FI933868A - Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen - Google Patents

Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen Download PDF

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Publication number
FI933868A
FI933868A FI933868A FI933868A FI933868A FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A
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FI
Finland
Prior art keywords
heterologous
gene
expression
produced
activation
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FI933868A
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English (en)
Swedish (sv)
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FI933868A0 (fi
FI95578B (fi
FI95578C (fi
Inventor
Rainer Rudolph
Stephan Fischer
Ralf Mattes
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Boehringer Mannheim Gmbh
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Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of FI933868A0 publication Critical patent/FI933868A0/fi
Publication of FI933868A publication Critical patent/FI933868A/fi
Publication of FI95578B publication Critical patent/FI95578B/fi
Application granted granted Critical
Publication of FI95578C publication Critical patent/FI95578C/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
FI933868A 1985-10-23 1993-09-03 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen FI95578C (fi)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE3537708 1985-10-23
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten
EP8600610 1986-10-23
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
FI872753A FI94050C (fi) 1985-10-23 1987-06-22 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen
FI872753 1987-06-22

Publications (4)

Publication Number Publication Date
FI933868A0 FI933868A0 (fi) 1993-09-03
FI933868A true FI933868A (fi) 1993-09-03
FI95578B FI95578B (fi) 1995-11-15
FI95578C FI95578C (fi) 1996-02-26

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (fi) 1985-10-23 1987-06-22 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen
FI933868A FI95578C (fi) 1985-10-23 1993-09-03 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (fi) 1985-10-23 1987-06-22 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen

Country Status (26)

Country Link
EP (3) EP0219874B1 (fi)
JP (2) JPH0728745B2 (fi)
KR (1) KR900009139B1 (fi)
AT (2) ATE131489T1 (fi)
AU (2) AU590029B2 (fi)
CA (1) CA1329157C (fi)
CZ (1) CZ280727B6 (fi)
DD (1) DD260517A5 (fi)
DE (3) DE3537708A1 (fi)
DK (2) DK175091B1 (fi)
ES (2) ES2061434T3 (fi)
FI (2) FI94050C (fi)
GR (2) GR920300062T1 (fi)
HK (2) HK153496A (fi)
HR (1) HRP921075B1 (fi)
HU (2) HUT43643A (fi)
IE (1) IE62634B1 (fi)
IL (1) IL80325A (fi)
PT (1) PT83609B (fi)
SI (1) SI8611796B (fi)
SK (1) SK278317B6 (fi)
SU (1) SU1607689A3 (fi)
UA (1) UA6023A1 (fi)
WO (1) WO1987002673A2 (fi)
YU (1) YU47185B (fi)
ZA (1) ZA868012B (fi)

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WO1988008428A1 (en) 1987-04-28 1988-11-03 Amgen Inc. Method for purifying granulocyte/macrophage colony stimulating factor
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
WO1992003477A1 (en) * 1990-08-20 1992-03-05 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
EP0586667A1 (en) * 1992-03-24 1994-03-16 Synergen, Inc. Refolding and purification of insulin-like growth factor i
EP0600372B1 (de) 1992-12-02 1997-02-05 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
JP3288720B2 (ja) * 1996-06-11 2002-06-04 ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング 変性タンパク質の活性化方法
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
DE60129432T2 (de) 2000-05-16 2008-04-17 Bolder Biotechnology, Inc., Louisville Verfahren zur rückfaltung von proteinen mit freien cysteinresten
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE102006009437A1 (de) 2006-03-01 2007-09-13 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
SG162834A1 (en) 2006-07-14 2010-07-29 Genentech Inc Refolding of recombinant proteins
US8617531B2 (en) 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine
NZ601759A (en) 2010-03-17 2013-07-26 Biogenerix Gmbh Method for obtaining biologically active recombinant human g-csf
AU2011317021B2 (en) * 2010-10-20 2015-07-09 Medimmune, Llc Methods for processing inclusion bodies
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
NZ746109A (en) 2016-12-30 2022-12-23 Biogend Therapeutics Co Ltd Recombinant polypeptides and nucleic acid molecules, compositions, and methods of making and uses thereof
US20240101598A1 (en) 2020-12-18 2024-03-28 Richter Gedeon Nyrt. Methods for the purification of refolded fc-peptide fusion protein

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Also Published As

Publication number Publication date
CA1329157C (en) 1994-05-03
AU590029B2 (en) 1989-10-26
KR870700601A (ko) 1987-12-30
DK320387A (da) 1987-06-23
EP0393725A1 (de) 1990-10-24
FI94050B (fi) 1995-03-31
FI933868A0 (fi) 1993-09-03
JPH04218387A (ja) 1992-08-07
DK320387D0 (da) 1987-06-23
HRP921075B1 (en) 1999-02-28
PT83609A (de) 1986-11-01
ATE131489T1 (de) 1995-12-15
DE3689404D1 (de) 1994-01-27
EP0219874A3 (en) 1988-02-10
JPH0824594B2 (ja) 1996-03-13
EP0219874B1 (de) 1993-12-15
WO1987002673A2 (en) 1987-05-07
EP0219874A2 (de) 1987-04-29
EP0253823A1 (de) 1988-01-27
FI872753A (fi) 1987-06-22
CZ752686A3 (en) 1996-01-17
DE3537708C2 (fi) 1993-07-08
HU204855B (en) 1992-02-28
FI872753A0 (fi) 1987-06-22
IE862683L (en) 1987-04-23
YU179686A (en) 1988-06-30
AU4132189A (en) 1990-01-04
IL80325A0 (en) 1987-01-30
SU1607689A3 (ru) 1990-11-15
ES2020498T3 (es) 1996-04-01
EP0393725B1 (de) 1995-12-13
PT83609B (pt) 1988-10-14
JPS62502895A (ja) 1987-11-19
DE3537708A1 (de) 1987-04-23
ES2061434T3 (es) 1994-12-16
GR3018410T3 (en) 1996-03-31
HK153596A (en) 1996-08-16
CZ280727B6 (cs) 1996-04-17
DD260517A5 (de) 1988-09-28
DE3650449D1 (de) 1996-01-25
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
AU6599386A (en) 1987-05-19
JPH0728745B2 (ja) 1995-04-05
DK175091B1 (da) 2004-05-24
HK153496A (en) 1996-08-16
ES2020498A4 (es) 1991-08-16
FI94050C (fi) 1995-07-10
SK278317B6 (en) 1996-10-02
UA6023A1 (uk) 1994-12-29
ATE98648T1 (de) 1994-01-15
AU607083B2 (en) 1991-02-21
HUT43643A (en) 1987-11-30
SI8611796B (sl) 1998-06-30
IE62634B1 (en) 1995-02-22
IL80325A (en) 1992-06-21
DK200001897A (da) 2000-12-18
SI8611796A (sl) 1996-10-31
KR900009139B1 (ko) 1990-12-22
HRP921075A2 (en) 1995-06-30
WO1987002673A3 (fr) 1987-10-22
DK175109B1 (da) 2004-06-07
YU47185B (sh) 1995-01-31
SK752686A3 (en) 1996-10-01
ZA868012B (en) 1987-06-24
GR920300062T1 (en) 1992-08-31

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