ES8104417A1 - Un metodo de determinacion de la presencia de un enzima en un analizado - Google Patents
Un metodo de determinacion de la presencia de un enzima en un analizadoInfo
- Publication number
- ES8104417A1 ES8104417A1 ES490788A ES490788A ES8104417A1 ES 8104417 A1 ES8104417 A1 ES 8104417A1 ES 490788 A ES490788 A ES 490788A ES 490788 A ES490788 A ES 490788A ES 8104417 A1 ES8104417 A1 ES 8104417A1
- Authority
- ES
- Spain
- Prior art keywords
- substrates
- determining
- proteolytic enzymes
- amino
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000758 substrate Substances 0.000 title abstract 2
- 102000035195 Peptidases Human genes 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 title 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 title 1
- 239000012530 fluid Substances 0.000 abstract 2
- JBNOVHJXQSHGRL-UHFFFAOYSA-N 7-amino-4-(trifluoromethyl)coumarin Chemical compound FC(F)(F)C1=CC(=O)OC2=CC(N)=CC=C21 JBNOVHJXQSHGRL-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 150000003862 amino acid derivatives Chemical class 0.000 abstract 1
- 239000013060 biological fluid Substances 0.000 abstract 1
- 239000003593 chromogenic compound Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/06—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2
- C07D311/08—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring
- C07D311/16—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring substituted in position 7
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/06—Dipeptides
- C07K5/06191—Dipeptides containing heteroatoms different from O, S, or N
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0827—Tripeptides containing heteroatoms different from O, S, or N
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/20—Coumarin derivatives
- C12Q2337/24—7-Amino-4-trifluoromethylcoumarin, i.e. AFC
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/28—Bound to a nonpeptide drug, nonpeptide label, nonpeptide carrier, or a nonpeptide resin
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODO PARA DETERMINAR LA PRESENCIA DE ENZIMAS PROTEOLITICOS EN UN HUMOR BIOLOGICO. CONSISTE EN COMBINAR EL HUMOR CON UN SUSTRATO DE FORMULA (I), QUE PUEDE SER HIDROLIZADO POR EL ENZIMA PARA LIBERAR 7-AMINO-4-TRIFLUORMETILCUMARINA, SIENDO R UN AMINOACIDO, UN PEPTIDO O UN DERIVADO DE ELLOS. A CONTINUACION SE INCUBA LA MEZCLA, PERMITIENDO LA REACCION DE HIDROLISIS PARA FORMAR UN HIDROLIZADO ENZIMATICO, DETERMINANDO, POR ULTIMO, LA PRESENCIA DE 7-AMINO-4-TRIFLUORMETILCUMARINA EN EL POR MEDIOS FLUOROMETRICOS O ESPECTROFOTOMETRICOS. *FORMULA*
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/032,444 US4294923A (en) | 1979-04-23 | 1979-04-23 | Substrates and method for determining enzymes |
Publications (2)
Publication Number | Publication Date |
---|---|
ES490788A0 ES490788A0 (es) | 1981-04-16 |
ES8104417A1 true ES8104417A1 (es) | 1981-04-16 |
Family
ID=21864999
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES490788A Expired ES8104417A1 (es) | 1979-04-23 | 1980-04-22 | Un metodo de determinacion de la presencia de un enzima en un analizado |
ES497640A Expired ES8200675A1 (es) | 1979-04-23 | 1980-12-11 | Un procedimiento para la preparacion de derivados de la 7- amino-4-trifluormetilcumarina |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES497640A Expired ES8200675A1 (es) | 1979-04-23 | 1980-12-11 | Un procedimiento para la preparacion de derivados de la 7- amino-4-trifluormetilcumarina |
Country Status (8)
Country | Link |
---|---|
US (1) | US4294923A (es) |
EP (2) | EP0018112B1 (es) |
JP (1) | JPS56500494A (es) |
CA (1) | CA1152495A (es) |
DE (1) | DE3064684D1 (es) |
DK (1) | DK526280A (es) |
ES (2) | ES8104417A1 (es) |
WO (1) | WO1980002295A1 (es) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4409140A (en) * | 1979-04-23 | 1983-10-11 | Smith Robert E | Substrates and method for determining enzymes |
DE2936543A1 (de) * | 1979-09-10 | 1981-04-09 | Behringwerke Ag, 3550 Marburg | Chromogene verbindungen |
FR2497798A1 (fr) * | 1981-01-09 | 1982-07-16 | Pharmindustrie | Nouveaux peptides portant un fluorophore, procede pour leur preparation et leur application au dosage fluorimetrique des endotoxines |
US4388233A (en) * | 1981-05-15 | 1983-06-14 | The Regents Of The University Of California | Synthetic substrates for enzyme analysis |
US4451563A (en) * | 1981-08-28 | 1984-05-29 | Kaufman Richard A | Method for increasing the sensitivity of assays |
JPS59112952A (ja) * | 1982-12-21 | 1984-06-29 | Taisho Pharmaceut Co Ltd | ペプチド誘導体 |
US4874695A (en) * | 1983-03-08 | 1989-10-17 | American Home Products Corp. | Rapid indentification of yeast and other fungal microorganisms by enzyme detection |
US4803162A (en) * | 1984-05-15 | 1989-02-07 | Fluorodiagnostic Limited Partners | Composition, article and process for detecting a microorganism |
