BR0309391A - Fosfolipases, ácidos nucléicos codificando-as e métodos para preparar e usá-las - Google Patents

Fosfolipases, ácidos nucléicos codificando-as e métodos para preparar e usá-las

Info

Publication number
BR0309391A
BR0309391A BR0309391-3A BR0309391A BR0309391A BR 0309391 A BR0309391 A BR 0309391A BR 0309391 A BR0309391 A BR 0309391A BR 0309391 A BR0309391 A BR 0309391A
Authority
BR
Brazil
Prior art keywords
phospholipases
nucleic acids
acids encoding
methods
preparing
Prior art date
Application number
BR0309391-3A
Other languages
English (en)
Inventor
Svetlana Gramatikova
Geoff Hazelwood
David Lam
Nelson Barton
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp filed Critical Diversa Corp
Publication of BR0309391A publication Critical patent/BR0309391A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

"FOSFOLIPASES, áCIDOS NUCLéICOS CODIFICANDO-AS E MéTODOS PARA PREPARAR E USá-LAS". A invenção fornece novos polipeptídeos tendo atividade de fosfolipase, incluindo, por exemplo, atividade de fosfolipase A, B, C e D, atividade de patatina, atividade de lipídeo de acil hidrolase (LAH), ácidos nucléicos codificando-os e anticorpos que ligam-se a eles. Métodos industriais, por exemplo, desengomamento de óleo, e produtos compreendendo o emprego destas fosfolipases são também fornecidos.
BR0309391-3A 2002-04-19 2003-04-21 Fosfolipases, ácidos nucléicos codificando-as e métodos para preparar e usá-las BR0309391A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37431302P 2002-04-19 2002-04-19
PCT/US2003/012556 WO2003089620A2 (en) 2002-04-19 2003-04-21 Phospholipases, nucleic acids encoding them and methods for making and using them

Publications (1)

Publication Number Publication Date
BR0309391A true BR0309391A (pt) 2005-10-25

Family

ID=29251173

Family Applications (2)

Application Number Title Priority Date Filing Date
BR0309391-3A BR0309391A (pt) 2002-04-19 2003-04-21 Fosfolipases, ácidos nucléicos codificando-as e métodos para preparar e usá-las
BRPI0309391A BRPI0309391B1 (pt) 2002-04-19 2003-04-21 vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados

Family Applications After (1)

Application Number Title Priority Date Filing Date
BRPI0309391A BRPI0309391B1 (pt) 2002-04-19 2003-04-21 vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados

Country Status (11)

