EA200401407A1 - Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения - Google Patents

Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения

Info

Publication number
EA200401407A1
EA200401407A1 EA200401407A EA200401407A EA200401407A1 EA 200401407 A1 EA200401407 A1 EA 200401407A1 EA 200401407 A EA200401407 A EA 200401407A EA 200401407 A EA200401407 A EA 200401407A EA 200401407 A1 EA200401407 A1 EA 200401407A1
Authority
EA
Eurasian Patent Office
Prior art keywords
phospholipases
nucleic acids
acids encoding
activity
methods
Prior art date
Application number
EA200401407A
Other languages
English (en)
Other versions
EA010903B1 (ru
Inventor
Светлана Граматикова
Джеофф Хэйзлвуд
Дэвид Лэм
Нельсон Бартон
Original Assignee
Дайверса Корпорейшн
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дайверса Корпорейшн filed Critical Дайверса Корпорейшн
Publication of EA200401407A1 publication Critical patent/EA200401407A1/ru
Publication of EA010903B1 publication Critical patent/EA010903B1/ru

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к новым фосфолипазам, обладающим фосфолипазной активностью, например активностью фосфолипазы A, B, C и D, активностью пататина, активностью ацилгидролазы липидов (АГЛ), к нуклеиновым кислотам, кодирующим их, и к антителам, которые связываются с ними. Описаны также промышленные способы, например дегумирование масла, и продукты, включающие использование данных фосфолипаз.Отчет о международном поиске был опубликован 2004.10.14.
EA200401407A 2002-04-19 2003-04-21 Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения EA010903B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37431302P 2002-04-19 2002-04-19
PCT/US2003/012556 WO2003089620A2 (en) 2002-04-19 2003-04-21 Phospholipases, nucleic acids encoding them and methods for making and using them

Publications (2)

Publication Number Publication Date
EA200401407A1 true EA200401407A1 (ru) 2006-02-24
EA010903B1 EA010903B1 (ru) 2008-12-30

Family

ID=29251173

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200401407A EA010903B1 (ru) 2002-04-19 2003-04-21 Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения

Country Status (11)

Country Link
US (1) US20040005604A1 (ru)
EP (2) EP1497418B1 (ru)
JP (1) JP2005523019A (ru)
CN (2) CN102174546A (ru)
AU (1) AU2003243157C1 (ru)
BR (2) BR0309391A (ru)
CA (1) CA2481411C (ru)
EA (1) EA010903B1 (ru)
ES (1) ES2395898T3 (ru)
PT (1) PT1497418E (ru)
WO (1) WO2003089620A2 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2818353C2 (ru) * 2018-12-28 2024-05-02 УИЛМАР (ШАНХАЙ) БАЙОТЕКНОЛОДЖИ РИСЕРЧ Энд ДИВЕЛОПМЕНТ СЕНТЕР КО., ЛТД Мутант фосфолипазы С с высокой ферментативной активностью

