GB1263202A - Alkaline phosphatase test - Google Patents
Alkaline phosphatase testInfo
- Publication number
- GB1263202A GB1263202A GB1831070A GB1831070A GB1263202A GB 1263202 A GB1263202 A GB 1263202A GB 1831070 A GB1831070 A GB 1831070A GB 1831070 A GB1831070 A GB 1831070A GB 1263202 A GB1263202 A GB 1263202A
- Authority
- GB
- United Kingdom
- Prior art keywords
- alkaline phosphatase
- solution
- test
- incubated
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/90—Developer
- C12Q2326/96—4-Amino-antipyrine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1,263,202. Determination of alkaline phosphatase. PFIZER Inc. 16 April, 1970 [14 Jan., 1970], No. 18310/70. Heading B1X. A test pack for the determination of alkaline phosphatase in human serum or plasma comprises bottled reagents comprising (A), a 3M aqueous solution of 2-amino-2-methyl-1-propanol containing about 1% w/v disodium monophenyl phosphate and about 0.6% w/v 4- aminoantipyrine, having a pH of about 10; (B), a buffered aqueous solution pH about 10 and containing about 0.32% w/v sodium carbonate and about 0.17% w/v sodium bicarbonate; (C), a colour developer solution having pH about 10. and containing about 0.32% w/v sodium carbonate, about 0.17% w/v sodium bicarbonate and about 2.4% w/v potassium ferricyanide, and (D) a standard solution containing 0.1% w/v phenol in 0.1 NHCI. In carrying out the test, sample is incubated with a mixture of solutions (A) and B, the resulting solution is incubated with reagent (C), and the product is measured at 500 mu against a standard prepared by incubating a mixture of solutions C and D.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US293670A | 1970-01-14 | 1970-01-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1263202A true GB1263202A (en) | 1972-02-09 |
Family
ID=21703271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1831070A Expired GB1263202A (en) | 1970-01-14 | 1970-04-16 | Alkaline phosphatase test |
Country Status (2)
| Country | Link |
|---|---|
| DE (1) | DE2059278A1 (en) |
| GB (1) | GB1263202A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111220609A (en) * | 2020-02-05 | 2020-06-02 | 江苏大学 | Based on CeVO4Colorimetric detection method of alkaline phosphatase Activity |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH625833A5 (en) * | 1975-12-24 | 1981-10-15 | Hoffmann La Roche | Method for the determination of acid phosphatase |
| US4206280A (en) | 1975-12-24 | 1980-06-03 | Hoffmann-La Roche Inc. | Determination of acid phosphatase |
-
1970
- 1970-04-16 GB GB1831070A patent/GB1263202A/en not_active Expired
- 1970-12-02 DE DE19702059278 patent/DE2059278A1/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111220609A (en) * | 2020-02-05 | 2020-06-02 | 江苏大学 | Based on CeVO4Colorimetric detection method of alkaline phosphatase Activity |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2059278A1 (en) | 1971-07-22 |
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