GB2490846A - Detection of nucleic acid lesions and adducts using nanopores - Google Patents

Detection of nucleic acid lesions and adducts using nanopores Download PDF

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Publication number
GB2490846A
GB2490846A GB1216010.7A GB201216010A GB2490846A GB 2490846 A GB2490846 A GB 2490846A GB 201216010 A GB201216010 A GB 201216010A GB 2490846 A GB2490846 A GB 2490846A
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GB
United Kingdom
Prior art keywords
nucleic acid
lesion
nanopores
adducts
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1216010.7A
Other versions
GB201216010D0 (en
Inventor
Cynthia J Burrows
Henry S White
Ryuji Kawano
Aaron M Fleming
Na An
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation UURF
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University of Utah Research Foundation UURF
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Application filed by University of Utah Research Foundation UURF filed Critical University of Utah Research Foundation UURF
Publication of GB201216010D0 publication Critical patent/GB201216010D0/en
Publication of GB2490846A publication Critical patent/GB2490846A/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods, systems, and compounds for detecting modified nucleic acid bases are disclosed and described. In one example, a method of detecting a nucleic acid lesion can include directing a nucleic acid adduct into a channel, the nucleic acid adduct including a nucleic acid having a lesion and a current modulating compound coupled to the nucleic acid at the lesion (110), and measuring changes in current through the channel in response to the current modulating compound to detect the lesion (112). In some cases the method can include forming the nucleic acid adduct.
GB1216010.7A 2010-03-05 2011-03-07 Detection of nucleic acid lesions and adducts using nanopores Withdrawn GB2490846A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31082210P 2010-03-05 2010-03-05
PCT/US2011/027433 WO2011109825A2 (en) 2010-03-05 2011-03-07 Detection of nucleic acid lesions and adducts using nanopores

Publications (2)

Publication Number Publication Date
GB201216010D0 GB201216010D0 (en) 2012-10-24
GB2490846A true GB2490846A (en) 2012-11-14

Family

ID=44542879

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1216010.7A Withdrawn GB2490846A (en) 2010-03-05 2011-03-07 Detection of nucleic acid lesions and adducts using nanopores

Country Status (2)

Country Link
GB (1) GB2490846A (en)
WO (1) WO2011109825A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2201136B1 (en) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Nanopore sequencing by hybridization of probes to form ternary complexes and variable range alignment
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
CN102186989B (en) 2008-09-03 2021-06-29 纳伯塞斯2.0有限责任公司 Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US9005425B2 (en) 2010-03-05 2015-04-14 University Of Utah Research Foundation Detection of nucleic acid lesions and adducts using nanopores
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
JP5998148B2 (en) 2010-11-16 2016-09-28 ナブシス ２．０ エルエルシー Method for sequencing biomolecules by detecting the relative position of hybridized probes
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
GB2498021A (en) * 2011-09-07 2013-07-03 Univ Utah Res Found Detection of Nucleic acid lesions and adducts using nanopores
US20140087390A1 (en) * 2012-09-26 2014-03-27 Nabsys, Inc. Method and system for analysis of protein and other modifications on dna and rna
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080311582A1 (en) * 2005-11-15 2008-12-18 Hagan Bayley Methods Using Pores

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080311582A1 (en) * 2005-11-15 2008-12-18 Hagan Bayley Methods Using Pores

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Analytical Biochemistry, Vol. 343(1), 2005, ZHOU, X. et al. 'Quantification of DNA strand breaks and abasic sites by oxime derivatization and accelerator mass spectometry....', Pages 84-92 *
Angewandte Chemie, Vol.47, 2008, MITCHELL, N. and HOWORKA, S. 'Chemical tags facilitate the sensing of individual DNA strands with nanopores', Pages 5565-5568 *
Journal of the American Chemical Society, Vol. 131(37), 2009, KOJIMA, N. et al. 'Construction of highly reactive trpbes for abasic site detection....', Pages 13208-13209 *
Journal of the Americsn Chemical Society, vol. 130, 2008, XU. X. et al. 'Formation of tricyclic [4.3.3.0] adducts between 8-oxoguanosine and tyrosine under conditions of...', Pages 10080-10081 *

Also Published As

Publication number Publication date
WO2011109825A2 (en) 2011-09-09
WO2011109825A3 (en) 2012-03-08
GB201216010D0 (en) 2012-10-24

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