CY1112657T1 - CONNECTION METHOD OF INTERFERENCE CHAINS - Google Patents

CONNECTION METHOD OF INTERFERENCE CHAINS

Info

Publication number
CY1112657T1
CY1112657T1 CY20121100200T CY121100200T CY1112657T1 CY 1112657 T1 CY1112657 T1 CY 1112657T1 CY 20121100200 T CY20121100200 T CY 20121100200T CY 121100200 T CY121100200 T CY 121100200T CY 1112657 T1 CY1112657 T1 CY 1112657T1
Authority
CY
Cyprus
Prior art keywords
chains
allows
libraries
interference
cell receptors
Prior art date
Application number
CY20121100200T
Other languages
Greek (el)
Inventor
Martin B Oleksiewicz
Lars S Nielsen
Peter S Andersen
Margit H Hansen
Original Assignee
Symphogen A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP08152066A external-priority patent/EP1921144B1/en
Application filed by Symphogen A/S filed Critical Symphogen A/S
Publication of CY1112657T1 publication Critical patent/CY1112657T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Η πολλαπλή RΤ-ΡCR υπερκάλυψης-επέκτασης παρέχει μία αποτελεσματική μέθοδο σύνδεσης δύο ή περισσότερων αλυσίδων νουκλεοτιδίων (nucleotide) που κωδικοποιούν περιοχές ή υπομονάδες μιας ετερομερούς πρωτεΐνης, σε μία απλή αντίδραση. Ειδικότερα η σύνδεση των αλυσίδων κωδικοποίησης από π.χ. ανοσογλοβουλίνες, λήπτες Τ κυττάρων ή λήπτες Β κυττάρων διευκολύνεται με την μέθοδο της παρούσας εφεύρεσης. Αυτό επιτρέπει πιο αποτελεσματικό τρόπο δημιουργίας βιβλιοθηκών αλυσίδων κωδικοποίησης μεταβλητής περιοχής. Η ικανότητα πραγματοποίησης πολλαπλής RΤ-ΡCR υπερκάλυψης-επέκτασης χρησιμοποιώντας μία φόρμα που παράγεται από ένα απομονωμένο ξεχωριστό κύτταρο επιτρέπει την δημιουργία βιβλιοθηκών συγγενών ζευγών σε μία μορφή υψηλής παραγωγής.Multiple RT-PCR overlap-extension provides an efficient method of linking two or more nucleotide chains encoding regions or subunits of a heteromeric protein, in a single reaction. In particular the connection of coding chains from e.g. immunoglobulins, T cell receptors or B cell receptors are facilitated by the method of the present invention. This allows for a more efficient way of creating variable region coding library libraries. The ability to perform multiple RT-PCR overlap-extension using a form produced by an isolated individual cell allows the generation of related pairs of libraries in a high output form.

CY20121100200T 2003-09-18 2012-02-24 CONNECTION METHOD OF INTERFERENCE CHAINS CY1112657T1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US50445503P 2003-09-18 2003-09-18
US50458903P 2003-09-18 2003-09-18
DKPA200301867 2003-12-17
DKPA200400782 2004-05-15
EP08152066A EP1921144B1 (en) 2003-09-18 2004-09-17 Method for linking sequences of interest

Publications (1)

Publication Number Publication Date
CY1112657T1 true CY1112657T1 (en) 2016-02-10

Family

ID=34199056

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20081100597T CY1108119T1 (en) 2003-09-18 2008-06-05 CONNECTION METHOD OF INTERFERENCE CHAINS
CY20121100200T CY1112657T1 (en) 2003-09-18 2012-02-24 CONNECTION METHOD OF INTERFERENCE CHAINS

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CY20081100597T CY1108119T1 (en) 2003-09-18 2008-06-05 CONNECTION METHOD OF INTERFERENCE CHAINS

Country Status (7)

