DK320387D0 - PROCEDURE FOR ACTIVATING GENTECHNOLOGICALLY MANUFACTURED, HETEROLOGIST, DISULFID BRIDGES CONTAINING EUCARYOTIC PROTEINS AFTER EXPRESSION IN PROCARYOTIC CELLS - Google Patents

PROCEDURE FOR ACTIVATING GENTECHNOLOGICALLY MANUFACTURED, HETEROLOGIST, DISULFID BRIDGES CONTAINING EUCARYOTIC PROTEINS AFTER EXPRESSION IN PROCARYOTIC CELLS

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Publication number
DK320387D0
DK320387D0 DK320387A DK320387A DK320387D0 DK 320387 D0 DK320387 D0 DK 320387D0 DK 320387 A DK320387 A DK 320387A DK 320387 A DK320387 A DK 320387A DK 320387 D0 DK320387 D0 DK 320387D0
Authority
DK
Denmark
Prior art keywords
expression
pref
activating
gentechnologically
heterologist
Prior art date
Application number
DK320387A
Other languages
Danish (da)
Other versions
DK320387A (en
DK175091B1 (en
Inventor
Stephan Fischer
Ralf Mattes
Rainer Rudolph
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of DK320387D0 publication Critical patent/DK320387D0/en
Publication of DK320387A publication Critical patent/DK320387A/en
Priority to DK200001897A priority Critical patent/DK175109B1/en
Application granted granted Critical
Publication of DK175091B1 publication Critical patent/DK175091B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Lubricants (AREA)

Abstract

Method for activating non-glycosylated tissue plasminogen activator (t-PA) after its expression in prokaryotic cells comprises cell lysis; solubilisation under denaturing and reducing conditions, and reactivation under oxidising conditions in presence of reduced and oxidised glutathione (G5H, G55G). The new feature is that in the last stage is at pH 9-12 (pref. 9.5-11) with G5H and G55G concns. 0.1-20, pref. 0.2-10, mM and 0.01-3, pref. 0.5-1, mM, respectively, and with a non-denaturing concn. of the denaturing agent. Esp. the method is applied to t-PA expressed in E.coli and P. putida. The denaturing agent is pref. arginine, guanidine hydrochloride (both at 0.1-1, esp. 0.25-0.75, mM) or urea, at 0.5-4 (esp. 1-3.5) M in the last stage.
DK198703203A 1985-10-23 1987-06-23 Method for activating gene-technologically prepared, heterologous, disulfide-bridged eukaryotic proteins after expression in prokaryotic cells DK175091B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK200001897A DK175109B1 (en) 1985-10-23 2000-12-18 Activating recombinant non-glycosylated tissue plasminogen activator

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (en) 1985-10-23 1985-10-23 METHOD FOR ACTIVATING T-PA AFTER EXPRESSION IN PROKARYONTS
DE3537708 1985-10-23
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
EP8600610 1986-10-23

Publications (3)

Publication Number Publication Date
DK320387D0 true DK320387D0 (en) 1987-06-23
DK320387A DK320387A (en) 1987-06-23
DK175091B1 DK175091B1 (en) 2004-05-24

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
DK198703203A DK175091B1 (en) 1985-10-23 1987-06-23 Method for activating gene-technologically prepared, heterologous, disulfide-bridged eukaryotic proteins after expression in prokaryotic cells
DK200001897A DK175109B1 (en) 1985-10-23 2000-12-18 Activating recombinant non-glycosylated tissue plasminogen activator

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK200001897A DK175109B1 (en) 1985-10-23 2000-12-18 Activating recombinant non-glycosylated tissue plasminogen activator

Country Status (26)

Country Link
EP (3) EP0393725B1 (en)
JP (2) JPH0728745B2 (en)
KR (1) KR900009139B1 (en)
AT (2) ATE98648T1 (en)
AU (2) AU590029B2 (en)
CA (1) CA1329157C (en)
CZ (1) CZ280727B6 (en)
DD (1) DD260517A5 (en)
DE (3) DE3537708A1 (en)
DK (2) DK175091B1 (en)
ES (2) ES2020498T3 (en)
FI (2) FI94050C (en)
GR (2) GR920300062T1 (en)
HK (2) HK153496A (en)
HR (1) HRP921075B1 (en)
HU (2) HUT43643A (en)
IE (1) IE62634B1 (en)
IL (1) IL80325A (en)
PT (1) PT83609B (en)
SI (1) SI8611796B (en)
SK (1) SK278317B6 (en)
SU (1) SU1607689A3 (en)
UA (1) UA6023A1 (en)
WO (1) WO1987002673A2 (en)
YU (1) YU47185B (en)
ZA (1) ZA868012B (en)

