ES2076153T3 - Substratos cromogenos. - Google Patents
Substratos cromogenos.Info
- Publication number
- ES2076153T3 ES2076153T3 ES88115957T ES88115957T ES2076153T3 ES 2076153 T3 ES2076153 T3 ES 2076153T3 ES 88115957 T ES88115957 T ES 88115957T ES 88115957 T ES88115957 T ES 88115957T ES 2076153 T3 ES2076153 T3 ES 2076153T3
- Authority
- ES
- Spain
- Prior art keywords
- chromogenic substrates
- detection
- range
- enzymatic hydrolysis
- hydrolyzing enzymes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
- C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/802—Chromogenic or luminescent peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Photoreceptors In Electrophotography (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Saccharide Compounds (AREA)
- Particle Formation And Scattering Control In Inkjet Printers (AREA)
Abstract
SUSTRATOS CROMOGENOS PARA LA VERIFICACION DE ENZIMAS HIDROLIZANTES, PROCESO PARA LA FABRICACION DE ESTOS SUSTRATOS CROMOGENOS Y USO DE LOS MISMOS. LA CUANTIFICACION DE LOS ENZIMAS HIDROLIZANTES CON FINES DIAGNOSTICOS SE HACE HASTA AHORA MEDIANTE LA DETERMINACION DE LAS CANTIDADES DE UN COLORANTE FLUORESCENTE O CROMOGENO FUERTEMENTE ABSORBENTE LIBERADAS EN LA REACCION HIDROLITICA, CUYA COMPROBACION SOLO PODIA HACERSE DE FORMA EXCLUSIVAMENTE INSTRUMENTAL EN PARTE O EN ZONAS DE PH NEUTRAL O ALCALINO. LOS SUSTRATOS CROMOGENOS DE LA PATENTE LLEVAN TRAS LA HIDROLISIS ENZIMATICA, INDEPENDIENTEMENTE DEL PH, A SEÑALES DE ALTA SENSIBILIDAD MEDIBLES ESPECIFICAMENTE. SE PROPONEN COMPUESTOS DE COLORANTES AZOICOS CON LA FORMULA GENERAL A - N = N - B (OR) DONDE A) Y B) TIENEN LA SIGNIFICACION CITADA EN LA SOLICITUD Y R) ES UN RESTO LIBERABLE MEDIANTE HIDROLISIS ENZIMATICA, CON LA EXCEPCION DEL RESTO CARBONILO. TRAS LA HIDROLISIS ENZIMATICA SE CONSIGUEN, INDEPENDIENTEMENTE DEL PH, SEÑALES DE ALTA SENSIBILIDAD ESPECIFICAMENTE MEDIBLES. LOS COMPUESTOS DE COLORANTES AZOICOS NOMBRADOS SIRVEN PARA LA VERIFICACION DE ENZIMAS HIDROLIZANTES, EN ESPECIAL DE GLUCOSIDASAS, FOSFATASAS Y SULFATASAS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19873732871 DE3732871A1 (de) | 1987-09-30 | 1987-09-30 | Chromogene substrate |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2076153T3 true ES2076153T3 (es) | 1995-11-01 |
Family
ID=6337166
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES88115957T Expired - Lifetime ES2076153T3 (es) | 1987-09-30 | 1988-09-28 | Substratos cromogenos. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5077200A (es) |
EP (1) | EP0310014B1 (es) |
JP (1) | JP2675835B2 (es) |
AT (1) | ATE124089T1 (es) |
AU (1) | AU608900B2 (es) |
CA (1) | CA1332806C (es) |
DE (2) | DE3732871A1 (es) |
ES (1) | ES2076153T3 (es) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE8801702D0 (sv) * | 1988-05-05 | 1988-05-05 | Pharmacia Ab | Sett vid bestemning av enzymatisk aktivitet |
DE3916729A1 (de) * | 1989-05-23 | 1990-12-06 | Behringwerke Ag | Fluorogene verbindungen und ihre verwendung |
GB9020364D0 (en) * | 1990-09-18 | 1990-10-31 | Cambridge Consultants | Time temperature indicator |
DE4406665A1 (de) * | 1994-03-01 | 1995-09-07 | Biosynth Ag | Enzymsubstrate mit 2-(2-Thiazolylazo)-p-kresol als chromogener Gruppe |
DE69629582T2 (de) * | 1995-07-12 | 2004-06-24 | Charm Sciences, Inc., Lawrence | Testvorrichtung, sytem und verfahren zum nachweis von testproben |
US5827675A (en) * | 1995-07-12 | 1998-10-27 | Charm Sciences, Inc. | Test apparatus, system and method for the detection of test samples |
US6642038B1 (en) | 1999-09-14 | 2003-11-04 | Genzyme Glycobiology Research Institute, Inc. | GlcNAc phosphotransferase of the lysosomal targeting pathway |
US6770468B1 (en) | 1999-09-14 | 2004-08-03 | Genzyme Glycobiology Research Institute, Inc. | Phosphodiester-α-GlcNAcase of the lysosomal targeting pathway |
FR2824141B1 (fr) * | 2001-04-25 | 2005-12-23 | Proteus | Methode de detection de la transformation d'un substrat par mesure d'un changement de pm, les produits mis en oeuvre et detectes selon ladite methode |
TWI295681B (es) * | 2001-12-19 | 2008-04-11 | Ritek Corp | |
US6800472B2 (en) | 2001-12-21 | 2004-10-05 | Genzyme Glycobiology Research Institute, Inc. | Expression of lysosomal hydrolase in cells expressing pro-N-acetylglucosamine-1-phosphodiester α-N-acetyl glucosimanidase |
US6905856B2 (en) | 2001-12-21 | 2005-06-14 | Genzyme Glycobiology Research Institute, Inc. | Soluble GlcNAc phosphotransferase |
CN103958463A (zh) * | 2011-09-28 | 2014-07-30 | 霍夫曼-拉罗奇有限公司 | 偶氮介体 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH333924A (de) * | 1953-10-30 | 1958-11-15 | Ici Ltd | Verfahren zur Herstellung von Azofarbstoffen |
JPS6058746B2 (ja) * | 1977-09-22 | 1985-12-21 | 中外製薬株式会社 | 高級脂肪酸エステル |
DE2836644A1 (de) * | 1978-08-22 | 1980-03-06 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten und dafuer geeignete chromogene |
DE2905531A1 (de) * | 1979-02-14 | 1981-01-08 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten |
JPS57159499A (en) * | 1981-03-26 | 1982-10-01 | Fuji Photo Film Co Ltd | Substrate for determining alkaline phosphatase and determining method |
DE3411574A1 (de) * | 1984-03-29 | 1985-10-10 | Boehringer Mannheim Gmbh, 6800 Mannheim | Glycoside von resorufin-derivaten, verfahren zu deren herstellung sowie deren verwendung zur bestimmung der aktivitaet von glycosidasen |
DE3412939A1 (de) * | 1984-04-06 | 1985-10-17 | Behringwerke Ag, 3550 Marburg | Substrate fuer hydrolasen, verfahren zu ihrer herstellung und ihre verwendung |
-
1987
- 1987-09-30 DE DE19873732871 patent/DE3732871A1/de not_active Withdrawn
-
1988
- 1988-09-28 EP EP88115957A patent/EP0310014B1/de not_active Expired - Lifetime
- 1988-09-28 ES ES88115957T patent/ES2076153T3/es not_active Expired - Lifetime
- 1988-09-28 DE DE3854032T patent/DE3854032D1/de not_active Expired - Fee Related
- 1988-09-28 US US07/251,166 patent/US5077200A/en not_active Expired - Lifetime
- 1988-09-28 AT AT88115957T patent/ATE124089T1/de not_active IP Right Cessation
- 1988-09-29 AU AU22958/88A patent/AU608900B2/en not_active Ceased
- 1988-09-29 CA CA000578824A patent/CA1332806C/en not_active Expired - Fee Related
- 1988-09-29 JP JP63242624A patent/JP2675835B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ATE124089T1 (de) | 1995-07-15 |
EP0310014B1 (de) | 1995-06-21 |
CA1332806C (en) | 1994-11-01 |
EP0310014A2 (de) | 1989-04-05 |
AU608900B2 (en) | 1991-04-18 |
AU2295888A (en) | 1989-04-06 |
JPH01115962A (ja) | 1989-05-09 |
JP2675835B2 (ja) | 1997-11-12 |
DE3854032D1 (de) | 1995-07-27 |
US5077200A (en) | 1991-12-31 |
DE3732871A1 (de) | 1989-04-20 |
EP0310014A3 (de) | 1991-03-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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