BRPI0618391A2 - método para identificar um nucleotìdeo individual, método para seqüenciar uma seqüência de ácido nucleico alvo, e, kit para seqüenciar um ácido nucleico - Google Patents

método para identificar um nucleotìdeo individual, método para seqüenciar uma seqüência de ácido nucleico alvo, e, kit para seqüenciar um ácido nucleico Download PDF

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Publication number
BRPI0618391A2
BRPI0618391A2 BRPI0618391-3A BRPI0618391A BRPI0618391A2 BR PI0618391 A2 BRPI0618391 A2 BR PI0618391A2 BR PI0618391 A BRPI0618391 A BR PI0618391A BR PI0618391 A2 BRPI0618391 A2 BR PI0618391A2
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BR
Brazil
Prior art keywords
nucleic acid
sequencing
identifying
nucleotide
kit
Prior art date
Application number
BRPI0618391-3A
Other languages
English (en)
Inventor
Hagan Bayley
Yann Astier
Orit Braha
Original Assignee
Isis Innovation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation filed Critical Isis Innovation
Publication of BRPI0618391A2 publication Critical patent/BRPI0618391A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Abstract

MéTODO PARA IDENTIFICAR UM NUCLEOTìDEO INDIVIDUAL, MéTODO PARA SEQUENCIAR UMA SEQUêNCIA DE áCIDO NUCLEICO ALVO, E, KIT PARA SEQUENCIAR UM áCIDO NUCLEICO. A invenção refere-se a um método de identificação de um nucleotídeo individual, compreendendo (a) contatar o nueleotídeo com um poro de proteína de transmembrana de modo que o nucleotídeo interaja com o poro e (b) medir a corrente passando através do poro durante a interação e deste modo determinar a identidade do nucleotídeo. A invenção também se refere a um método de seqilenciamento de sequências de ácido nucleico e aos kits relacionados com o mesmo.
BRPI0618391-3A 2005-11-15 2006-11-15 método para identificar um nucleotìdeo individual, método para seqüenciar uma seqüência de ácido nucleico alvo, e, kit para seqüenciar um ácido nucleico BRPI0618391A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0523282.2A GB0523282D0 (en) 2005-11-15 2005-11-15 Methods using pores
PCT/GB2006/004265 WO2007057668A1 (en) 2005-11-15 2006-11-15 Methods using pores

Publications (1)

Publication Number Publication Date
BRPI0618391A2 true BRPI0618391A2 (pt) 2011-08-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0618391-3A BRPI0618391A2 (pt) 2005-11-15 2006-11-15 método para identificar um nucleotìdeo individual, método para seqüenciar uma seqüência de ácido nucleico alvo, e, kit para seqüenciar um ácido nucleico

Country Status (16)

Country Link
US (2) US8105846B2 (pt)
EP (2) EP2272981B1 (pt)
JP (1) JP5328362B2 (pt)
KR (1) KR20080083281A (pt)
CN (1) CN101356288B (pt)
AT (1) ATE545712T1 (pt)
AU (1) AU2006314296B2 (pt)
BR (1) BRPI0618391A2 (pt)
CA (1) CA2629009C (pt)
GB (1) GB0523282D0 (pt)
IL (1) IL191319A (pt)
MY (1) MY149161A (pt)
NZ (1) NZ568163A (pt)
RU (1) RU2008123842A (pt)
UA (1) UA95087C2 (pt)
WO (1) WO2007057668A1 (pt)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US9121843B2 (en) 2007-05-08 2015-09-01 Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
US20110229877A1 (en) * 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) * 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
EP2342359B1 (en) * 2008-08-01 2015-03-04 Brown University System and methods for determining molecules using mass spectrometry and related techniques
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
WO2010062903A2 (en) * 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
US9222082B2 (en) 2009-01-30 2015-12-29 Oxford Nanopore Technologies Limited Hybridization linkers
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
US9017937B1 (en) 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
EP2483680A4 (en) 2009-09-30 2014-01-01 Quantapore Inc ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE
WO2011067559A1 (en) 2009-12-01 2011-06-09 Oxford Nanopore Technologies Limited Biochemical analysis instrument
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
GB2490846A (en) * 2010-03-05 2012-11-14 Univ Utah Res Found Detection of nucleic acid lesions and adducts using nanopores
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
EP2622343B1 (en) 2010-10-01 2016-01-20 Oxford Nanopore Technologies Limited Biochemical analysis apparatus using nanopores
US8673757B2 (en) 2010-10-28 2014-03-18 International Business Machines Corporation Structure and method for using high-k material as an etch stop layer in dual stress layer process
WO2012107778A2 (en) 2011-02-11 2012-08-16 Oxford Nanopore Technologies Limited Mutant pores
EP3633370A1 (en) 2011-05-27 2020-04-08 Oxford Nanopore Technologies Limited Coupling method
RU2467218C1 (ru) * 2011-06-16 2012-11-20 Закрытое Акционерное Общество "Научно-производственное объединение "Гидравлические аппараты" (ЗАО "НПО "Гидроаппарат") Гидромуфта
CA2944478A1 (en) 2011-07-20 2013-01-24 The Regents Of The University Of California Dual-pore device
IN2014DN00221A (pt) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
CN104126018B (zh) 2011-12-29 2021-09-14 牛津纳米孔技术公司 酶方法
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
MY175528A (en) * 2012-04-02 2020-07-01 Lux Bio Group Inc Apparatus and method for molecular separation, purification, and sensing
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
WO2014013259A1 (en) * 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
CA2879355C (en) * 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
EP2917366B1 (en) 2012-11-06 2017-08-02 Oxford Nanopore Technologies Limited Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103267785B (zh) * 2013-05-14 2016-04-13 西北大学 一种检测药物包裹或释放过程的单分子分析方法
US9862997B2 (en) 2013-05-24 2018-01-09 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed FRET detection
AU2014270410B2 (en) 2013-05-24 2020-07-16 Illumina Cambridge Limited Pyrophosphorolytic sequencing
GB201316849D0 (en) 2013-09-23 2013-11-06 Isis Innovation Method
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US10385389B2 (en) 2014-01-22 2019-08-20 Oxford Nanopore Technologies Ltd. Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
GB201411285D0 (en) 2014-06-25 2014-08-06 Prosser Joseph Sequencer
CN117164682A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
AU2015330841B2 (en) 2014-10-10 2019-08-15 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
US10480026B2 (en) 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
CA2964790C (en) 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
WO2016069806A2 (en) 2014-10-31 2016-05-06 Genia Technologies, Inc. Alpha-hemolysin variants with altered characteristics
US10060903B2 (en) * 2014-11-05 2018-08-28 Genia Technologies, Inc. Exporting measurements of nanopore arrays
US9863904B2 (en) 2014-12-19 2018-01-09 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
US9557294B2 (en) 2014-12-19 2017-01-31 Genia Technologies, Inc. Nanopore-based sequencing with varying voltage stimulus
WO2016126748A1 (en) * 2015-02-02 2016-08-11 Two Pore Guys, Inc. Labile linkers for biomarker detection
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) * 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
CN108137656A (zh) 2015-09-24 2018-06-08 豪夫迈·罗氏有限公司 α-溶血素变体
US10126262B2 (en) 2015-09-24 2018-11-13 Genia Technologies, Inc. Differential output of analog memories storing nanopore measurement samples
CN105259229B (zh) * 2015-10-22 2018-04-20 清华大学 一种检测药物的单分子分析方法
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
EP3387432B1 (en) 2015-12-08 2022-09-28 Katholieke Universiteit Leuven KU Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
JP6579948B2 (ja) * 2015-12-24 2019-09-25 株式会社日立ハイテクノロジーズ 生体ポリマを分析するための測定試薬及び分析デバイス
EP4015531A3 (en) 2016-03-02 2022-09-28 Oxford Nanopore Technologies PLC Mutant pore
CA3212147A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Plc Mutant pore
EP3464616B1 (en) * 2016-05-25 2022-05-04 Oxford Nanopore Technologies plc Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2018009346A1 (en) 2016-07-05 2018-01-11 Quantapore, Inc. Optically based nanopore sequencing
WO2018146491A1 (en) * 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
EP4026916B1 (en) * 2017-04-19 2023-10-18 Electronic Biosciences Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
SG11201913174PA (en) 2017-06-30 2020-01-30 Vib Vzw Novel protein pores
JP7042910B2 (ja) * 2017-11-27 2022-03-28 エフ．ホフマン－ラ ロシュ アーゲー ナノポア－ｓｂｓ信号のための規格化およびベースラインシフト除去
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB202202716D0 (en) 2022-02-28 2022-04-13 Oxford Nanopore Tech Plc Apparatus and methods for controlling insertion of a membrane channel into a membrane

