BR112017024573A2 - método de determinação da sequência de um ácido nucleico nuclear usando luminescência resolvida no tempo - Google Patents

método de determinação da sequência de um ácido nucleico nuclear usando luminescência resolvida no tempo

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Publication number
BR112017024573A2
BR112017024573A2 BR112017024573A BR112017024573A BR112017024573A2 BR 112017024573 A2 BR112017024573 A2 BR 112017024573A2 BR 112017024573 A BR112017024573 A BR 112017024573A BR 112017024573 A BR112017024573 A BR 112017024573A BR 112017024573 A2 BR112017024573 A2 BR 112017024573A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
determining
acid sequence
time
luminescence
Prior art date
Application number
BR112017024573A
Other languages
English (en)
Other versions
BR112017024573B1 (pt
Inventor
M. Rothberg Jonathan
Lackey Jeremy
Reed Brian
Shi Xinghua
Huang Haidong
Original Assignee
Quantum-Si Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/821,688 external-priority patent/US9885657B2/en
Application filed by Quantum-Si Incorporated filed Critical Quantum-Si Incorporated
Publication of BR112017024573A2 publication Critical patent/BR112017024573A2/pt
Publication of BR112017024573B1 publication Critical patent/BR112017024573B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/064—Stray light conditioning

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

a presente invenção refere-se a métodos de sequenciamento de moléculas com base em tempos de vida e/ou intensidades de luminescência. em alguns aspectos, métodos de sequenciamento de ácidos nucleicos envolvem determinação dos tempos de vida de luminescência e, opcionalmente, intensidades de luminescência, de uma série de nucleotídeos luminescentemente marcados incorporados durante uma reação de sequenciamento de ácido nucleico.
BR112017024573-6A 2015-05-20 2016-05-20 Método de identificação de uma molécula única em uma amostra BR112017024573B1 (pt)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US201562164464P 2015-05-20 2015-05-20
US201562164482P 2015-05-20 2015-05-20
US201562164506P 2015-05-20 2015-05-20
US201562164485P 2015-05-20 2015-05-20
US62/164,506 2015-05-20
US62/164,485 2015-05-20
US62/164,482 2015-05-20
US62/164,464 2015-05-20
US14/821,688 2015-08-07
US14/821,688 US9885657B2 (en) 2014-08-08 2015-08-07 Integrated device with external light source for probing detecting and analyzing molecules
PCT/US2016/033616 WO2016187580A1 (en) 2015-05-20 2016-05-20 Method of determining the sequence of a nuclear acid using time resolved luminescence

Publications (2)

Publication Number Publication Date
BR112017024573A2 true BR112017024573A2 (pt) 2018-07-31
BR112017024573B1 BR112017024573B1 (pt) 2024-03-05

Family

ID=57320962

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017024573-6A BR112017024573B1 (pt) 2015-05-20 2016-05-20 Método de identificação de uma molécula única em uma amostra

Country Status (11)

Country Link
EP (2) EP3298389B1 (pt)
JP (2) JP6936736B2 (pt)
KR (1) KR20180008779A (pt)
CN (1) CN108027325A (pt)
AU (2) AU2016264753B2 (pt)
BR (1) BR112017024573B1 (pt)
CA (1) CA2986237A1 (pt)
HK (1) HK1252483A1 (pt)
MX (2) MX2017014821A (pt)
TW (1) TWI704229B (pt)
WO (1) WO2016187580A1 (pt)

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RS62437B1 (sr) 2015-10-25 2021-11-30 Sanofi Sa Trispecifični i/ili trivalentni vezujući proteini za prevenciju ili lečenje hiv infekcije
WO2017143129A1 (en) 2016-02-17 2017-08-24 Tesseract Health, Inc. Sensor and device for lifetime imaging and detection applications
EP4257193A3 (en) 2016-04-13 2024-01-24 Sanofi Trispecific and/or trivalent binding proteins
CA3047122A1 (en) * 2016-12-16 2018-06-21 Quantum-Si Incorporated Optical coupler and waveguide system
MX2019007530A (es) 2016-12-22 2019-08-16 Quantum Si Inc Fotodetector integrado con agrupamiento de pixeles directo.
AU2018261007A1 (en) 2017-05-05 2019-11-07 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
WO2018210394A1 (en) * 2017-05-19 2018-11-22 Københavns Universitet Coherent single photon source
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AU2018307742A1 (en) * 2017-07-24 2020-01-30 Quantum-Si Incorporated Optical rejection photonic structures
EP3658682A4 (en) * 2017-07-24 2021-06-02 Quantum-si Incorporated HIGH INTENSITY LABEL REAGENT COMPOSITIONS AND SEQUENCING METHODS
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BR112021003146A2 (pt) * 2018-08-29 2021-05-11 Quantum-Si Incorporated sistema e métodos para detectar o tempo de vida ao usar fotodetectores de contagem de fótons
CN111333605B (zh) * 2018-12-18 2022-07-15 中国科学院大连化学物理研究所 一种高亮度、高稳定性的细胞膜碳酸酐酶ⅸ荧光探针
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US11613772B2 (en) 2019-01-23 2023-03-28 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
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CN110066723A (zh) * 2019-05-05 2019-07-30 京东方科技集团股份有限公司 基因测序芯片、设备、制造方法
BR112022006682A2 (pt) 2019-10-11 2022-07-12 Quantum Si Inc Modificação de superfície na fase de vapor
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US20210318238A1 (en) 2020-04-08 2021-10-14 Quantum-Si Incorporated Integrated sensor with reduced skew
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WO2022263644A1 (en) * 2021-06-17 2022-12-22 Roche Diagnostics Gmbh Thermostable affinity polypeptides
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Also Published As

Publication number Publication date
AU2021282471B2 (en) 2024-01-04
BR112017024573B1 (pt) 2024-03-05
HK1252483A1 (zh) 2019-05-24
AU2021282471A1 (en) 2022-01-06
EP3298389B1 (en) 2021-09-29
MX2017014821A (es) 2018-06-27
AU2016264753B2 (en) 2021-09-09
JP2018521308A (ja) 2018-08-02
CN108027325A (zh) 2018-05-11
TWI704229B (zh) 2020-09-11
JP6936736B2 (ja) 2021-09-22
CA2986237A1 (en) 2016-11-24
TW201708546A (zh) 2017-03-01
MX2022001187A (es) 2023-01-04
JP7339302B2 (ja) 2023-09-05
KR20180008779A (ko) 2018-01-24
AU2016264753A1 (en) 2017-12-14
WO2016187580A1 (en) 2016-11-24
EP3298389A1 (en) 2018-03-28
JP2021192044A (ja) 2021-12-16
EP3998471A1 (en) 2022-05-18

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