GB1128371A - Diagnostic composition - Google Patents

Diagnostic composition

Info

Publication number
GB1128371A
GB1128371A GB4205365A GB4205365A GB1128371A GB 1128371 A GB1128371 A GB 1128371A GB 4205365 A GB4205365 A GB 4205365A GB 4205365 A GB4205365 A GB 4205365A GB 1128371 A GB1128371 A GB 1128371A
Authority
GB
United Kingdom
Prior art keywords
bromo
ester
enzyme
diagnostic composition
pseudocholinesterase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4205365A
Inventor
Charles Edward Dalgliesh
Donald William Moss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Priority to GB4205365A priority Critical patent/GB1128371A/en
Publication of GB1128371A publication Critical patent/GB1128371A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/50Indoles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1,128,371. Diagnostic composition for hydrolytic enzymes. MILES LABORATORIES Inc. 4 Oct., 1965, No. 42053/65. Heading B1X. A diagnostic composition for detecting hydrolytic enzymes in body fluids comprises a bibulous carrier impregnated with an indoxyl or thioindoxyl ester. In the presence of a hydrolytic enzyme the ester is hydrolysed and a blue or red pigment of the indigo or indirubin type is formed by oxidation with the ambient air or an oxidising agent incorporated in the composition. The acid radical of the ester is selected having regard to the enzyme to be detected: acetate, butyrate, laurate or stearate for an esterase or lipase according to the length of the alkyl chain, phosphate (for phosphatases), sulphate (for sulphatases), glucuronide (for glucuronidases) or glycoside (for glycosidages). A suitable buffer may also be incorporated. By adding to the body fluid to be tested an inhibitor for one or more enzyme (e.g. eserine for pseudocholinesterase or NaF for pseudocholinesterase and aryl esterase), the relative proportions of various enzymes may be determined. Specified substituted indoxyls are the 5-bromo, 5-bromo-6-chloro, and 5-bromo-4 chloro-derivatives. Comparative details are given of alphanaphthyl acetate-Fast Blue B papers.
GB4205365A 1965-10-04 1965-10-04 Diagnostic composition Expired GB1128371A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB4205365A GB1128371A (en) 1965-10-04 1965-10-04 Diagnostic composition

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4205365A GB1128371A (en) 1965-10-04 1965-10-04 Diagnostic composition

Publications (1)

Publication Number Publication Date
GB1128371A true GB1128371A (en) 1968-09-25

Family

ID=10422625

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4205365A Expired GB1128371A (en) 1965-10-04 1965-10-04 Diagnostic composition

Country Status (1)

Country Link
GB (1) GB1128371A (en)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0007407A1 (en) * 1978-06-20 1980-02-06 Roche Diagnostics GmbH Diagnostic composition for the detection of leukocytes in body fluids, sulfophthalein esters as chromogens used for this composition, method of their preparation and their use for preparing diagnostic compositions
DE2854987A1 (en) 1978-12-20 1980-06-26 Boehringer Mannheim Gmbh DIAGNOSTIC AGENTS FOR DETECTING PROTEOLYTIC ENZYMS AND CHROMOGENS SUITABLE FOR THIS
EP0014929A1 (en) * 1979-02-14 1980-09-03 Roche Diagnostics GmbH Diagnostic means, process for its preparation and its use in determining leucocytes in body fluids
EP0025227A2 (en) * 1979-09-06 1981-03-18 Miles Laboratories, Inc. Precursor indicator compositions
EP0039880A1 (en) * 1980-05-09 1981-11-18 Roche Diagnostics GmbH Agents for the detection of esterolytic and/or proteolytic enzymes; and phenoxy-amino acids and phenoxy-peptide esters suitable as substrates therefor, process for their production and their use in producing the agents
EP0094554A1 (en) * 1982-05-17 1983-11-23 Miles Laboratories, Inc. Test for esterase activity in a liquid sample
US4723020A (en) * 1984-04-06 1988-02-02 Miles Laboratories, Inc. Chromogenic amino acid esters and peptide esters
EP0290217A2 (en) * 1987-05-07 1988-11-09 The Procter & Gamble Company An in vitro diagnostic method
EP0476930A1 (en) * 1990-09-12 1992-03-25 Sankyo Company Limited Enzyme assays
WO1996004400A1 (en) * 1994-08-04 1996-02-15 Medilite Diagnostika Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates
EP0698600A1 (en) 1994-08-22 1996-02-28 Bayer Corporation Composition, method and test device for determining the presence of leukocyte cells, esterase or protease in a test sample
EP0713093A2 (en) 1994-11-15 1996-05-22 Bayer Corporation Improved methodology for colorimetrically determining the concentration of white blood cells in a biological fluid
EP0848066A1 (en) * 1996-12-13 1998-06-17 MERCK PATENT GmbH Means and method for determining hydrolytic enzymes
EP1273283A1 (en) * 2001-07-06 2003-01-08 L'oreal Phosphated dye precursors and their application for dyeing keratinous fibres
FR2952817A1 (en) * 2009-11-23 2011-05-27 Oreal Composition, useful for dyeing keratin fibers, preferably human keratin fibers such as hair, comprises one or more dye precursors comprising indole derivatives and liquid organic compounds in a medium
EP1920067B1 (en) * 2005-08-31 2015-06-03 Kimberly-Clark Worldwide, Inc. Enzyme detection technique

