GB1128371A - Diagnostic composition - Google Patents
Diagnostic compositionInfo
- Publication number
- GB1128371A GB1128371A GB4205365A GB4205365A GB1128371A GB 1128371 A GB1128371 A GB 1128371A GB 4205365 A GB4205365 A GB 4205365A GB 4205365 A GB4205365 A GB 4205365A GB 1128371 A GB1128371 A GB 1128371A
- Authority
- GB
- United Kingdom
- Prior art keywords
- bromo
- ester
- enzyme
- diagnostic composition
- pseudocholinesterase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/50—Indoles
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1,128,371. Diagnostic composition for hydrolytic enzymes. MILES LABORATORIES Inc. 4 Oct., 1965, No. 42053/65. Heading B1X. A diagnostic composition for detecting hydrolytic enzymes in body fluids comprises a bibulous carrier impregnated with an indoxyl or thioindoxyl ester. In the presence of a hydrolytic enzyme the ester is hydrolysed and a blue or red pigment of the indigo or indirubin type is formed by oxidation with the ambient air or an oxidising agent incorporated in the composition. The acid radical of the ester is selected having regard to the enzyme to be detected: acetate, butyrate, laurate or stearate for an esterase or lipase according to the length of the alkyl chain, phosphate (for phosphatases), sulphate (for sulphatases), glucuronide (for glucuronidases) or glycoside (for glycosidages). A suitable buffer may also be incorporated. By adding to the body fluid to be tested an inhibitor for one or more enzyme (e.g. eserine for pseudocholinesterase or NaF for pseudocholinesterase and aryl esterase), the relative proportions of various enzymes may be determined. Specified substituted indoxyls are the 5-bromo, 5-bromo-6-chloro, and 5-bromo-4 chloro-derivatives. Comparative details are given of alphanaphthyl acetate-Fast Blue B papers.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB4205365A GB1128371A (en) | 1965-10-04 | 1965-10-04 | Diagnostic composition |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB4205365A GB1128371A (en) | 1965-10-04 | 1965-10-04 | Diagnostic composition |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1128371A true GB1128371A (en) | 1968-09-25 |
Family
ID=10422625
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB4205365A Expired GB1128371A (en) | 1965-10-04 | 1965-10-04 | Diagnostic composition |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1128371A (en) |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0007407A1 (en) * | 1978-06-20 | 1980-02-06 | Roche Diagnostics GmbH | Diagnostic composition for the detection of leukocytes in body fluids, sulfophthalein esters as chromogens used for this composition, method of their preparation and their use for preparing diagnostic compositions |
DE2854987A1 (en) | 1978-12-20 | 1980-06-26 | Boehringer Mannheim Gmbh | DIAGNOSTIC AGENTS FOR DETECTING PROTEOLYTIC ENZYMS AND CHROMOGENS SUITABLE FOR THIS |
EP0014929A1 (en) * | 1979-02-14 | 1980-09-03 | Roche Diagnostics GmbH | Diagnostic means, process for its preparation and its use in determining leucocytes in body fluids |
EP0025227A2 (en) * | 1979-09-06 | 1981-03-18 | Miles Laboratories, Inc. | Precursor indicator compositions |
EP0039880A1 (en) * | 1980-05-09 | 1981-11-18 | Roche Diagnostics GmbH | Agents for the detection of esterolytic and/or proteolytic enzymes; and phenoxy-amino acids and phenoxy-peptide esters suitable as substrates therefor, process for their production and their use in producing the agents |
EP0094554A1 (en) * | 1982-05-17 | 1983-11-23 | Miles Laboratories, Inc. | Test for esterase activity in a liquid sample |
US4723020A (en) * | 1984-04-06 | 1988-02-02 | Miles Laboratories, Inc. | Chromogenic amino acid esters and peptide esters |
EP0290217A2 (en) * | 1987-05-07 | 1988-11-09 | The Procter & Gamble Company | An in vitro diagnostic method |
EP0476930A1 (en) * | 1990-09-12 | 1992-03-25 | Sankyo Company Limited | Enzyme assays |
WO1996004400A1 (en) * | 1994-08-04 | 1996-02-15 | Medilite Diagnostika | Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates |
EP0698600A1 (en) | 1994-08-22 | 1996-02-28 | Bayer Corporation | Composition, method and test device for determining the presence of leukocyte cells, esterase or protease in a test sample |
EP0713093A2 (en) | 1994-11-15 | 1996-05-22 | Bayer Corporation | Improved methodology for colorimetrically determining the concentration of white blood cells in a biological fluid |
EP0848066A1 (en) * | 1996-12-13 | 1998-06-17 | MERCK PATENT GmbH | Means and method for determining hydrolytic enzymes |
EP1273283A1 (en) * | 2001-07-06 | 2003-01-08 | L'oreal | Phosphated dye precursors and their application for dyeing keratinous fibres |
FR2952817A1 (en) * | 2009-11-23 | 2011-05-27 | Oreal | Composition, useful for dyeing keratin fibers, preferably human keratin fibers such as hair, comprises one or more dye precursors comprising indole derivatives and liquid organic compounds in a medium |
EP1920067B1 (en) * | 2005-08-31 | 2015-06-03 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
-
1965
- 1965-10-04 GB GB4205365A patent/GB1128371A/en not_active Expired
Cited By (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0007407A1 (en) * | 1978-06-20 | 1980-02-06 | Roche Diagnostics GmbH | Diagnostic composition for the detection of leukocytes in body fluids, sulfophthalein esters as chromogens used for this composition, method of their preparation and their use for preparing diagnostic compositions |
DE2854987A1 (en) | 1978-12-20 | 1980-06-26 | Boehringer Mannheim Gmbh | DIAGNOSTIC AGENTS FOR DETECTING PROTEOLYTIC ENZYMS AND CHROMOGENS SUITABLE FOR THIS |
EP0014929A1 (en) * | 1979-02-14 | 1980-09-03 | Roche Diagnostics GmbH | Diagnostic means, process for its preparation and its use in determining leucocytes in body fluids |
EP0025227A2 (en) * | 1979-09-06 | 1981-03-18 | Miles Laboratories, Inc. | Precursor indicator compositions |
EP0025227A3 (en) * | 1979-09-06 | 1981-03-25 | Miles Laboratories Inc. | Precursor indicator compositions |
EP0039880A1 (en) * | 1980-05-09 | 1981-11-18 | Roche Diagnostics GmbH | Agents for the detection of esterolytic and/or proteolytic enzymes; and phenoxy-amino acids and phenoxy-peptide esters suitable as substrates therefor, process for their production and their use in producing the agents |
US4551428A (en) * | 1980-05-09 | 1985-11-05 | Boehringer Mannheim Gmbh | Agent for the determination of esterolytic and/or proteolytic enzymes |
EP0094554A1 (en) * | 1982-05-17 | 1983-11-23 | Miles Laboratories, Inc. | Test for esterase activity in a liquid sample |
US4723020A (en) * | 1984-04-06 | 1988-02-02 | Miles Laboratories, Inc. | Chromogenic amino acid esters and peptide esters |
EP0290217A2 (en) * | 1987-05-07 | 1988-11-09 | The Procter & Gamble Company | An in vitro diagnostic method |
EP0290217A3 (en) * | 1987-05-07 | 1989-01-25 | The Procter & Gamble Company | Diagnostic methods, products and kits for detecting periodontal diseases |
US5051358A (en) * | 1987-05-07 | 1991-09-24 | The Procter & Gamble Company | Diagnostic methods for detecting periodontal diseases |
EP0476930A1 (en) * | 1990-09-12 | 1992-03-25 | Sankyo Company Limited | Enzyme assays |
WO1996004400A1 (en) * | 1994-08-04 | 1996-02-15 | Medilite Diagnostika | Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates |
US5589328A (en) * | 1994-08-04 | 1996-12-31 | Mahant; Vijay K. | Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates |
EP0698600A1 (en) | 1994-08-22 | 1996-02-28 | Bayer Corporation | Composition, method and test device for determining the presence of leukocyte cells, esterase or protease in a test sample |
EP0713093A2 (en) | 1994-11-15 | 1996-05-22 | Bayer Corporation | Improved methodology for colorimetrically determining the concentration of white blood cells in a biological fluid |
EP0848066A1 (en) * | 1996-12-13 | 1998-06-17 | MERCK PATENT GmbH | Means and method for determining hydrolytic enzymes |
DE19651886A1 (en) * | 1996-12-13 | 1998-06-18 | Merck Patent Gmbh | Means and methods for the determination of hydrolytic enzymes |
US6004769A (en) * | 1996-12-13 | 1999-12-21 | Merck Patent Gesellschaft | Compositions and procedures for the determination of hydrolytic enzymes |
EP1273283A1 (en) * | 2001-07-06 | 2003-01-08 | L'oreal | Phosphated dye precursors and their application for dyeing keratinous fibres |
FR2826867A1 (en) * | 2001-07-06 | 2003-01-10 | Oreal | PHOSPHATE DYE PRECURSORS AND THEIR APPLICATION FOR DYING KERATINIC FIBERS |
US6902586B2 (en) | 2001-07-06 | 2005-06-07 | L'oreal | Phosphate dye precursors and use thereof for dyeing keratin fibers |
EP1920067B1 (en) * | 2005-08-31 | 2015-06-03 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
FR2952817A1 (en) * | 2009-11-23 | 2011-05-27 | Oreal | Composition, useful for dyeing keratin fibers, preferably human keratin fibers such as hair, comprises one or more dye precursors comprising indole derivatives and liquid organic compounds in a medium |
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