FI872753A0 - Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes - Google Patents

Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes

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Publication number
FI872753A0
FI872753A0 FI872753A FI872753A FI872753A0 FI 872753 A0 FI872753 A0 FI 872753A0 FI 872753 A FI872753 A FI 872753A FI 872753 A FI872753 A FI 872753A FI 872753 A0 FI872753 A0 FI 872753A0
Authority
FI
Finland
Prior art keywords
expression
pref
prokaryotes
esp
eukaryotic proteins
Prior art date
Application number
FI872753A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI94050B (en
FI872753A (en
FI94050C (en
Inventor
Rainer Rudolph
Stephan Fischer
Ralf Mattes
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of FI872753A publication Critical patent/FI872753A/en
Publication of FI872753A0 publication Critical patent/FI872753A0/en
Priority to FI933868A priority Critical patent/FI95578C/en
Publication of FI94050B publication Critical patent/FI94050B/en
Application granted granted Critical
Publication of FI94050C publication Critical patent/FI94050C/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Abstract

Method for activating non-glycosylated tissue plasminogen activator (t-PA) after its expression in prokaryotic cells comprises cell lysis; solubilisation under denaturing and reducing conditions, and reactivation under oxidising conditions in presence of reduced and oxidised glutathione (G5H, G55G). The new feature is that in the last stage is at pH 9-12 (pref. 9.5-11) with G5H and G55G concns. 0.1-20, pref. 0.2-10, mM and 0.01-3, pref. 0.5-1, mM, respectively, and with a non-denaturing concn. of the denaturing agent. Esp. the method is applied to t-PA expressed in E.coli and P. putida. The denaturing agent is pref. arginine, guanidine hydrochloride (both at 0.1-1, esp. 0.25-0.75, mM) or urea, at 0.5-4 (esp. 1-3.5) M in the last stage.
FI872753A 1985-10-23 1987-06-22 Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes FI94050C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FI933868A FI95578C (en) 1985-10-23 1993-09-03 Method for activating genetically engineered heterologous disulfide bridge eukaryotic proteins after expression in prokaryotes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3537708 1985-10-23
DE19853537708 DE3537708A1 (en) 1985-10-23 1985-10-23 METHOD FOR ACTIVATING T-PA AFTER EXPRESSION IN PROKARYONTS
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
EP8600610 1986-10-23

Publications (4)

Publication Number Publication Date
FI872753A FI872753A (en) 1987-06-22
FI872753A0 true FI872753A0 (en) 1987-06-22
FI94050B FI94050B (en) 1995-03-31
FI94050C FI94050C (en) 1995-07-10

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (en) 1985-10-23 1987-06-22 Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes
FI933868A FI95578C (en) 1985-10-23 1993-09-03 Method for activating genetically engineered heterologous disulfide bridge eukaryotic proteins after expression in prokaryotes

Family Applications After (1)

Application Number Title Priority Date Filing Date
FI933868A FI95578C (en) 1985-10-23 1993-09-03 Method for activating genetically engineered heterologous disulfide bridge eukaryotic proteins after expression in prokaryotes

Country Status (26)

Country Link
EP (3) EP0393725B1 (en)
JP (2) JPH0728745B2 (en)
KR (1) KR900009139B1 (en)
AT (2) ATE131489T1 (en)
AU (2) AU590029B2 (en)
CA (1) CA1329157C (en)
CZ (1) CZ280727B6 (en)
DD (1) DD260517A5 (en)
DE (3) DE3537708A1 (en)
DK (2) DK175091B1 (en)
ES (2) ES2061434T3 (en)
FI (2) FI94050C (en)
GR (2) GR920300062T1 (en)
HK (2) HK153496A (en)
HR (1) HRP921075B1 (en)
HU (2) HUT43643A (en)
IE (1) IE62634B1 (en)
IL (1) IL80325A (en)
PT (1) PT83609B (en)
SI (1) SI8611796B (en)
SK (1) SK278317B6 (en)
SU (1) SU1607689A3 (en)
UA (1) UA6023A1 (en)
WO (1) WO1987002673A2 (en)
YU (1) YU47185B (en)
ZA (1) ZA868012B (en)

