FI933868A - Method for Activation of Gene Technologist Produced, Heterologous, Eukaryotic Proteins Showing Disulfide Crosslinks after Expression in Prokaryotic Cells - Google Patents

Method for Activation of Gene Technologist Produced, Heterologous, Eukaryotic Proteins Showing Disulfide Crosslinks after Expression in Prokaryotic Cells Download PDF

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Publication number
FI933868A
FI933868A FI933868A FI933868A FI933868A FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A
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FI
Finland
Prior art keywords
heterologous
gene
expression
produced
activation
Prior art date
Application number
FI933868A
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Finnish (fi)
Swedish (sv)
Other versions
FI95578B (en
FI95578C (en
FI933868A0 (en
Inventor
Rainer Rudolph
Stephan Fischer
Ralf Mattes
Original Assignee
Boehringer Mannheim Gmbh
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Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of FI933868A publication Critical patent/FI933868A/en
Publication of FI933868A0 publication Critical patent/FI933868A0/en
Publication of FI95578B publication Critical patent/FI95578B/en
Application granted granted Critical
Publication of FI95578C publication Critical patent/FI95578C/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The invention relates to a process for the activating of gene-technologically produced, heterologous, disulphide bridge-containing eukaryotic proteins after expression in prokaryotes by crushing the cells and solubilizing proteins under denaturing and reducing conditions; said products are activated under oxidizing conditions in the presence of compounds GSH and GSSG, wherein during the activation stage of the process the pH is 9 to 12, GSH is at a concentration of 0.1 to 20 mmol/l, GSSG is at a concentration of 0.01 to 3 mmol/l and a denaturing agent present is at a non-denaturing concentration. The process is particularly valuable for t-Pa protein and beta interferon.
FI933868A 1985-10-23 1993-09-03 Method for activating genetically engineered heterologous disulfide bridge eukaryotic proteins after expression in prokaryotes FI95578C (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (en) 1985-10-23 1985-10-23 METHOD FOR ACTIVATING T-PA AFTER EXPRESSION IN PROKARYONTS
DE3537708 1985-10-23
EP8600610 1986-10-23
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
FI872753 1987-06-22
FI872753A FI94050C (en) 1985-10-23 1987-06-22 Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes

Publications (4)

Publication Number Publication Date
FI933868A true FI933868A (en) 1993-09-03
FI933868A0 FI933868A0 (en) 1993-09-03
FI95578B FI95578B (en) 1995-11-15
FI95578C FI95578C (en) 1996-02-26

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (en) 1985-10-23 1987-06-22 Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes
FI933868A FI95578C (en) 1985-10-23 1993-09-03 Method for activating genetically engineered heterologous disulfide bridge eukaryotic proteins after expression in prokaryotes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (en) 1985-10-23 1987-06-22 Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes

Country Status (26)

Country Link
EP (3) EP0393725B1 (en)
JP (2) JPH0728745B2 (en)
KR (1) KR900009139B1 (en)
AT (2) ATE98648T1 (en)
AU (2) AU590029B2 (en)
CA (1) CA1329157C (en)
CZ (1) CZ280727B6 (en)
DD (1) DD260517A5 (en)
DE (3) DE3537708A1 (en)
DK (2) DK175091B1 (en)
ES (2) ES2061434T3 (en)
FI (2) FI94050C (en)
GR (2) GR920300062T1 (en)
HK (2) HK153596A (en)
HR (1) HRP921075B1 (en)
HU (2) HUT43643A (en)
IE (1) IE62634B1 (en)
IL (1) IL80325A (en)
PT (1) PT83609B (en)
SI (1) SI8611796B (en)
SK (1) SK278317B6 (en)
SU (1) SU1607689A3 (en)
UA (1) UA6023A1 (en)
WO (1) WO1987002673A2 (en)
YU (1) YU47185B (en)
ZA (1) ZA868012B (en)

