NO20061373L - Fremgangsmate for a linke interessante sekvenser - Google Patents

Fremgangsmate for a linke interessante sekvenser

Info

Publication number
NO20061373L
NO20061373L NO20061373A NO20061373A NO20061373L NO 20061373 L NO20061373 L NO 20061373L NO 20061373 A NO20061373 A NO 20061373A NO 20061373 A NO20061373 A NO 20061373A NO 20061373 L NO20061373 L NO 20061373L
Authority
NO
Norway
Prior art keywords
sequences encoding
linking
procedure
variable regions
overlap extension
Prior art date
Application number
NO20061373A
Other languages
English (en)
Inventor
Martin B Oleksiewicz
Lars S Nielsen
Peter S Andersen
Margit H Hansen
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/DK2004/000633 external-priority patent/WO2005042774A2/en
Application filed by Symphogen As filed Critical Symphogen As
Publication of NO20061373L publication Critical patent/NO20061373L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Multipleks overlapp-forlengelses RT-PCR fremskaffer en effektiv fremgangsmåte for linking av to eller flere nukleotidsekvenser kodende for områder eller subenheter av et heteromert protein i en enkel reaksjon. Spesielt blir koplingen av sekvenser som koder for variable regioner fra f eks. immunoglobuliner. T-celle reseptorer eller B-celle reseptorer forenklet ved fremgangsmåten ifølge foreliggende oppfinnelse. Dette tillater en mer effektiv måte for generering av biblioteker av sekvenser som koder for variable regioner. Derme muligheten for å utføre den multiplekse overlapp- forlengelses RT-PCR ved bruk av templat avledet fra en isolert enkeltcelle muliggjør genereringen av bibliotek av kognate par i et høygjennomløpsformat.
NO20061373A 2003-09-18 2006-03-27 Fremgangsmate for a linke interessante sekvenser NO20061373L (no)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US50445503P 2003-09-18 2003-09-18
US50458903P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15
PCT/DK2004/000633 WO2005042774A2 (en) 2003-09-18 2004-09-17 Method for linking sequences of interest

Publications (1)

Publication Number Publication Date
NO20061373L true NO20061373L (no) 2006-03-27

Family

ID=34199056

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20061373A NO20061373L (no) 2003-09-18 2006-03-27 Fremgangsmate for a linke interessante sekvenser

Country Status (7)

Country Link
KR (1) KR101135134B1 (no)
CY (2) CY1108119T1 (no)
HK (2) HK1110893A1 (no)
HR (2) HRP20080197T8 (no)
IL (1) IL173688A (no)
IS (1) IS2583B (no)
NO (1) NO20061373L (no)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY201744A (en) * 2016-12-23 2024-03-15 Visterra Inc Binding polypeptides and methods of making the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0597960T3 (da) * 1991-08-10 1999-09-13 Medical Res Council Behandling af cellepopulationer

Also Published As

Publication number Publication date
IL173688A (en) 2010-12-30
HRP20080197T3 (en) 2008-05-31
CY1112657T1 (el) 2016-02-10
KR101135134B1 (ko) 2012-04-13
HK1085513A1 (en) 2006-08-25
CY1108119T1 (el) 2014-02-12
IS8415A (is) 2006-04-12
IS2583B (is) 2010-02-15
KR20060092218A (ko) 2006-08-22
HRP20080197T8 (en) 2008-07-31
HRP20120039T1 (hr) 2012-02-29
HK1110893A1 (en) 2008-07-25
IL173688A0 (en) 2006-07-05

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Legal Events

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FC2A Withdrawal, rejection or dismissal of laid open patent application