ES2076153T3 - Substratos cromogenos. - Google Patents

Substratos cromogenos.

Info

Publication number
ES2076153T3
ES2076153T3 ES88115957T ES88115957T ES2076153T3 ES 2076153 T3 ES2076153 T3 ES 2076153T3 ES 88115957 T ES88115957 T ES 88115957T ES 88115957 T ES88115957 T ES 88115957T ES 2076153 T3 ES2076153 T3 ES 2076153T3
Authority
ES
Spain
Prior art keywords
chromogenic substrates
detection
range
enzymatic hydrolysis
hydrolyzing enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88115957T
Other languages
English (en)
Inventor
Klaus Dr Habenstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2076153T3 publication Critical patent/ES2076153T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/802—Chromogenic or luminescent peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Particle Formation And Scattering Control In Inkjet Printers (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

SUSTRATOS CROMOGENOS PARA LA VERIFICACION DE ENZIMAS HIDROLIZANTES, PROCESO PARA LA FABRICACION DE ESTOS SUSTRATOS CROMOGENOS Y USO DE LOS MISMOS. LA CUANTIFICACION DE LOS ENZIMAS HIDROLIZANTES CON FINES DIAGNOSTICOS SE HACE HASTA AHORA MEDIANTE LA DETERMINACION DE LAS CANTIDADES DE UN COLORANTE FLUORESCENTE O CROMOGENO FUERTEMENTE ABSORBENTE LIBERADAS EN LA REACCION HIDROLITICA, CUYA COMPROBACION SOLO PODIA HACERSE DE FORMA EXCLUSIVAMENTE INSTRUMENTAL EN PARTE O EN ZONAS DE PH NEUTRAL O ALCALINO. LOS SUSTRATOS CROMOGENOS DE LA PATENTE LLEVAN TRAS LA HIDROLISIS ENZIMATICA, INDEPENDIENTEMENTE DEL PH, A SEÑALES DE ALTA SENSIBILIDAD MEDIBLES ESPECIFICAMENTE. SE PROPONEN COMPUESTOS DE COLORANTES AZOICOS CON LA FORMULA GENERAL A - N = N - B (OR) DONDE A) Y B) TIENEN LA SIGNIFICACION CITADA EN LA SOLICITUD Y R) ES UN RESTO LIBERABLE MEDIANTE HIDROLISIS ENZIMATICA, CON LA EXCEPCION DEL RESTO CARBONILO. TRAS LA HIDROLISIS ENZIMATICA SE CONSIGUEN, INDEPENDIENTEMENTE DEL PH, SEÑALES DE ALTA SENSIBILIDAD ESPECIFICAMENTE MEDIBLES. LOS COMPUESTOS DE COLORANTES AZOICOS NOMBRADOS SIRVEN PARA LA VERIFICACION DE ENZIMAS HIDROLIZANTES, EN ESPECIAL DE GLUCOSIDASAS, FOSFATASAS Y SULFATASAS.
ES88115957T 1987-09-30 1988-09-28 Substratos cromogenos. Expired - Lifetime ES2076153T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873732871 DE3732871A1 (de) 1987-09-30 1987-09-30 Chromogene substrate

Publications (1)

Publication Number Publication Date
ES2076153T3 true ES2076153T3 (es) 1995-11-01

Family

ID=6337166

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88115957T Expired - Lifetime ES2076153T3 (es) 1987-09-30 1988-09-28 Substratos cromogenos.

Country Status (8)

Country Link
US (1) US5077200A (es)
EP (1) EP0310014B1 (es)
JP (1) JP2675835B2 (es)
AT (1) ATE124089T1 (es)
AU (1) AU608900B2 (es)
CA (1) CA1332806C (es)
DE (2) DE3732871A1 (es)
ES (1) ES2076153T3 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8801702D0 (sv) * 1988-05-05 1988-05-05 Pharmacia Ab Sett vid bestemning av enzymatisk aktivitet
DE3916729A1 (de) * 1989-05-23 1990-12-06 Behringwerke Ag Fluorogene verbindungen und ihre verwendung
GB9020364D0 (en) * 1990-09-18 1990-10-31 Cambridge Consultants Time temperature indicator
DE4406665A1 (de) * 1994-03-01 1995-09-07 Biosynth Ag Enzymsubstrate mit 2-(2-Thiazolylazo)-p-kresol als chromogener Gruppe
JPH11514849A (ja) * 1995-07-12 1999-12-21 チャーム サイエンシズ インコーポレイテッド 検査サンプルを検出するための検査装置、システム及び方法
US5827675A (en) * 1995-07-12 1998-10-27 Charm Sciences, Inc. Test apparatus, system and method for the detection of test samples
US6770468B1 (en) 1999-09-14 2004-08-03 Genzyme Glycobiology Research Institute, Inc. Phosphodiester-α-GlcNAcase of the lysosomal targeting pathway
US6537785B1 (en) 1999-09-14 2003-03-25 Genzyme Glycobiology Research Institute, Inc. Methods of treating lysosomal storage diseases
FR2824141B1 (fr) * 2001-04-25 2005-12-23 Proteus Methode de detection de la transformation d'un substrat par mesure d'un changement de pm, les produits mis en oeuvre et detectes selon ladite methode
TWI295681B (es) * 2001-12-19 2008-04-11 Ritek Corp
US6905856B2 (en) 2001-12-21 2005-06-14 Genzyme Glycobiology Research Institute, Inc. Soluble GlcNAc phosphotransferase
US6800472B2 (en) 2001-12-21 2004-10-05 Genzyme Glycobiology Research Institute, Inc. Expression of lysosomal hydrolase in cells expressing pro-N-acetylglucosamine-1-phosphodiester α-N-acetyl glucosimanidase
JP2014530208A (ja) * 2011-09-28 2014-11-17 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト アゾメディエーター

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH333924A (de) * 1953-10-30 1958-11-15 Ici Ltd Verfahren zur Herstellung von Azofarbstoffen
JPS6058746B2 (ja) * 1977-09-22 1985-12-21 中外製薬株式会社 高級脂肪酸エステル
DE2836644A1 (de) * 1978-08-22 1980-03-06 Boehringer Mannheim Gmbh Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten und dafuer geeignete chromogene
DE2905531A1 (de) * 1979-02-14 1981-01-08 Boehringer Mannheim Gmbh Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten
JPS57159499A (en) * 1981-03-26 1982-10-01 Fuji Photo Film Co Ltd Substrate for determining alkaline phosphatase and determining method
DE3411574A1 (de) * 1984-03-29 1985-10-10 Boehringer Mannheim Gmbh, 6800 Mannheim Glycoside von resorufin-derivaten, verfahren zu deren herstellung sowie deren verwendung zur bestimmung der aktivitaet von glycosidasen
DE3412939A1 (de) * 1984-04-06 1985-10-17 Behringwerke Ag, 3550 Marburg Substrate fuer hydrolasen, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
US5077200A (en) 1991-12-31
JPH01115962A (ja) 1989-05-09
EP0310014B1 (de) 1995-06-21
DE3854032D1 (de) 1995-07-27
EP0310014A2 (de) 1989-04-05
DE3732871A1 (de) 1989-04-20
EP0310014A3 (de) 1991-03-06
CA1332806C (en) 1994-11-01
AU608900B2 (en) 1991-04-18
JP2675835B2 (ja) 1997-11-12
ATE124089T1 (de) 1995-07-15
AU2295888A (en) 1989-04-06

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