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US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (en) * 1987-04-28 1988-11-03 Amgen Inc. Method for purifying granulocyte/macrophage colony stimulating factor
DE3722082A1 (en) * 1987-07-03 1989-01-12 Behringwerke Ag METHOD FOR DETERMINING THE ACTIVITY OF SERINE PROTEASES OR SERINE PROTEASE INHIBITORS
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (en) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
DE3835350A1 (en) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh ACTIVATION OF GENETICALLY MANUFACTURED ANTIBODY EXPRESSED IN PROKARYONS
DE3903581A1 (en) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE
DE3942143A1 (en) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-PA PRO STABILIZATION
JPH06500084A (en) * 1990-08-20 1994-01-06 ノボ　ノルディスク　アクティーゼルスカブ Biologically active compounds, their production methods and their uses
EP0547102B1 (en) * 1990-09-05 1998-07-08 Southern Cross Biotech Pty.Ltd. Solubilization of proteins in active forms
DE4037196A1 (en) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh METHOD FOR REACTIVATING DENATURED PROTEIN
DE4113750A1 (en) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
DE4139000A1 (en) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh METHOD OF GENERATING BIOLOGICALLY ACTIVE SS-NGF
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
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DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
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US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (en) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragments
EP1048732A1 (en) 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins
EP1077263A1 (en) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins by co-secretion of chaperones
JP4873818B2 (en) 2000-05-16 2012-02-08 ボルダー バイオテクノロジー， インコーポレイテッド Methods for refolding proteins containing free cysteine residues
DE10105911A1 (en) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression of the recombinant proteinase K from Tritirachium album in yeast
DE10105912A1 (en) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
DE102005033250A1 (en) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Process for purifying G-CSF
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Also Published As

Publication number Publication date
EP0219874B1 (en) 1993-12-15
DK200001897A (en) 2000-12-18
DE3537708A1 (en) 1987-04-23
KR900009139B1 (en) 1990-12-22
PT83609B (en) 1988-10-14
HRP921075A2 (en) 1995-06-30
HK153496A (en) 1996-08-16
FI872753A0 (en) 1987-06-22
CZ280727B6 (en) 1996-04-17
DK320387D0 (en) 1987-06-23
EP0219874A3 (en) 1988-02-10
ES2061434T3 (en) 1994-12-16
PT83609A (en) 1986-11-01
GR3018410T3 (en) 1996-03-31
UA6023A1 (en) 1994-12-29
JPH04218387A (en) 1992-08-07
DE3650449D1 (en) 1996-01-25
HK153596A (en) 1996-08-16
IL80325A (en) 1992-06-21
EP0253823A1 (en) 1988-01-27
HUT43643A (en) 1987-11-30
HU204855B (en) 1992-02-28
FI95578B (en) 1995-11-15
FI95578C (en) 1996-02-26
YU47185B (en) 1995-01-31
AU590029B2 (en) 1989-10-26
SK752686A3 (en) 1996-10-01
GR920300062T1 (en) 1992-08-31
CZ752686A3 (en) 1996-01-17
DE3537708C2 (en) 1993-07-08
IL80325A0 (en) 1987-01-30
DK175109B1 (en) 2004-06-07
AU6599386A (en) 1987-05-19
EP0219874A2 (en) 1987-04-29
ES2020498T3 (en) 1996-04-01
EP0393725A1 (en) 1990-10-24
JPH0824594B2 (en) 1996-03-13
WO1987002673A2 (en) 1987-05-07
YU179686A (en) 1988-06-30
SI8611796A (en) 1996-10-31
DK320387A (en) 1987-06-23
FI94050C (en) 1995-07-10
WO1987002673A3 (en) 1987-10-22
DE3689404D1 (en) 1994-01-27
ES2020498A4 (en) 1991-08-16
JPS62502895A (en) 1987-11-19
SU1607689A3 (en) 1990-11-15
DD260517A5 (en) 1988-09-28
HRP921075B1 (en) 1999-02-28
ATE131489T1 (en) 1995-12-15
ZA868012B (en) 1987-06-24
CA1329157C (en) 1994-05-03
JPH0728745B2 (en) 1995-04-05
AU607083B2 (en) 1991-02-21
IE862683L (en) 1987-04-23
AU4132189A (en) 1990-01-04
EP0393725B1 (en) 1995-12-13
SK278317B6 (en) 1996-10-02
FI94050B (en) 1995-03-31
IE62634B1 (en) 1995-02-22
KR870700601A (en) 1987-12-30
ATE98648T1 (en) 1994-01-15
FI872753A (en) 1987-06-22
DK175091B1 (en) 2004-05-24
SI8611796B (en) 1998-06-30
FI933868A0 (en) 1993-09-03

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