ES2302542T3 - Procedimiento para unir secuencias de interes. - Google Patents

Procedimiento para unir secuencias de interes. Download PDF

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Publication number
ES2302542T3
ES2302542T3 ES04762853T ES04762853T ES2302542T3 ES 2302542 T3 ES2302542 T3 ES 2302542T3 ES 04762853 T ES04762853 T ES 04762853T ES 04762853 T ES04762853 T ES 04762853T ES 2302542 T3 ES2302542 T3 ES 2302542T3
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Spain
Prior art keywords
extension
variable region
interest
procedure
sequences
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Martin B Oleksiewicz
Lars S Nielsen
Peter S Andersen
Margit H Hansen
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Symphogen AS
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Symphogen AS
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00Drugs for disorders of the muscular or neuromuscular system
    • A61P21/02Muscle relaxants, e.g. for tetanus or cramps
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Public Health (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Pain & Pain Management (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Neurology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un procedimiento de unión de una pluralidad de secuencias de nucleótidos no contiguas de interés, comprendiendo dicho procedimiento: a) amplificar, en un procedimiento de amplificación molecular múltiplex, secuencias de nucleótidos de interés usando un molde derivado de una célula única aislada; y b) efectuar la unión de las secuencias de nucleótidos de interés amplificadas en la etapa a), en el que las secuencias de nucleótidos de interés comprenden secuencias que codifican la región variable y la unión genera un par análogo de secuencias que codifican la región variable.
ES04762853T 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interes. Expired - Lifetime ES2302542T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US50445503P 2003-09-18 2003-09-18
US50458903P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15

Publications (1)

Publication Number Publication Date
ES2302542T3 true ES2302542T3 (es) 2008-07-16

Family

ID=44209637

Family Applications (2)

Application Number Title Priority Date Filing Date
ES04762853T Expired - Lifetime ES2302542T3 (es) 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interes.
ES08152066T Expired - Lifetime ES2375529T3 (es) 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interés.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES08152066T Expired - Lifetime ES2375529T3 (es) 2003-09-18 2004-09-17 Procedimiento para unir secuencias de interés.

Country Status (18)

Country Link
US (2) US7749697B2 (es)
EP (3) EP1516929A3 (es)
JP (1) JP4651619B2 (es)
CN (2) CN102199593A (es)
AT (2) ATE388228T1 (es)
AU (1) AU2004286019B2 (es)
BR (1) BRPI0414282A (es)
CA (1) CA2539576A1 (es)
DE (1) DE602004012299T2 (es)
DK (2) DK1670912T3 (es)
ES (2) ES2302542T3 (es)
MY (1) MY148907A (es)
NZ (1) NZ545936A (es)
PL (2) PL1670912T3 (es)
PT (2) PT1921144E (es)
SI (2) SI1670912T1 (es)
TW (1) TWI333977B (es)
WO (1) WO2005042774A2 (es)

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