CY1108119T1 - Μεθοδος συνδεσης ενδιαφερουσων αλυσιδων - Google Patents

Μεθοδος συνδεσης ενδιαφερουσων αλυσιδων

Info

Publication number
CY1108119T1
CY1108119T1 CY20081100597T CY081100597T CY1108119T1 CY 1108119 T1 CY1108119 T1 CY 1108119T1 CY 20081100597 T CY20081100597 T CY 20081100597T CY 081100597 T CY081100597 T CY 081100597T CY 1108119 T1 CY1108119 T1 CY 1108119T1
Authority
CY
Cyprus
Prior art keywords
chains
extension
allows
libraries
interference
Prior art date
Application number
CY20081100597T
Other languages
English (en)
Inventor
Martin B Oleksiewicz
Lars S Nielsen
Peter S Andersen
Margit H Hansen
Original Assignee
Symphogen A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP04762853A external-priority patent/EP1670912B1/en
Application filed by Symphogen A/S filed Critical Symphogen A/S
Publication of CY1108119T1 publication Critical patent/CY1108119T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Η πολλαπλή RT-PCR υπερκάλυψης-επέκτασης παρέχει μία αποτελεσματική μέθοδο σύνδεσης δύο ή περισσότερων αλυσίδων νουκλεοτιδίων (nucleotide) που κωδικοποιούν περιοχές ή υπομονάδες μιας ετερομερούς πρωτεΐνης, σε μία απλή αντίδραση. Ειδικότερα η σύνδεση των αλυσίδων κωδικοποίησης από π.χ. ανοσογλοβουλίνες, λήπτες Τ κυττάρων ή λήπτες Β κυττάρων διευκολύνεται με την μέθοδο τητς παρούσας εφεύρεσης. Αυτό επιτρέπει πιο αποτελεσματικό τρόπο δημιουργίας βιβλιοθηκών αλυσίδων κωδικοποίησης μεταβλητής περιοχής. Η ικανότητα πραγματοποίησης πολλαπλής RT-PCR υπερκάλυψης-επέκτασης χρησιμοποιώντας μία φόρμα που παράγεται από ένα απομονωμένο ξεχωριστό κύτταρο επιτρέπει την δημιουργία βιβλιοθηκών συγγενών ζευγών σε μία μορφή υψηλής παραγωγής.
CY20081100597T 2003-09-18 2008-06-05 Μεθοδος συνδεσης ενδιαφερουσων αλυσιδων CY1108119T1 (el)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US50458903P 2003-09-18 2003-09-18
US50445503P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15
EP04762853A EP1670912B1 (en) 2003-09-18 2004-09-17 Method for linking sequences of interest

Publications (1)

Publication Number Publication Date
CY1108119T1 true CY1108119T1 (el) 2014-02-12

Family

ID=34199056

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20081100597T CY1108119T1 (el) 2003-09-18 2008-06-05 Μεθοδος συνδεσης ενδιαφερουσων αλυσιδων
CY20121100200T CY1112657T1 (el) 2003-09-18 2012-02-24 Μεθοδος συνδεσης ενδιαφερουσων αλυσιδων

Family Applications After (1)

Application Number Title Priority Date Filing Date
CY20121100200T CY1112657T1 (el) 2003-09-18 2012-02-24 Μεθοδος συνδεσης ενδιαφερουσων αλυσιδων

Country Status (7)

Country Link
KR (1) KR101135134B1 (el)
CY (2) CY1108119T1 (el)
HK (2) HK1085513A1 (el)
HR (2) HRP20080197T8 (el)
IL (1) IL173688A (el)
IS (1) IS2583B (el)
NO (1) NO20061373L (el)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2114950A1 (en) * 1991-08-10 1993-02-11 Michael J. Embleton Treatment of cell populations

Also Published As

Publication number Publication date
HRP20080197T8 (en) 2008-07-31
HK1110893A1 (en) 2008-07-25
KR101135134B1 (ko) 2012-04-13
HRP20120039T1 (hr) 2012-02-29
HRP20080197T3 (en) 2008-05-31
IS8415A (is) 2006-04-12
HK1085513A1 (en) 2006-08-25
CY1112657T1 (el) 2016-02-10
KR20060092218A (ko) 2006-08-22
NO20061373L (no) 2006-03-27
IS2583B (is) 2010-02-15
IL173688A (en) 2010-12-30
IL173688A0 (en) 2006-07-05

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