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0007407A1 (en) * 1978-06-20 1980-02-06 Roche Diagnostics GmbH Diagnostic composition for the detection of leukocytes in body fluids, sulfophthalein esters as chromogens used for this composition, method of their preparation and their use for preparing diagnostic compositions
DE2854987A1 (en) 1978-12-20 1980-06-26 Boehringer Mannheim Gmbh DIAGNOSTIC AGENTS FOR DETECTING PROTEOLYTIC ENZYMS AND CHROMOGENS SUITABLE FOR THIS
EP0014929A1 (en) * 1979-02-14 1980-09-03 Roche Diagnostics GmbH Diagnostic means, process for its preparation and its use in determining leucocytes in body fluids
EP0025227A2 (en) * 1979-09-06 1981-03-18 Miles Laboratories, Inc. Precursor indicator compositions
EP0025227A3 (en) * 1979-09-06 1981-03-25 Miles Laboratories Inc. Precursor indicator compositions
EP0039880A1 (en) * 1980-05-09 1981-11-18 Roche Diagnostics GmbH Agents for the detection of esterolytic and/or proteolytic enzymes; and phenoxy-amino acids and phenoxy-peptide esters suitable as substrates therefor, process for their production and their use in producing the agents
US4551428A (en) * 1980-05-09 1985-11-05 Boehringer Mannheim Gmbh Agent for the determination of esterolytic and/or proteolytic enzymes
EP0094554A1 (en) * 1982-05-17 1983-11-23 Miles Laboratories, Inc. Test for esterase activity in a liquid sample
US4723020A (en) * 1984-04-06 1988-02-02 Miles Laboratories, Inc. Chromogenic amino acid esters and peptide esters
EP0290217A2 (en) * 1987-05-07 1988-11-09 The Procter & Gamble Company An in vitro diagnostic method
EP0290217A3 (en) * 1987-05-07 1989-01-25 The Procter & Gamble Company Diagnostic methods, products and kits for detecting periodontal diseases
US5051358A (en) * 1987-05-07 1991-09-24 The Procter & Gamble Company Diagnostic methods for detecting periodontal diseases
EP0476930A1 (en) * 1990-09-12 1992-03-25 Sankyo Company Limited Enzyme assays
WO1996004400A1 (en) * 1994-08-04 1996-02-15 Medilite Diagnostika Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates
US5589328A (en) * 1994-08-04 1996-12-31 Mahant; Vijay K. Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates
EP0698600A1 (en) 1994-08-22 1996-02-28 Bayer Corporation Composition, method and test device for determining the presence of leukocyte cells, esterase or protease in a test sample
EP0713093A2 (en) 1994-11-15 1996-05-22 Bayer Corporation Improved methodology for colorimetrically determining the concentration of white blood cells in a biological fluid
EP0848066A1 (en) * 1996-12-13 1998-06-17 MERCK PATENT GmbH Means and method for determining hydrolytic enzymes
DE19651886A1 (en) * 1996-12-13 1998-06-18 Merck Patent Gmbh Means and methods for the determination of hydrolytic enzymes
US6004769A (en) * 1996-12-13 1999-12-21 Merck Patent Gesellschaft Compositions and procedures for the determination of hydrolytic enzymes
EP1273283A1 (en) * 2001-07-06 2003-01-08 L'oreal Phosphated dye precursors and their application for dyeing keratinous fibres
FR2826867A1 (en) * 2001-07-06 2003-01-10 Oreal PHOSPHATE DYE PRECURSORS AND THEIR APPLICATION FOR DYING KERATINIC FIBERS
US6902586B2 (en) 2001-07-06 2005-06-07 L'oreal Phosphate dye precursors and use thereof for dyeing keratin fibers
EP1920067B1 (en) * 2005-08-31 2015-06-03 Kimberly-Clark Worldwide, Inc. Enzyme detection technique
FR2952817A1 (en) * 2009-11-23 2011-05-27 Oreal Composition, useful for dyeing keratin fibers, preferably human keratin fibers such as hair, comprises one or more dye precursors comprising indole derivatives and liquid organic compounds in a medium

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