FI933868A0 - Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen - Google Patents

Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen

Info

Publication number
FI933868A0
FI933868A0 FI933868A FI933868A FI933868A0 FI 933868 A0 FI933868 A0 FI 933868A0 FI 933868 A FI933868 A FI 933868A FI 933868 A FI933868 A FI 933868A FI 933868 A0 FI933868 A0 FI 933868A0
Authority
FI
Finland
Prior art keywords
expression
pref
prokaryotic cells
technologist
heterologous
Prior art date
Application number
FI933868A
Other languages
English (en)
Swedish (sv)
Other versions
FI933868A (fi
FI95578B (fi
FI95578C (fi
Inventor
Rainer Rudolph
Stephan Fischer
Ralf Mattes
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of FI933868A0 publication Critical patent/FI933868A0/fi
Publication of FI933868A publication Critical patent/FI933868A/fi
Publication of FI95578B publication Critical patent/FI95578B/fi
Application granted granted Critical
Publication of FI95578C publication Critical patent/FI95578C/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Lubricants (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
FI933868A 1985-10-23 1993-09-03 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen FI95578C (fi)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE3537708 1985-10-23
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells
EP8600610 1986-10-23
FI872753A FI94050C (fi) 1985-10-23 1987-06-22 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen
FI872753 1987-06-22

Publications (4)

Publication Number Publication Date
FI933868A0 true FI933868A0 (fi) 1993-09-03
FI933868A FI933868A (fi) 1993-09-03
FI95578B FI95578B (fi) 1995-11-15
FI95578C FI95578C (fi) 1996-02-26

Family

ID=6284269

Family Applications (2)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (fi) 1985-10-23 1987-06-22 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen
FI933868A FI95578C (fi) 1985-10-23 1993-09-03 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FI872753A FI94050C (fi) 1985-10-23 1987-06-22 Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen

Country Status (26)

Country Link
EP (3) EP0393725B1 (fi)
JP (2) JPH0728745B2 (fi)
KR (1) KR900009139B1 (fi)
AT (2) ATE131489T1 (fi)
AU (2) AU590029B2 (fi)
CA (1) CA1329157C (fi)
CZ (1) CZ280727B6 (fi)
DD (1) DD260517A5 (fi)
DE (3) DE3537708A1 (fi)
DK (2) DK175091B1 (fi)
ES (2) ES2020498T3 (fi)
FI (2) FI94050C (fi)
GR (2) GR920300062T1 (fi)
HK (2) HK153496A (fi)
HR (1) HRP921075B1 (fi)
HU (2) HU204855B (fi)
IE (1) IE62634B1 (fi)
IL (1) IL80325A (fi)
PT (1) PT83609B (fi)
SI (1) SI8611796B (fi)
SK (1) SK278317B6 (fi)
SU (1) SU1607689A3 (fi)
UA (1) UA6023A1 (fi)
WO (1) WO1987002673A2 (fi)
YU (1) YU47185B (fi)
ZA (1) ZA868012B (fi)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
AU621051B2 (en) 1987-04-28 1992-03-05 Amgen, Inc. Method for purifying granulocyte-macrophage colony stimulating factor
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
DE69126434D1 (de) * 1990-08-20 1997-07-10 Novo Nordisk As Prozess für die Herstellung von biologisch aktivem IGF-1 durch Verwendung von amino-terminal verlängertem IGF-1
EP0547102B1 (en) * 1990-09-05 1998-07-08 Southern Cross Biotech Pty.Ltd. Solubilization of proteins in active forms
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
WO1993019084A1 (en) * 1992-03-24 1993-09-30 Synergen, Inc. Refolding and purification of insulin-like growth factor i
DE59305396D1 (de) 1992-12-02 1997-03-20 Hoechst Ag Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
AU714318B2 (en) * 1996-06-11 2000-01-06 Roche Diagnostics Gmbh Process for the activation of denatured protein
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
ATE367398T1 (de) 2000-05-16 2007-08-15 Bolder Biotechnology Inc Verfahren zur rückfaltung von proteinen mit freien cysteinresten
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
WO2008008975A2 (en) 2006-07-14 2008-01-17 Genentech, Inc. Refolding of recombinant proteins
EP2102355B1 (en) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same
EA022821B1 (ru) 2010-03-17 2016-03-31 Ратиофарм Гмбх Способ получения биологически активного рекомбинантного г-ксф человека
CN103168046A (zh) * 2010-10-20 2013-06-19 米迪缪尼有限公司 用于加工包涵体的方法
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
EP3562839A4 (en) 2016-12-30 2020-09-02 Biogend Therapeutics Co., Ltd. RECOMBINANT POLYPEPTIDES, ASSOCIATED COMPOSITIONS AND PROCESSES
AU2021399935A1 (en) 2020-12-18 2023-06-29 Richter Gedeon Nyrt. Methods for the purification of refolded fc-peptide fusion protein

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5135481A (ja) * 1974-09-18 1976-03-25 Fujiwa Kako Kk Kojundohitorokinaaze no seiho
US4468633A (en) 1982-04-28 1984-08-28 The Bendix Corporation Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
AR241654A1 (es) 1982-05-05 1992-10-30 Genentech Inc Procedimiento para producir activador de plasminogeno de tejido humano.
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
GR79124B (fi) * 1982-12-22 1984-10-02 Genentech Inc
WO1984003711A1 (en) * 1983-03-25 1984-09-27 Celltech Ltd A process for the production of a protein
JPS6051119A (ja) * 1983-08-30 1985-03-22 Green Cross Corp:The ウロキナ−ゼ乾燥製剤
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) * 1986-04-01 1989-02-17 Sotralentz Sa Conteneur sur palette avec dispositif de protection en treillis plie et renforce
JPH0651119A (ja) * 1992-07-28 1994-02-25 Sekisui Chem Co Ltd 位相差板の製造方法

Also Published As

Publication number Publication date
DK200001897A (da) 2000-12-18
DD260517A5 (de) 1988-09-28
CZ752686A3 (en) 1996-01-17
DE3537708C2 (fi) 1993-07-08
DK320387D0 (da) 1987-06-23
EP0393725B1 (de) 1995-12-13
SK752686A3 (en) 1996-10-01
SI8611796A (sl) 1996-10-31
HRP921075B1 (en) 1999-02-28
ES2020498T3 (es) 1996-04-01
FI872753A (fi) 1987-06-22
JPH04218387A (ja) 1992-08-07
FI933868A (fi) 1993-09-03
DK320387A (da) 1987-06-23
AU590029B2 (en) 1989-10-26
FI94050C (fi) 1995-07-10
ATE98648T1 (de) 1994-01-15
DE3537708A1 (de) 1987-04-23
DK175091B1 (da) 2004-05-24
PT83609B (pt) 1988-10-14
IL80325A0 (en) 1987-01-30
EP0219874B1 (de) 1993-12-15
EP0219874A2 (de) 1987-04-29
WO1987002673A3 (fr) 1987-10-22
AU4132189A (en) 1990-01-04
KR900009139B1 (ko) 1990-12-22
HUT43643A (en) 1987-11-30
IE862683L (en) 1987-04-23
ES2020498A4 (es) 1991-08-16
EP0393725A1 (de) 1990-10-24
KR870700601A (ko) 1987-12-30
IL80325A (en) 1992-06-21
AU6599386A (en) 1987-05-19
SI8611796B (sl) 1998-06-30
WO1987002673A2 (en) 1987-05-07
YU47185B (sh) 1995-01-31
GR920300062T1 (en) 1992-08-31
GR3018410T3 (en) 1996-03-31
DE3689404D1 (de) 1994-01-27
CA1329157C (en) 1994-05-03
JPH0824594B2 (ja) 1996-03-13
JPS62502895A (ja) 1987-11-19
HU204855B (en) 1992-02-28
UA6023A1 (uk) 1994-12-29
YU179686A (en) 1988-06-30
IE62634B1 (en) 1995-02-22
JPH0728745B2 (ja) 1995-04-05
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
PT83609A (de) 1986-11-01
HK153596A (en) 1996-08-16
DE3650449D1 (de) 1996-01-25
DK175109B1 (da) 2004-06-07
ES2061434T3 (es) 1994-12-16
EP0253823A1 (de) 1988-01-27
ATE131489T1 (de) 1995-12-15
EP0219874A3 (en) 1988-02-10
CZ280727B6 (cs) 1996-04-17
ZA868012B (en) 1987-06-24
HK153496A (en) 1996-08-16
SK278317B6 (en) 1996-10-02
FI94050B (fi) 1995-03-31
AU607083B2 (en) 1991-02-21
FI872753A0 (fi) 1987-06-22
SU1607689A3 (ru) 1990-11-15
HRP921075A2 (en) 1995-06-30

Similar Documents

Publication Publication Date Title
FI933868A0 (fi) Menetelmä geeniteknisesti valmistettujen, heterologisten, disulfidisiltoja käsittävien eukarioottisten proteiinien aktivoimiseksi prokariooteissa ilmentämisen jälkeen
AU605124B2 (en) Novel peptide plasminogen activators
IE831024L (en) Human tissue plasminogen activator
Ichio et al. Glutathione reductase in the sea urchin egg. I. Purification and general properties
De Toledo et al. DNA strand scission in E. coli by electronically excited state molecules generated by enzymatic systems
Khesin et al. Ribonuclease activity in preparations of human leukocyte interferon
Yoo Protein degradation in Euglena gracilis: Purification and characterization of the major proteinase.

Legal Events

Date Code Title Description
BB Publication of examined application
MA Patent expired