US4965193A (en) * | 1984-08-06 | 1990-10-23 | Washington Research Foundation | Detection of microbial beta-lactamase |
US4740459A (en) * | 1984-08-06 | 1988-04-26 | Washington Research Foundation | Fluorescence assay for microbial beta-lactamase |
JPS61108400A (ja) * | 1984-10-31 | 1986-05-27 | Amano Pharmaceut Co Ltd | コレステロ−ルの定量法 |
CA1293591C (en) * | 1985-01-11 | 1991-12-24 | Charles A. Kettner | Peptide substrates for detecting virus-specified protease activity |
US4801534A (en) * | 1985-05-28 | 1989-01-31 | Coulter Electronics, Inc. | Water soluble zanthylium derivative substrates |
US4694070A (en) * | 1985-05-28 | 1987-09-15 | Coulter Electronics, Inc. | Water soluble xanthylium derivatives substrates |
JPS62126196A (ja) * | 1985-11-26 | 1987-06-08 | Nitto Boseki Co Ltd | 新規なα1−アンチトリプシン測定用化合物 |
US4812409A (en) * | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Hydrolyzable fluorescent substrates and analytical determinations using same |
DE3614647A1 (de) * | 1986-04-30 | 1987-11-05 | Euratom | 7-phenylessigsaeure-4-alkyl-coumarinylamide, verfahren zu ihrer herstellung sowie ihre verwendung in verfahren zur fluorometrischen bestimmung der aktivitaet von hydrolasen, insbesondere von penicillin-g-acylase |
EP0255341B1 (en) * | 1986-07-29 | 1993-02-03 | Sunstar Kabushiki Kaisha | Reagent for testing periodontal diseases |
US4994376A (en) * | 1987-05-27 | 1991-02-19 | The Research Foundation Of State University Of Ny | Detection of bacteroides gingivalis |
AU652603B2 (en) * | 1990-12-28 | 1994-09-01 | Dade International Inc. | Method and composition for determining antimicrobial susceptibility of the majority of clinically significant gram positive organisms |
FR2677023B1 (fr) * | 1991-05-30 | 1994-02-25 | Eurobio Laboratoires | Derives de coumarine hydrosolubles, leur preparation et leur utilisation comme substrat d'enzyme. |
FR2703683B1 (fr) * | 1993-04-08 | 1995-07-07 | Seractec | Dérivés d'aminocoumarines hydrophobes, et leur utilisation comme substrats d'enzymes protéolytiques ou pour la préparation de tels substrats. |
GB9525403D0 (en) * | 1995-12-13 | 1996-02-14 | Univ Sunderland | Method for monitoring enzymes |
DE102007017681A1 (de) * | 2007-04-14 | 2009-01-08 | Papst Licensing Gmbh & Co. Kg | Vorrichtung und Verfahren zum Messen der Aktivität von Enzymen nach Inhibitorentzug |
CN102426157B (zh) * | 2011-11-16 | 2013-02-27 | 江南大学 | 一种基于功能化金纳米颗粒的酪氨酸酶活性分析方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4137225A (en) * | 1975-07-11 | 1979-01-30 | Ab Kabi | Novel chromogenic enzyme substrates |
US4056519A (en) * | 1976-05-10 | 1977-11-01 | Eli Lilly And Company | Substrate for assay of plasmin |
US4167449A (en) * | 1976-07-29 | 1979-09-11 | American Hospital Supply Corporation | Composition and method for determining transferase and protease activity |
US4147692A (en) * | 1977-02-26 | 1979-04-03 | Ajinomoto Co., Inc. | Novel dipeptide derivatives, and method of measuring enzymatic activity |
JPS53105477A (en) * | 1977-02-26 | 1978-09-13 | Ajinomoto Co Inc | 7-glycylprolylamono-4-methylcoumarine |
US4191809A (en) * | 1977-02-26 | 1980-03-04 | Ajinomoto Co., Inc. | Method of measuring enzymatic activity using novel peptide derivatives |
US4215047A (en) * | 1977-06-06 | 1980-07-29 | Ajinomoto Company Incorporated | 7-(Arginylamino)-4-methylcoumarins |
US4155916A (en) * | 1977-08-29 | 1979-05-22 | Dolbeare Frank A | Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes |
US4229528A (en) * | 1978-11-13 | 1980-10-21 | Smith Robert E | Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes |
-
1979
- 1979-04-23 US US06/032,444 patent/US4294923A/en not_active Expired - Lifetime
-
1980
- 1980-03-27 EP EP80300960A patent/EP0018112B1/en not_active Expired
- 1980-04-09 CA CA000349448A patent/CA1152495A/en not_active Expired
- 1980-04-16 JP JP50105180A patent/JPS56500494A/ja active Pending
- 1980-04-16 WO PCT/US1980/000430 patent/WO1980002295A1/en active IP Right Grant
- 1980-04-16 DE DE8080900871T patent/DE3064684D1/de not_active Expired
- 1980-04-22 ES ES490788A patent/ES8104417A1/es not_active Expired
- 1980-11-04 EP EP80900871A patent/EP0034586B1/en not_active Expired
- 1980-12-10 DK DK526280A patent/DK526280A/da not_active Application Discontinuation
- 1980-12-11 ES ES497640A patent/ES8200675A1/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
WO1980002295A1 (en) | 1980-10-30 |
EP0034586A4 (en) | 1981-05-15 |
EP0034586B1 (en) | 1983-08-31 |
ES497640A0 (es) | 1981-11-16 |
EP0018112A1 (en) | 1980-10-29 |
CA1152495A (en) | 1983-08-23 |
ES8200675A1 (es) | 1981-11-16 |
ES490788A0 (es) | 1981-04-16 |
DK526280A (da) | 1980-12-10 |
DE3064684D1 (en) | 1983-10-06 |
JPS56500494A (es) | 1981-04-16 |
US4294923A (en) | 1981-10-13 |
EP0034586A1 (en) | 1981-09-02 |
EP0018112B1 (en) | 1983-07-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19990601 |