BR112018074572A2 - chamador de pulsos e chamador de base - Google Patents

chamador de pulsos e chamador de base

Info

Publication number
BR112018074572A2
BR112018074572A2 BR112018074572-3A BR112018074572A BR112018074572A2 BR 112018074572 A2 BR112018074572 A2 BR 112018074572A2 BR 112018074572 A BR112018074572 A BR 112018074572A BR 112018074572 A2 BR112018074572 A2 BR 112018074572A2
Authority
BR
Brazil
Prior art keywords
characteristic
nucleotide incorporation
caller
nucleotide
temporal
Prior art date
Application number
BR112018074572-3A
Other languages
English (en)
Other versions
BR112018074572B1 (pt
Inventor
M. Rothberg Jonathan
wenger Craig
Davey Mel
G. Fife Keith
JIA Jimmy
Reed Brian
J. GYARFAS Brett
Original Assignee
Quantum-Si Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum-Si Incorporated filed Critical Quantum-Si Incorporated
Publication of BR112018074572A2 publication Critical patent/BR112018074572A2/pt
Publication of BR112018074572B1 publication Critical patent/BR112018074572B1/pt

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G01N21/6454—Individual samples arranged in a regular 2D-array, e.g. multiwell plates using an integrated detector array
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Optics & Photonics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Data Mining & Analysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Burglar Alarm Systems (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

sistema e métodos para identificar nucleotídeos com base em dados adquiridos a partir de um sensor durante o sequenciamento de ácidos nucleicos. o método pode incluir obter características da luz detectada a partir de marcadores luminescentes associados aos nucleotídeos durante eventos de incorporação de nucleotídeos. as características podem incluir, para cada evento de incorporação de nucleotídeos, uma característica temporal da luz e uma característica de intensidade da luz. a característica temporal representa uma velocidade de declínio de uma probabilidade de emissão de fótons por um marcador luminescente após excitação. o método pode ainda incluir agrupar pontos que representam as características dos eventos de incorporação de nucleotídeos em grupos de pontos. os pontos individuais podem representar pelo menos a característica temporal e a característica de intensidade para um evento de incorporação de nucleotídeos correspondente. o método pode ainda incluir atribuir os grupos de pontos a nucleotídeos individuais.
BR112018074572-3A 2016-06-01 2017-06-01 Métodos de identificação de nucleotídeos e para calibrar um instrumento de sequenciamento, meio de armazenamento legível em computador não transitório, dispositivo e instrumento de sequenciamento BR112018074572B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662343997P 2016-06-01 2016-06-01
US62/343,997 2016-06-01
PCT/US2017/035420 WO2017210413A1 (en) 2016-06-01 2017-06-01 Pulse caller and base caller

Publications (2)

Publication Number Publication Date
BR112018074572A2 true BR112018074572A2 (pt) 2019-03-12
BR112018074572B1 BR112018074572B1 (pt) 2024-02-27

Family

ID=59055317

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018074572-3A BR112018074572B1 (pt) 2016-06-01 2017-06-01 Métodos de identificação de nucleotídeos e para calibrar um instrumento de sequenciamento, meio de armazenamento legível em computador não transitório, dispositivo e instrumento de sequenciamento

Country Status (11)

