JPS5774096A - Measurement of enzyme activity - Google Patents

Measurement of enzyme activity

Info

Publication number
JPS5774096A
JPS5774096A JP13825081A JP13825081A JPS5774096A JP S5774096 A JPS5774096 A JP S5774096A JP 13825081 A JP13825081 A JP 13825081A JP 13825081 A JP13825081 A JP 13825081A JP S5774096 A JPS5774096 A JP S5774096A
Authority
JP
Japan
Prior art keywords
enzyme activity
act
leucylamide
leucyl
aminoacid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP13825081A
Other languages
Japanese (ja)
Inventor
Kunio Matsumoto
Toshiharu Noda
Tadayo Fujii
Susumu Watanabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyo Jozo KK
Original Assignee
Toyo Jozo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Jozo KK filed Critical Toyo Jozo KK
Priority to JP13825081A priority Critical patent/JPS5774096A/en
Publication of JPS5774096A publication Critical patent/JPS5774096A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: A sample for measuring enzyme activity is made act on L-leucylamide, then an immobilized oxidase is made to act on the reaction product to measure the amount of consumed oxygen or formed hydrogen peroxide, thus conducting the measurement of enzyme activity.
CONSTITUTION: An L-leucylamide of the formula (R is substituted methylamino group or residue of D-aminoacid) or its soluble salt is used as a substrate, on which a sample for measuring enzyme activity, which liberates methylamine or D-aminoacid by the reaction, is made to act. The L-leucylamide is, e.g., L-leucyl-benzylamide, L-leucylthylamine, L-leucyl-n-butylamide, L-leucyl-D-methionine or the like. An oxidase is made to act on the reaction product, i.e., substituted methylamine or D-aminoacid, to measure the amount of the consumed oxygen or formed hydrogen peroside, thus determining the activity.
COPYRIGHT: (C)1982,JPO&Japio
JP13825081A 1981-09-01 1981-09-01 Measurement of enzyme activity Pending JPS5774096A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP13825081A JPS5774096A (en) 1981-09-01 1981-09-01 Measurement of enzyme activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13825081A JPS5774096A (en) 1981-09-01 1981-09-01 Measurement of enzyme activity

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP4608580A Division JPS592278B2 (en) 1980-04-07 1980-04-07 Method for measuring leucine aminopeptidase activity

Publications (1)

Publication Number Publication Date
JPS5774096A true JPS5774096A (en) 1982-05-10

Family

ID=15217564

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13825081A Pending JPS5774096A (en) 1981-09-01 1981-09-01 Measurement of enzyme activity

Country Status (1)

Country Link
JP (1) JPS5774096A (en)

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