BR112018073378A2 - composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos - Google Patents

composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos

Info

Publication number
BR112018073378A2
BR112018073378A2 BR112018073378-4A BR112018073378A BR112018073378A2 BR 112018073378 A2 BR112018073378 A2 BR 112018073378A2 BR 112018073378 A BR112018073378 A BR 112018073378A BR 112018073378 A2 BR112018073378 A2 BR 112018073378A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
acid sequencing
methods
labeled nucleotide
nucleotide compositions
Prior art date
Application number
BR112018073378-4A
Other languages
English (en)
Inventor
M. Rothberg Jonathan
Lackey Jeremy
Reed Brian
Shi Xinghua
Huang Haidong
Dodd David
Original Assignee
Quantum-Si Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum-Si Incorporated filed Critical Quantum-Si Incorporated
Publication of BR112018073378A2 publication Critical patent/BR112018073378A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/103—Nucleic acid detection characterized by the use of physical, structural and functional properties luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Medicinal Preparation (AREA)

Abstract

a presente invenção refere-se a métodos de sequenciamento de moléculas com base em tempos de vida e/ou intensidades de luminescência. em alguns aspectos, métodos de sequenciamento de ácidos nucleicos envolvem determinar os tempos de vida de luminescência e, opcionalmente, intensidades de luminescência, de uma série de nucleotídeos marcados de forma luminescente incorporados durante uma reação de sequenciamento de ácido nucleico. em alguns aspectos, a invenção fornece composições que compreendem nucleotídeos marcados de forma luminescente.
BR112018073378-4A 2016-05-20 2017-05-20 composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos BR112018073378A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662339790P 2016-05-20 2016-05-20
US62/339,790 2016-05-20
US201662426144P 2016-11-23 2016-11-23
US62/426,144 2016-11-23
PCT/US2017/033706 WO2017201514A1 (en) 2016-05-20 2017-05-20 Labeled nucleotide compositions and methods for nucleic acid sequencing

Publications (1)

Publication Number Publication Date
BR112018073378A2 true BR112018073378A2 (pt) 2019-03-06

Family

ID=59021574

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018073378-4A BR112018073378A2 (pt) 2016-05-20 2017-05-20 composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos

Country Status (11)

Country Link
US (2) US20170362651A1 (pt)
EP (2) EP4428528A2 (pt)
JP (2) JP7133473B2 (pt)
KR (1) KR102425463B1 (pt)
CN (1) CN109328192A (pt)
AU (2) AU2017268466C1 (pt)
BR (1) BR112018073378A2 (pt)
CA (1) CA3025031A1 (pt)
MX (1) MX2018014163A (pt)
TW (1) TW201742922A (pt)
WO (1) WO2017201514A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019012414A2 (pt) 2016-12-19 2020-02-27 Quantum-Si Incorporated Carregamento de moléculas em poços de amostra para análise
WO2018204810A1 (en) 2017-05-05 2018-11-08 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
CN111148850A (zh) 2017-07-24 2020-05-12 宽腾矽公司 高强度经标记的反应物组合物及用于测序的方法
CA3127102A1 (en) 2019-01-23 2020-07-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
JP2022551523A (ja) 2019-10-11 2022-12-09 クアンタム－エスアイ インコーポレイテッド 気相における表面修飾
CA3196155A1 (en) * 2020-09-21 2022-03-24 Quantum-Si Incorporated Methods to minimize photodamage during nucleic acid and peptide sequencing
GB2599729A (en) * 2020-10-12 2022-04-13 Sumitomo Chemical Co Method comprising light emitting marker
WO2024110088A1 (en) * 2022-11-22 2024-05-30 Leica Microsystems Cms Gmbh Method and marker for analyzing a sample

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Also Published As

Publication number Publication date
KR102425463B1 (ko) 2022-07-27
MX2018014163A (es) 2019-04-01
US20170362651A1 (en) 2017-12-21
TW201742922A (zh) 2017-12-16
EP3458465B1 (en) 2024-05-08
AU2017268466B2 (en) 2021-10-07
AU2021290387B2 (en) 2024-02-01
AU2021290387A1 (en) 2022-02-03
JP2022126734A (ja) 2022-08-30
CA3025031A1 (en) 2017-11-23
AU2017268466C1 (en) 2022-04-07
US20240035081A1 (en) 2024-02-01
EP4428528A2 (en) 2024-09-11
JP2019523756A (ja) 2019-08-29
AU2017268466A1 (en) 2018-11-29
CN109328192A (zh) 2019-02-12
JP7133473B2 (ja) 2022-09-08
KR20190007495A (ko) 2019-01-22
JP7519157B2 (ja) 2024-07-19
WO2017201514A1 (en) 2017-11-23
EP3458465A1 (en) 2019-03-27

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B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements