JPS55148100A - Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide - Google Patents
Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxideInfo
- Publication number
- JPS55148100A JPS55148100A JP5651979A JP5651979A JPS55148100A JP S55148100 A JPS55148100 A JP S55148100A JP 5651979 A JP5651979 A JP 5651979A JP 5651979 A JP5651979 A JP 5651979A JP S55148100 A JPS55148100 A JP S55148100A
- Authority
- JP
- Japan
- Prior art keywords
- hydrogen peroxide
- aminotransferase
- determination
- acid
- reagent kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To determine aminotransferase in high sensitivity, by the use of a reagent kit for the determinaton of hydrogen peroxide composed of a p-halophenol and 4- aminoantipyrine.
CONSTITUTION: The activity of aminotransferase in a living specimen is determined by measuring hydrogen peroxide with a reagent kit composed of a p-halophenol of formula (X is halogen) and 4-aminoantipyrine. For example, a substrate containing alanine or aspartic acid and α-ketoglutaric acid is treated with aminotransferase, the produced oxalacetic acid is treated with an enzyme which eliminates carbon dioxide from oxalacetic acid, produced pyruvic acid is oxidized to acetyl phosphoric acid and hydrogen peroxide with pyruvic acid oxidase, and the hydrogen peroxide thus produced is determined by the above method.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5651979A JPS55148100A (en) | 1979-05-09 | 1979-05-09 | Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP5651979A JPS55148100A (en) | 1979-05-09 | 1979-05-09 | Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS55148100A true JPS55148100A (en) | 1980-11-18 |
Family
ID=13029358
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP5651979A Pending JPS55148100A (en) | 1979-05-09 | 1979-05-09 | Determination of aminotransferase using novel reagent kit for determination of hydrogen peroxide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55148100A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57208998A (en) * | 1981-06-17 | 1982-12-22 | Fuji Photo Film Co Ltd | Multi-layered analytical film for determination of transaminase |
-
1979
- 1979-05-09 JP JP5651979A patent/JPS55148100A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57208998A (en) * | 1981-06-17 | 1982-12-22 | Fuji Photo Film Co Ltd | Multi-layered analytical film for determination of transaminase |
JPH04640B2 (en) * | 1981-06-17 | 1992-01-08 | Fuji Photo Film Co Ltd |
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