JPS56113299A - Method and reagent for determining leucine aminopeptidase activity - Google Patents

Method and reagent for determining leucine aminopeptidase activity

Info

Publication number
JPS56113299A
JPS56113299A JP1602680A JP1602680A JPS56113299A JP S56113299 A JPS56113299 A JP S56113299A JP 1602680 A JP1602680 A JP 1602680A JP 1602680 A JP1602680 A JP 1602680A JP S56113299 A JPS56113299 A JP S56113299A
Authority
JP
Japan
Prior art keywords
lap
substrate
leucine aminopeptidase
activity
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1602680A
Other languages
Japanese (ja)
Other versions
JPS6261319B2 (en
Inventor
Tetsuyoshi Yokojima
Yoshinori Matsumura
Fumiaki Tsuyukuchi
Shigeo Kondo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daiichi Kagaku Yakuhin Co Ltd
Daiichi Pure Chemicals Co Ltd
Original Assignee
Daiichi Kagaku Yakuhin Co Ltd
Daiichi Pure Chemicals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Kagaku Yakuhin Co Ltd, Daiichi Pure Chemicals Co Ltd filed Critical Daiichi Kagaku Yakuhin Co Ltd
Priority to JP1602680A priority Critical patent/JPS56113299A/en
Publication of JPS56113299A publication Critical patent/JPS56113299A/en
Publication of JPS6261319B2 publication Critical patent/JPS6261319B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the activity of leucine aminopeptidase (LAP) in a biospecimen, rapidly, easily and accurately, by using a L-leucyl-4-N,N-dialkylaminoanilide derivative which is a novel substrate, or its salt.
CONSTITUTION: A novel substrate of formula (R1 and R2 are lower alkyl), e.g. L-leucyl-3-carboxy-4-N,N-dimethylaminoanilide, is converted by the enzymatic action of LAP to 2-dialkylamino-5-aminobenzoic acid, which is converted to a colored compound by the oxidative condensation with a proper coupler and subjected to colorimetry to determine the LAP activity. High solubility of the substrate in water enables easy preparation of the substrate solution. The reproducibility of the determination is good because of small variation of the color with temperature and time.
COPYRIGHT: (C)1981,JPO&Japio
JP1602680A 1980-02-14 1980-02-14 Method and reagent for determining leucine aminopeptidase activity Granted JPS56113299A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1602680A JPS56113299A (en) 1980-02-14 1980-02-14 Method and reagent for determining leucine aminopeptidase activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1602680A JPS56113299A (en) 1980-02-14 1980-02-14 Method and reagent for determining leucine aminopeptidase activity

Publications (2)

Publication Number Publication Date
JPS56113299A true JPS56113299A (en) 1981-09-07
JPS6261319B2 JPS6261319B2 (en) 1987-12-21

Family

ID=11905047

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1602680A Granted JPS56113299A (en) 1980-02-14 1980-02-14 Method and reagent for determining leucine aminopeptidase activity

Country Status (1)

Country Link
JP (1) JPS56113299A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5963198A (en) * 1982-10-01 1984-04-10 Toyo Jozo Co Ltd Quantitative assay using enzyme
JPS5988099A (en) * 1982-11-15 1984-05-21 Toyo Jozo Co Ltd Novel measurement of enzymatic activity

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5963198A (en) * 1982-10-01 1984-04-10 Toyo Jozo Co Ltd Quantitative assay using enzyme
JPH0358720B2 (en) * 1982-10-01 1991-09-06 Toyo Jozo Kk
JPS5988099A (en) * 1982-11-15 1984-05-21 Toyo Jozo Co Ltd Novel measurement of enzymatic activity
JPS6210160B2 (en) * 1982-11-15 1987-03-04 Toyo Jozo Kk

Also Published As

Publication number Publication date
JPS6261319B2 (en) 1987-12-21

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