JPS5651998A - Novel method for detecting alkali phosphatase - Google Patents
Novel method for detecting alkali phosphataseInfo
- Publication number
- JPS5651998A JPS5651998A JP12845879A JP12845879A JPS5651998A JP S5651998 A JPS5651998 A JP S5651998A JP 12845879 A JP12845879 A JP 12845879A JP 12845879 A JP12845879 A JP 12845879A JP S5651998 A JPS5651998 A JP S5651998A
- Authority
- JP
- Japan
- Prior art keywords
- alkali phosphatase
- reacted
- nadh
- novel method
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To measure the activity of an alkali phosphatase contained in a living body sample, by using the change in amount of a coenzyme NADH2 formed.
CONSTITUTION: An alkali phosphatase in a living body sample is reacted with β- glycerophosphoric acid as a substrate, and the glycerol thus formed is reacted with glycerol dehydrogenase in the presence of NAD to measure the rate of formation of NADH2 and the activity of the alkali phosphatase. After fractionating the alkali phosphatase by electrophoresis with various supports, NADH2 is reacted with a diaphorase in the presence of nitroblue tetrazolium in the support, and the diformazan formed in the reaction is detected to detect the alkali phosphatase isoenzyme.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12845879A JPS5651998A (en) | 1979-10-06 | 1979-10-06 | Novel method for detecting alkali phosphatase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12845879A JPS5651998A (en) | 1979-10-06 | 1979-10-06 | Novel method for detecting alkali phosphatase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5651998A true JPS5651998A (en) | 1981-05-09 |
Family
ID=14985198
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12845879A Pending JPS5651998A (en) | 1979-10-06 | 1979-10-06 | Novel method for detecting alkali phosphatase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5651998A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0058539A1 (en) * | 1981-02-12 | 1982-08-25 | Colin Henry Self | Enzymatic detection method and use |
JPS59128196A (en) * | 1983-01-10 | 1984-07-24 | 株式会社日立製作所 | Chain hoist |
EP0427252A2 (en) * | 1989-11-08 | 1991-05-15 | Unitika Ltd. | Measurement of diaphorase activity and reagent therefor |
-
1979
- 1979-10-06 JP JP12845879A patent/JPS5651998A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0058539A1 (en) * | 1981-02-12 | 1982-08-25 | Colin Henry Self | Enzymatic detection method and use |
JPS59128196A (en) * | 1983-01-10 | 1984-07-24 | 株式会社日立製作所 | Chain hoist |
JPS6364398B2 (en) * | 1983-01-10 | 1988-12-12 | ||
EP0427252A2 (en) * | 1989-11-08 | 1991-05-15 | Unitika Ltd. | Measurement of diaphorase activity and reagent therefor |
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