MY144323A - Method for linking sequences of interest - Google Patents

Method for linking sequences of interest

Info

Publication number
MY144323A
MY144323A MYPI20043820A MYPI20043820A MY144323A MY 144323 A MY144323 A MY 144323A MY PI20043820 A MYPI20043820 A MY PI20043820A MY PI20043820 A MYPI20043820 A MY PI20043820A MY 144323 A MY144323 A MY 144323A
Authority
MY
Malaysia
Prior art keywords
sequences
extension
pcr
interest
multiplex overlap
Prior art date
Application number
MYPI20043820A
Inventor
Martin B Oleksiewicz
Lars S Nielsen
Peter S Andersen
Margit H Hansen
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Publication of MY144323A publication Critical patent/MY144323A/en

Links

Abstract

MULTIPLEX OVERLAP-EXTENSION RT-PCR PROVIDES AN EFFICIENT METHOD OF LINKING TWO OR MORE NUCLEOTIDE SEQUENCES ENCODING FOR DOMAINS OR SUBUNITS OF A HETEROMERIC PROTEIN, IN A SINGLE REACTION. ESPECIALLY, THE LINKAGE OF VARIABLE REGION ENCODING SEQUENCES FROM E.G. IMMUNOGLOBULINS , T CELL RECEPTOR IS EASED WITH THE METHOD OF THE PRESENT INVENTION. THIS ALLOW FOR A MORE EFFICIENT WAY OF GENERATING LIBRARIES OF VARIABLE REGIONENCODING SEQUENCES. THE CAPABILITY TO PERFORM THE MULTIPLEX OVERLAP-EXTENSION RT-PCR USING TEMPLATE DERIVED FROM AN ISOLATED SINGLE CELL ENABLES THE GENERATION OF COGNATE PAIR LIBRARIES IN A HIGH-THROUGHPUT FORMAT.
MYPI20043820A 2003-09-18 2004-09-17 Method for linking sequences of interest MY144323A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50458903P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15

Publications (1)

Publication Number Publication Date
MY144323A true MY144323A (en) 2011-08-29

Family

ID=40257283

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI20043820A MY144323A (en) 2003-09-18 2004-09-17 Method for linking sequences of interest

Country Status (3)

Country Link
AR (1) AR045756A1 (en)
MY (1) MY144323A (en)
PA (1) PA8612801A1 (en)

Also Published As

Publication number Publication date
AR045756A1 (en) 2005-11-09
PA8612801A1 (en) 2005-03-28

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