HRP20080197T3 - Method for linking sequences of interest - Google Patents

Method for linking sequences of interest

Info

Publication number
HRP20080197T3
HRP20080197T3 HR20080197T HRP20080197T HRP20080197T3 HR P20080197 T3 HRP20080197 T3 HR P20080197T3 HR 20080197 T HR20080197 T HR 20080197T HR P20080197 T HRP20080197 T HR P20080197T HR P20080197 T3 HRP20080197 T3 HR P20080197T3
Authority
HR
Croatia
Prior art keywords
interest
sequences
variable region
nucleotide sequence
binding
Prior art date
Application number
HR20080197T
Other languages
Croatian (hr)
Inventor
B. Oleksiewicz Martin
S. Nielsen Lars
S. Andersen Peter
H. Hansen Margit
Original Assignee
Symphogen A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/DK2004/000633 external-priority patent/WO2005042774A2/en
Application filed by Symphogen A/S filed Critical Symphogen A/S
Publication of HRP20080197T3 publication Critical patent/HRP20080197T3/en
Publication of HRP20080197T8 publication Critical patent/HRP20080197T8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Abstract

Postupak povezivanja više ne-susjednih nukleotidnih sekvenci od interesa, gdje spomenut postupak uključuje: a) amplifikaciju, u multipleksnom molekularnom amplifikacijskom postupku, nukleotidne sekvence od interesa rabeći predložak koji potječe od izolirane jedne stanice; teb) učinak na povezivanjenukleotidne sekvence od interesa amplificirane u koraku a), i gdje nukleotidna sekvenca od interesauključuje sekvence koje kodiraju za promjenjivo područje te da povezivanje stvara srodni par sekvenci koje kodiraju za promjenjivo područje. Patent sadrži još 32 patentna zahtjeva.A method of linking multiple non-adjacent nucleotide sequences of interest, wherein said method includes: a) amplifying, in a multiplex molecular amplification method, nucleotide sequences of interest using a template originating from an isolated single cell; teb) an effect on the binding of a nucleotide sequence of interest amplified in step a), and wherein the nucleotide sequence of interest includes sequences encoding for a variable region and that the binding produces a related pair of sequences encoding for a variable region. The patent contains 32 additional claims.

HR20080197T 2003-09-18 2008-04-30 Method for linking sequences of interest HRP20080197T8 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US50458903P 2003-09-18 2003-09-18
US50445503P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15
PCT/DK2004/000633 WO2005042774A2 (en) 2003-09-18 2004-09-17 Method for linking sequences of interest

Publications (2)

Publication Number Publication Date
HRP20080197T3 true HRP20080197T3 (en) 2008-05-31
HRP20080197T8 HRP20080197T8 (en) 2008-07-31

Family

ID=34199056

Family Applications (2)

Application Number Title Priority Date Filing Date
HR20080197T HRP20080197T8 (en) 2003-09-18 2008-04-30 Method for linking sequences of interest
HR20120039T HRP20120039T1 (en) 2003-09-18 2012-01-12 Method for linking sequences of interest

Family Applications After (1)

Application Number Title Priority Date Filing Date
HR20120039T HRP20120039T1 (en) 2003-09-18 2012-01-12 Method for linking sequences of interest

Country Status (7)

Country Link
KR (1) KR101135134B1 (en)
CY (2) CY1108119T1 (en)
HK (2) HK1085513A1 (en)
HR (2) HRP20080197T8 (en)
IL (1) IL173688A (en)
IS (1) IS2583B (en)
NO (1) NO20061373L (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0597960B1 (en) * 1991-08-10 1999-01-20 Medical Research Council Treatment of cell populations

Also Published As

Publication number Publication date
IL173688A0 (en) 2006-07-05
IL173688A (en) 2010-12-30
HRP20080197T8 (en) 2008-07-31
HK1110893A1 (en) 2008-07-25
CY1108119T1 (en) 2014-02-12
NO20061373L (en) 2006-03-27
CY1112657T1 (en) 2016-02-10
IS8415A (en) 2006-04-12
KR20060092218A (en) 2006-08-22
IS2583B (en) 2010-02-15
HRP20120039T1 (en) 2012-02-29
KR101135134B1 (en) 2012-04-13
HK1085513A1 (en) 2006-08-25

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