HUT40140A - Process for solubilizing and restoring homatropin - Google Patents
Process for solubilizing and restoring homatropinInfo
- Publication number
- HUT40140A HUT40140A HU86741A HU74186A HUT40140A HU T40140 A HUT40140 A HU T40140A HU 86741 A HU86741 A HU 86741A HU 74186 A HU74186 A HU 74186A HU T40140 A HUT40140 A HU T40140A
- Authority
- HU
- Hungary
- Prior art keywords
- pref
- urea
- soln
- dimethylsulphone
- naturation
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- ZTVIKZXZYLEVOL-MCOXGKPRSA-N Homatropine Chemical compound O([C@H]1C[C@H]2CC[C@@H](C1)N2C)C(=O)C(O)C1=CC=CC=C1 ZTVIKZXZYLEVOL-MCOXGKPRSA-N 0.000 title 1
- 229960000857 homatropine Drugs 0.000 title 1
- 230000003381 solubilizing effect Effects 0.000 title 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 4
- 239000004202 carbamide Substances 0.000 abstract 4
- 230000008014 freezing Effects 0.000 abstract 2
- 238000007710 freezing Methods 0.000 abstract 2
- HHVIBTZHLRERCL-UHFFFAOYSA-N sulfonyldimethane Chemical compound CS(C)(=O)=O HHVIBTZHLRERCL-UHFFFAOYSA-N 0.000 abstract 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 abstract 2
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 239000007983 Tris buffer Substances 0.000 abstract 1
- 239000003638 chemical reducing agent Substances 0.000 abstract 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 abstract 1
- 239000007800 oxidant agent Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Method for solubilisation and naturation of somatotropin protein (I) from refractile bodies (RB) of a host cell comprises contacting the RB's with aq. urea or dimethylsulphone soln. at a pH effective for solubilisation. The soln. contg. (I) is then contacted with a mild oxidising agent to form intramolecular disulphide bonds between cysteine residues. - Pref. aq. urea is used, pref. at 1-10M, at above freezing pt. to below 25 deg.C and pH pref. 9-12. Pref. Tris buffer is used for pH control. If (I) is aggregated (oxidised in the refractile bodies, a reducing agent is pref. present, e.g. 2-mercaptoethanol.. Naturation is effected in the urea or dimethylsulphone soln. obtd., partic. in 3-5M aq. urea at pH 9-12 and at above freezing pt. to below 25 deg.C. Oxidn. is e.g. with H2O2 or air.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70434185A | 1985-02-22 | 1985-02-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUT40140A true HUT40140A (en) | 1986-11-28 |
| HU201093B HU201093B (en) | 1990-09-28 |
Family
ID=24829071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU86741A HU201093B (en) | 1985-02-22 | 1986-02-21 | Process for solubilisation and recovery of somatotropin |
Country Status (1)
| Country | Link |
|---|---|
| HU (1) | HU201093B (en) |
-
1986
- 1986-02-21 HU HU86741A patent/HU201093B/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| HU201093B (en) | 1990-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK80986A (en) | PROCEDURE FOR SOLUBILIZING SOMATOTROPIN PROTEINS AND BACKING THEM TO NATURAL CONDITION | |
| HK153596A (en) | Process for activating genetically-engineered heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes | |
| YU229982A (en) | Process for the elimination of nitrogen oxide and sulfuroxides from waste gases | |
| HUT40140A (en) | Process for solubilizing and restoring homatropin | |
| ATE22651T1 (en) | METHOD FOR CLEANING OR. ENRICHMENT OF BIOLOGICALLY ACTIVE PROTEINS AND MEANS SUITABLE FOR THIS. | |
| GR3004917T3 (en) | ||
| EP0288272A3 (en) | Process for selective peptide bond cleavage | |
| EP0176658A3 (en) | Process for the regeneration of a washing solution, which is used for the simultaneous washing of nitrogen oxide and sulphur dioxide from flue gases | |
| DE3680255D1 (en) | D-AMINE ACID OXIDASE AND METHOD FOR INSULATING IT. | |
| GR3006794T3 (en) | ||
| ATE73490T1 (en) | METHOD FOR RECOVERING POLYPEPTIDES LOCATED IN THE PERIPLASM OF YEAST. | |
| DE58901266D1 (en) | METHOD FOR REMOVING PHOSPHORIC HYDROGEN FROM EXHAUST AIR. | |
| SE8600987D0 (en) | PROCEDURE FOR PREPARING A PROTEIN CONCENTRATE FROM WHOLE BLOOD | |
| Kieloch | Steel Losses During Reheating in Combustion Gases | |
| SE8402092D0 (en) | PROCEDURE FOR EXPLORING LOW Sulfur Dioxide Content from Exhaust Gas | |
| Khazan | Ways of improving quality of estimates made during treatment of multifactor experiment results | |
| FI945325A (en) | Method for isolating and purifying G-CSF |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| HPC4 | Succession in title of patentee |
Owner name: MONSANTO TECHNOLOGY LLC, US |
|
| HMM4 | Cancellation of final prot. due to non-payment of fee |