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
US7943360B2 (en) 2002-04-19 2011-05-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
CA2515583C (en) 2003-03-07 2015-07-14 Diversa Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
DE10355838A1 (de) * 2003-11-26 2005-07-07 Martin-Luther-Universität Halle-Wittenberg Verfahren zur Herstellung von Phospholipase A2
AU2011218662B2 (en) * 2004-03-08 2013-01-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
JP2008505619A (ja) * 2004-06-16 2008-02-28 ヴェレニウム コーポレイション 葉緑素を酵素的に脱色するための組成物及び方法
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
EP1791933B1 (en) 2004-07-16 2011-06-29 Danisco A/S Enzymatic oil-degumming method
US8557551B2 (en) 2004-09-10 2013-10-15 Dsm Ip Assets B.V. Compositions and methods for making and modifying oils
EP1910530A2 (en) * 2005-05-31 2008-04-16 Diversa Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
US20070148311A1 (en) * 2005-12-22 2007-06-28 Bunge Oils, Inc. Phytosterol esterification product and method of make same
EP2216403A3 (en) * 2006-02-02 2010-11-24 Verenium Corporation Esterases and related nucleic acids and methods
MX2009003034A (es) * 2006-09-21 2009-11-18 Verenium Corp Fosfolipasas, acidos nucleicos que las codifican, y metodos de hacerlas y usarlas.
US20080235824A1 (en) * 2006-12-17 2008-09-25 Gabriel Dean W Use of Esterase Genes as Selectable Markers for Transforming Plant Cells
CN101610824A (zh) * 2006-12-22 2009-12-23 丹尼斯科美国公司 酶辅助的水性脂类提取物的脱乳化作用
US8460905B2 (en) * 2007-09-11 2013-06-11 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time
US8956853B2 (en) * 2007-01-30 2015-02-17 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases
ITRM20070129A1 (it) * 2007-03-14 2008-09-15 Plantechno S R L Piante di tabacco mutagenizzate come coltura da seme per la produzioe di olio da usare ai fini energetici industriali e alimentari
JP5302189B2 (ja) * 2007-04-27 2013-10-02 長瀬産業株式会社 スフィンゴミエリナーゼ
CA2699406C (en) 2007-09-12 2019-09-03 Martek Biosciences Corporation Biological oils and production and uses thereof
US8076123B2 (en) * 2007-10-26 2011-12-13 Oilseeds Biorefinery Corporation Emulsification-free degumming of oil
JP5509094B2 (ja) * 2007-12-21 2014-06-04 デュポン ニュートリション バイオサイエンシーズ エーピーエス 脂質アシルトランスフェラーゼを用いる食用油精製のためのプロセス
US8241876B2 (en) 2008-01-07 2012-08-14 Bunge Oils, Inc. Generation of triacylglycerols from gums
UA111708C2 (uk) * 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
WO2011082190A1 (en) 2009-12-28 2011-07-07 Martek Biosciences Corporation Recombinant thraustochytrids that grow on sucrose, and compositions, methods of making, and uses thereof
CN102906270B (zh) * 2009-12-28 2016-06-22 Dsmip资产公司 在木糖上生长的重组破囊壶菌和其组合物、制备方法及用途
AR080293A1 (es) 2010-03-12 2012-03-28 Danisco Proceso de refinamiento de un aceite vegetal
MX2012014685A (es) 2010-06-17 2013-02-11 Dupont Nutrition Biosci Aps Proceso.
KR101202135B1 (ko) 2010-06-22 2012-11-15 고려대학교 산학협력단 병저항성 관련 고추 파타틴 유사 포스포라이페이즈 유전자 CaPLP1 및 이를 이용한 식물병 저항성 탐색 및 형질전환 식물체
