DE69934585D1 - Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck - Google Patents

Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck

Info

Publication number
DE69934585D1
DE69934585D1 DE69934585T DE69934585T DE69934585D1 DE 69934585 D1 DE69934585 D1 DE 69934585D1 DE 69934585 T DE69934585 T DE 69934585T DE 69934585 T DE69934585 T DE 69934585T DE 69934585 D1 DE69934585 D1 DE 69934585D1
Authority
DE
Germany
Prior art keywords
kbar
refolding
solution
aggregates
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69934585T
Other languages
English (en)
Other versions
DE69934585T2 (de
Inventor
W Randolph
F Carpenter
John Richard St
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BaroFold Inc
Original Assignee
BaroFold Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22232166&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69934585(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by BaroFold Inc filed Critical BaroFold Inc
Publication of DE69934585D1 publication Critical patent/DE69934585D1/de
Application granted granted Critical
Publication of DE69934585T2 publication Critical patent/DE69934585T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
DE69934585T 1998-07-09 1999-07-09 Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck Expired - Lifetime DE69934585T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9220898P 1998-07-09 1998-07-09
US92208P 1998-07-09
PCT/US1999/015419 WO2000002901A1 (en) 1998-07-09 1999-07-09 High pressure refolding of protein aggregates and inclusion bodies

Publications (2)

Publication Number Publication Date
DE69934585D1 true DE69934585D1 (de) 2007-02-08
DE69934585T2 DE69934585T2 (de) 2007-10-25

Family

ID=22232166

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69934585T Expired - Lifetime DE69934585T2 (de) 1998-07-09 1999-07-09 Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck

Country Status (7)

Country Link
US (5) US6489450B2 (de)
EP (1) EP1095056B1 (de)
AT (1) ATE349460T1 (de)
AU (1) AU4975699A (de)
DE (1) DE69934585T2 (de)
DK (1) DK1095056T3 (de)
WO (1) WO2000002901A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000002901A1 (en) 1998-07-09 2000-01-20 University Technology Corporation High pressure refolding of protein aggregates and inclusion bodies
AU6347100A (en) * 1999-07-16 2001-02-05 Human Genome Sciences, Inc. Real-time, in situ biomanufacturing process monitoring and control in response to ir spectroscopy
US7615617B2 (en) * 1999-10-25 2009-11-10 University Of Delaware Use of hydrostatic pressure to inhibit and reverse protein aggregation and facilitate protein refolding
MXPA03003867A (es) 2000-10-31 2004-10-15 Univ Colorado Regents Desagregacion mejorada de proteina y replegamiento utilizando alta presion.
US8815557B2 (en) * 2005-07-08 2014-08-26 University Of Chicago Compositions and methods for refolding of denatured proteins
US20070141341A1 (en) * 2005-12-15 2007-06-21 Dewald Aaron Coating containing denatured aloe vera, for covering either chlorine-generating compositions or other hazardous chemicals and method for making
GB0605684D0 (en) * 2006-03-21 2006-05-03 Sicor Biotech Uab Method For Purifying Granulocyte-Colony Stimulating Factor
WO2008033555A2 (en) * 2006-09-15 2008-03-20 Barofold, Inc. High-pressure refolding of difficult-to-fold proteins
EP2059523A2 (de) * 2006-09-15 2009-05-20 Barofold, Inc. Hochdruckbehandlung von proteinen für reduzierte immunogenizität
US20080249286A1 (en) * 2007-04-05 2008-10-09 Seefeldt Matthew B High-pressure refolding of proteins in the presence of binding partners
US7829681B2 (en) * 2007-04-05 2010-11-09 Barofold Inc. High-pressure inclusion body solubilization and protease clipping of recombinant fusion proteins
CA2689145A1 (en) * 2007-06-04 2008-12-11 Pressure Biosciences Inc. Pressure-enhanced extraction and partitioning of molecules
US20090076247A1 (en) * 2007-09-10 2009-03-19 Seefeldt Matthew B High pressure refolding of monoclonal antibody aggregates
US8273561B2 (en) * 2007-10-05 2012-09-25 Nuron Biotech, Inc. High pressure treatment of aggregated interferons
US20090286301A1 (en) * 2008-05-07 2009-11-19 Pressure Biosciences Inc. Extraction of biomolecular complexes assisted by alternating hydrostatic pressure
US20100112660A1 (en) * 2008-05-30 2010-05-06 Barofold, Inc. Method for Derivatization of Proteins Using Hydrostatic Pressure
WO2012012388A2 (en) 2010-07-19 2012-01-26 Barofold, Inc. Therapeutic protein compositions having reduced immunogenicity and/or improved efficacy
CA2808412C (en) 2010-08-18 2021-10-12 Pressure Biosciences Inc. Flow-through high hydrostatic pressure microfluidic sample preparation device and related methods therefor
WO2012058412A1 (en) 2010-10-28 2012-05-03 Ting Edmund Y System and method for microplate pressurization
BR112014013369A2 (pt) * 2011-12-01 2017-06-13 Barofold Inc métodos e sistemas para redobramento de proteína
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
ZA201501696B (en) 2012-08-30 2016-01-27 Merial Ltd Hyperbaric device and methods for producing inactivated vaccines and for refolding/solubilizing recombinant proteins
EP2890392B1 (de) 2012-08-30 2019-04-17 Merial, Inc. Expression des chimären proteins ksac und verfahren zur herstellung löslicher proteine durch hochdruck
WO2021050009A2 (en) * 2019-09-13 2021-03-18 National Science And Technology Development Agency Composition of renaturation buffer solution for dimeric proteins and method of renaturation dimeric proteins using the composition thereof
US20240101598A1 (en) 2020-12-18 2024-03-28 Richter Gedeon Nyrt. Methods for the purification of refolded fc-peptide fusion protein
CN113683676A (zh) * 2021-08-27 2021-11-23 恒敬合创生物医药(上海)有限公司 一种环保的可逆的蛋白变性工艺