Country Link
US (1) US20040005604A1 (pt)
EP (2) EP1497418B1 (pt)
JP (1) JP2005523019A (pt)
CN (2) CN1659276A (pt)
AU (1) AU2003243157C1 (pt)
BR (2) BR0309391A (pt)
CA (1) CA2481411C (pt)
EA (1) EA010903B1 (pt)
ES (1) ES2395898T3 (pt)
PT (1) PT1497418E (pt)
WO (1) WO2003089620A2 (pt)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
ATE362315T1 (de) 2001-05-18 2007-06-15 Danisco Verfahren zur herstellung von teig mit einem enzym
US7226771B2 (en) * 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7943360B2 (en) 2002-04-19 2011-05-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
AU2004277368B2 (en) 2003-03-07 2011-01-20 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and mehods for making and using them
DE10355838A1 (de) * 2003-11-26 2005-07-07 Martin-Luther-Universität Halle-Wittenberg Verfahren zur Herstellung von Phospholipase A2
AU2011218662B2 (en) * 2004-03-08 2013-01-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
CN101432292B (zh) * 2004-06-16 2013-03-13 维莱尼姆公司 对叶绿素进行酶促脱色的组合物和方法
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
EP2267108A1 (en) 2004-07-16 2010-12-29 Danisco A/S Enzymatic oil-degumming method
WO2006031699A2 (en) 2004-09-10 2006-03-23 Diversa Corporation Compositions and methods for making and modifying oils
CA2611071A1 (en) * 2005-05-31 2007-05-18 Verenium Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
US20070148311A1 (en) * 2005-12-22 2007-06-28 Bunge Oils, Inc. Phytosterol esterification product and method of make same
EP1987142A4 (en) * 2006-02-02 2009-07-15 Verenium Corp ESTERASES AND ASSOCIATED NUCLEIC ACIDS AND METHODS
ES2451266T3 (es) * 2006-09-21 2014-03-26 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
WO2008076966A1 (en) * 2006-12-17 2008-06-26 Integrated Plant Genetcs, Inc. Use of esterase genes as selectable markers for transforming plant cells
EP2106280A2 (en) * 2006-12-22 2009-10-07 Danisco US, INC., Genencor Division Enzyme-assisted de-emulsification of aqueous lipid extracts
US8460905B2 (en) * 2007-09-11 2013-06-11 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time
US8956853B2 (en) * 2007-01-30 2015-02-17 Bunge Oils, Inc. Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases
ITRM20070129A1 (it) * 2007-03-14 2008-09-15 Plantechno S R L Piante di tabacco mutagenizzate come coltura da seme per la produzioe di olio da usare ai fini energetici industriali e alimentari
JP5302189B2 (ja) * 2007-04-27 2013-10-02 長瀬産業株式会社 スフィンゴミエリナーゼ
BRPI0815860B1 (pt) 2007-09-12 2021-04-20 Dsm Ip Assets B.V. métodos para produzir um óleo biológico
US8076123B2 (en) * 2007-10-26 2011-12-13 Oilseeds Biorefinery Corporation Emulsification-free degumming of oil
CN101978037B (zh) * 2007-12-21 2015-06-17 杜邦营养生物科学有限公司 使用脂质酰基转移酶精炼食用油的方法
US8241876B2 (en) 2008-01-07 2012-08-14 Bunge Oils, Inc. Generation of triacylglycerols from gums
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
CN103068965A (zh) 2009-12-28 2013-04-24 Dsmip资产公司 在蔗糖上生长的重组破囊壶菌和其组合物、制备方法及用途
ES2651313T3 (es) * 2009-12-28 2018-01-25 Dsm Ip Assets B.V. Traustoquítridos recombinantes que crecen en xilosa, y composiciones, métodos de preparación y usos de los mismos
AR080293A1 (es) 2010-03-12 2012-03-28 Danisco Proceso de refinamiento de un aceite vegetal
WO2011158203A1 (en) 2010-06-17 2011-12-22 Danisco A/S Process
KR101202135B1 (ko) 2010-06-22 2012-11-15 고려대학교 산학협력단 병저항성 관련 고추 파타틴 유사 포스포라이페이즈 유전자 CaPLP1 및 이를 이용한 식물병 저항성 탐색 및 형질전환 식물체
DE102010025764A1 (de) * 2010-07-01 2012-01-05 Süd-Chemie AG Phospholipase-Träger-Komplex
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
AR085250A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar un aceite vegetal
CN102504946B (zh) * 2011-11-04 2013-09-04 福建春辉生物工程有限公司 一种对三高人群有益的油茶籽毛油精炼方法
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
RU2500811C1 (ru) * 2012-10-10 2013-12-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") Рекомбинантный штамм бактерий bacillus subtilis - продуцент фосфолипазы с
WO2014160862A1 (en) * 2013-03-27 2014-10-02 The Curators Of The University Of Missouri Increasing the content of long chain fatty acids in seed oil
CN103540576B (zh) * 2013-09-30 2015-05-20 上海交通大学 patatin-like磷脂酶及其编码基因与用途
CN104630176B (zh) * 2013-11-13 2020-05-01 丰益(上海)生物技术研发中心有限公司 一种提高发酵法生产磷脂酶c产量的方法
CN104726357B (zh) * 2013-12-20 2019-07-09 丰益(上海)生物技术研发中心有限公司 一株产生磷脂酶的解淀粉芽孢杆菌诱变菌株及磷脂酶c
US10351795B2 (en) 2014-03-19 2019-07-16 Novozymes A/S Polypeptides having phospholipase C activity and polynucleotides encoding same
US20170058235A1 (en) 2014-05-15 2017-03-02 Novozymes A/S Compositions Comprising Polypeptides Having Phospholipase C Activity and Use Thereof
CN104604704A (zh) * 2015-02-16 2015-05-13 安徽科技学院 一种b5(含碳源)固体培养基质的规模化制作方法
CA2980261A1 (en) 2015-04-14 2016-10-20 Dsm Ip Assets B.V. Use of phospholipase c
CA3014326C (en) 2016-02-19 2024-01-09 Basf Se Baking lipases
US20190056299A1 (en) * 2016-02-29 2019-02-21 Hewlett-Packard Development Enzymatic sample purification
CN105567713B (zh) * 2016-03-09 2019-01-08 河北省农林科学院粮油作物研究所 花生AhPLDα3蛋白及其编码基因与应用
CN110129298B (zh) * 2016-06-02 2021-08-03 天津科技大学 一种磷脂酶d突变体及其应用
CN108220267B (zh) * 2016-12-22 2022-10-04 丰益(上海)生物技术研发中心有限公司 磷脂酶及其应用
WO2018209026A1 (en) 2017-05-12 2018-11-15 Basf Se Method for using lipase enzymes for cleaning
US11542487B2 (en) 2017-10-25 2023-01-03 Basf Se Beta-amylase enzymes
CN108384768A (zh) * 2018-02-28 2018-08-10 江苏中酶生物科技有限公司 一种磷脂酶c的突变体及其应用
CN112368375A (zh) 2018-04-26 2021-02-12 巴斯夫欧洲公司 脂肪酶
WO2019211143A1 (en) 2018-05-03 2019-11-07 Basf Se Amylase enzymes
CN111378634B (zh) * 2018-12-28 2024-04-02 丰益(上海)生物技术研发中心有限公司 具有磷脂酶c活性的多肽及其用途
CN111051505B (zh) * 2018-12-29 2023-11-03 邦泰生物工程(深圳)有限公司 一种磷脂酶d突变体、其应用及其制备磷酯酰丝氨酸的方法
MX2021010109A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos.
MX2021010110A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio.
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
EP3947664A2 (en) 2019-03-25 2022-02-09 Basf Se Amylase enzymes
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
US20220162576A1 (en) 2019-03-25 2022-05-26 Basf Se Amylase enzymes
US20220275355A1 (en) 2019-06-13 2022-09-01 Basf Se Method of Recovering a Protein from Fermentation Broth Using a Divalent Cation
PL3959326T3 (pl) 2019-07-05 2023-10-09 Basf Se Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem
CN114364795A (zh) 2019-08-22 2022-04-15 巴斯夫欧洲公司 淀粉酶变体
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114835780B (zh) * 2022-06-29 2022-09-27 中国农业大学 辣椒籽分离的寡肽medei及其在预防或治疗癌症中的应用