Country Link
US (2) US10711299B2 (pt)
EP (2) EP3465503B1 (pt)
JP (1) JP6931665B2 (pt)
KR (1) KR102425257B1 (pt)
CN (1) CN109313925B (pt)
AU (1) AU2017274412B2 (pt)
BR (1) BR112018074572B1 (pt)
CA (1) CA3026061A1 (pt)
MX (1) MX2018014856A (pt)
TW (1) TWI755400B (pt)
WO (1) WO2017210413A1 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9759658B2 (en) 2014-08-08 2017-09-12 Quantum-Si Incorporated Integrated device for temporal binning of received photons
JP2019512090A (ja) 2016-02-17 2019-05-09 テッセラクト ヘルス インコーポレイテッドＴｅｓｓｅｒａｃｔ Ｈｅａｌｔｈ，Ｉｎｃ． 寿命イメージングおよび検出用途のためのセンサおよびデバイス
CN114674788A (zh) * 2016-06-01 2022-06-28 宽腾矽公司 用于检测及分析分子的集成装置
MX2019007247A (es) 2016-12-19 2019-08-16 Quantum Si Inc Carga de moleculas en pocillos para muestra para su analisis.
AU2017382316B2 (en) 2016-12-22 2023-02-09 Quantum-Si Incorporated Integrated photodetector with direct binning pixel
US11120104B2 (en) 2017-03-01 2021-09-14 Stmicroelectronics (Research & Development) Limited Method and apparatus for processing a histogram output from a detector sensor
EP3619323A4 (en) 2017-05-05 2021-01-27 Quantum-si Incorporated SUBSTRATES WITH MODIFIED SURFACE REACTIVITY AND ANTIFOULING PROPERTIES IN BIOLOGICAL REACTIONS
CA3065780A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
CA3088687A1 (en) * 2018-01-26 2019-08-01 Quantum-Si Incorporated Machine learning enabled pulse and base calling for sequencing devices
BR112020024790A2 (pt) 2018-06-22 2021-03-02 Quantum-Si Incorporated fotodetector integrado com região de armazenamento de carga de tempo de detecção variado
CN112930476A (zh) * 2018-08-29 2021-06-08 宽腾矽公司 使用光子计数光电检测器以检测寿命的系统及方法
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
AU2020364058A1 (en) 2019-10-11 2022-05-26 Quantum-Si Incorporated Surface modification in the vapor phase
US11869917B2 (en) 2020-01-14 2024-01-09 Quantum-Si Incorporated Integrated sensor for lifetime characterization
JP2023510578A (ja) 2020-01-14 2023-03-14 クアンタム－エスアイ インコーポレイテッド 寿命およびスペクトル特性評価用センサ
EP4107735A2 (en) 2020-02-20 2022-12-28 Illumina, Inc. Artificial intelligence-based many-to-many base calling
JP2023515682A (ja) * 2020-03-02 2023-04-13 クアンタム－エスアイ インコーポレイテッド 多次元信号解析用集積センサ
CN115769377A (zh) 2020-04-08 2023-03-07 宽腾矽公司 偏斜减小的集成传感器
US20220128566A1 (en) * 2020-10-27 2022-04-28 Quantum-Si Incorporated Calibration of single-molecule detection system
US20220336054A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0682671A4 (en) * 1993-02-01 1998-01-14 Seq Ltd METHOD AND DEVICES FOR SEQUENCING DNA.
DE19744490A1 (de) * 1997-10-08 1999-04-15 Temp Rite International Gmbh Trinkwasseraufbereitungsanlage
JP4566299B2 (ja) * 1998-04-09 2010-10-20 シスメックス株式会社 赤芽球の分類計数方法
EP1104491A4 (en) * 1998-08-11 2003-01-29 Caliper Techn Corp DNA SEQUEN AGE BY DIFFERENTIATION OF THE PERIODS OF DECREASE IN EMISSION OF FLUORESCENT PROBES AND SYSTEMS THEREFOR
DE19844931C1 (de) * 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
GB2382648B (en) * 2001-12-11 2003-11-12 Amersham Pharm Biotech Uk Ltd System and method for time correlated multi-photon counting measurements
WO2005120204A2 (en) * 2004-06-07 2005-12-22 The Regents Of The University Of California Method for single molecule fluorescence analysis
CA2689626C (en) * 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2009085473A2 (en) 2007-11-21 2009-07-09 Cosmosid Inc. Genome identification system
US20110165652A1 (en) * 2008-01-14 2011-07-07 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
WO2011006165A2 (en) * 2009-07-10 2011-01-13 Perkinelmer Health Sciences, Inc. Detecting multinucleotide repeats
US9670243B2 (en) * 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
WO2012177792A2 (en) * 2011-06-24 2012-12-27 Sequenom, Inc. Methods and processes for non-invasive assessment of a genetic variation
US9145623B2 (en) * 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
JP5790242B2 (ja) * 2011-07-25 2015-10-07 富士ゼロックス株式会社 故障予測装置及びプログラム
WO2013148400A1 (en) * 2012-03-30 2013-10-03 Pacific Biosciences Of California, Inc. Methods and composition for sequencing modified nucleic acids
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
AU2014268420A1 (en) * 2013-05-23 2015-11-26 Iphenotype Llc Method and system for maintaining or improving wellness
AU2014348306B2 (en) 2013-11-17 2020-11-12 Quantum-Si Incorporated Active-source-pixel, integrated device for rapid analysis of biological and chemical specimens
EP3084002A4 (en) 2013-12-16 2017-08-23 Complete Genomics, Inc. Basecaller for dna sequencing using machine learning
CN106462670B (zh) 2014-05-12 2020-04-10 豪夫迈·罗氏有限公司 超深度测序中的罕见变体召集
CN106796175B (zh) 2014-08-08 2021-01-05 宽腾矽公司 用于探测、检测和分析分子的光学系统和检测芯片
US9759658B2 (en) * 2014-08-08 2017-09-12 Quantum-Si Incorporated Integrated device for temporal binning of received photons
EP3194934B1 (en) 2014-08-08 2024-03-06 Quantum-Si Incorporated Integrated device for use with an external light source for probing, detecting, and analyzing molecules by luminescence lifetime measurements
US10185803B2 (en) 2015-06-15 2019-01-22 Deep Genomics Incorporated Systems and methods for classifying, prioritizing and interpreting genetic variants and therapies using a deep neural network
US9922285B1 (en) 2017-07-13 2018-03-20 HumanCode, Inc. Predictive assignments that relate to genetic information and leverage machine learning models