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234620A (en) * 1978-12-26 1980-11-18 A. E. Staley Manufacturing Company Water-soluble vegetable protein aggregates
US4652630A (en) 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US4659568A (en) 1985-02-27 1987-04-21 American Cyanamid Company Process for solubilization, purification and characterization of protein from insoluble protein aggregates or complexes and compositions of matter therefrom
US4766224A (en) 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4677196A (en) 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US5453363A (en) 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
US4929700A (en) 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
US5162507A (en) 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4985544A (en) 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
US4923967A (en) 1988-09-26 1990-05-08 Eli Lilly And Company Purification and refolding of recombinant proteins
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5064943A (en) 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
GB8927008D0 (en) 1989-11-29 1990-01-17 Ciba Geigy Novel process for the production of unfused protein in e.coli
US5109117A (en) 1990-06-26 1992-04-28 Monsanto Company Method of somatotropin naturation using urea and a soluble organic alcohol
US5023323A (en) 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
US5288931A (en) 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
US5288462A (en) * 1992-05-18 1994-02-22 Stephen D. Carter Sterilization apparatus and method
EP0648199B1 (de) * 1992-06-29 1998-03-11 United Technologies Corporation Verwendung energetischen Abfallmaterials für Sprengstoffe
US5605691A (en) 1992-09-17 1997-02-25 Ophidian Pharmaceuticals, Inc. Immunologically active proteins from inclusion bodies
US5714371A (en) 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
US5800811A (en) * 1995-06-06 1998-09-01 Hall; Frederick L. Artificial skin prepared from coclagen matrix containing transforming growth factor-β having a collagen binding site
ES2165075T3 (es) * 1996-07-02 2002-03-01 Bbi Bioseq Inc Union por presion de complejos biomoleculares.
WO2000002901A1 (en) 1998-07-09 2000-01-20 University Technology Corporation High pressure refolding of protein aggregates and inclusion bodies
US7615617B2 (en) 1999-10-25 2009-11-10 University Of Delaware Use of hydrostatic pressure to inhibit and reverse protein aggregation and facilitate protein refolding
MXPA03003867A (es) 2000-10-31 2004-10-15 Univ Colorado Regents Desagregacion mejorada de proteina y replegamiento utilizando alta presion.

Also Published As

Publication number Publication date
EP1095056A1 (de) 2001-05-02
US20020137157A1 (en) 2002-09-26
US20110046357A1 (en) 2011-02-24
US6489450B2 (en) 2002-12-03
US20030083475A1 (en) 2003-05-01
ATE349460T1 (de) 2007-01-15
DK1095056T3 (da) 2007-04-23
WO2000002901A1 (en) 2000-01-20
US20060188970A1 (en) 2006-08-24
US20100255536A1 (en) 2010-10-07
US8710197B2 (en) 2014-04-29
AU4975699A (en) 2000-02-01
US7064192B2 (en) 2006-06-20
US7767795B2 (en) 2010-08-03
DE69934585T2 (de) 2007-10-25
EP1095056B1 (de) 2006-12-27

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