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JP2581668B2 (en) * 1985-11-27 1997-02-12 三井東圧化学株式会社 Novel DNA sequence encoding human tissue plasminogen activator derived from normal human cells and vectors and cells containing the same
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (en) * 1987-04-28 1988-11-03 Amgen Inc. Method for purifying granulocyte/macrophage colony stimulating factor
DE3722082A1 (en) * 1987-07-03 1989-01-12 Behringwerke Ag METHOD FOR DETERMINING THE ACTIVITY OF SERINE PROTEASES OR SERINE PROTEASE INHIBITORS
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (en) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
DE3835350A1 (en) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh ACTIVATION OF GENETICALLY MANUFACTURED ANTIBODY EXPRESSED IN PROKARYONS
DE3903581A1 (en) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE
DE3942143A1 (en) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-PA PRO STABILIZATION
ATE154073T1 (en) * 1990-08-20 1997-06-15 Novo Nordisk As PROCESS FOR PRODUCING BIOLOGICALLY ACTIVE IGF-1 BY USING AMINO-TERMINAL EXTENDED IGF-1
ES2119779T3 (en) * 1990-09-05 1998-10-16 Southern Cross Biotech Pty Ltd SOLUBILIZATION OF PROTEINS IN ACTIVE FORMS.
DE4037196A1 (en) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh METHOD FOR REACTIVATING DENATURED PROTEIN
DE4113750A1 (en) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
DE4139000A1 (en) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh METHOD OF GENERATING BIOLOGICALLY ACTIVE SS-NGF
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
EP0586667A1 (en) * 1992-03-24 1994-03-16 Synergen, Inc. Refolding and purification of insulin-like growth factor i
ES2097426T3 (en) 1992-12-02 1997-04-01 Hoechst Ag PROCEDURE FOR OBTAINING PROINSULIN WITH CORRECTLY UNITED CYSTINE BRIDGES.
DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
FR2729972B1 (en) * 1995-01-31 1997-04-18 Sanofi Sa PROCESS FOR THE EXTRACTION OF PERIPLASMIC PROTEINS FROM PROKARYOTIC MICROORGANISMS IN THE PRESENCE OF ARGININ
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
BR9709569B1 (en) * 1996-06-11 2010-05-18 process for activating denatured protein.
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (en) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragments
EP1048732A1 (en) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins
EP1077263A1 (en) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins by co-secretion of chaperones
AU7485301A (en) 2000-05-16 2001-11-26 Bolder Biotechnology Inc Methods for refolding proteins containing free cysteine residues
DE10105911A1 (en) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression of the recombinant proteinase K from Tritirachium album in yeast
DE10105912A1 (en) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
DE102005033250A1 (en) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Process for purifying G-CSF
DE102006009437A1 (en) 2006-03-01 2007-09-13 Bioceuticals Arzneimittel Ag G-CSF liquid formulation
ZA200900229B (en) 2006-07-14 2010-04-28 Genentech Inc Refolding of recombinant proteins
US8617531B2 (en) 2006-12-14 2013-12-31 Bolder Biotechnology, Inc. Methods of making proteins and peptides containing a single free cysteine
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HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (en) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Methods for the production of polypeptides
CN103852527B (en) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 High-flux protein sample pre-treatment device
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Also Published As

Publication number Publication date
KR870700601A (en) 1987-12-30
ATE131489T1 (en) 1995-12-15
ES2020498A4 (en) 1991-08-16
JPH0824594B2 (en) 1996-03-13
HK153496A (en) 1996-08-16
HUT43643A (en) 1987-11-30
PT83609B (en) 1988-10-14
CA1329157C (en) 1994-05-03
DE3650449D1 (en) 1996-01-25
FI94050B (en) 1995-03-31
AU590029B2 (en) 1989-10-26
YU47185B (en) 1995-01-31
WO1987002673A2 (en) 1987-05-07
SK278317B6 (en) 1996-10-02
IE62634B1 (en) 1995-02-22
DD260517A5 (en) 1988-09-28
GR920300062T1 (en) 1992-08-31
FI94050C (en) 1995-07-10
DE3689404D1 (en) 1994-01-27
CZ280727B6 (en) 1996-04-17
ZA868012B (en) 1987-06-24
JPH04218387A (en) 1992-08-07
SI8611796A (en) 1996-10-31
HU204855B (en) 1992-02-28
AU607083B2 (en) 1991-02-21
WO1987002673A3 (en) 1987-10-22
YU179686A (en) 1988-06-30
ES2020498T3 (en) 1996-04-01
IL80325A (en) 1992-06-21
DK175109B1 (en) 2004-06-07
EP0219874A3 (en) 1988-02-10
IL80325A0 (en) 1987-01-30
FI933868A (en) 1993-09-03
SK752686A3 (en) 1996-10-01
DE3537708A1 (en) 1987-04-23
FI95578B (en) 1995-11-15
FI95578C (en) 1996-02-26
GR3018410T3 (en) 1996-03-31
DK320387A (en) 1987-06-23
EP0253823A1 (en) 1988-01-27
ES2061434T3 (en) 1994-12-16
FI872753A (en) 1987-06-22
JPH0728745B2 (en) 1995-04-05
EP0219874B1 (en) 1993-12-15
EP0219874A2 (en) 1987-04-29
SU1607689A3 (en) 1990-11-15
CZ752686A3 (en) 1996-01-17
UA6023A1 (en) 1994-12-29
EP0393725A1 (en) 1990-10-24
HK153596A (en) 1996-08-16
AU6599386A (en) 1987-05-19
FI872753A0 (en) 1987-06-22
IE862683L (en) 1987-04-23
PT83609A (en) 1986-11-01
DK200001897A (en) 2000-12-18
DK175091B1 (en) 2004-05-24
ATE98648T1 (en) 1994-01-15
EP0393725B1 (en) 1995-12-13
AU4132189A (en) 1990-01-04
KR900009139B1 (en) 1990-12-22
HRP921075A2 (en) 1995-06-30
SI8611796B (en) 1998-06-30
HRP921075B1 (en) 1999-02-28
JPS62502895A (en) 1987-11-19
FI933868A0 (en) 1993-09-03
DE3537708C2 (en) 1993-07-08

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