Also Published As

Publication number Publication date
WO2017210413A1 (en) 2017-12-07
CN109313925A (zh) 2019-02-05
MX2018014856A (es) 2019-03-07
AU2017274412B2 (en) 2022-07-21
AU2017274412A1 (en) 2018-12-06
US10711299B2 (en) 2020-07-14
KR20190015371A (ko) 2019-02-13
JP2019529865A (ja) 2019-10-17
TWI755400B (zh) 2022-02-21
CA3026061A1 (en) 2017-12-07
EP4009330A1 (en) 2022-06-08
BR112018074572B1 (pt) 2024-02-27
CN109313925B (zh) 2022-09-02
US20170349944A1 (en) 2017-12-07
US20200291467A1 (en) 2020-09-17
EP3465503B1 (en) 2021-09-29
KR102425257B1 (ko) 2022-07-27
TW201800739A (zh) 2018-01-01
JP6931665B2 (ja) 2021-09-08
EP3465503A1 (en) 2019-04-10

Similar Documents

Publication Publication Date Title
BR112018074572A2 (pt) chamador de pulsos e chamador de base
AR102518A1 (es) Métodos y biomarcadores para predecir la eficacia y evaluación de un tratamiento con agonista de ox40
RU2017101353A (ru) Набор или устройство для обнаружения рака пищевода и способ обнаружения
CY1122632T1 (el) Μεθοδοι για μελετη πυρηνικων οξεων
CY1122994T1 (el) Μεθοδος καθαρισμου κανναβινοειλων, συνθεσεων και kit αυτων
RU2017101174A (ru) Набор или устройство для обнаружения рака печени и способ обнаружения
MX2019008347A (es) Secuenciamiento de canal bioptico, de una sola fuente de luz.
MX2020007904A (es) Llamado de pulso y base habilitado por maquina de aprendizaje para dispositivos de secuenciacion.
JP2019529865A5 (pt)
EA201692535A1 (ru) Замещенные нуклеозиды, нуклеотиды и их аналоги
RU2017100015A (ru) Набор или устройство для обнаружения рака желчных путей и способ обнаружения
ECSP16012693A (es) Polipéptido para la escisión hidrolítica de la zearalenona y/o derivados de la zearalenona, polinucleótido aislado de la misma y polipéptido que contiene aditivo, uso y procedimiento de los mismos
EP4321627A3 (en) Spatially distinguished, multiplex nucleic acid analysis of biological specimens
RU2017101023A (ru) Набор или устройство для обнаружения рака желудка и способ обнаружения
BR112016025751A2 (pt) roedor, e, métodos para fabricar um roedor e um camundongo humanizados e para identificar um composto capaz de modular a ativação de complemento
RU2016152143A (ru) Набор или устройство для обнаружения рака поджелудочной железы и способ обнаружения
CY1121037T1 (el) Αντινοηματικα πολυνουκλεοτιδια για να διεγειρεται παραλειψη εξονιου και μεθοδοι θεραπειας δυστροφιων
BR112021022865A2 (pt) Matriz e método para detectar informações espaciais de ácidos nucleicos
BR112018073378A2 (pt) composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos
MX2021011433A (es) Sistema.
BR112014021993B8 (pt) "métodos para detecção de uma variação de nucleotídeo em uma sequência de ácido nucleico alvo através de um ensaio de ptoce"
AR115018A1 (es) Método para aumentar el nivel de expresión de una molécula de ácido nucleico de interés en una célula
Lindh et al. Molecular epidemiology of H9N2 influenza viruses in Northern Europe
BR112017022724A2 (pt) planta de milho tolerante a herbicida dbn9858 e sequência de nucleotídeos e método para detectar a mesma
AR104805A1 (es) Métodos y composiciones para evaluar la capacidad de respuesta de los cánceres a los inhibidores de bet

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: A CLASSIFICACAO ANTERIOR ERA: G06F 19/22

Ipc: C12Q 1/68 (2018.01), G01N 21/64 (2006.01), G16B 30

B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/06/2017, OBSERVADAS AS CONDICOES LEGAIS