JPS54139793A - Electrode - Google Patents
ElectrodeInfo
- Publication number
- JPS54139793A JPS54139793A JP4798578A JP4798578A JPS54139793A JP S54139793 A JPS54139793 A JP S54139793A JP 4798578 A JP4798578 A JP 4798578A JP 4798578 A JP4798578 A JP 4798578A JP S54139793 A JPS54139793 A JP S54139793A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- nad
- coenzyme
- sno
- reacted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To measure the substrate concentrations ob enzyme or activity of enzyme rapidly and readily by forming the enzyme electrode through directly and chemically bonding (particularly covalent bonding) coenzyme to current collectors and fixing the same and using this.
CONSTITUTION: Nicotineamide Adenine Dinucleotide (NAD, coenzyme) is reacted with, e.g., succinic acid anhydride to introduce carboxylic groups. On the other hand, SnO2 NESA glass (current collector) is subjected to hydrophilic treatment to form hydroxyl groups on the SnO2 surface, after which the above-mentioned denatured NAD is reacted to fix the NAD on the NESA glass through ester bond. After the NAD fixed electrode is formed by directly chemically bonding 4 the coenzyme 2 to the current collector 3 in the above-mentioned manner, it is dipped in a solution containing enzyme (e.g., lactic acid dehydrogenase) 1 to prepare the NAD- enzyme bond, which is then treated with crosslinking reagent (glutaraldehyde, etc.) to fix the enzyme. The substrate (lactic acid) concentration is measured by using this. If this is used in the state where enzyme is not bound, the measurement of enzyme activity is possible.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53047985A JPS5816694B2 (en) | 1978-04-21 | 1978-04-21 | electrode |
US06/194,271 US4321123A (en) | 1978-04-21 | 1980-10-06 | Coenzyme immobilized electrode |
US06/321,326 US4376689A (en) | 1978-04-21 | 1981-11-13 | Coenzyme immobilized electrode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP53047985A JPS5816694B2 (en) | 1978-04-21 | 1978-04-21 | electrode |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54139793A true JPS54139793A (en) | 1979-10-30 |
JPS5816694B2 JPS5816694B2 (en) | 1983-04-01 |
Family
ID=12790599
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53047985A Expired JPS5816694B2 (en) | 1978-04-21 | 1978-04-21 | electrode |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5816694B2 (en) |
-
1978
- 1978-04-21 JP JP53047985A patent/JPS5816694B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS5816694B2 (en) | 1983-04-01 |
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