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US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (en) * 1987-04-28 1988-11-03 Amgen Inc. Method for purifying granulocyte/macrophage colony stimulating factor
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DE3832898A1 (en) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
DE3835350A1 (en) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh ACTIVATION OF GENETICALLY MANUFACTURED ANTIBODY EXPRESSED IN PROKARYONS
DE3903581A1 (en) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE
DE3942143A1 (en) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-PA PRO STABILIZATION
JPH06500084A (en) * 1990-08-20 1994-01-06 ノボ　ノルディスク　アクティーゼルスカブ Biologically active compounds, their production methods and their uses
WO1992004382A1 (en) * 1990-09-05 1992-03-19 Bunge (Australia) Pty. Ltd. Solubilization of proteins in active forms
DE4037196A1 (en) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh METHOD FOR REACTIVATING DENATURED PROTEIN
DE4113750A1 (en) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
DE4139000A1 (en) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh METHOD OF GENERATING BIOLOGICALLY ACTIVE SS-NGF
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
AU3812993A (en) * 1992-03-24 1993-10-21 Synergen, Inc. Refolding and purification of insulin-like growth factor I
SG46612A1 (en) 1992-12-02 1998-02-20 Hoechst Ag Process for obtaining proinsulin in processing correctly linked cystine bridges
DE4405179A1 (en) * 1994-02-18 1995-08-24 Hoechst Ag Method of obtaining insulin with correctly connected cystine bridges
FR2729972B1 (en) * 1995-01-31 1997-04-18 Sanofi Sa PROCESS FOR THE EXTRACTION OF PERIPLASMIC PROTEINS FROM PROKARYOTIC MICROORGANISMS IN THE PRESENCE OF ARGININ
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
ATE260972T1 (en) * 1996-06-11 2004-03-15 Roche Diagnostics Gmbh METHOD FOR ACTIVATING DENATURED PROTEIN
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
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EP1048732A1 (en) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins
EP1077263A1 (en) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins by co-secretion of chaperones
EP1284987B1 (en) 2000-05-16 2007-07-18 Bolder Biotechnology, Inc. Methods for refolding proteins containing free cysteine residues
DE10105911A1 (en) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression of the recombinant proteinase K from Tritirachium album in yeast
DE10105912A1 (en) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
DE102005033250A1 (en) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Process for purifying G-CSF
DE102006009437A1 (en) 2006-03-01 2007-09-13 Bioceuticals Arzneimittel Ag G-CSF liquid formulation
JP5566104B2 (en) 2006-07-14 2014-08-06 ジェネンテック， インコーポレイテッド Refolding of recombinant proteins
WO2008076933A2 (en) 2006-12-14 2008-06-26 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same
EP2547699B1 (en) 2010-03-17 2016-10-19 ratiopharm GmbH Method for obtaining biologically active recombinant human g-csf
AU2011317021B2 (en) * 2010-10-20 2015-07-09 Medimmune, Llc Methods for processing inclusion bodies
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (en) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Methods for the production of polypeptides
CN103852527B (en) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 High-flux protein sample pre-treatment device
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
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Also Published As

Publication number Publication date
FI94050B (en) 1995-03-31
FI933868A (en) 1993-09-03
FI872753A (en) 1987-06-22
ES2020498T3 (en) 1996-04-01
ZA868012B (en) 1987-06-24
GR3018410T3 (en) 1996-03-31
PT83609A (en) 1986-11-01
CZ752686A3 (en) 1996-01-17
DE3537708C2 (en) 1993-07-08
CZ280727B6 (en) 1996-04-17
HRP921075A2 (en) 1995-06-30
JPS62502895A (en) 1987-11-19
ES2061434T3 (en) 1994-12-16
GR920300062T1 (en) 1992-08-31
FI94050C (en) 1995-07-10
SK278317B6 (en) 1996-10-02
IL80325A (en) 1992-06-21
SI8611796B (en) 1998-06-30
HK153596A (en) 1996-08-16
JPH0824594B2 (en) 1996-03-13
DK320387A (en) 1987-06-23
KR870700601A (en) 1987-12-30
WO1987002673A3 (en) 1987-10-22
JPH04218387A (en) 1992-08-07
IE62634B1 (en) 1995-02-22
AU4132189A (en) 1990-01-04
HRP921075B1 (en) 1999-02-28
AU6599386A (en) 1987-05-19
EP0219874B1 (en) 1993-12-15
EP0393725B1 (en) 1995-12-13
FI933868A0 (en) 1993-09-03
DK320387D0 (en) 1987-06-23
WO1987002673A2 (en) 1987-05-07
DK175091B1 (en) 2004-05-24
HU204855B (en) 1992-02-28
CA1329157C (en) 1994-05-03
UA6023A1 (en) 1994-12-29
DK200001897A (en) 2000-12-18
AU607083B2 (en) 1991-02-21
FI95578B (en) 1995-11-15
FI95578C (en) 1996-02-26
YU179686A (en) 1988-06-30
EP0219874A3 (en) 1988-02-10
SK752686A3 (en) 1996-10-01
YU47185B (en) 1995-01-31
IE862683L (en) 1987-04-23
ATE131489T1 (en) 1995-12-15
DE3537708A1 (en) 1987-04-23
EP0219874A2 (en) 1987-04-29
AU590029B2 (en) 1989-10-26
DE3650449D1 (en) 1996-01-25
ATE98648T1 (en) 1994-01-15
DD260517A5 (en) 1988-09-28
DE3689404D1 (en) 1994-01-27
EP0393725A1 (en) 1990-10-24
IL80325A0 (en) 1987-01-30
SI8611796A (en) 1996-10-31
JPH0728745B2 (en) 1995-04-05
EP0253823A1 (en) 1988-01-27
DK175109B1 (en) 2004-06-07
ES2020498A4 (en) 1991-08-16
SU1607689A3 (en) 1990-11-15
HUT43643A (en) 1987-11-30
PT83609B (en) 1988-10-14
HK153496A (en) 1996-08-16
KR900009139B1 (en) 1990-12-22

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Owner name: BOEHRINGER MANNHEIM GMBH

MA Patent expired