miles Lab Diagnostic composition and method for determining protease activity
GB1381380A (en) * 1972-09-06 1975-01-22 Warner Lambert Co Water-soluble dyed substrate for amylase assay
DE2363854C3 (de) * 1973-12-21 1980-01-03 Behringwerke Ag, 3550 Marburg Substrat zur Proteinasebestimmung sowie dessen Herstellung

Also Published As

Publication number Publication date
EP0194472A2 (de) 1986-09-17
EP0194472A3 (en) 1988-10-05
ES8708261A1 (es) 1987-10-01
DE3686071D1 (de) 1992-08-27
ES8801518A1 (es) 1988-01-16
CA1279242C (en) 1991-01-22
ES552374A0 (es) 1987-10-01
AU5381086A (en) 1986-08-28
JPS61234797A (ja) 1986-10-20
ATE78517T1 (de) 1992-08-15
JPH0687795B2 (ja) 1994-11-09
AU596574B2 (en) 1990-05-10
ES557135A0 (es) 1988-01-16
EP0194472B1 (de) 1992-07-22

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee