JPS5599198A - Determination of sero-enzyme by p-nitroanilide substrate - Google Patents
Determination of sero-enzyme by p-nitroanilide substrateInfo
- Publication number
- JPS5599198A JPS5599198A JP722079A JP722079A JPS5599198A JP S5599198 A JPS5599198 A JP S5599198A JP 722079 A JP722079 A JP 722079A JP 722079 A JP722079 A JP 722079A JP S5599198 A JPS5599198 A JP S5599198A
- Authority
- JP
- Japan
- Prior art keywords
- sero
- enzyme
- substrate
- determination
- coloring matter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000758 substrate Substances 0.000 title abstract 3
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 abstract 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 abstract 2
- 238000004040 coloring Methods 0.000 abstract 2
- YQUVCSBJEUQKSH-UHFFFAOYSA-N protochatechuic acid Natural products OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 abstract 2
- WKOLLVMJNQIZCI-UHFFFAOYSA-N vanillic acid Chemical compound COC1=CC(C(O)=O)=CC=C1O WKOLLVMJNQIZCI-UHFFFAOYSA-N 0.000 abstract 2
- TUUBOHWZSQXCSW-UHFFFAOYSA-N vanillic acid Natural products COC1=CC(O)=CC(C(O)=O)=C1 TUUBOHWZSQXCSW-UHFFFAOYSA-N 0.000 abstract 2
- ICMFHHGKLRTCBM-UHFFFAOYSA-N 4-nitrobenzenediazonium Chemical class [O-][N+](=O)C1=CC=C([N+]#N)C=C1 ICMFHHGKLRTCBM-UHFFFAOYSA-N 0.000 abstract 1
- AXZJHDNQDSVIDR-NSHDSACASA-N 4178-93-2 Chemical compound CC(C)C[C@H](N)C(=O)NC1=CC=C([N+]([O-])=O)C=C1 AXZJHDNQDSVIDR-NSHDSACASA-N 0.000 abstract 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 238000004737 colorimetric analysis Methods 0.000 abstract 1
- -1 sodium carbonate Chemical compound 0.000 abstract 1
- 229910000029 sodium carbonate Inorganic materials 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To determine the sero-enzyme with a good reproduciblity, free from variation in color with temperatur and time, by reaction of a diazotized p-nitroaniline liberated from the substrate with vanillic acid, and by colorimitry of the formed coloring matter.
CONSTITUTION: p-Nitroaniline liberated by using a substrate, e.g. L-leucyl p-nitroanilide, according to sero-enzyme concentration, is reacted with a nitrite under acidic conditions, to give a p-nitrobenzene-diazonium salt, which is coupled with vanillic acid in the presence of an alkali carbonate, e.g. sodium carbonate, to form an azo coloring matter (C). The colorimetry of C provides the sero-enzyme value.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP722079A JPS5599198A (en) | 1979-01-26 | 1979-01-26 | Determination of sero-enzyme by p-nitroanilide substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP722079A JPS5599198A (en) | 1979-01-26 | 1979-01-26 | Determination of sero-enzyme by p-nitroanilide substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5599198A true JPS5599198A (en) | 1980-07-28 |
Family
ID=11659906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP722079A Pending JPS5599198A (en) | 1979-01-26 | 1979-01-26 | Determination of sero-enzyme by p-nitroanilide substrate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5599198A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4414327A (en) * | 1980-12-02 | 1983-11-08 | Public Health Laboratory Service Board | Method for the estimation of N-acylated primary aromatic amines |
-
1979
- 1979-01-26 JP JP722079A patent/JPS5599198A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4414327A (en) * | 1980-12-02 | 1983-11-08 | Public Health Laboratory Service Board | Method for the estimation of N-acylated primary aromatic amines |
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