AR115018A1 - Método para aumentar el nivel de expresión de una molécula de ácido nucleico de interés en una célula - Google Patents

Método para aumentar el nivel de expresión de una molécula de ácido nucleico de interés en una célula

Info

Publication number
AR115018A1
AR115018A1 ARP190100757A ARP190100757A AR115018A1 AR 115018 A1 AR115018 A1 AR 115018A1 AR P190100757 A ARP190100757 A AR P190100757A AR P190100757 A ARP190100757 A AR P190100757A AR 115018 A1 AR115018 A1 AR 115018A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
interest
expression
cell
acid molecule
Prior art date
Application number
ARP190100757A
Other languages
English (en)
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of AR115018A1 publication Critical patent/AR115018A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente proporciona un método para aumentar el nivel de expresión de una molécula de ácido nucleico de interés en una célula, preferentemente, una célula vegetal, por medio de secuencias de ácido nucleico activadoras de promotor, que son capaces de aumentar la expresión de una molécula de ácido nucleico de interés en una célula o en un organismo luego de la introducción específica de sitio en un promotor receptor que controla la expresión de la molécula de ácido nucleico de interés. También proporciona métodos para identificar dichos elementos activadores de promotor y métodos para introducirlos en un organismo o en una célula para aumentar específicamente la expresión de una molécula de ácido nucleico de interés. Además, se refiere al uso de los elementos activadores de promotor para aumentar la expresión de una molécula de ácido nucleico de interés.
ARP190100757A 2018-03-26 2019-03-26 Método para aumentar el nivel de expresión de una molécula de ácido nucleico de interés en una célula AR115018A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18164080.6A EP3546582A1 (en) 2018-03-26 2018-03-26 Promoter activating elements

Publications (1)

Publication Number Publication Date
AR115018A1 true AR115018A1 (es) 2020-11-18

Family

ID=61868173

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190100757A AR115018A1 (es) 2018-03-26 2019-03-26 Método para aumentar el nivel de expresión de una molécula de ácido nucleico de interés en una célula

Country Status (7)

Country Link
US (1) US20210155948A1 (es)
EP (2) EP3546582A1 (es)
CN (1) CN111902541A (es)
AR (1) AR115018A1 (es)
BR (1) BR112020017535A2 (es)
CA (1) CA3095085A1 (es)
WO (1) WO2019185609A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3835309A1 (en) 2019-12-13 2021-06-16 KWS SAAT SE & Co. KGaA Method for increasing cold or frost tolerance in a plant
EP4118219A4 (en) * 2020-03-11 2024-04-17 Shanghai Belief Delivery Biomed Co Ltd NOVEL USE OF AN ASPIRIN COMPOUND TO INCREASE NUCLEIC ACID EXPRESSION
US20230242928A1 (en) * 2020-03-13 2023-08-03 Pioneer Hi-Bred International, Inc. Modulating nucleotide expression using expression modulating elements and modified tata and use thereof
US20230416692A1 (en) * 2020-03-27 2023-12-28 Board Of Regents, The University Of Texas System Reverse genetic system for sars-cov-2
EP4043574A1 (en) 2021-02-11 2022-08-17 KWS SAAT SE & Co. KGaA Synergistic promoter activation by combining cpe and cre modifications
EP4209502A1 (en) 2022-01-07 2023-07-12 KWS SAAT SE & Co. KGaA Fructose sensing and signaling in plants

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6072050A (en) * 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
EP0934406B1 (en) * 1996-08-23 2008-11-19 Peter Ruhdal Jensen Artificial promoter libraries for selected organisms and promoters derived from such libraries
TR200200693T2 (tr) * 1999-09-16 2002-07-22 Monsanto Technology Llc Gen ifadesinin kontrolü için bitkisel düzenleyici sekanslar
CN105219773B (zh) * 2013-12-25 2017-09-26 北京大北农科技集团股份有限公司 嵌合启动子及其用途
EP3178935B1 (en) 2014-08-06 2022-12-21 Toolgen Incorporated Genome editing using campylobacter jejuni crispr/cas system-derived rgen
ES2743512T3 (es) * 2015-03-06 2020-02-19 Leibniz Inst Fuer Pflanzenbiochemie Ipb Biblioteca de promotores sintéticos para la expresión génica coordinada en células u organismos eucariotas
EP3095870A1 (en) * 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems

Also Published As

Publication number Publication date
CA3095085A1 (en) 2019-10-03
US20210155948A1 (en) 2021-05-27
EP3546582A1 (en) 2019-10-02
BR112020017535A2 (pt) 2020-12-22
WO2019185609A1 (en) 2019-10-03
CN111902541A (zh) 2020-11-06
EP3775223A1 (en) 2021-02-17

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