JPS5631641A - New quantitative determination method for hydrogen peroxide - Google Patents
New quantitative determination method for hydrogen peroxideInfo
- Publication number
- JPS5631641A JPS5631641A JP10711479A JP10711479A JPS5631641A JP S5631641 A JPS5631641 A JP S5631641A JP 10711479 A JP10711479 A JP 10711479A JP 10711479 A JP10711479 A JP 10711479A JP S5631641 A JPS5631641 A JP S5631641A
- Authority
- JP
- Japan
- Prior art keywords
- replaced
- phenyl
- equation
- alkyl
- peroxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To enable to measure the H2O2 in living body liquid with long wave length at high sensitivity under the presence of peroxidase especially, by using the water-soluble reducing type color element of specific type.
CONSTITUTION: In equations I and II, (where; in equation I, R1 R2 are radicals expressed in equation II, and R3 is phenyl or naphthyl radical replaced with 1W3 sulfonic acid radicals (this phenyl or naphthyl radical can be replaced by OH, alkyl), R4, R5 are lower alkyl, H (replaced) phenyl, replaced radical is lower alkyl, and R6 is H, alkyl, carbonyl, sulfonic acid, halogen). For example, the reduction type of Patent Blue V in equation III is used as the color developing reagent dissolved in the phosphate buffer solution with peroxidase. The degree of light absorption around 640nm coloring by the addition of said reagent to the living body liquid such as serum is measured and the H2O2 in the sample is detected from the calibration curve obtained as to H2O2 aqueous solution separately in advance.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10711479A JPS5631641A (en) | 1979-08-24 | 1979-08-24 | New quantitative determination method for hydrogen peroxide |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10711479A JPS5631641A (en) | 1979-08-24 | 1979-08-24 | New quantitative determination method for hydrogen peroxide |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5631641A true JPS5631641A (en) | 1981-03-31 |
Family
ID=14450821
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10711479A Pending JPS5631641A (en) | 1979-08-24 | 1979-08-24 | New quantitative determination method for hydrogen peroxide |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5631641A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4804630A (en) * | 1987-03-13 | 1989-02-14 | Allied-Signal Inc. | Kit and method for detecting lithium ions |
US4810642A (en) * | 1985-06-26 | 1989-03-07 | Kyowa Medex Co., Ltd. | Method and test composition for determination of hydrogen peroxide |
US4988616A (en) * | 1986-06-10 | 1991-01-29 | Bayer Aktiengesellschaft | Method for detecting hydrogen peroxide employing triaryl- and trihetarylmethane derivatives as redox indicators |
US5194390A (en) * | 1988-07-05 | 1993-03-16 | Miles Inc. | Composition for the assay of albumin |
CN108822005A (en) * | 2018-06-13 | 2018-11-16 | 郑州大学 | A kind of reversible colorimetric probe and its preparation, application based on malachite green and bisulfite addition product |
WO2019235580A1 (en) * | 2018-06-07 | 2019-12-12 | キヤノン株式会社 | Dye for hydrogen peroxide detection, dye particle composite body, and structure for hydrogen peroxide detection |
-
1979
- 1979-08-24 JP JP10711479A patent/JPS5631641A/en active Pending
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4810642A (en) * | 1985-06-26 | 1989-03-07 | Kyowa Medex Co., Ltd. | Method and test composition for determination of hydrogen peroxide |
US4988616A (en) * | 1986-06-10 | 1991-01-29 | Bayer Aktiengesellschaft | Method for detecting hydrogen peroxide employing triaryl- and trihetarylmethane derivatives as redox indicators |
US4804630A (en) * | 1987-03-13 | 1989-02-14 | Allied-Signal Inc. | Kit and method for detecting lithium ions |
US5194390A (en) * | 1988-07-05 | 1993-03-16 | Miles Inc. | Composition for the assay of albumin |
WO2019235580A1 (en) * | 2018-06-07 | 2019-12-12 | キヤノン株式会社 | Dye for hydrogen peroxide detection, dye particle composite body, and structure for hydrogen peroxide detection |
CN108822005A (en) * | 2018-06-13 | 2018-11-16 | 郑州大学 | A kind of reversible colorimetric probe and its preparation, application based on malachite green and bisulfite addition product |
CN108822005B (en) * | 2018-06-13 | 2021-04-09 | 郑州大学 | Reversible colorimetric probe based on malachite green and bisulfite addition product, and preparation and application thereof |
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