Family Cites Families (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US87571A (en) 1869-03-09 Improved axle-set
SE318048B (pt) 1959-09-21 1969-12-01 Pellerin Ab Zenith
SE417441B (sv) 1977-08-19 1981-03-16 Kare Larsson Forfarande for att isolera polera lipider ur en blandning av polera och opolera cerealiclipider genom blandning med vatten och derpa foljande tyngdkraftsseparation
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4707364A (en) * 1984-01-27 1987-11-17 Miles Laboratories, Inc. Composition for accelerating cheese aging
US5087571A (en) 1984-06-22 1992-02-11 President And Fellows Of Harvard College Method for providing a cell culture from a transgenic non-human mammal
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0590689B2 (fr) 1985-03-30 2006-08-16 KAUFFMAN, Stuart A. Procédé d'obtention d'ADN, ARN, peptides, polypeptides ou protéines, par une technique de recombinaison d'ADN
US4752483A (en) * 1986-05-30 1988-06-21 Campbell Soup Company Method for producing a highly flavored cheese ingredient
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5573933A (en) 1987-04-14 1996-11-12 Luminis Pty, Ltd. Transgenic pigs
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5217879A (en) 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5326477A (en) 1990-05-07 1994-07-05 Bio-Sep, Inc. Process for digesting solid waste
ES2217250T3 (es) 1990-06-15 2004-11-01 Scios Inc. Mamifero transgenico, no humano que muestra la patologia de formacion amiloides de la enfermedad de alzheimer.
EP0558676B1 (en) 1990-11-23 2000-04-19 Plant Genetic Systems, N.V. Process for transforming monocotyledonous plants
ATE177782T1 (de) 1990-12-20 1999-04-15 Ixsys Inc Optimierung von bindenden proteinen
US6110700A (en) 1991-03-11 2000-08-29 The General Hospital Corporation PRAD1 cyclin and its cDNA
DE4115938A1 (de) 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
US5922854A (en) 1991-06-14 1999-07-13 Kumar; Ramesh Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids
CA2075135A1 (en) 1991-08-02 1993-02-03 David E. Ellis Particle-mediated transformation of gymnosperms
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
JP2626380B2 (ja) 1991-11-12 1997-07-02 株式会社大林組 クレーン用吊治具の旋回防止装置
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
JPH06306386A (ja) 1993-04-25 1994-11-01 Showa Sangyo Co Ltd 油脂の精製方法
JP2937746B2 (ja) 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法
JPH08509871A (ja) 1993-09-30 1996-10-22 アグラシータス インコーポレイテッド 異種ペルオキシダーゼを生産するトランスジェニック綿植物
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
DE4339556C1 (de) 1993-11-19 1995-02-02 Metallgesellschaft Ag Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5639940A (en) 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
CA2184688A1 (en) 1994-03-09 1995-09-14 Pedro Antonio Prieto Transgenic animals producing oligosaccharides and glycoconjugates
AU694181B2 (en) 1994-03-09 1998-07-16 Abbott Laboratories Humanized milk
CN1326994C (zh) 1994-03-29 2007-07-18 诺沃奇梅兹有限公司 碱性芽孢杆菌淀粉酶
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5959171A (en) 1994-08-17 1999-09-28 Pharming B.V. Method for the production of biologically active polypeptides in a mammal's
CA2198451A1 (en) 1994-09-01 1996-03-07 Gurparkash Singh Transgenic animal expressing a familial form of human amyloid precursor protein
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US6187992B1 (en) 1994-12-05 2001-02-13 Merck & Co., Inc. Transgenic mouse having a disrupted amyloid precursor protein gene
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
CA2223103A1 (en) 1995-06-06 1996-12-12 Isis Pharmaceuticals Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
CA2225475A1 (en) 1995-06-27 1997-01-16 Unilever Plc Immobilized enzyme and its use for the processing of triglyceride oils
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
DE19527274A1 (de) 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
SE504664C2 (sv) 1995-09-22 1997-03-24 Scotia Lipidteknik Ab Sätt att framställa fraktionerad olja, oljan, dess användning samt emulsionskomposition innehållande oljan
WO1997016533A1 (en) 1995-10-31 1997-05-09 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6022963A (en) 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
US6057100A (en) 1996-06-07 2000-05-02 Eos Biotechnology, Inc. Oligonucleotide arrays
US6326341B1 (en) 1996-09-11 2001-12-04 The Procter & Gamble Company Low foaming automatic dishwashing compositions
US6127137A (en) 1996-10-31 2000-10-03 Novo Nordisk A/S Acidic phospholipase, production and methods using thereof
CN1235636A (zh) 1996-10-31 1999-11-17 诺沃挪第克公司 新型磷脂酶,及其生产和应用
DK0869167T4 (da) 1996-12-09 2010-03-08 Novozymes As Reduktion af phosphor-indeholdende bestanddele i spiseolier; som omfatter en stor mængde ikke-hydrerbart phosphor, ved anvendelse af en phospholipase, en phospholipase fra en trådsvamp, der har en phospholipase A og/eller B aktivitet
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
CZ221899A3 (cs) 1996-12-19 1999-11-17 Unilever N. V. Způsob imobilizace enzymu
US6069122A (en) 1997-06-16 2000-05-30 The Procter & Gamble Company Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution
AU743305C (en) 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US6303803B1 (en) 1997-01-31 2001-10-16 Cargill, Incorporated Removal of sterols from fats and oils
US5880300A (en) 1997-01-31 1999-03-09 Cargill, Incorporated Phospholipid-based removal of sterols from fats and oils
ATE399880T1 (de) 1997-03-18 2008-07-15 Novozymes As Ein in-vitro-verfahren zur herstellung einer dna- bibliothek
EP0996718B1 (en) 1997-03-18 2008-12-24 Novozymes A/S Method for constructing a library using dna shuffling
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6826296B2 (en) 1997-07-25 2004-11-30 Affymetrix, Inc. Method and system for providing a probe array chip design database
AU8908198A (en) 1997-08-15 1999-03-08 Hyseq, Inc. Methods and compositions for detection or quantification of nucleic acid species
AU733589B2 (en) 1997-09-11 2001-05-17 Bioventures, Inc. Method of making high density arrays
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6399383B1 (en) 1997-10-28 2002-06-04 Maxygen, Inc. Human papilloma virus vectors
CA2308119C (en) 1997-10-30 2014-06-03 Novo Nordisk A/S .alpha.-amylase mutants
WO1999023107A1 (en) 1997-10-31 1999-05-14 Maxygen, Incorporated Modification of virus tropism and host range by viral genome shuffling
ATE234913T1 (de) 1997-11-10 2003-04-15 Procter & Gamble Verfahren zur herstellung einer waschmitteltablette
EP0921189B1 (en) 1997-11-14 2005-01-12 Sankyo Company Limited Transgenic animal allergy models and methods for their use
WO1999029902A1 (en) 1997-12-08 1999-06-17 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
JP2002507393A (ja) 1998-02-11 2002-03-12 マキシジェン, インコーポレイテッド 抗原ライブラリー免疫
JP2002507392A (ja) 1998-02-11 2002-03-12 マキシジェン, インコーポレイテッド 遺伝子ワクチンの免疫調節特性の最適化
CA2323638A1 (en) 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
US6156952A (en) 1998-04-09 2000-12-05 Constituent Institution Of The University Of Maryland System HIV transgenic animals and uses therefor
WO1999057360A1 (en) 1998-05-01 1999-11-11 Novo Nordisk A/S Enhancers such as n-hydroxyacetanilide
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
DE19824705A1 (de) 1998-06-03 1999-12-09 Henkel Kgaa Amylase und Protease enthaltende Wasch- und Reinigungsmittel
JP4700805B2 (ja) 1998-06-29 2011-06-15 ブリストル−マイヤーズ スクウィブ カンパニー 多様性の高いライブラリーを製造する方法
JP4335995B2 (ja) 1998-07-22 2009-09-30 昭 神谷 環境保全型粒状洗浄用組成物
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US6172248B1 (en) * 1998-11-20 2001-01-09 Ip Holdings, L.L.C. Methods for refining vegetable oils and byproducts thereof
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
US6511824B1 (en) 1999-03-17 2003-01-28 Exelixis, Inc. Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use
US6309871B1 (en) 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
EP1106603A3 (en) 1999-12-06 2003-11-19 Fuji Photo Film Co., Ltd. DNA chip and reactive solid carrier
US6531644B1 (en) 2000-01-14 2003-03-11 Exelixis, Inc. Methods for identifying anti-cancer drug targets
AU2002211665B2 (en) 2000-10-12 2005-10-20 Exelixis, Inc. Human Ect2 and methods of use
US6309872B1 (en) 2000-11-01 2001-10-30 Novozymes Biotech, Inc Polypeptides having glucoamylase activity and nucleic acids encoding same
JP6449266B2 (ja) 2013-06-21 2019-01-09 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 流体ピックアップを備えるマイクロ流体システム

