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Publication of MY144323ApublicationCriticalpatent/MY144323A/en
MULTIPLEX OVERLAP-EXTENSION RT-PCR PROVIDES AN EFFICIENT METHOD OF LINKING TWO OR MORE NUCLEOTIDE SEQUENCES ENCODING FOR DOMAINS OR SUBUNITS OF A HETEROMERIC PROTEIN, IN A SINGLE REACTION. ESPECIALLY, THE LINKAGE OF VARIABLE REGION ENCODING SEQUENCES FROM E.G. IMMUNOGLOBULINS , T CELL RECEPTOR IS EASED WITH THE METHOD OF THE PRESENT INVENTION. THIS ALLOW FOR A MORE EFFICIENT WAY OF GENERATING LIBRARIES OF VARIABLE REGIONENCODING SEQUENCES. THE CAPABILITY TO PERFORM THE MULTIPLEX OVERLAP-EXTENSION RT-PCR USING TEMPLATE DERIVED FROM AN ISOLATED SINGLE CELL ENABLES THE GENERATION OF COGNATE PAIR LIBRARIES IN A HIGH-THROUGHPUT FORMAT.
MYPI20043820A2003-09-182004-09-17Method for linking sequences of interest
MY144323A
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