DE102010025764A1 (de) * 2010-07-01 2012-01-05 Süd-Chemie AG Phospholipase-Träger-Komplex
ES2649912T3 (es) 2011-02-23 2018-01-16 Dupont Nutrition Biosciences Aps Tratamiento enzimático de la clorofila en los aceites vegetales
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
CN102504946B (zh) * 2011-11-04 2013-09-04 福建春辉生物工程有限公司 一种对三高人群有益的油茶籽毛油精炼方法
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
RU2500811C1 (ru) * 2012-10-10 2013-12-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") Рекомбинантный штамм бактерий bacillus subtilis - продуцент фосфолипазы с
WO2014160862A1 (en) 2013-03-27 2014-10-02 The Curators Of The University Of Missouri Increasing the content of long chain fatty acids in seed oil
CN103540576B (zh) * 2013-09-30 2015-05-20 上海交通大学 patatin-like磷脂酶及其编码基因与用途
CN104630176B (zh) * 2013-11-13 2020-05-01 丰益(上海)生物技术研发中心有限公司 一种提高发酵法生产磷脂酶c产量的方法
CN104726357B (zh) * 2013-12-20 2019-07-09 丰益(上海)生物技术研发中心有限公司 一株产生磷脂酶的解淀粉芽孢杆菌诱变菌株及磷脂酶c
US10351795B2 (en) 2014-03-19 2019-07-16 Novozymes A/S Polypeptides having phospholipase C activity and polynucleotides encoding same
EP3143135B1 (en) 2014-05-15 2019-04-10 Novozymes A/S Compositions comprising polypeptides having phospholipase c activity and use thereof
CN104604704A (zh) * 2015-02-16 2015-05-13 安徽科技学院 一种b5(含碳源)固体培养基质的规模化制作方法
CA2980261A1 (en) 2015-04-14 2016-10-20 Dsm Ip Assets B.V. Use of phospholipase c
ES2913437T3 (es) 2016-02-19 2022-06-02 Basf Se Lipasas para hornear
WO2017151103A1 (en) * 2016-02-29 2017-09-08 Hewlett-Packard Development Company, L.P. Enzymatic sample purification
CN105567713B (zh) * 2016-03-09 2019-01-08 河北省农林科学院粮油作物研究所 花生AhPLDα3蛋白及其编码基因与应用
CN110129299B (zh) * 2016-06-02 2021-08-03 天津科技大学 磷脂酶d及其应用
CN108220267B (zh) * 2016-12-22 2022-10-04 丰益(上海)生物技术研发中心有限公司 磷脂酶及其应用
EP3621984A4 (en) 2017-05-12 2021-03-03 Basf Se METHOD OF USING LIPASE ENZYMES FOR CLEANING
US11542487B2 (en) 2017-10-25 2023-01-03 Basf Se Beta-amylase enzymes
CN108384768A (zh) * 2018-02-28 2018-08-10 江苏中酶生物科技有限公司 一种磷脂酶c的突变体及其应用
EP3784779A1 (en) 2018-04-26 2021-03-03 Basf Se Lipase enzymes
US20210115422A1 (en) 2018-05-03 2021-04-22 Basf Se Amylase enzymes
CN111378634B (zh) * 2018-12-28 2024-04-02 丰益(上海)生物技术研发中心有限公司 具有磷脂酶c活性的多肽及其用途
CN111051505B (zh) * 2018-12-29 2023-11-03 邦泰生物工程(深圳)有限公司 一种磷脂酶d突变体、其应用及其制备磷酯酰丝氨酸的方法
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
MX2021010110A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio.
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
US20220170001A1 (en) 2019-03-25 2022-06-02 Basf Se Amylase Enzymes
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
US20220162576A1 (en) 2019-03-25 2022-05-26 Basf Se Amylase enzymes
EP3983425A1 (en) 2019-06-13 2022-04-20 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
PL3959326T3 (pl) 2019-07-05 2023-10-09 Basf Se Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem
JP2022545465A (ja) 2019-08-22 2022-10-27 ビーエーエスエフ ソシエタス・ヨーロピア アミラーゼバリアント
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114835780B (zh) * 2022-06-29 2022-09-27 中国农业大学 辣椒籽分离的寡肽medei及其在预防或治疗癌症中的应用

Family Cites Families (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US87571A (en) 1869-03-09 Improved axle-set
SE318048B (ru) 1959-09-21 1969-12-01 Pellerin Ab Zenith
SE417441B (sv) 1977-08-19 1981-03-16 Kare Larsson Forfarande for att isolera polera lipider ur en blandning av polera och opolera cerealiclipider genom blandning med vatten och derpa foljande tyngdkraftsseparation
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4707364A (en) * 1984-01-27 1987-11-17 Miles Laboratories, Inc. Composition for accelerating cheese aging
US5087571A (en) 1984-06-22 1992-02-11 President And Fellows Of Harvard College Method for providing a cell culture from a transgenic non-human mammal
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE3587814T2 (de) 1985-03-30 1994-11-10 Marc Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US4752483A (en) * 1986-05-30 1988-06-21 Campbell Soup Company Method for producing a highly flavored cheese ingredient
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5573933A (en) 1987-04-14 1996-11-12 Luminis Pty, Ltd. Transgenic pigs
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5217879A (en) 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US5326477A (en) 1990-05-07 1994-07-05 Bio-Sep, Inc. Process for digesting solid waste
DE69133372T2 (de) 1990-06-15 2005-02-24 Scios Inc., Sunnyvale Transgenes, nicht-menschliches säugetier das die amyloidbildende pathologie der alzheimerschen krankheit zeigt
EP0558676B1 (en) 1990-11-23 2000-04-19 Plant Genetic Systems, N.V. Process for transforming monocotyledonous plants
CA2098824A1 (en) 1990-12-20 1992-06-21 William D. Huse Optimization of binding proteins
US6110700A (en) 1991-03-11 2000-08-29 The General Hospital Corporation PRAD1 cyclin and its cDNA
DE4115938A1 (de) 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
US5922854A (en) 1991-06-14 1999-07-13 Kumar; Ramesh Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids
CA2075135A1 (en) 1991-08-02 1993-02-03 David E. Ellis Particle-mediated transformation of gymnosperms
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
JP2626380B2 (ja) 1991-11-12 1997-07-02 株式会社大林組 クレーン用吊治具の旋回防止装置
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
JP2937746B2 (ja) 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法
JPH06306386A (ja) 1993-04-25 1994-11-01 Showa Sangyo Co Ltd 油脂の精製方法
WO1995008914A1 (en) 1993-09-30 1995-04-06 Agracetus, Inc. Transgenic cotton plants producing heterologous peroxidase
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
DE4339556C1 (de) 1993-11-19 1995-02-02 Metallgesellschaft Ag Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5639940A (en) 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
WO1995024488A1 (en) 1994-03-09 1995-09-14 Abbott Laboratories Transgenic animals producing oligosaccharides and glycoconjugates
NZ279676A (en) 1994-03-09 1998-04-27 Abbott Lab Humanized milk produced by non-human transgenic mammal transformed with a heterologous gene coding for human enzyme producing human oligosaccharides and glycoconjugates
US5824531A (en) 1994-03-29 1998-10-20 Novid Nordisk Alkaline bacilus amylase
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5959171A (en) 1994-08-17 1999-09-28 Pharming B.V. Method for the production of biologically active polypeptides in a mammal's
WO1996006927A1 (en) 1994-09-01 1996-03-07 Merck & Co., Inc. Transgenic animal expressing a familial form of human amyloid precursor protein
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US6187992B1 (en) 1994-12-05 2001-02-13 Merck & Co., Inc. Transgenic mouse having a disrupted amyloid precursor protein gene
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
EP0831854A4 (en) 1995-06-06 2001-01-24 Isis Pharmaceuticals Inc OLIGONUCLEOTIDS WITH PHOSPHOROTHIOATE BINDINGS OF HIGH CHIRAL PURITY
PL324288A1 (en) 1995-06-27 1998-05-11 Unilever Nv Immobilised enzyme and application thereof in transformation of triglyceride oils
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
DE19527274A1 (de) 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
SE504664C2 (sv) 1995-09-22 1997-03-24 Scotia Lipidteknik Ab Sätt att framställa fraktionerad olja, oljan, dess användning samt emulsionskomposition innehållande oljan
