BR112022008003A2 - Métodos, kits e dispositivos de preparação de amostras para sequenciamento polipeptídico multiplex - Google Patents

Métodos, kits e dispositivos de preparação de amostras para sequenciamento polipeptídico multiplex

Info

Publication number
BR112022008003A2
BR112022008003A2 BR112022008003A BR112022008003A BR112022008003A2 BR 112022008003 A2 BR112022008003 A2 BR 112022008003A2 BR 112022008003 A BR112022008003 A BR 112022008003A BR 112022008003 A BR112022008003 A BR 112022008003A BR 112022008003 A2 BR112022008003 A2 BR 112022008003A2
Authority
BR
Brazil
Prior art keywords
sample preparation
kits
devices
polypeptide sequencing
preparation methods
Prior art date
Application number
BR112022008003A
Other languages
English (en)
Inventor
Dyer Matthew
Reed Brian
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of BR112022008003A2 publication Critical patent/BR112022008003A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/023—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution using racemisation inhibiting agents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/045—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers using devices to improve synthesis, e.g. reactors, special vessels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

MÉTODOS, KITS E DISPOSITIVOS DE PREPARAÇÃO DE AMOSTRAS PARA SEQUENCIAMENTO POLIPEPTÍDICO MULTIPLEX. A presente invenção refere-se a métodos de preparação de uma amostra multiplexada para sequenciamento polipeptídico usando códigos de barras. Métodos para amostras multiplexadas para sequenciamento polipeptídico no qual as populações polipeptídicas são fisicamente separadas. Kit que compreende uma população de códigos de barras e dispositivo para preparar uma amostra que compreende um módulo de preparação de amostra que compreende códigos de barras e sondas de captura imobilizadas configuradas para interagir com um cartucho que compreende um reservatório.
BR112022008003A 2019-10-28 2020-10-28 Métodos, kits e dispositivos de preparação de amostras para sequenciamento polipeptídico multiplex BR112022008003A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962926975P 2019-10-28 2019-10-28
PCT/US2020/057647 WO2021086908A1 (en) 2019-10-28 2020-10-28 Methods, kits and devices of preparing samples for multiplex polypeptide sequencing

Publications (1)

Publication Number Publication Date
BR112022008003A2 true BR112022008003A2 (pt) 2022-07-12

Family

ID=73646413

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022008003A BR112022008003A2 (pt) 2019-10-28 2020-10-28 Métodos, kits e dispositivos de preparação de amostras para sequenciamento polipeptídico multiplex

Country Status (10)

Country Link
US (1) US20210147474A1 (pt)
EP (1) EP4041911A1 (pt)
JP (1) JP2023500486A (pt)
KR (1) KR20220108054A (pt)
CN (1) CN114929888A (pt)
AU (1) AU2020376809A1 (pt)
BR (1) BR112022008003A2 (pt)
CA (1) CA3159402A1 (pt)
MX (1) MX2022005092A (pt)
WO (1) WO2021086908A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113287020A (zh) 2018-11-15 2021-08-20 宽腾矽公司 用于蛋白质测序的方法及组合物
WO2023245129A2 (en) * 2022-06-15 2023-12-21 Quantum-Si Incorporated Directed protein evolution

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
WO2010065531A1 (en) * 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
WO2010065322A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
WO2013112745A1 (en) * 2012-01-24 2013-08-01 The Regents Of The University Of Colorado, A Body Corporate Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
CA2961493C (en) * 2014-09-15 2023-10-03 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
US11634706B2 (en) * 2014-09-18 2023-04-25 The Trustees of the California State University Single-molecule phenotype analysis
MX2018013341A (es) * 2016-05-02 2019-09-18 Encodia Inc Analisis de macromoleculas que emplea la codificacion de acido nucleico.
US10208347B2 (en) * 2016-05-25 2019-02-19 Bioinventors & Entrepreneurs Network, Llc Attribute sieving and profiling with sample enrichment by optimized pooling
US11072816B2 (en) * 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
CA3081446A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
EP3775261A4 (en) * 2018-03-26 2021-11-24 Rootpath Genomics, Inc. COMPOSITIONS OF TARGET UNITS AND METHODS OF USE
WO2020154307A1 (en) * 2019-01-22 2020-07-30 Singular Genomics Systems, Inc. Polynucleotide barcodes for multiplexed proteomics
WO2020219365A1 (en) * 2019-04-23 2020-10-29 Encodia, Inc. Methods for spatial analysis of proteins and related kits

Also Published As

Publication number Publication date
JP2023500486A (ja) 2023-01-06
US20210147474A1 (en) 2021-05-20
EP4041911A1 (en) 2022-08-17
CN114929888A (zh) 2022-08-19
AU2020376809A1 (en) 2022-06-02
WO2021086908A1 (en) 2021-05-06
MX2022005092A (es) 2022-08-15
CA3159402A1 (en) 2021-05-06
KR20220108054A (ko) 2022-08-02

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]