Also Published As

Publication number Publication date
EA200401407A1 (ru) 2006-02-24
AU2003243157C1 (en) 2008-09-04
EA010903B1 (ru) 2008-12-30
EP1497418A2 (en) 2005-01-19
WO2003089620A2 (en) 2003-10-30
EP1497418B1 (en) 2012-10-17
CA2481411A1 (en) 2003-10-30
ES2395898T3 (es) 2013-02-15
PT1497418E (pt) 2013-01-25
US20040005604A1 (en) 2004-01-08
BRPI0309391B1 (pt) 2018-10-30
EP2298871A1 (en) 2011-03-23
CN1659276A (zh) 2005-08-24
AU2003243157A1 (en) 2003-11-03
EP1497418A4 (en) 2006-02-01
CA2481411C (en) 2016-06-14
CN102174546A (zh) 2011-09-07
WO2003089620A3 (en) 2004-10-14
AU2003243157B2 (en) 2008-03-20
JP2005523019A (ja) 2005-08-04

Similar Documents

Publication Publication Date Title
BR0309391A (pt) Fosfolipases, ácidos nucléicos codificando-as e métodos para preparar e usá-las
BRPI0508559A (pt) fosfolipases, ácidos nucléicos codificando-os e métodos para preparar e empregá-los
EA200970308A1 (ru) Фосфолипазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
PT1312922E (pt) Composicoes de reagente de tetrazolio estabilizadas com nitrito e metodos para a utilizacao das mesmas
AR050254A1 (es) Intermediarios y procesos para preparar dichos intermediarios utiles en la fabricacion de pirrolotriazinas
ATE464795T1 (de) Würzige öl-in-wasser-emulsionen für nahrungsmittel anwendugen
DE60139262D1 (de) System zur wiederherstellung von gelenkoberflächen von säugetieren
DK1268847T3 (da) Ubiquitin-ligaseassay
DE59803230D1 (de) Verfahren zur auswertung des brennraumdruckverlaufs
ATE307804T1 (de) Effiziente durch liganden vermittelte ullmann kupplung von anilinen und azolen
ATE417092T1 (de) Reinigungs- und desinfektionsmittel für harte oberflächen
PA8526301A1 (es) Productos alimenticios para la vida acuatica y metodo de alimentacion para la misma
WO2002066653A3 (en) Procaryotic libraries and uses
DE60214537D1 (de) Entfernung von ölen aus säuren
IT1291123B1 (it) Micropompa perfezionata per la nebulizzazione di fluidi
BR0203933B1 (pt) aparelho abastecedor de óleo para compressor alternado do tipo oposto.
DE69934325D1 (de) Zusammenstellungen zur erhöhung der darmabsoption von fetten
DE60215031D1 (de) Verbesserungen von behältern für scharfe gegenstände
ATE346087T1 (de) Hepatitis e-virus aus dem schwein und dessen verwendungen
BR0010191A (pt) Determinação de estruturas de fosfolipìdio/lipìdio complexas com auxìlio de acilglicerìdeos marcados com fluorescência sintéticos
DE69525813D1 (de) Regulator-Gen für die Expression vor Nitril-Hydratase
ATE270551T1 (de) Kombinationspräparat aus 2-methylthiazolidin-2,4- dicarbonsäure und paracetamol
ATA4698A (de) Anordnung zur ermittlung der stückzahl von presseerzeugnissen
TH138527A (pt)
ITUD20000177A0 (it) Dispositivo di variazione posizionamento cilindri di laminazione per prodotti piani

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B25D Requested change of name of applicant approved

Owner name: VERENIUM CORPORATION (US)

Free format text: NOME ALTERADO DE: DIVERSA CORPORATION

B25A Requested transfer of rights approved

Owner name: DSM FOOD SPECIALTIES B.V (NL)

B25A Requested transfer of rights approved

Owner name: DSM IP ASSETS B.V. (NL)

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 30/10/2018, OBSERVADAS AS CONDICOES LEGAIS.