WO1997016533A1 (en) 1995-10-31 1997-05-09 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6022963A (en) 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
AU3568897A (en) 1996-06-07 1998-01-05 Eos Biotechnology, Inc. Immobilised linear oligonucleotide arrays
US6326341B1 (en) 1996-09-11 2001-12-04 The Procter & Gamble Company Low foaming automatic dishwashing compositions
WO1998018912A1 (en) 1996-10-31 1998-05-07 Novo Nordisk A/S Novel phospholipase, production and use thereof
US6127137A (en) 1996-10-31 2000-10-03 Novo Nordisk A/S Acidic phospholipase, production and methods using thereof
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
JP3824174B2 (ja) 1996-12-09 2006-09-20 ノボザイムス アクティーゼルスカブ ホスホリパーゼの使用による高い量の非水和性リンを含む食用油中のリン含有成分の減少、ホスホリパーゼaおよび/またはb活性を有する糸状菌からのホスホリパーゼ
WO1998027199A1 (en) 1996-12-19 1998-06-25 Unilever N.V. Immobilized enzyme and its use for the processing of triglyceride oils
US6069122A (en) 1997-06-16 2000-05-30 The Procter & Gamble Company Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE69835360T2 (de) 1997-01-17 2007-08-16 Maxygen, Inc., Redwood City EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US6303803B1 (en) 1997-01-31 2001-10-16 Cargill, Incorporated Removal of sterols from fats and oils
US5880300A (en) 1997-01-31 1999-03-09 Cargill, Incorporated Phospholipid-based removal of sterols from fats and oils
EP0996718B1 (en) 1997-03-18 2008-12-24 Novozymes A/S Method for constructing a library using dna shuffling
BR9808368A (pt) 1997-03-18 2000-05-23 Novo Nordisk As Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse.
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6826296B2 (en) 1997-07-25 2004-11-30 Affymetrix, Inc. Method and system for providing a probe array chip design database
EP1012335A4 (en) 1997-08-15 2004-06-09 Hyseq Inc METHODS AND COMPOSITIONS FOR DETECTION OR QUANTIFICATION OF NUCLEIC ACID SPECIES
CN1249418C (zh) 1997-09-11 2006-04-05 生物风险公司 一种生产目标物质的高密度阵列的方法及高密度阵列
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
WO1999021979A1 (en) 1997-10-28 1999-05-06 Maxygen, Inc. Human papillomavirus vectors
EP2386568B1 (en) 1997-10-30 2014-08-06 Novozymes A/S Alpha-amylase mutants
EP1690868A1 (en) 1997-10-31 2006-08-16 Maxygen, Inc. Modification of virus tropism and host range by viral genome shuffling
DE69812403T2 (de) 1997-11-10 2004-01-29 Procter & Gamble Verfahren zur herstellung einer waschmitteltablette
US6118044A (en) 1997-11-14 2000-09-12 Sankyo Company, Limited Transgenic animal allergy models and methods for their use
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AU3289199A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Antigen library immunization
AU3291099A (en) 1998-02-11 1999-08-30 Maxygen, Inc. Genetic vaccine vector engineering
WO1999051773A1 (en) 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
US6156952A (en) 1998-04-09 2000-12-05 Constituent Institution Of The University Of Maryland System HIV transgenic animals and uses therefor
WO1999057360A1 (en) 1998-05-01 1999-11-11 Novo Nordisk A/S Enhancers such as n-hydroxyacetanilide
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
DE19824705A1 (de) 1998-06-03 1999-12-09 Henkel Kgaa Amylase und Protease enthaltende Wasch- und Reinigungsmittel
IL140100A0 (en) 1998-06-29 2002-02-10 Phylos Inc Methods of generating highly diverse libraries
JP4335995B2 (ja) 1998-07-22 2009-09-30 昭 神谷 環境保全型粒状洗浄用組成物
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US6172248B1 (en) * 1998-11-20 2001-01-09 Ip Holdings, L.L.C. Methods for refining vegetable oils and byproducts thereof
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
US6511824B1 (en) 1999-03-17 2003-01-28 Exelixis, Inc. Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use
US6309871B1 (en) 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US20010008765A1 (en) 1999-12-06 2001-07-19 Fuji Photo Film Co., Ltd. DNA chip and reactive solid carrier
US6531644B1 (en) 2000-01-14 2003-03-11 Exelixis, Inc. Methods for identifying anti-cancer drug targets
AU1166502A (en) 2000-10-12 2002-04-22 Exelixis Inc Human ect2 and methods of use
US6309872B1 (en) 2000-11-01 2001-10-30 Novozymes Biotech, Inc Polypeptides having glucoamylase activity and nucleic acids encoding same
SG11201510439PA (en) 2013-06-21 2016-01-28 Bio Rad Laboratories Microfluidic system with fluid pickups