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
EP0753071A1 (en) 1993-04-28 1997-01-15 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1999005167A1 (en) * 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6267872B1 (en) * 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1192103A1 (en) 1999-06-22 2002-04-03 President And Fellows of Harvard College Control of solid state dimensional features
WO2001002425A2 (en) 1999-06-29 2001-01-11 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
AU1804001A (en) 1999-12-02 2001-06-12 Molecular Staging, Inc. Generation of single-strand circular dna from linear self-annealing segments
EP2083015B1 (en) 2000-02-11 2016-04-06 The Texas A & M University System Biosensor compositions and methods of use
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
CA2448289A1 (en) * 2001-05-29 2002-12-19 Asahi Glass Company, Limited Method of constructing host and method of producing heterologous protein
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
US20030215881A1 (en) 2002-05-10 2003-11-20 Hagan Bayley Stochastic sensing through covalent interactions
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
AU2005272823B2 (en) 2004-08-13 2012-04-12 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore DNA readout platform
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP1963530B1 (en) 2005-12-22 2011-07-27 Pacific Biosciences of California, Inc. Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
US20100311602A1 (en) 2006-10-13 2010-12-09 J. Craig Venter Institute, Inc. Sequencing method
WO2008102121A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
EP3798317B1 (en) 2007-04-04 2024-01-03 The Regents of the University of California Compositions, devices, systems, and methods for using a nanopore
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
EP3425060B1 (en) 2008-03-28 2021-10-27 Pacific Biosciences of California, Inc. Compositions and methods for nucleic acid sequencing
JP2011527191A (ja) * 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
EP3029467B1 (en) 2008-09-22 2020-01-08 University of Washington Msp nanopores and related methods

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Publication number Publication date
US8785211B2 (en) 2014-07-22
CA2629009C (en) 2016-02-02
IL191319A (en) 2011-10-31
CN101356288B (zh) 2016-02-03
KR20080083281A (ko) 2008-09-17
AU2006314296B2 (en) 2012-08-23
ATE545712T1 (de) 2012-03-15
CA2629009A1 (en) 2007-05-24
WO2007057668A1 (en) 2007-05-24
US20080311582A1 (en) 2008-12-18
MY149161A (en) 2013-07-31
EP1951898B1 (en) 2012-02-15
RU2008123842A (ru) 2009-12-27
JP2009519705A (ja) 2009-05-21
EP2272981A1 (en) 2011-01-12
EP1951898A1 (en) 2008-08-06
US20120219960A1 (en) 2012-08-30
AU2006314296A1 (en) 2007-05-24
NZ568163A (en) 2011-11-25
CN101356288A (zh) 2009-01-28
EP2272981B1 (en) 2013-01-09
JP5328362B2 (ja) 2013-10-30
GB0523282D0 (en) 2005-12-21
UA95087C2 (en) 2011-07-11
US8105846B2 (en) 2012-01-31

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