JPS5636048A - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JPS5636048A JPS5636048A JP11188379A JP11188379A JPS5636048A JP S5636048 A JPS5636048 A JP S5636048A JP 11188379 A JP11188379 A JP 11188379A JP 11188379 A JP11188379 A JP 11188379A JP S5636048 A JPS5636048 A JP S5636048A
- Authority
- JP
- Japan
- Prior art keywords
- coenzyme
- dehydrogenase
- collecting body
- linking reagent
- enzyme electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To obtain an enzyme electrode by which the time required for measurement is shortened and which is excellent in sensitivity in an electrochemical measurement of the substrate concentration of an enzyme, by a method wherein oxidoreductase and coenzyme are fixed as film directly on a collecting body by a cross- linking reagent.
CONSTITUTION: A phosphate buffer solution of dehydrogenase (e.g. lactic dehydrogenase, LDH) and coenzyme thereof (e.g. nicotinamide adenine dinucleotide, NAD) is applied on to a collecting body (e.g. a graphite disc) and dried. Then, a cross- linking reagent is applied thereto, and the dehydrogenase and the coenzyme are stuck fast and fixed directly on to the collecting body as an insoluble film as a whole, to obtain an enzyme electrode. As the above-mentioned cross-linking reagent, glutaraldehyde, dimethyl adipoimidate or the like is employed.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11188379A JPS5636048A (en) | 1979-08-31 | 1979-08-31 | Enzyme electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11188379A JPS5636048A (en) | 1979-08-31 | 1979-08-31 | Enzyme electrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5636048A true JPS5636048A (en) | 1981-04-09 |
| JPS6235615B2 JPS6235615B2 (en) | 1987-08-03 |
Family
ID=14572533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11188379A Granted JPS5636048A (en) | 1979-08-31 | 1979-08-31 | Enzyme electrode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5636048A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61185442A (en) * | 1985-02-14 | 1986-08-19 | 平岡織染株式会社 | Heat-resistant sheet |
| JPS6275251A (en) * | 1985-09-25 | 1987-04-07 | ビオセンソリ ソチエタ ペル アツィオニ | Bioelectric chemical battery and electrode for said battery |
| WO2012026493A1 (en) * | 2010-08-26 | 2012-03-01 | アイシン精機株式会社 | Electrode having enzyme crystals immobilized thereon, process for production of electrode having enzyme crystals immobilized thereon, and biological fuel cell and biosensor each equipped with electrode having enzyme crystals immobilized thereon |
| JP2015136313A (en) * | 2014-01-21 | 2015-07-30 | 株式会社ダイセル | Cross-linked enzyme aggregate and microreactor comprising the same |
-
1979
- 1979-08-31 JP JP11188379A patent/JPS5636048A/en active Granted
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61185442A (en) * | 1985-02-14 | 1986-08-19 | 平岡織染株式会社 | Heat-resistant sheet |
| JPH0436063B2 (en) * | 1985-02-14 | 1992-06-15 | Hiraoka Shokusen | |
| JPS6275251A (en) * | 1985-09-25 | 1987-04-07 | ビオセンソリ ソチエタ ペル アツィオニ | Bioelectric chemical battery and electrode for said battery |
| WO2012026493A1 (en) * | 2010-08-26 | 2012-03-01 | アイシン精機株式会社 | Electrode having enzyme crystals immobilized thereon, process for production of electrode having enzyme crystals immobilized thereon, and biological fuel cell and biosensor each equipped with electrode having enzyme crystals immobilized thereon |
| US9303255B2 (en) | 2010-08-26 | 2016-04-05 | Aisin Seiki Kabushiki Kaisha | Electrode having enzyme crystals immobilized thereon, method for producing electrode having enzyme crystals immobilized thereon, and biological fuel cell and biosensor provided with electrode having enzyme crystals immobilized thereon |
| JP2015136313A (en) * | 2014-01-21 | 2015-07-30 | 株式会社ダイセル | Cross-linked enzyme aggregate and microreactor comprising the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6235615B2 (en) | 1987-08-03 |
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