Country Link
KR (1) KR101135134B1 (en)
CY (2) CY1108119T1 (en)
HK (2) HK1085513A1 (en)
HR (2) HRP20080197T8 (en)
IL (1) IL173688A (en)
IS (1) IS2583B (en)
NO (1) NO20061373L (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019013096A2 (en) * 2016-12-23 2019-12-17 Visterra Inc ligand polypeptides and methods of preparing them

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE175997T1 (en) * 1991-08-10 1999-02-15 Medical Res Council TREATMENT OF CELL POPULATIONS

Also Published As

Publication number Publication date
HK1110893A1 (en) 2008-07-25
IL173688A (en) 2010-12-30
IS2583B (en) 2010-02-15
HRP20080197T3 (en) 2008-05-31
CY1108119T1 (en) 2014-02-12
IS8415A (en) 2006-04-12
KR20060092218A (en) 2006-08-22
KR101135134B1 (en) 2012-04-13
HK1085513A1 (en) 2006-08-25
HRP20120039T1 (en) 2012-02-29
HRP20080197T8 (en) 2008-07-31
NO20061373L (en) 2006-03-27
IL173688A0 (en) 2006-07-05

Similar Documents

Publication Publication Date Title
DK1921144T3 (en) Method of linking sequences of interest
CY1119641T1 (en) METHODS OF CONSTRUCTION OF IMMOBILINES
CY1117537T1 (en) Mice Expressing a Hybrid Lightweight Chain of Immunoglobulin
DK2121920T3 (en) Method for cloning related antibodies
CY1114639T1 (en) CHEMICAL SINGLE-DIMENSIONAL HYBRID HYBRIDS
CY1107669T1 (en) SIMPLE CHAIN VHH CAMELOID ANTIBODIES AND METHOD FOR PREPARING IT IN A Mammal
RS54468B1 (en) Monoclonal antibodies against claudin-18 for treatment of cancer
CY1113400T1 (en) CHANGE OF CONNECTION CONNECTIONS WITH FCRN OR TRANSFERRED HALF-TERM HALF
CY1109692T1 (en) NEW IMMUNE REACTIONS
WO2007148337A3 (en) Programmable iterated elongation: a method for manufacturing synthetic genes and combinatorial dna and protein libraries
CY1112754T1 (en) METHODS AND INTERMEDIATES FOR THE PRODUCTION OF N4-FENYL-KINAZOLIN-4-AMINE PRODUCERS
CY1111151T1 (en) CELL SOLUTION A2 PHOSPHOLISING INHIBITIONS
BRPI0917352A2 (en) METHODS FOR PRODUCING A LIBRARY OF COGNATE PAIRS COMPRISING LINKED SEQUENCES ENCODING VARIABLE REGIONS AND FOR GENERATING A VECTOR ENCODING A CHIMERIC ANTIBODY WITH CONSTANT HUMAN REGIONS AND NON-HUMAN VARIABLE REGIONS, VECTOR LIBRARY, AND METHOD FOR PRODUCING A LIBRARY OF AVIAN-DERIVED IMMUNOGLOBULIN SEQUENCES
CY1112888T1 (en) METHOD OF PREPARATION OF (S) -PANTOPRAZOL
DK1800133T3 (en) Methods for screening antibody libraries
CY1112657T1 (en) CONNECTION METHOD OF INTERFERENCE CHAINS
ATE414795T1 (en) ANTIBODIES LIBRARIES
CY1105675T1 (en) PROTEIN ROSDENOUS ANGIOSTATIN
IL158984A0 (en) Use of duckweed in high throughput screening
ATE443083T1 (en) CATION-CONDUCTING GABA-A RECEPTORS AND THEIR USE
PA8612801A1 (en) METHOD FOR LINKING INTEREST SEQUENCES
EA202192299A1 (en) VARIANT NUCLEIC ACID LIBRARIES FOR ANTIBODY OPTIMIZATION
Chrysostomou Addressing intrinsic challenges for next generation sequencing of immunoglobulin repertoires.
CY1115280T1 (en) Gene Expression Regulatory Method
CY1113852T1 (en) OPTIMIZED ANTIBODIES TARGETING TO CD19