HK153596A - Process for activating genetically-engineered heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes - Google Patents

Process for activating genetically-engineered heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes

Info

Publication number
HK153596A
HK153596A HK153596A HK153596A HK153596A HK 153596 A HK153596 A HK 153596A HK 153596 A HK153596 A HK 153596A HK 153596 A HK153596 A HK 153596A HK 153596 A HK153596 A HK 153596A
Authority
HK
Hong Kong
Prior art keywords
pref
expression
procaryotes
esp
proteins containing
Prior art date
Application number
HK153596A
Inventor
Stephan Dr Fischer
Rudolph Dr Rainer
Ralf Dr Mattes
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of HK153596A publication Critical patent/HK153596A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Method for activating non-glycosylated tissue plasminogen activator (t-PA) after its expression in prokaryotic cells comprises cell lysis; solubilisation under denaturing and reducing conditions, and reactivation under oxidising conditions in presence of reduced and oxidised glutathione (G5H, G55G). The new feature is that in the last stage is at pH 9-12 (pref. 9.5-11) with G5H and G55G concns. 0.1-20, pref. 0.2-10, mM and 0.01-3, pref. 0.5-1, mM, respectively, and with a non-denaturing concn. of the denaturing agent. Esp. the method is applied to t-PA expressed in E.coli and P. putida. The denaturing agent is pref. arginine, guanidine hydrochloride (both at 0.1-1, esp. 0.25-0.75, mM) or urea, at 0.5-4 (esp. 1-3.5) M in the last stage.
HK153596A 1985-10-23 1996-08-08 Process for activating genetically-engineered heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes HK153596A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (en) 1985-10-23 1985-10-23 METHOD FOR ACTIVATING T-PA AFTER EXPRESSION IN PROKARYONTS

Publications (1)

Publication Number Publication Date
HK153596A true HK153596A (en) 1996-08-16

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
HK153496A HK153496A (en) 1985-10-23 1996-08-08 Process for activating recombinant heterologous eucaryotic disulfide-bridged proteins after expression by procaryotes
HK153596A HK153596A (en) 1985-10-23 1996-08-08 Process for activating genetically-engineered heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
HK153496A HK153496A (en) 1985-10-23 1996-08-08 Process for activating recombinant heterologous eucaryotic disulfide-bridged proteins after expression by procaryotes

Country Status (26)

Country Link
EP (3) EP0219874B1 (en)
JP (2) JPH0728745B2 (en)
KR (1) KR900009139B1 (en)
AT (2) ATE131489T1 (en)
AU (2) AU590029B2 (en)
CA (1) CA1329157C (en)
CZ (1) CZ280727B6 (en)
DD (1) DD260517A5 (en)
DE (3) DE3537708A1 (en)
DK (2) DK175091B1 (en)
ES (2) ES2020498T3 (en)
FI (2) FI94050C (en)
GR (2) GR920300062T1 (en)
HK (2) HK153496A (en)
HR (1) HRP921075B1 (en)
HU (2) HUT43643A (en)
IE (1) IE62634B1 (en)
IL (1) IL80325A (en)
PT (1) PT83609B (en)
SI (1) SI8611796B (en)
SK (1) SK278317B6 (en)
SU (1) SU1607689A3 (en)
UA (1) UA6023A1 (en)
WO (1) WO1987002673A2 (en)
YU (1) YU47185B (en)
ZA (1) ZA868012B (en)

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DE3832898A1 (en) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
DE3835350A1 (en) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh ACTIVATION OF GENETICALLY MANUFACTURED ANTIBODY EXPRESSED IN PROKARYONS
DE3903581A1 (en) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE
DE3942143A1 (en) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-PA PRO STABILIZATION
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DE4139000A1 (en) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh METHOD OF GENERATING BIOLOGICALLY ACTIVE SS-NGF
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DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
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US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
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EP1048732A1 (en) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins
EP1077263A1 (en) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins by co-secretion of chaperones
DE60129432T2 (en) 2000-05-16 2008-04-17 Bolder Biotechnology, Inc., Louisville PROCESS FOR THE RECYCLING OF PROTEINS WITH FREE CYSTEIN REST
DE10105912A1 (en) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
DE10105911A1 (en) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression of the recombinant proteinase K from Tritirachium album in yeast
DE102005033250A1 (en) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Process for purifying G-CSF
DE202006020194U1 (en) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF liquid formulation
WO2008008975A2 (en) 2006-07-14 2008-01-17 Genentech, Inc. Refolding of recombinant proteins
EP2102355B1 (en) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same
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Also Published As

Publication number Publication date
HUT43643A (en) 1987-11-30
DD260517A5 (en) 1988-09-28
EP0219874A2 (en) 1987-04-29
ES2061434T3 (en) 1994-12-16
UA6023A1 (en) 1994-12-29
EP0253823A1 (en) 1988-01-27
WO1987002673A2 (en) 1987-05-07
ZA868012B (en) 1987-06-24
ATE98648T1 (en) 1994-01-15
CA1329157C (en) 1994-05-03
FI933868A0 (en) 1993-09-03
IE62634B1 (en) 1995-02-22
AU4132189A (en) 1990-01-04
IL80325A (en) 1992-06-21
IL80325A0 (en) 1987-01-30
FI94050B (en) 1995-03-31
FI95578B (en) 1995-11-15
FI95578C (en) 1996-02-26
DK320387D0 (en) 1987-06-23
ES2020498A4 (en) 1991-08-16
YU179686A (en) 1988-06-30
FI872753A0 (en) 1987-06-22
FI872753A (en) 1987-06-22
CZ752686A3 (en) 1996-01-17
DE3537708C2 (en) 1993-07-08
DK200001897A (en) 2000-12-18
PT83609A (en) 1986-11-01
ES2020498T3 (en) 1996-04-01
DK175091B1 (en) 2004-05-24
JPS62502895A (en) 1987-11-19
AU590029B2 (en) 1989-10-26
HRP921075A2 (en) 1995-06-30
HK153496A (en) 1996-08-16
KR900009139B1 (en) 1990-12-22
DK175109B1 (en) 2004-06-07
YU47185B (en) 1995-01-31
SU1607689A3 (en) 1990-11-15
DE3650449D1 (en) 1996-01-25
EP0219874A3 (en) 1988-02-10
GR920300062T1 (en) 1992-08-31
AU6599386A (en) 1987-05-19
DE3537708A1 (en) 1987-04-23
JPH04218387A (en) 1992-08-07
PT83609B (en) 1988-10-14
IE862683L (en) 1987-04-23
SK278317B6 (en) 1996-10-02
GR3018410T3 (en) 1996-03-31
WO1987002673A3 (en) 1987-10-22
EP0393725A1 (en) 1990-10-24
JPH0824594B2 (en) 1996-03-13
EP0393725B1 (en) 1995-12-13
ATE131489T1 (en) 1995-12-15
HRP921075B1 (en) 1999-02-28
SK752686A3 (en) 1996-10-01
FI933868A (en) 1993-09-03
EP0219874B1 (en) 1993-12-15
AU607083B2 (en) 1991-02-21
DE3689404D1 (en) 1994-01-27
KR870700601A (en) 1987-12-30
SI8611796B (en) 1998-06-30
FI94050C (en) 1995-07-10
JPH0728745B2 (en) 1995-04-05
DK320387A (en) 1987-06-23
HU204855B (en) 1992-02-28
CZ280727B6 (en) 1996-04-17
SI8611796A (en) 1996-10-31

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