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2818353C2 (ru) * 2018-12-28 2024-05-02 УИЛМАР (ШАНХАЙ) БАЙОТЕКНОЛОДЖИ РИСЕРЧ Энд ДИВЕЛОПМЕНТ СЕНТЕР КО., ЛТД Мутант фосфолипазы С с высокой ферментативной активностью
RU2819269C2 (ru) * 2018-12-28 2024-05-16 УИЛМАР (ШАНХАЙ) БАЙОТЕКНОЛОДЖИ РИСЕРЧ Энд ДИВЕЛОПМЕНТ СЕНТЕР КО., ЛТД Полипептид, обладающий активностью фосфолипазы С, и его применение

Also Published As

Publication number Publication date
ES2395898T3 (es) 2013-02-15
WO2003089620A3 (en) 2004-10-14
CA2481411A1 (en) 2003-10-30
PT1497418E (pt) 2013-01-25
JP2005523019A (ja) 2005-08-04
EP2298871A1 (en) 2011-03-23
EP1497418B1 (en) 2012-10-17
WO2003089620A2 (en) 2003-10-30
EA010903B1 (ru) 2008-12-30
EP1497418A4 (en) 2006-02-01
US20040005604A1 (en) 2004-01-08
AU2003243157B2 (en) 2008-03-20
CA2481411C (en) 2016-06-14
BR0309391A (pt) 2005-10-25
EP1497418A2 (en) 2005-01-19
CN1659276A (zh) 2005-08-24
CN102174546A (zh) 2011-09-07
AU2003243157A1 (en) 2003-11-03
BRPI0309391B1 (pt) 2018-10-30
AU2003243157C1 (en) 2008-09-04

Similar Documents

Publication Publication Date Title
EA200401407A1 (ru) Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения
EA200970308A1 (ru) Фосфолипазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
WO2005086900A3 (en) Phospholipases, nucleic acids encoding them and methods for making and using them
ES2183113T3 (es) Reduccion de los componentes que contienen fosforo en los aceites comestibles que comprenden una cantidad elevada de fosforo no hidratable mendiante el uso de una fosfolipasa, una fosfolipasa de un hongo filamentoso que tiene una actividad de fosfolipasa a y/o b.
BR112012008957A2 (pt) fosfolipases, ácidos nucleicos que codificam os mesmos e métodos para fazer e usar os mesmo
MY194898A (en) Oil degumming methods
EA200100691A1 (ru) Пищевой продукт, содержащий пузырьки газа
EA200001167A1 (ru) Новые комбинации генов, изменяющие качество и функциональность соевого масла
Butt et al. Postfractionation for enhanced proteomic analyses: routine electrophoretic methods increase the resolution of standard 2D-PAGE
EA199901027A1 (ru) Сложные моноэфиры пробукола для лечения сердечно-сосудистых и воспалительных заболеваний
DE69613373D1 (de) Methode zur herstellung von schmierölen
PT941066E (pt) Complexos de lipido cationico - acido nucleico
EA200000868A1 (ru) Ингибиторы фосфолипаз
DE69133602D1 (de) Pflanzliche Thioesterasen
ATE403749T1 (de) Ladungsmarker und auftrennung von nukleinsäuremolekülen
DE59610388D1 (de) Enzymatisches verfahren zur entschleimung von pflanzlichen ölen mit aspergillus-phospholipase
EA200000765A1 (ru) Получение липидной смеси и фосфолипидная суспензия, содержащая липидную смесь
ATE431402T1 (de) Protease, ein gen dafür und anwendung davon
UY27606A1 (es) Inhibidores de la fosfarasa
DE60214537D1 (de) Entfernung von ölen aus säuren
ATE273313T1 (de) Verfahren zur herstellung von reinen phosphatiden und deren verwendung in der kosmetik, im pharmazeutischen gebiet und im bereich der nahrungsmittel
Ferreira de Oliveira et al. Two-dimensional blue native/SDS polyacrylamide gel electrophoresis for analysis of Brazilian Bothrops snake venoms
EA200000851A1 (ru) Е - 2 - [4 - (4 - хлор - 1, 2 - дифенил - бут - 1 - енил) фенокси] этанол и фармацевтические композиции на его основе
DE69511130T2 (de) Verfahren zur Herstellung von Basisschmieröl
EA199800843A1 (ru) Компьютерный способ создания химических структур, имеющих общие функциональные характеристики

Legal Events

Date Code Title Description
PD4A Registration of transfer of a eurasian patent in accordance with the succession in title
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

PC4A Registration of transfer of a eurasian patent by assignment
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU