BR112018068303A2 - método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida - Google Patents
método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluidaInfo
- Publication number
- BR112018068303A2 BR112018068303A2 BR112018068303A BR112018068303A BR112018068303A2 BR 112018068303 A2 BR112018068303 A2 BR 112018068303A2 BR 112018068303 A BR112018068303 A BR 112018068303A BR 112018068303 A BR112018068303 A BR 112018068303A BR 112018068303 A2 BR112018068303 A2 BR 112018068303A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell culture
- culture system
- cells
- microfluid cell
- microfluid
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/089—Virtual walls for guiding liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/163—Biocompatibility
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1392—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from mesenchymal stem cells from other natural sources
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/23—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the gastro-intestinal tract
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2531/00—Microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
a presente invenção se refere a um método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida caracterizado por compreender uma rede microfluida de canais. no método, células epiteliais são alinhadas no sistema de cultura microfluido de células por células de origem mesenquimal. as células podem formar uma estrutura tubular ou similar a tubular, por exemplo um tubo em um tubo. o método possibilita modelos epiteliais aprimorados adequados para uma ampla variedade de aplicações, incluindo sem limitações triagem de alto rendimento e análise de epitélio em saúde e doença.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NL2016404A NL2016404B1 (en) | 2016-03-09 | 2016-03-09 | Double tubular structures. |
PCT/NL2017/050145 WO2017155399A1 (en) | 2016-03-09 | 2017-03-09 | Double tubular structures |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112018068303A2 true BR112018068303A2 (pt) | 2019-03-06 |
Family
ID=55967375
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018068303A BR112018068303A2 (pt) | 2016-03-09 | 2017-03-09 | método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida |
Country Status (11)
Country | Link |
---|---|
US (1) | US20190076842A1 (pt) |
EP (1) | EP3426769A1 (pt) |
JP (1) | JP7117245B2 (pt) |
KR (1) | KR102403435B1 (pt) |
CN (1) | CN109072184A (pt) |
AU (1) | AU2017231580B2 (pt) |
BR (1) | BR112018068303A2 (pt) |
CA (1) | CA3017100A1 (pt) |
NL (1) | NL2016404B1 (pt) |
RU (1) | RU2756404C1 (pt) |
WO (1) | WO2017155399A1 (pt) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL2020518B1 (en) | 2018-03-02 | 2019-09-12 | Mimetas B V | Device and method for performing electrical measurements |
WO2020073043A1 (en) | 2018-10-05 | 2020-04-09 | The Trustees Of The University Of Pennsylvania | Artificial human pulmonary airway and methods of preparation |
CN109926112A (zh) * | 2019-04-10 | 2019-06-25 | 中南大学 | 三维微结构聚合物微流控芯片的叠层制造方法 |
NL2024202B1 (en) * | 2019-11-08 | 2021-07-20 | Mimetas B V | Microfluidic cell culture system |
WO2022225602A1 (en) * | 2021-04-23 | 2022-10-27 | Massachusetts Institute Of Technology | Fluidic platforms for perfusable vascularized tissues with infiltrates |
WO2024072512A1 (en) * | 2022-09-30 | 2024-04-04 | Xellar, Inc. | Methods and systems for functionalizing surfaces for microfluidic devices or other applications |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4829000A (en) | 1985-08-30 | 1989-05-09 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Reconstituted basement membrane complex with biological activity |
US9157060B2 (en) * | 2005-06-09 | 2015-10-13 | The United States Of America, As Represented By The Secretary Of The Navy | Micro blood vessels and tissue ducts |
US8287854B2 (en) * | 2005-10-21 | 2012-10-16 | Cellresearch Corporation Pte Ltd | Skin equivalents derived from umbilical cord mesenchymal stem/progenitor cells and umbilical cord epithelial stem/progenitor cells |
US20110129850A1 (en) | 2006-12-22 | 2011-06-02 | UCLA Office of Intellectual Property | Microfluidic platform for cell culture and assay |
US20110033430A1 (en) * | 2007-02-09 | 2011-02-10 | The General Hospital Corporation | Methods for the induction of a cell to enter the islet 1+ lineage and a method for the expansion thereof |
US9303245B2 (en) * | 2008-06-20 | 2016-04-05 | Universiteit Maastricht | Self-assembling tissue modules |
EP2213364A1 (en) | 2009-01-30 | 2010-08-04 | Albert-Ludwigs-Universität Freiburg | Phase guide patterns for liquid manipulation |
US9752124B2 (en) | 2009-02-03 | 2017-09-05 | Koninklijke Nederlandse Akademie Van Wetenschappen | Culture medium for epithelial stem cells and organoids comprising the stem cells |
TW201137121A (en) * | 2010-04-26 | 2011-11-01 | Univ Nat Changhua Education | Cell culture real-time observation system |
KR102351944B1 (ko) * | 2011-02-28 | 2022-01-18 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 세포 배양 시스템 |
GB201103917D0 (en) | 2011-03-08 | 2011-04-20 | Univ Leiden | Apparatus for and methods of processing liquids or liquid based substances |
CN103087912A (zh) * | 2011-10-27 | 2013-05-08 | 中国科学院大连化学物理研究所 | 一种产生稳定浓度梯度的微流控芯片及细胞共培养方法 |
US20140335496A1 (en) * | 2011-12-05 | 2014-11-13 | Research Triangle Institute | Human conducting airway model comprising multiple fluidic pathways |
EP2834649A4 (en) | 2012-04-01 | 2015-12-09 | Emd Millipore Corp | CELL CULTURE AND GRADIENT MIGRATION TEST METHOD AND DEVICES |
GB2505706A (en) | 2012-09-10 | 2014-03-12 | Univ Leiden | Apparatus comprising meniscus alignment barriers |
SG11201609066QA (en) * | 2014-05-05 | 2016-11-29 | Univ Singapore | Methods of producing tissue-mimetic constructs and uses thereof |
US10221382B2 (en) * | 2014-05-20 | 2019-03-05 | The University Of Tokyo | Hollow microfiber |
-
2016
- 2016-03-09 NL NL2016404A patent/NL2016404B1/en active
-
2017
- 2017-03-09 EP EP17715816.9A patent/EP3426769A1/en active Pending
- 2017-03-09 KR KR1020187029134A patent/KR102403435B1/ko active IP Right Grant
- 2017-03-09 AU AU2017231580A patent/AU2017231580B2/en active Active
- 2017-03-09 CN CN201780028395.8A patent/CN109072184A/zh active Pending
- 2017-03-09 WO PCT/NL2017/050145 patent/WO2017155399A1/en active Application Filing
- 2017-03-09 CA CA3017100A patent/CA3017100A1/en active Pending
- 2017-03-09 BR BR112018068303A patent/BR112018068303A2/pt not_active Application Discontinuation
- 2017-03-09 RU RU2018132008A patent/RU2756404C1/ru active
- 2017-03-09 US US16/083,372 patent/US20190076842A1/en active Pending
- 2017-03-09 JP JP2018547340A patent/JP7117245B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
CA3017100A1 (en) | 2017-09-14 |
RU2756404C1 (ru) | 2021-09-30 |
KR102403435B1 (ko) | 2022-05-30 |
JP7117245B2 (ja) | 2022-08-12 |
AU2017231580B2 (en) | 2023-03-30 |
KR20180131569A (ko) | 2018-12-10 |
NL2016404B1 (en) | 2017-09-26 |
WO2017155399A1 (en) | 2017-09-14 |
NL2016404A (en) | 2017-09-19 |
JP2019507602A (ja) | 2019-03-22 |
US20190076842A1 (en) | 2019-03-14 |
AU2017231580A1 (en) | 2018-09-27 |
EP3426769A1 (en) | 2019-01-16 |
CN109072184A (zh) | 2018-12-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112018068303A2 (pt) | método de cultivo e/ou monitoramento de células epiteliais usando um sistema de cultura celular microfluida, uso das células no sistema de cultura celular microfluida, composição ou sistema e sistema de cultura celular microfluida | |
BR112016023304A2 (pt) | célula hospedeira recombinante engendrada para superexpressar proteínas auxiliares | |
CU20180024A7 (es) | Moléculas de unión a linfopoyetina estromal tímica (tslp), composiciones farmacéuticas que las contienen y método de producción de las mismas | |
BR112016023769A2 (pt) | célula hospedeira recombinante para a expressão de proteínas de interesse | |
BR112018002214A2 (pt) | ?métodos para cultivar células e para produzir uma proteína, meios de cultura de células cho e de batelada alimentada e/ou alimentação, e, alimentação de cultura de células cho? | |
BR112019000430A2 (pt) | sistema crispr-cas para uma célula hospedeira de algas | |
BR112017017025A2 (pt) | métodos e composições para analizar componentes celulares | |
BR112015022490A2 (pt) | métodos e composições para classificação de amostras | |
BR112012019023A2 (pt) | método para produzir adenovírus recombinante sorotipo 26 (rad26), e, usoi de um biorreator | |
AR090017A1 (es) | Agonistas de fgfr1 y sus metodos de uso | |
BR112018004965A2 (pt) | domínios de superfamìlia de imunoglobulina variante ajustàvel | |
BR112016020287A2 (pt) | células resistentes a vírus e usos das mesmas | |
BR112015017307A2 (pt) | composições de espécies com baixa acidez e métodos para produção e uso das mesmas | |
UY32501A (es) | Anticuerpo antagonista específico para el heterodímero alfa4-beta7 | |
AR095348A1 (es) | Medios de cultivo celular y métodos de producción de anticuerpos | |
DK3284816T3 (da) | Dyrkningsmedietilsætningsstof, dyrkningsmediesammensætning og fremgangsmåde til at dyrke celler eller væv ved anvendelse af samme | |
WO2018053365A8 (en) | METHODS OF PREPARING NUCLEIC ACID SAMPLE FOR ANALYSIS OF ACELLULAR DNA | |
EA201791275A1 (ru) | Способы получения ctp-модифицированных полипептидов длительного действия | |
BR112017019774A2 (pt) | uso de uma membrana capilar porosa para determinar a quantidade de produtos de am-plificação por círculo rolante | |
BR112018014118A2 (pt) | sistema e processo de medida de uma propriedade de escoamento de um fluido no seio de um meio poroso | |
BR112015021993A8 (pt) | polipeptídeo, métodos para sua produção, métodos para cultivo de uma célula, composição farmacêutica, kit, e meio de cultura celular | |
WO2016094583A3 (en) | Biomarkers of oocyte quality | |
WO2016154623A3 (en) | Anti-cd133 monoclonal antibodies and related compositions and methods | |
BR112016028512A8 (pt) | método de cultivo de uma célula de mamífero e método de produção de uma proteína recombinante | |
BR112017003167A2 (pt) | método in vitro e aparelho para análise do comportamento de substâncias em ambiente fisiológico simulado |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |