BR112017019774A2 - uso de uma membrana capilar porosa para determinar a quantidade de produtos de am-plificação por círculo rolante - Google Patents

uso de uma membrana capilar porosa para determinar a quantidade de produtos de am-plificação por círculo rolante

Info

Publication number
BR112017019774A2
BR112017019774A2 BR112017019774A BR112017019774A BR112017019774A2 BR 112017019774 A2 BR112017019774 A2 BR 112017019774A2 BR 112017019774 A BR112017019774 A BR 112017019774A BR 112017019774 A BR112017019774 A BR 112017019774A BR 112017019774 A2 BR112017019774 A2 BR 112017019774A2
Authority
BR
Brazil
Prior art keywords
rca
amount
rolling circle
labeled
amplification products
Prior art date
Application number
BR112017019774A
Other languages
English (en)
Other versions
BR112017019774B1 (pt
Inventor
Persson Frederik
HOWELL Mathias
Öhman Ove
Original Assignee
Vanadis Diagnostics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vanadis Diagnostics filed Critical Vanadis Diagnostics
Publication of BR112017019774A2 publication Critical patent/BR112017019774A2/pt
Publication of BR112017019774B1 publication Critical patent/BR112017019774B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

a presente invenção refere-se a um método de análise de amostra. em certas modalidades, o método pode compreender: (a) filtrar uma amostra líquida contendo produtos de amplificação por círculo rolante (rca) usando uma membrana capilar porosa, produzindo assim uma matriz de produtos de rca na membrana; em que a amostra contém pelo menos uma primeira população de produtos de rca e uma segunda população de produtos de rca, em que as primeira e segunda populações de produtos de rca marcados são marcadas de forma distinta; e (b) determinar a quantidade da primeira população marcada de produtos de rca e a quantidade da segunda população marcada de produtos de rca em uma área da membrana.
BR112017019774-0A 2015-04-30 2016-05-02 Método de análise de amostra através do uso de uma membrana capilar porosa para determinar a quantidade de produtos de amplificação por círculo rolante BR112017019774B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1507376.0 2015-04-30
GBGB1507376.0A GB201507376D0 (en) 2015-04-30 2015-04-30 Use of a porous transparent capillary membrane for counting rolling circle amplification products
PCT/IB2016/052495 WO2016174649A1 (en) 2015-04-30 2016-05-02 Use of a porous capillary membrane for determining the amount of rolling circle amplification products

Publications (2)

Publication Number Publication Date
BR112017019774A2 true BR112017019774A2 (pt) 2018-05-15
BR112017019774B1 BR112017019774B1 (pt) 2024-05-14

Family

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Also Published As

Publication number Publication date
CA2978524C (en) 2024-05-28
IL254343B (en) 2021-09-30
ES2803048T3 (es) 2021-01-22
JP6874252B2 (ja) 2021-05-19
IL254343A0 (en) 2017-11-30
RU2017133063A (ru) 2019-03-22
US20190211383A1 (en) 2019-07-11
KR20170140177A (ko) 2017-12-20
AU2016255964B2 (en) 2021-09-30
JP2018515067A (ja) 2018-06-14
US10934579B2 (en) 2021-03-02
DK3289099T3 (da) 2020-07-20
CA2978524A1 (en) 2016-11-03
EP3289099B1 (en) 2020-04-15
RU2017133063A3 (pt) 2019-11-15
US10208336B2 (en) 2019-02-19
EP3289099A1 (en) 2018-03-07
GB201507376D0 (en) 2015-06-17
CN106460073A (zh) 2017-02-22
PL3289099T3 (pl) 2020-09-07
CN106460073B (zh) 2020-03-31
KR102587191B1 (ko) 2023-10-06
WO2016174649A1 (en) 2016-11-03
AU2016255964A1 (en) 2017-09-21
RU2725196C2 (ru) 2020-06-30
US20170022548A1 (en) 2017-01-26

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 02/05/2016, OBSERVADAS AS CONDICOES LEGAIS