JPS56113299A - Method and reagent for determining leucine aminopeptidase activity - Google Patents
Method and reagent for determining leucine aminopeptidase activityInfo
- Publication number
- JPS56113299A JPS56113299A JP1602680A JP1602680A JPS56113299A JP S56113299 A JPS56113299 A JP S56113299A JP 1602680 A JP1602680 A JP 1602680A JP 1602680 A JP1602680 A JP 1602680A JP S56113299 A JPS56113299 A JP S56113299A
- Authority
- JP
- Japan
- Prior art keywords
- lap
- substrate
- leucine aminopeptidase
- activity
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000002704 Leucyl aminopeptidase Human genes 0.000 title abstract 5
- 108010004098 Leucyl aminopeptidase Proteins 0.000 title abstract 5
- 230000000694 effects Effects 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 239000000758 substrate Substances 0.000 abstract 4
- 125000000217 alkyl group Chemical group 0.000 abstract 1
- 229960004050 aminobenzoic acid Drugs 0.000 abstract 1
- 238000004737 colorimetric analysis Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000009833 condensation Methods 0.000 abstract 1
- 230000005494 condensation Effects 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To determine the activity of leucine aminopeptidase (LAP) in a biospecimen, rapidly, easily and accurately, by using a L-leucyl-4-N,N-dialkylaminoanilide derivative which is a novel substrate, or its salt.
CONSTITUTION: A novel substrate of formula (R1 and R2 are lower alkyl), e.g. L-leucyl-3-carboxy-4-N,N-dimethylaminoanilide, is converted by the enzymatic action of LAP to 2-dialkylamino-5-aminobenzoic acid, which is converted to a colored compound by the oxidative condensation with a proper coupler and subjected to colorimetry to determine the LAP activity. High solubility of the substrate in water enables easy preparation of the substrate solution. The reproducibility of the determination is good because of small variation of the color with temperature and time.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1602680A JPS56113299A (en) | 1980-02-14 | 1980-02-14 | Method and reagent for determining leucine aminopeptidase activity |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1602680A JPS56113299A (en) | 1980-02-14 | 1980-02-14 | Method and reagent for determining leucine aminopeptidase activity |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS56113299A true JPS56113299A (en) | 1981-09-07 |
JPS6261319B2 JPS6261319B2 (en) | 1987-12-21 |
Family
ID=11905047
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1602680A Granted JPS56113299A (en) | 1980-02-14 | 1980-02-14 | Method and reagent for determining leucine aminopeptidase activity |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56113299A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5963198A (en) * | 1982-10-01 | 1984-04-10 | Toyo Jozo Co Ltd | Quantitative assay using enzyme |
JPS5988099A (en) * | 1982-11-15 | 1984-05-21 | Toyo Jozo Co Ltd | Novel measurement of enzymatic activity |
-
1980
- 1980-02-14 JP JP1602680A patent/JPS56113299A/en active Granted
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5963198A (en) * | 1982-10-01 | 1984-04-10 | Toyo Jozo Co Ltd | Quantitative assay using enzyme |
JPH0358720B2 (en) * | 1982-10-01 | 1991-09-06 | Toyo Jozo Kk | |
JPS5988099A (en) * | 1982-11-15 | 1984-05-21 | Toyo Jozo Co Ltd | Novel measurement of enzymatic activity |
JPS6210160B2 (en) * | 1982-11-15 | 1987-03-04 | Toyo Jozo Kk |
Also Published As
Publication number | Publication date |
---|---|
JPS6261319B2 (en) | 1987-12-21 |
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